{"id":"https://openalex.org/W2166015342","doi":"https://doi.org/10.1515/cclm.1982.20.10.723","title":"Eine empfindliche Methode zur Bestimmung von Glykosaminoglykanverteilungsmustern, angewendet auf 9 Bandscheiben einer menschlichen Wirbels\u00e4ule","display_name":"Eine empfindliche Methode zur Bestimmung von Glykosaminoglykanverteilungsmustern, angewendet auf 9 Bandscheiben einer menschlichen Wirbels\u00e4ule","publication_year":1982,"publication_date":"1982-01-01","ids":{"openalex":"https://openalex.org/W2166015342","doi":"https://doi.org/10.1515/cclm.1982.20.10.723","mag":"2166015342","pmid":"https://pubmed.ncbi.nlm.nih.gov/7142912"},"language":"de","primary_location":{"id":"doi:10.1515/cclm.1982.20.10.723","doi":"https://doi.org/10.1515/cclm.1982.20.10.723","is_oa":true,"landing_page_url":"https://doi.org/10.1515/cclm.1982.20.10.723","pdf_url":"https://edoc.hu-berlin.de/bitstreams/1aa9105c-4b9e-4c0f-a4c8-4a87da19400e/download","source":{"id":"https://openalex.org/S124948411","display_name":"Clinical Chemistry and Laboratory Medicine (CCLM)","issn_l":"1434-6621","issn":["1434-6621","1437-4331"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310313990","host_organization_name":"De Gruyter","host_organization_lineage":["https://openalex.org/P4310313990"],"host_organization_lineage_names":["De Gruyter"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"cclm","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","datacite","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://edoc.hu-berlin.de/bitstreams/1aa9105c-4b9e-4c0f-a4c8-4a87da19400e/download","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5029651572","display_name":"Eberhard Gurr","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I34809795","display_name":"Medizinische Hochschule Hannover","ror":"https://ror.org/00f2yqf98","country_code":"DE","type":"education","lineage":["https://openalex.org/I34809795"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"E. Gurr","raw_affiliation_strings":["Institut f\u00fcr Klinische Chemie II, Medizinische Hochschule Hannover und"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institut f\u00fcr Klinische Chemie II, Medizinische Hochschule Hannover und","institution_ids":["https://openalex.org/I34809795"]}]},{"author_position":"middle","author":{"id":null,"display_name":"R. Schubert","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I34809795","display_name":"Medizinische Hochschule Hannover","ror":"https://ror.org/00f2yqf98","country_code":"DE","type":"education","lineage":["https://openalex.org/I34809795"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"R. Schubert","raw_affiliation_strings":["Institut f\u00fcr Klinische Chemie II, Medizinische Hochschule Hannover und"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institut f\u00fcr Klinische Chemie II, Medizinische Hochschule Hannover und","institution_ids":["https://openalex.org/I34809795"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111673943","display_name":"A. Delbr\u00fcck","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I34809795","display_name":"Medizinische Hochschule Hannover","ror":"https://ror.org/00f2yqf98","country_code":"DE","type":"education","lineage":["https://openalex.org/I34809795"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"A. Delbr\u00fcck","raw_affiliation_strings":["Institut f\u00fcr Klinische Chemie II, Medizinische Hochschule Hannover und"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institut f\u00fcr Klinische Chemie II, Medizinische Hochschule Hannover und","institution_ids":["https://openalex.org/I34809795"]}]},{"author_position":"last","author":{"id":null,"display_name":"W. K\u00f6ller","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I34809795","display_name":"Medizinische Hochschule Hannover","ror":"https://ror.org/00f2yqf98","country_code":"DE","type":"education","lineage":["https://openalex.org/I34809795"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"W. K\u00f6ller","raw_affiliation_strings":["Abteilung