{"id":"https://openalex.org/W2166909912","doi":null,"title":"PresequenceProtease (PreP), a novel Peptidasome in Mitochondria and Chloroplasts: Localization, Function, Structure and Mechanism of Proteolysis","display_name":"PresequenceProtease (PreP), a novel Peptidasome in Mitochondria and Chloroplasts: Localization, Function, Structure and Mechanism of Proteolysis","publication_year":2007,"publication_date":"2007-01-01","ids":{"openalex":"https://openalex.org/W2166909912","mag":"2166909912"},"language":"en","primary_location":{"id":"pmh:oai:DiVA.org:su-6869","doi":null,"is_oa":true,"landing_page_url":"http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-6869","pdf_url":null,"source":{"id":"https://openalex.org/S4306401559","display_name":"KTH Publication Database DiVA (KTH Royal Institute of Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I86987016","host_organization_name":"KTH Royal Institute of Technology","host_organization_lineage":["https://openalex.org/I86987016"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"info:eu-repo/semantics/doctoralThesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-6869","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5080863590","display_name":"Shashi Bhushan","orcid":"https://orcid.org/0000-0002-8514-5959","observed_orcids":["https://orcid.org/0000-0002-8514-5959"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Shashi Bhushan","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-8514-5959","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5080863590"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.36959999799728394,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.36959999799728394,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10620","display_name":"Protease and Inhibitor Mechanisms","score":0.3391000032424927,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11041","display_name":"Ubiquitin and proteasome pathways","score":0.16500000655651093,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dual-targeting","display_name":"dual targeting","score":0.5400000214576721},{"id":"https://openalex.org/keywords/arabidopsis-thaliana","display_name":"Arabidopsis thaliana","score":0.828000009059906},{"id":"https://openalex.org/keywords/crystal-structure","display_name":"crystal structure","score":0.6769999861717224},{"id":"https://openalex.org/keywords/protease-mechanism","display_name":"protease mechanism","score":0.5910000205039978},{"id":"https://openalex.org/keywords/substrate-specificity","display_name":"substrate specificity","score":0.7620000243186951},{"id":"https://openalex.org/keywords/amyloid-beta-degradation","display_name":"amyloid beta degradation","score":0.7789999842643738},{"id":"https://openalex.org/keywords/alzheimer-s-disease","display_name":"Alzheimer's disease","score":0.9359999895095825},{"id":"https://openalex.org/keywords/mitochondrial-proteolysis","display_name":"mitochondrial proteolysis","score":0.7310000061988831}],"concepts":[{"id":"https://openalex.org/C2781307694","wikidata":"https://www.wikidata.org/wiki/Q33123","display_name":"Proteolysis","level":3,"score":0.8633962273597717},{"id":"https://openalex.org/C2776714187","wikidata":"https://www.wikidata.org/wiki/Q212410","display_name":"Protease","level":3,"score":0.5834527611732483},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5585727095603943},{"id":"https://openalex.org/C2779281246","wikidata":"https://www.wikidata.org/wiki/Q172847","display_name":"Peptide","level":2,"score":0.5585569739341736},{"id":"https://openalex.org/C69305403","wikidata":"https://www.wikidata.org/wiki/Q47263","display_name":"Chloroplast","level":3,"score":0.523014485836029},{"id":"https://openalex.org/C168240541","wikidata":"https://www.wikidata.org/wiki/Q45948","display_name":"Organelle","level":2,"score":0.5228438973426819},{"id":"https://openalex.org/C28859421","wikidata":"https://www.wikidata.org/wiki/Q39572","display_name":"Mitochondrion","level":2,"score":0.5161781907081604},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.44616690278053284},{"id":"https://openalex.org/C53345823","wikidata":"https://www.wikidata.org/wiki/Q5531554","display_name":"Gene