{"id":"https://openalex.org/W2166867387","doi":"https://doi.org/10.1139/b76-125","title":"Isolation and partial characterization of cytoplasmic and wall-bound acid phosphatases from wheat roots","display_name":"Isolation and partial characterization of cytoplasmic and wall-bound acid phosphatases from wheat roots","publication_year":1976,"publication_date":"1976-05-15","ids":{"openalex":"https://openalex.org/W2166867387","doi":"https://doi.org/10.1139/b76-125","mag":"2166867387"},"language":"en","primary_location":{"id":"doi:10.1139/b76-125","doi":"https://doi.org/10.1139/b76-125","is_oa":false,"landing_page_url":"https://doi.org/10.1139/b76-125","pdf_url":null,"source":{"id":"https://openalex.org/S4210188060","display_name":"Canadian Journal of Botany","issn_l":"0008-4026","issn":["0008-4026","1480-3305"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","norway-1"],"host_organization":"https://openalex.org/P4310312929","host_organization_name":"Canadian Science Publishing","host_organization_lineage":["https://openalex.org/P4310312929"],"host_organization_lineage_names":["Canadian Science Publishing"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Canadian Journal of Botany","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Yoichi Hasegawa","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Yoichi Hasegawa","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5104858026","display_name":"Kenneth R. Lynn","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"K. R. Lynn","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5036590416","display_name":"W. James Brockbank","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"W. James Brockbank","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.7408,"has_fulltext":false,"cited_by_count":43,"citation_normalized_percentile":{"value":0.66199061,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":95},"biblio":{"volume":"54","issue":"10","first_page":"1163","last_page":"1169"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11247","display_name":"Plant nutrient uptake and metabolism","score":0.9907000064849854,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11247","display_name":"Plant nutrient uptake and metabolism","score":0.9907000064849854,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10869","display_name":"Polysaccharides and Plant Cell Walls","score":0.00279999990016222,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12991","display_name":"Phytase and its Applications","score":0.0017999999690800905,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/acid-phosphatase","display_name":"acid phosphatase","score":0.9940000176429749},{"id":"https://openalex.org/keywords/wheat-roots","display_name":"wheat roots","score":0.9679999947547913},{"id":"https://openalex.org/keywords/wheat","display_name":"wheat","score":0.9110000133514404},{"id":"https://openalex.org/keywords/cell-wall-bound-enzymes","display_name":"cell wall-bound enzymes","score":0.7710000276565552},{"id":"https://openalex.org/keywords/cytoplasmic-enzymes","display_name":"cytosolic enzyme","score":0.9909999966621399},{"id":"https://openalex.org/keywords/enzyme-purification","display_name":"enzyme purification","score":0.6679999828338623},{"id":"https://openalex.org/keywords/substrate-specificity","display_name":"substrate specificity","score":0.9269999861717224},{"id":"https://openalex.org/keywords/enzyme-inhibition","display_name":"enzyme inhibition","score":0.6779999732971191},{"id":"https://openalex.org/keywords/metal-ion-effects","display_name":"metal ion effects","score":0.6380000114440918},{"id":"https://openalex.org/keywords/molecular-weight","display_name":"molecular weight","score":0.5419999957084656},{"id":"https://openalex.org/keywords/root-cell-wall","display_name":"root cell wall","score":0.3569999933242798}],"concepts":[{"id":"https://openalex.org/C163994747","wikidata":"https://www.wikidata.org/wiki/Q2916358","display_name":"Sephadex","level":3,"score":0.8145594000816345},{"id":"https://openalex.org/C193363816","wikidata":"https://www.wikidata.org/wiki/Q1617865","display_name":"Size-exclusion chromatography","level":3,"score":0.7078933715820312},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6911096572875977},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.6851083636283875},{"id":"https://openalex.org/C2777132085","wikidata":"https://www.wikidata.org/wiki/Q46220103","display_name":"Phosphate","level":2,"score":0.6461445093154907},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5971542000770569},{"id":"https://openalex.org/C178666793","wikidata":"https://www.wikidata.org/wiki/Q422476","display_name":"Phosphatase","level":3,"score":0.5293850302696228},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.4914158582687378},{"id":"https://openalex.org/C125235067","wikidata":"https://www.wikidata.org/wiki/Q128700","display_name":"Cell