f\u00fcr Krankheiten der Bewegungsorgane und des Stoffwechsels, Medizinische Hochschule Hannover"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Abteilung f\u00fcr Krankheiten der Bewegungsorgane und des Stoffwechsels, Medizinische Hochschule Hannover","institution_ids":["https://openalex.org/I34809795"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I34809795"],"apc_list":{"value":2400,"currency":"EUR","value_usd":2712},"apc_paid":null,"fwci":0.0,"has_fulltext":true,"cited_by_count":2,"citation_normalized_percentile":{"value":0.11997226,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"20","issue":"10","first_page":"723","last_page":"730"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11131","display_name":"Proteoglycans and glycosaminoglycans research","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11131","display_name":"Proteoglycans and glycosaminoglycans research","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10238","display_name":"Spine and Intervertebral Disc Pathology","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/2732","display_name":"Orthopedics and Sports Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10105","display_name":"Osteoarthritis Treatment and Mechanisms","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2745","display_name":"Rheumatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/intervertebral-disc","display_name":"intervertebral disc","score":0.9179999828338623},{"id":"https://openalex.org/keywords/anulus-fibrosus","display_name":"anulus fibrosus","score":0.8370000123977661},{"id":"https://openalex.org/keywords/nucleus-pulposus","display_name":"nucleus pulposus","score":0.9990000128746033},{"id":"https://openalex.org/keywords/hyaluronate-lyase","display_name":"hyaluronate lyase","score":0.8970000147819519},{"id":"https://openalex.org/keywords/heparan-sulfate","display_name":"heparan sulfate","score":0.9380000233650208},{"id":"https://openalex.org/keywords/keratan-sulfate","display_name":"keratan sulfate","score":0.9850000143051147},{"id":"https://openalex.org/keywords/spine","display_name":"spine","score":0.5950000286102295}],"concepts":[{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.4788758158683777}],"mesh":[{"descriptor_ui":"D000328","descriptor_name":"Adult","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002808","descriptor_name":"Chondroitin Lyases","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002851","descriptor_name":"Chromatography, High Pressure Liquid","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D003238","descriptor_name":"Connective Tissue","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D006025","descriptor_name":"Glycosaminoglycans","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006868","descriptor_name":"Hydrolysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007403","descriptor_name":"Intervertebral Disc","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011133","descriptor_name":"Polysaccharide-Lyases","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":5,"locations":[{"id":"doi:10.1515/cclm.1982.20.10.723","doi":"https://doi.org/10.1515/cclm.1982.20.10.723","is_oa":true,"landing_page_url":"https://doi.org/10.1515/cclm.1982.20.10.723","pdf_url":"https://edoc.hu-berlin.de/bitstreams/1aa9105c-4b9e-4c0f-a4c8-4a87da19400e/download","source":{"id":"https://openalex.org/S124948411","display_name":"Clinical Chemistry and Laboratory Medicine (CCLM)","issn_l":"1434-6621","issn":["1434-6621","1437-4331"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310313990","host_organization_name":"De Gruyter","host_organization_lineage":["https://openalex.org/P4310313990"],"host_organization_lineage_names":["De Gruyter"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"cclm","raw_type":"journal-article"},{"id":"pmid:7142912","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7142912","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie","raw_type":"English