isoform","level":3,"score":0.43122392892837524},{"id":"https://openalex.org/C2779491563","wikidata":"https://www.wikidata.org/wiki/Q157892","display_name":"Arabidopsis","level":4,"score":0.4228658080101013},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.42221519351005554},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3823995292186737},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.1729089915752411},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.08285519480705261},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.05639609694480896}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:DiVA.org:su-6869","doi":null,"is_oa":true,"landing_page_url":"http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-6869","pdf_url":null,"source":{"id":"https://openalex.org/S4306401559","display_name":"KTH Publication Database DiVA (KTH Royal Institute of Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I86987016","host_organization_name":"KTH Royal Institute of Technology","host_organization_lineage":["https://openalex.org/I86987016"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"info:eu-repo/semantics/doctoralThesis"},{"id":"mag:2166909912","doi":null,"is_oa":false,"landing_page_url":"http://diva-portal.org/smash/get/diva2:197228/fulltext01.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:DiVA.org:su-6869","doi":null,"is_oa":true,"landing_page_url":"http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-6869","pdf_url":null,"source":{"id":"https://openalex.org/S4306401559","display_name":"KTH Publication Database DiVA (KTH Royal Institute of Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I86987016","host_organization_name":"KTH Royal Institute of Technology","host_organization_lineage":["https://openalex.org/I86987016"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"info:eu-repo/semantics/doctoralThesis"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":306,"referenced_works":["https://openalex.org/W7932018","https://openalex.org/W28074342","https://openalex.org/W38728615","https://openalex.org/W40646405","https://openalex.org/W111354659","https://openalex.org/W126822491","https://openalex.org/W259119803","https://openalex.org/W328114118","https://openalex.org/W1483685144","https://openalex.org/W1493498138","https://openalex.org/W1499660611","https://openalex.org/W1508802547","https://openalex.org/W1510176560","https://openalex.org/W1515484373","https://openalex.org/W1517989168","https://openalex.org/W1527717785","https://openalex.org/W1533931073","https://openalex.org/W1543063876","https://openalex.org/W1551504358","https://openalex.org/W1557879033","https://openalex.org/W1569904835","https://openalex.org/W1573941143","https://openalex.org/W1580184135","https://openalex.org/W1598966730","https://openalex.org/W1599740626","https://openalex.org/W1600835334","https://openalex.org/W1608825978","https://openalex.org/W1656671666","https://openalex.org/W1675293333","https://openalex.org/W1687309826","https://openalex.org/W1740666950","https://openalex.org/W1755765541","https://openalex.org/W1821016817","https://openalex.org/W1859256729","https://openalex.org/W1879947423","https://openalex.org/W1897988095","https://openalex.org/W1901311969","https://openalex.org/W1943336505","https://openalex.org/W1964383600","https://openalex.org/W1964985775","https://openalex.org/W1966165014","https://openalex.org/W1968038037","https://openalex.org/W1968804110","https://openalex.org/W1969250063","https://openalex.org/W1969407643","https://openalex.org/W1969982045","https://openalex.org/W1972008234","https://openalex.org/W1972499484","https://openalex.org/W1973794441","https://openalex.org/W1974278342","https://openalex.org/W1974877324","https://openalex.org/W1975460230","https://openalex.org/W1975656610","https://openalex.org/W1978125035","https://openalex.org/W1978586782","