wall","level":2,"score":0.48506131768226624},{"id":"https://openalex.org/C21547065","wikidata":"https://www.wikidata.org/wiki/Q418243","display_name":"Acid phosphatase","level":3,"score":0.45715826749801636},{"id":"https://openalex.org/C58121356","wikidata":"https://www.wikidata.org/wiki/Q182854","display_name":"Molecular mass","level":3,"score":0.44273439049720764},{"id":"https://openalex.org/C537181965","wikidata":"https://www.wikidata.org/wiki/Q658","display_name":"Sodium","level":2,"score":0.4369739592075348},{"id":"https://openalex.org/C2776081644","wikidata":"https://www.wikidata.org/wiki/Q411030","display_name":"Carboxymethyl cellulose","level":3,"score":0.4177207350730896},{"id":"https://openalex.org/C190062978","wikidata":"https://www.wikidata.org/wiki/Q79899","display_name":"Cytoplasm","level":2,"score":0.4142727553844452},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1139/b76-125","doi":"https://doi.org/10.1139/b76-125","is_oa":false,"landing_page_url":"https://doi.org/10.1139/b76-125","pdf_url":null,"source":{"id":"https://openalex.org/S4210188060","display_name":"Canadian Journal of Botany","issn_l":"0008-4026","issn":["0008-4026","1480-3305"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","norway-1"],"host_organization":"https://openalex.org/P4310312929","host_organization_name":"Canadian Science Publishing","host_organization_lineage":["https://openalex.org/P4310312929"],"host_organization_lineage_names":["Canadian Science Publishing"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Canadian Journal of Botany","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2133985273","https://openalex.org/W1835296828","https://openalex.org/W2061534515","https://openalex.org/W2096897016","https://openalex.org/W1982030517","https://openalex.org/W2064228729","https://openalex.org/W1969135423","https://openalex.org/W1828598372","https://openalex.org/W2049867534","https://openalex.org/W2096467079"],"abstract_inverted_index":{"In":[0],"wheat":[1],"(Triticum":[2],"aestivum)":[3],"roots,":[4],"about":[5],"67%":[6],"of":[7,11,23,63,119],"the":[8,17,28,73,120,132],"total":[9],"activity":[10,87,100],"acid":[12,44],"phosphatase":[13],"was":[14,25],"associated":[15],"with":[16,32],"cell":[18],"wall":[19,29],"debris,":[20],"and":[21,40,55,61,78,94,97,105,115,125,143,155],"35%":[22],"it":[24],"released":[26],"from":[27,170],"by":[30,49,91,141,149,164],"incubation":[31],"0.8":[33],"M":[34],"NaCl":[35],"overnight.":[36],"Three":[37],"major":[38,42],"cytoplasmic":[39],"two":[41],"wall-bound":[43,108],"phosphatases":[45],"were":[46,66,110,138],"then":[47],"separated":[48],"gel":[50,167],"filtration":[51],"on":[52,58,131],"Sephadex":[53],"G-75":[54],"ion-exchange":[56],"chromatography":[57],"carboxymethyl":[59],"cellulose,":[60],"some":[62],"their":[64],"characteristics":[65],"compared.":[67],"They":[68,83],"showed":[69,85],"maximum":[70],"activities":[71],"in":[72,157],"same":[74],"pH":[75],"range":[76],"(4.7\u20135.0)":[77],"had":[79,127],"broad":[80],"substrate":[81],"specificities.":[82],"all":[84,136],"high":[86],"toward":[88,101],"ADP.":[89],"followed":[90],"ATP,":[92],"glucose-6-phosphate,":[93],"creatine":[95],"phosphate":[96],"much":[98],"less":[99],"AMP,":[102],"glucose-1-phosphate,":[103],"fructose-1,6-diphosphate,":[104],"ribose-5-phosphate.":[106],"The":[107,160],"enzymes":[109,137],"more":[111],"active":[112],"to":[113,146,173],"ADP":[114],"ATP":[116],"than":[117],"those":[118],"cytoplasm.":[121],"Mg2+,":[122],"Ni2+,":[123],"NaCN,":[124],"EDTA":[126],"no":[128],"appreciable":[129],"effects":[130],"enzymatic":[133],"activities.":[134],"However,":[135],"strongly":[139],"inhibited":[140],"Hg2+":[142],"Fe3+":[144],"and,":[145],"varied":[147],"degrees,":[148],"Cu2+,":[150],"Zn2+,":[151],"Co2+,":[152],"NaF.":[153],"p-chloromercuribenzoate,":[154],"urea":[156],"that":[158],"order.":[159],"molecular":[161],"weights,":[162],"estimated":[163],"sodium":[165],"dodecylsulfate":[166],"electrophoresis,":[168],"ranged":[169],"28":[171],"000":[172],"64":[174],"000.":[175]},"counts_by_year":[{"year":2022,"cited_by_count":2},{"year":2019,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-09-29T14:09:16.559479","created_date":"2016-06-24T00:00:00"}