Abstract"},{"id":"pmh:oai:edoc.hu-berlin.de:18452/13015","doi":null,"is_oa":false,"landing_page_url":"https://edoc.hu-berlin.de/18452/13015","pdf_url":null,"source":{"id":"https://openalex.org/S4306400923","display_name":"edoc Publication server (Humboldt University of Berlin)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I39343248","host_organization_name":"Humboldt-Universit\u00e4t zu Berlin","host_organization_lineage":["https://openalex.org/I39343248"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"article"},{"id":"doi:10.18452/12363","doi":"https://doi.org/10.18452/12363","is_oa":true,"landing_page_url":"https://doi.org/10.18452/12363","pdf_url":null,"source":{"id":"https://openalex.org/S4306400923","display_name":"edoc Publication server (Humboldt University of Berlin)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I39343248","host_organization_name":"Humboldt-Universit\u00e4t zu Berlin","host_organization_lineage":["https://openalex.org/I39343248"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null},{"id":"mag:2577704361","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7142912","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":{"id":"doi:10.1515/cclm.1982.20.10.723","doi":"https://doi.org/10.1515/cclm.1982.20.10.723","is_oa":true,"landing_page_url":"https://doi.org/10.1515/cclm.1982.20.10.723","pdf_url":"https://edoc.hu-berlin.de/bitstreams/1aa9105c-4b9e-4c0f-a4c8-4a87da19400e/download","source":{"id":"https://openalex.org/S124948411","display_name":"Clinical Chemistry and Laboratory Medicine (CCLM)","issn_l":"1434-6621","issn":["1434-6621","1437-4331"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310313990","host_organization_name":"De Gruyter","host_organization_lineage":["https://openalex.org/P4310313990"],"host_organization_lineage_names":["De Gruyter"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"cclm","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.4000000059604645,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2166015342.pdf","grobid_xml":"https://content.openalex.org/works/W2166015342.grobid-xml"},"referenced_works_count":15,"referenced_works":["https://openalex.org/W648500737","https://openalex.org/W1518696392","https://openalex.org/W1658991581","https://openalex.org/W1971640461","https://openalex.org/W1971926658","https://openalex.org/W1981647896","https://openalex.org/W1987391355","https://openalex.org/W2057787805","https://openalex.org/W2069389359","https://openalex.org/W2070997284","https://openalex.org/W2092876323","https://openalex.org/W2093531586","https://openalex.org/W2412882951","https://openalex.org/W2413349428","https://openalex.org/W2416828635"],"related_works":["https://openalex.org/W7017910357","https://openalex.org/W250402667","https://openalex.org/W7063980560","https://openalex.org/W2042930022","https://openalex.org/W2058594686","https://openalex.org/W1988523636","https://openalex.org/W2121870454","https://openalex.org/W2082688945","https://openalex.org/W2075649800","https://openalex.org/W7098116036"],"abstract_inverted_index":{"Zusammenfassung:":[0],"Es":[1],"wird":[2],"eine":[3],"Methode":[4,57,82],"zur":[5],"Bestimmung":[6,28],"des":[7,102],"Glykosaminoglykanverteilungsmusters":[8],"in":[9,104,161,297,309,319,324,355],"einem":[10],"isolierten":[11],"Glykosaminoglykanpool":[12],"beschrieben,":[13],"die":[14,34,65,75,349],"auf":[15,83],"der":[16,19,29,37,51,69,81,107,109,114,338,360],"aufeinanderfolgenden":[17],"Anwendung":[18,80],"spezifischen":[20],"Enzyme":[21],"Hyaluronatlyase,":[22],"Chondroitinsulfatlyase":[23],"AC":[24,188],"und":[25,48,92,116,342,358],"ABC":[26],"beruht.Die":[27],"Komponenten":[30],"erfolgt":[31],"empfindlich":[32],"\u00fcber":[33],"Metabolitkonzentrationen":[35],"mithilfe":[36],"Hochleistungsfl\u00fcssigkeitschromatographie":[38],"(Nachweisgrenzen":[39],"unter":[40,353],"0,1":[41],"nmol).Unverdautes":[42],"Heparan-und":[43],"Keratansulfat":[44,341],"kann":[45],"d\u00fcnnschichtchromatographisch":[46],"getrennt":[47],"mit":[49,55