https://openalex.org/W1979489548","https://openalex.org/W1980527941","https://openalex.org/W1980637648","https://openalex.org/W1983299252","https://openalex.org/W1984545749","https://openalex.org/W1984785948","https://openalex.org/W1985296992","https://openalex.org/W1985634923","https://openalex.org/W1988348515","https://openalex.org/W1988601980","https://openalex.org/W1988934693","https://openalex.org/W1989644766","https://openalex.org/W1990670446","https://openalex.org/W1991174372","https://openalex.org/W1991345254","https://openalex.org/W1992923868","https://openalex.org/W1995363256","https://openalex.org/W1995630780","https://openalex.org/W1996097229","https://openalex.org/W1996476714","https://openalex.org/W1996672716","https://openalex.org/W1996715099","https://openalex.org/W1997577244","https://openalex.org/W1998053020","https://openalex.org/W1999222952","https://openalex.org/W1999778386","https://openalex.org/W2002454175","https://openalex.org/W2003076028","https://openalex.org/W2004747515","https://openalex.org/W2005040944","https://openalex.org/W2005521382","https://openalex.org/W2006118708","https://openalex.org/W2006473139","https://openalex.org/W2006548069","https://openalex.org/W2008266189","https://openalex.org/W2009609492","https://openalex.org/W2009680690","https://openalex.org/W2010283986","https://openalex.org/W2010526388","https://openalex.org/W2011858193","https://openalex.org/W2012842274","https://openalex.org/W2012938147","https://openalex.org/W2013293668","https://openalex.org/W2016181418","https://openalex.org/W2016355476","https://openalex.org/W2016531329","https://openalex.org/W2018329098","https://openalex.org/W2018363274","https://openalex.org/W2018502225","https://openalex.org/W2019247734","https://openalex.org/W2020197143","https://openalex.org/W2021556583","https://openalex.org/W2022114463","https://openalex.org/W2022567869","https://openalex.org/W2023531011","https://openalex.org/W2023675452","https://openalex.org/W2024182748","https://openalex.org/W2024590669","https://openalex.org/W2026169768","https://openalex.org/W2026197201","https://openalex.org/W2027617079","https://openalex.org/W2029152058","https://openalex.org/W2029331292","https://openalex.org/W2029502542","https://openalex.org/W2030821334","https://openalex.org/W2032226202","https://openalex.org/W2032284161","https://openalex.org/W2032474120","https://openalex.org/W2032889785","https://openalex.org/W2034621386","https://openalex.org/W2035663056","https://openalex.org/W2036794157","https://openalex.org/W2037954483","https://openalex.org/W2039276913","https://openalex.org/W2039340205","https://openalex.org/W2039492407","https://openalex.org/W2039718087","https://openalex.org/W2040076595","https://openalex.org/W2042397530","https://openalex.org/W2043433011","https://openalex.org/W2043514114","https://openalex.org/W2044895959","https://openalex.org/W2044977183","https://openalex.org/W2045375219","https://openalex.org/W2046353892","https://openalex.org/W2046446960","https://openalex.org/W2046495643","https://openalex.org/W2047765304","https://openalex.org/W2047841805","https://openalex.org/W2047930189","https://openalex.org/W2048139850","https://openalex.org/W2049467526","https://openalex.org/W2049560931","https://openalex.org/W2050627714","https://openalex.org/W2051141018","https://openalex.org/W2051514994","https://openalex.org/W2052563180","https://openalex.org/W2053684471","https://openalex.org/W2054488866","https://openalex.org/W2054752741","https://openalex.org/W2055684980","https://openalex.org/W2055798417","https://openalex.org/W2055917361","https://openalex.org/W2056099910","https://openalex.org/W2056880208","https://openalex.org/W2057842451","https://openalex.org/W2059426360","https://openalex.org/W2059725953","https://openalex.org/W2060399663","https://openalex.org/W2067356770","https://openalex.org/W2067359407","https://openalex.org/W2067511286","https://openalex.org/W2068422582","https://openalex.org/W2068619217","https://openalex.org/W2069490007","https