{"id":"https://openalex.org/W2165928362","doi":"https://doi.org/10.1046/j.1365-2958.1996.01521.x","title":"Molecular cloning and functional analysis of a novel macrolide\u2010resistance determinant, mefA, from Streptococcus pyogenes","display_name":"Molecular cloning and functional analysis of a novel macrolide\u2010resistance determinant, mefA, from Streptococcus pyogenes","publication_year":1996,"publication_date":"1996-12-01","ids":{"openalex":"https://openalex.org/W2165928362","doi":"https://doi.org/10.1046/j.1365-2958.1996.01521.x","mag":"2165928362","pmid":"https://pubmed.ncbi.nlm.nih.gov/8971709"},"language":"en","primary_location":{"id":"doi:10.1046/j.1365-2958.1996.01521.x","doi":"https://doi.org/10.1046/j.1365-2958.1996.01521.x","is_oa":false,"landing_page_url":"https://doi.org/10.1046/j.1365-2958.1996.01521.x","pdf_url":null,"source":{"id":"https://openalex.org/S102549058","display_name":"Molecular Microbiology","issn_l":"0950-382X","issn":["0950-382X","1365-2958"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular Microbiology","raw_type":"journal-article"},"parent_works":[],"lineage":["https://openalex.org/W2165928362"],"child_works_count":0,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5103593832","display_name":"Joanna Clancy","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I180857899","display_name":"Pfizer (United States)","ror":"https://ror.org/01xdqrp08","country_code":"US","type":"company","lineage":["https://openalex.org/I180857899"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Joanna Clancy","raw_affiliation_strings":["Central Research Division, Pfizer Incorporated, Groton, Connecticut 06340, USA. joannavclancy@groton.pfizer.com","Central Research Division, Pfizer Incorporated, Groton, Connecticut 06340, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Central Research Division, Pfizer Incorporated, Groton, Connecticut 06340, USA. joannavclancy@groton.pfizer.com","institution_ids":["https://openalex.org/I180857899"]},{"raw_affiliation_string":"Central Research Division, Pfizer Incorporated, Groton, Connecticut 06340, USA","institution_ids":["https://openalex.org/I180857899"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5032487565","display_name":"J.W. Petitpas","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I180857899","display_name":"Pfizer (United States)","ror":"https://ror.org/01xdqrp08","country_code":"US","type":"company","lineage":["https://openalex.org/I180857899"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Joan Petitpas","raw_affiliation_strings":["Central Research Division, Pfizer Incorporated, Groton, Connecticut 06340, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Central Research Division, Pfizer Incorporated, Groton, Connecticut 06340, USA","institution_ids":["https://openalex.org/I180857899"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5102830503","display_name":"Fadia B. Dib-Hajj","orcid":"https://orcid.org/0000-0002-5026-1351","observed_orcids":["https://orcid.org/0000-0002-5026-1351"]},"institutions":[{"id":"https://openalex.org/I180857899","display_name":"Pfizer (United States)","ror":"https://ror.org/01xdqrp08","country_code":"US","type":"company","lineage":["https://openalex.org/I180857899"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Fadia Dib\u2010Hajj","raw_affiliation_strings":["Central Research Division, Pfizer Incorporated, Groton, Connecticut 06340, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Central Research Division, Pfizer Incorporated, Groton, Connecticut 06340, USA","institution_ids":["https://openalex.org/I180857899"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101631196","display_name":"Wei Yuan","orcid":"https://orcid.org/0009-0001-3427-6764","observed_orcids":["https://orcid.org/0009-0001-3427-6764","https://orcid.org/0009-0009-3395-8366"]},"institutions":[{"id":"https://openalex.org/I180857899","display_name":"Pfizer (United States)","ror":"https://ror.org/01xdqrp08","country_code":"US","type":"company","lineage":["https://openalex.org/I180857899"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Wei Yuan","raw_affiliation_strings":["Central Research Division, Pfizer Incorporated, Groton, Connecticut 06340, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Central Research Division, Pfizer Incorporated, Groton, Connecticut 06340, USA","institution_ids":["https://openalex.org/I180857899"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5040832420","display_name":"Melissa T. Cronan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I180857899","display_name":"Pfizer (United States)","ror":"https://ror.org/01xdqrp08","country_code":"US","type":"company","lineage":["https://openalex.org/I180857899"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Melissa Cronan","raw_affiliation_strings":["Central Research Division, Pfizer Incorporated, Groton, Connecticut 06340, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Central Research Division, Pfizer Incorporated, Groton, Connecticut 06340, USA","institution_ids":["https://openalex.org/I180857899"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109038444","display_name":"Ajith V. Kamath","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I180857899","display_name":"Pfizer (United States)","ror":"https://ror.org/01xdqrp08","country_code":"US","type":"company","lineage":["https://openalex.org/I180857899"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ajith V. Kamath","raw_affiliation_strings":["Central