,63,67],"Hilfe":[50,68],"Urons\u00e4ure-bzw.Hexosaminbestimm\u00fcng":[52],"quantifiziert":[53],"werden.Die":[54,79],"dieser":[56,329],"ermittelten":[58],"Verteilungsmuster":[59],"stimmen":[60],"nur":[61],"dann":[62],"denen'\u00fcberein,":[64],"man":[66],"lonenaustauschchromatographie":[70],"(Dowex":[71,251],"1X2)":[72],"erh\u00e4lt,":[73],"wenn":[74],"Fraktionen":[76],"enzymatisch":[77],"charakterisiert":[78],"9":[84,143,273],"Bandscheiben":[85],"aus":[86],"einer":[87],"menschlichen":[88],"Wirbels\u00e4ule":[89,115],"(Anulus":[90],"fibrosus":[91,280,322],"Nucleus":[93,124],"pulposus":[94,283],"jeweils":[95],"getrennt)":[96],"ergab":[97],"1)":[98],"keine":[99],"systematische":[100],"Ver\u00e4nderung":[101],"Verteihmgsmusters":[103],"Abh\u00e4ngigkeit":[105],"von":[106,127,345],"Lage":[108],"Zwischenwirbelscheiben":[110],"im":[111,118,121],"mittleren":[112],"Abschnitt":[113],"2)":[117,254,313],"Anulus":[119],"ein":[120],"Vergleich":[122],"zum":[123],"niedrigeres":[125],"Verh\u00e4ltnis":[126],"Galaktosaminoglykan":[128],"zu":[129],"Keratansulfat.A":[130],"sensitive":[131],"method":[132,151,269],"for":[133,155],"the":[134,137,157,176,180,194,228,259,264,286,298,304,307,310,314,320,325],"analysis":[135],"of":[136,193,208,231,263,306],"glycosaminoglycan":[138,158],"distribution":[139,159,238,299],"pattern,":[140],"applied":[141,271],"to":[142,272,294,303],"intervertebral":[144,165],"discs":[145,166,274,308],"ofone":[146],"human":[147,277],"spine":[148,278],"Summary:":[149],"A":[150],"has":[152],"been":[153],"established":[154],"an\u00e4lysing":[156],"pattern":[160],"small":[162],"specimeris":[163],"from":[164,218],"arid":[167],"other":[168,220],"connective":[169],"tissue":[170],"materials.The":[171],"procedure":[172],"is":[173,255],"based":[174],"on":[175],"consecutive":[177],"digestion":[178],"by":[179,199,221,227,241,247],"specific":[181],"enzymes":[182],"hyaluronate":[183],"lyase,":[184],"chondroitin":[185],"sulfate":[186],"lyase":[187],"and":[189,225,234,281,312],"ABC.The":[190],"quantitative":[191],"determination":[192,230],"res\u00fclting":[195],"disaccharides":[196],"was":[197,270,317],"achieved":[198],"high":[200],"performance":[201],"liquid":[202],"chromatography":[203,224,250],"with":[204,244,301],"a":[205],"detection":[206],"limit":[207],"less":[209],"than":[210,323],"0.1":[211],"nmol.The":[212],"undigested":[213],"heparan-and":[214],"keratansulfate":[215],"were":[216,290],"separated":[217],"each":[219],"thin":[222],"layer":[223],"quantified":[226],"eolorimetric":[229],"urpnic":[232],"acid":[233],"hexosamine,":[235],"respectively.Correl\u00e4ti\u00f6n":[236],"\u00f6f":[237,275],"pattenis":[239],"obt\u00e4ined":[240],"this":[242],"jnethod":[243],"those":[245],"obtained":[246],"ion":[248,265],"exchange":[249,266],"l":[252],"X":[253],"not":[256],"ppssible":[257],"without":[258],"additiqnal":[260],"enzymatic":[261],"characterization":[262],"Chromatographie":[267],"fractions.The":[268],"one":[276],"(anulus":[279],"nucleus":[282,326],"separately)":[284],".with":[285],"f\u00f6liowirig":[287],"results:1)":[288],"there":[289],"no":[291],"systematic":[292],"changes":[293],"be":[295],"seen":[296],"p\u00e4tterns":[300],"respect":[302],"location":[305],"spine,":[311],"galactosaminoglycan/keratansulfate":[315],"ratio":[316],"higher":[318],"anulus":[321],"pulposus.":[327],"Einf\u00fchrungVertreter":[328],"Stoffklasse":[330],"(Chondroitin-4-und":[331],"-6-sulfat,":[332],"Die":[333],"Glykosaminoglykane":[334],"sind":[335],"als":[336],"Bestandteile":[337],"extra-Heparansulfat,":[339],"Dermatansulfat,":[340],"Hyaluzellul\u00e4ren":[343],"Matrix":[344],"wesentlicher":[346],"Bedeutung":[347],"f\u00fcr":[348],"ronat)":[350],"unterscheiden":[351],"sich":[352],"anderem":[354],"ihrem":[356],"physi-Struktur":[357],"Funktion":[359],"Bindegewebe.Die":[361],"einzelnen":[362],"kaiisch-chemischen":[363],"Verhalten.Zu":[364],"ihrer":[365],"quantitativen":[366]},"counts_by_year":[],"updated_date":"2026-11-08T12:12:28.789561","created_date":"2016-06-24T00:00:00"}