://openalex.org/W2070264340","https://openalex.org/W2070343649","https://openalex.org/W2070420713","https://openalex.org/W2071030118","https://openalex.org/W2071484180","https://openalex.org/W2072256827","https://openalex.org/W2073554351","https://openalex.org/W2073984835","https://openalex.org/W2074097929","https://openalex.org/W2074790737","https://openalex.org/W2075568856","https://openalex.org/W2075618717","https://openalex.org/W2076473989","https://openalex.org/W2077307563","https://openalex.org/W2077574127","https://openalex.org/W2079093410","https://openalex.org/W2079202691","https://openalex.org/W2079676584","https://openalex.org/W2080195140","https://openalex.org/W2080295094","https://openalex.org/W2081598019","https://openalex.org/W2082513753","https://openalex.org/W2082737976","https://openalex.org/W2086624766","https://openalex.org/W2087579231","https://openalex.org/W2087669983","https://openalex.org/W2088419874","https://openalex.org/W2088449512","https://openalex.org/W2088860711","https://openalex.org/W2089327099","https://openalex.org/W2089884985","https://openalex.org/W2091575008","https://openalex.org/W2091586560","https://openalex.org/W2092522921","https://openalex.org/W2092705523","https://openalex.org/W2093769391","https://openalex.org/W2094093683","https://openalex.org/W2095355512","https://openalex.org/W2096012822","https://openalex.org/W2096457861","https://openalex.org/W2096624106","https://openalex.org/W2096937628","https://openalex.org/W2097817417","https://openalex.org/W2098491117","https://openalex.org/W2099733017","https://openalex.org/W2100544480","https://openalex.org/W2103024032","https://openalex.org/W2103313412","https://openalex.org/W2104178065","https://openalex.org/W2105251326","https://openalex.org/W2108793598","https://openalex.org/W2109393332","https://openalex.org/W2109502920","https://openalex.org/W2110632298","https://openalex.org/W2110771249","https://openalex.org/W2110958932","https://openalex.org/W2111976416","https://openalex.org/W2112059525","https://openalex.org/W2114341896","https://openalex.org/W2114358611","https://openalex.org/W2114449891","https://openalex.org/W2114796662","https://openalex.org/W2115123145","https://openalex.org/W2116817481","https://openalex.org/W2117335544","https://openalex.org/W2119415618","https://openalex.org/W2119624493","https://openalex.org/W2119681168","https://openalex.org/W2120363574","https://openalex.org/W2121406152","https://openalex.org/W2122351685","https://openalex.org/W2122930401","https://openalex.org/W2123369982","https://openalex.org/W2126376871","https://openalex.org/W2127178684","https://openalex.org/W2128319845","https://openalex.org/W2129095580","https://openalex.org/W2129231604","https://openalex.org/W2129526881","https://openalex.org/W2129618180","https://openalex.org/W2130190479","https://openalex.org/W2131192895","https://openalex.org/W2131541394","https://openalex.org/W2132814291","https://openalex.org/W2132962532","https://openalex.org/W2132988134","https://openalex.org/W2133757350","https://openalex.org/W2134915935","https://openalex.org/W2135166311","https://openalex.org/W2136195150","https://openalex.org/W2137158408","https://openalex.org/W2137418955","https://openalex.org/W2137729056","https://openalex.org/W2138041386","https://openalex.org/W2138881473","https://openalex.org/W2139514626","https://openalex.org/W2140450495","https://openalex.org/W2141594388","https://openalex.org/W2143688472","https://openalex.org/W2144147614","https://openalex.org/W2144283041","https://openalex.org/W2144458008","https://openalex.org/W2145571109","https://openalex.org/W2146017566","https://openalex.org/W2147844147","https://openalex.org/W2148084260","https://openalex.org/W2148626789","https://openalex.org/W2148664814","https://openalex.org/W2149015744","https://openalex.org/W2149150386","https://openalex.org/W2150164439","https://openalex.org/W2150689878","https://openalex.org/W2150984321","https://openalex.org/W2151380136","https://openalex.org/W2152100426","https