Research Division, Pfizer Incorporated, Groton, Connecticut 06340, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Central Research Division, Pfizer Incorporated, Groton, Connecticut 06340, USA","institution_ids":["https://openalex.org/I180857899"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5076225399","display_name":"JAY M. BERGERON","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I180857899","display_name":"Pfizer (United States)","ror":"https://ror.org/01xdqrp08","country_code":"US","type":"company","lineage":["https://openalex.org/I180857899"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jay Bergeron","raw_affiliation_strings":["Central Research Division, Pfizer Incorporated, Groton, Connecticut 06340, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Central Research Division, Pfizer Incorporated, Groton, Connecticut 06340, USA","institution_ids":["https://openalex.org/I180857899"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5111551934","display_name":"James A. Retsema","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I180857899","display_name":"Pfizer (United States)","ror":"https://ror.org/01xdqrp08","country_code":"US","type":"company","lineage":["https://openalex.org/I180857899"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"James A. Retsema","raw_affiliation_strings":["Central Research Division, Pfizer Incorporated, Groton, Connecticut 06340, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Central Research Division, Pfizer Incorporated, Groton, Connecticut 06340, USA","institution_ids":["https://openalex.org/I180857899"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5103593832"],"corresponding_institution_ids":["https://openalex.org/I180857899"],"apc_list":{"value":4430,"currency":"USD","value_usd":4430},"apc_paid":null,"fwci":11.3849,"has_fulltext":false,"cited_by_count":366,"citation_normalized_percentile":{"value":0.99257885,"is_in_top_1_percent":true,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":99},"biblio":{"volume":"22","issue":"5","first_page":"867","last_page":"879"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10147","display_name":"Antibiotic Resistance in Bacteria","score":0.8744999766349792,"subfield":{"id":"https://openalex.org/subfields/2726","display_name":"Microbiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10147","display_name":"Antibiotic Resistance in Bacteria","score":0.8744999766349792,"subfield":{"id":"https://openalex.org/subfields/2726","display_name":"Microbiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10195","display_name":"Antimicrobial Resistance in Staphylococcus","score":0.06849999725818634,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11153","display_name":"Streptococcal Infections and Treatments","score":0.021299999207258224,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/mefa","display_name":"mefA","score":0.7689999938011169},{"id":"https://openalex.org/keywords/macrolide-resistance","display_name":"macrolide resistance","score":0.9139999747276306},{"id":"https://openalex.org/keywords/streptococcus-pyogenes","display_name":"Streptococcus pyogenes","score":0.9959999918937683},{"id":"https://openalex.org/keywords/antiporters","display_name":"antiporters","score":0.5860000252723694},{"id":"https://openalex.org/keywords/gene-cloning","display_name":"gene cloning","score":0.5979999899864197},{"id":"https://openalex.org/keywords/heterologous-expression","display_name":"heterologous expression","score":0.6150000095367432},{"id":"https://openalex.org/keywords/intracellular-drug-accumulation","display_name":"intracellular drug accumulation","score":0.7699999809265137},{"id":"https://openalex.org/keywords/lincosamide-resistance","display_name":"lincosamide resistance","score":0.6639999747276306}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8986079096794128},{"id":"https://openalex.org/C2779306812","wikidata":"https://www.wikidata.org/wiki/Q131271","display_name":"Streptococcus pyogenes","level":4,"score":0.7304444313049316},{"id":"https://openalex.org/C2780685696","wikidata":"https://www.wikidata.org/wiki/Q422235","display_name":"Lincosamides","level":4,"score":0.6871118545532227},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.6006948947906494},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.5564916133880615},{"id":"https://openalex.org/C200082930","wikidata":"https://www.wikidata.org/wiki/Q22327148","display_name":"Efflux","level":2,"score":0.43884485960006714},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.43018195033073425},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.4284825921058655},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.42383551597595215},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.396372526884079},{"id":"https://openalex.org/C2780206985","wikidata":"https://www.wikidata.org/wiki/Q213511","display_name":"Erythromycin","level":3,"score":0.2549308240413666},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.1409098505973816},{"id":"https://openalex.org/C501593827","wikidata":"https://www.wikidata.org/wiki/Q12187","display_name":"Antibiotics","level":2,"score":0.1196742057800293},{"id":"https://openalex.org/C2779489039","wikidata":"https://www.wikidata.org/wiki/Q188121","display_name":"Staphylococcus aureus","level":3,"score":0.0737999975681305}],"mesh":[{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004269","descriptor_name":"DNA, Bacterial","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004352","descriptor_name":"Drug