://openalex.org/W2152458080","https://openalex.org/W2152926079","https://openalex.org/W2155015138","https://openalex.org/W2155054459","https://openalex.org/W2155750288","https://openalex.org/W2162856464","https://openalex.org/W2163069454","https://openalex.org/W2163757074","https://openalex.org/W2164936978","https://openalex.org/W2165202914","https://openalex.org/W2165395680","https://openalex.org/W2165647387","https://openalex.org/W2165922683","https://openalex.org/W2167379849","https://openalex.org/W2167961569","https://openalex.org/W2168291501","https://openalex.org/W2170621128","https://openalex.org/W2170806773","https://openalex.org/W2179149811","https://openalex.org/W2605346087","https://openalex.org/W2615749116"],"related_works":["https://openalex.org/W2090754360","https://openalex.org/W218334477","https://openalex.org/W2060143133","https://openalex.org/W2041614322","https://openalex.org/W4412590437","https://openalex.org/W2102476265","https://openalex.org/W1984785948","https://openalex.org/W2066855708","https://openalex.org/W1969118834","https://openalex.org/W2033309752"],"abstract_inverted_index":{"The":[0,23,64,149,163,179],"information":[1],"for":[2,122,207,251],"mitochondrial":[3,123],"and":[4,8,94,99,104,124,130,141],"chloroplastic":[5,125],"protein":[6],"import":[7,35,48],"targeting":[9,21,24,41,76,107,116],"generally":[10],"resides":[11],"in":[12,134,136,183,198,214,220,241],"the":[13,17,30,45,62,73,80,87,109,128,153,166,176,200,248],"N-terminal":[14],"part":[15],"of":[16,36,66,90,108,152,172,192,228,243,253],"protein,":[18],"called":[19],"a":[20,37,119,173,184,189,224],"peptide.":[22,256],"peptide":[25,77,117],"is":[26,111,209,239,247],"cleaved":[27],"off":[28],"by":[29,52],"organellar":[31],"processing":[32],"peptidases":[33],"after":[34,47],"precursor":[38],"protein.":[39],"Free":[40],"peptides":[42],"generated":[43],"inside":[44],"organelle":[46],"are":[49,96,132],"rapidly":[50],"degraded":[51],"proteolysis":[53,208],"as":[54],"their":[55],"accumulation":[56],"can":[57],"have":[58,84,143],"toxic":[59,255],"effects":[60],"on":[61],"organelle.":[63],"aim":[65],"this":[67,254],"thesis":[68],"has":[69,223],"been":[70],"to":[71,101,113,216],"investigate":[72],"newly":[74],"identified":[75],"degrading":[78],"protease,":[79],"PresequenceProtease":[81],"(PreP).":[82],"We":[83,232],"shown":[85],"that":[86,234],"two":[88],"isoforms":[89],"Arabidopsis":[91,135],"PreP":[92,180,219,246],"(AtPreP1":[93],"AtPreP1)":[95],"dually":[97],"targeted":[98],"localized":[100],"both":[102],"mitochondria":[103,222,242],"chloroplasts.":[105],"Dual":[106],"AtPreP1":[110,129,155],"due":[112],"an":[114,137],"ambiguous":[115],"with":[118],"domain":[120],"organization":[121],"targeting.":[126],"Both":[127],"AtPreP2":[131],"expressed":[133],"organ":[138],"specific":[139],"manner":[140],"they":[142],"distinct":[144],"but":[145],"overlapping":[146],"substrate":[147,168,217],"specificity.":[148],"crystal":[150],"structure":[151,164],"recombinant":[154],"E80Q":[156],"was":[157],"solved":[158],"at":[159],"2.1":[160],"\u00c5":[161],"resolution.":[162],"represents":[165],"first":[167],"bound,":[169],"closed":[170],"conformation":[171],"protease":[174,249],"from":[175],"pitrilysin":[177],"family.":[178],"polypeptide":[181],"folds":[182],"unique":[185],"peptidasome":[186],"structure,":[187],"surrounding":[188],"huge":[190],"cavity":[191],"more":[193],"than":[194],"10":[195],"000":[196],"\u00c53":[197],"which":[199],"active":[201],"site":[202],"resides.":[203],"A":[204],"novel":[205,225],"mechanism":[206],"proposed":[210],"involving":[211],"hinge-bending":[212],"motions":[213],"response":[215],"binding.":[218],"human":[221],"function:":[226],"degradation":[227,252],"amyloid":[229],"\u03b2-peptide":[230],"(A\u03b2).":[231],"show":[233],"under":[235],"circumstances":[236],"when":[237],"A\u03b2":[238],"present":[240],"Alzheimer\u2019s":[244],"patients,":[245],"responsible":[250]},"counts_by_year":[],"updated_date":"2026-08-13T07:04:57.449891","created_date":"2016-06-24T00:00:00"}