Resistance, Microbial","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D004917","descriptor_name":"Erythromycin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004917","descriptor_name":"Erythromycin","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D005798","descriptor_name":"Genes, Bacterial","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008565","descriptor_name":"Membrane Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D008565","descriptor_name":"Membrane Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012270","descriptor_name":"Ribosomes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013297","descriptor_name":"Streptococcus pyogenes","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D013698","descriptor_name":"Templates, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016296","descriptor_name":"Mutagenesis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1046/j.1365-2958.1996.01521.x","doi":"https://doi.org/10.1046/j.1365-2958.1996.01521.x","is_oa":false,"landing_page_url":"https://doi.org/10.1046/j.1365-2958.1996.01521.x","pdf_url":null,"source":{"id":"https://openalex.org/S102549058","display_name":"Molecular Microbiology","issn_l":"0950-382X","issn":["0950-382X","1365-2958"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular Microbiology","raw_type":"journal-article"},{"id":"pmid:8971709","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8971709","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular microbiology","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.5683000087738037}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2153934798","https://openalex.org/W2097299359","https://openalex.org/W2091597966","https://openalex.org/W2786875625","https://openalex.org/W2132507709","https://openalex.org/W2127686385","https://openalex.org/W2105345749","https://openalex.org/W2105523198","https://openalex.org/W3024782954"],"abstract_inverted_index":{"Several":[0],"streptococcal":[1],"strains":[2,167],"had":[3,15],"an":[4],"uncharacterized":[5],"mechanism":[6,24,160],"of":[7,40,57,82,91,144,161,175,195,230],"macrolide":[8,154],"resistance":[9,26],"that":[10,14,105,207,218],"differed":[11],"from":[12,53,130],"those":[13],"been":[16,150],"reported":[17],"previously":[18],"in":[19,74,100,138,163,197,209,240],"the":[20,68,83,101,106,131,136,145,169,184,198,219,231,250],"literature.":[21],"This":[22,212],"novel":[23,110,223],"conveyed":[25],"to":[27,34,114,183,236],"14-":[28],"and":[29,72,94,128],"15-membered":[30],"macrolides,":[31,36],"but":[32],"not":[33],"16-membered":[35],"lincosamides":[37],"or":[38],"analogues":[39],"streptogramin":[41],"B.":[42],"The":[43,80,118,233],"gene":[44,107,220,234],"encoding":[45],"this":[46,179],"phenotype":[47],"was":[48,86,122,203],"cloned":[49,146,170],"by":[50,88,246],"standard":[51],"methods":[52],"total":[54],"genomic":[55],"digests":[56],"Streptococcus":[58],"pyogenes":[59,201,242],"02C1064":[60],"as":[61,244],"a":[62,109,125,216,222],"4.7":[63],"kb":[64],"heterologous":[65],"insert":[66],"into":[67,124],"low-copy":[69],"vector,":[70],"pACYC177,":[71],"expressed":[73,129],"several":[75],"Escherichia":[76],"coli":[77,140],"K-12":[78],"strains.":[79,211],"location":[81],"macrolide-resistance":[84,119],"determinant":[85,171],"established":[87],"functional":[89],"analysis":[90],"deletion":[92],"derivatives":[93],"sequencing.":[95],"A":[96],"search":[97],"for":[98,153,158],"homologues":[99],"genetic":[102],"databases":[103],"confirmed":[104],"is":[108,181,213],"one":[111],"with":[112,215],"homology":[113],"membrane-associated":[115],"pump":[116],"proteins.":[117],"coding":[120],"sequence":[121],"subcloned":[123],"pET23a":[126],"vector":[127],"inducible":[132],"T7":[133],"promoter":[134],"on":[135],"plasmid":[137],"E.":[139],"BL21(DE3).":[141],"Physiological":[142],"studies":[143],"determinant,":[147],"which":[148,226],"has":[149],"named":[151],"mefA":[152],"efflux,":[155],"provide":[156],"evidence":[157],"its":[159],"action":[162],"host":[164],"bacteria.":[165],"E.coli":[166],"containing":[168],"maintain":[172],"lower":[173,205],"levels":[174],"intracellular":[176,193],"erythromycin":[177,228],"when":[178],"compound":[180],"added":[182],"external":[185],"medium":[186],"than":[187,206],"isogenic":[188],"clones":[189],"without":[190],"mefA.":[191],"Furthermore,":[192],"accumulation":[194],"[14C]-erythromycin":[196],"original":[199],"S.":[200,241],"strain":[202],"always":[204],"observed":[208],"erythromycin-sensitive":[210],"consistent":[214],"hypothesis":[217],"encodes":[221],"antiporter":[224],"function":[225],"pumps":[227],"out":[229],"cell.":[232],"appears":[235],"be":[237],"widely":[238],"distributed":[239],"strains,":[243],"demonstrated":[245],"primer-specific":[247],"synthesis":[248],"using":[249],"polymerase":[251],"chain":[252],"reaction.":[253]},"counts_by_year":[{"year":2026,"cited_by_count":5},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":7},{"year":2023,"cited_by_count":5},{"year":2022,"cited_by_count":6},{"year":2021,"cited_by_count":11},{"year":2020,"cited_by_count":8},{"year":2019,"cited_by_count":5},{"year":2018,"cited_by_count":9},{"year":2017,"cited_by_count":7},{"year":2016,"cited_by_count":8},{"year":2015,"cited_by_count":13},{"year":2014,"cited_by_count":11},{"year":2013,"cited_by_count":5},{"year":2012,"cited_by_count":5}],"updated_date":"2026-11-14T11:04:58.165337","created_date":"2016-06-24T00:00:00"}