{"id":"https://openalex.org/W2165339473","doi":"https://doi.org/10.1172/jci109018","title":"Attachment role of gonococcal pili. Optimum conditions and quantitation of adherence of isolated pili to human cells in vitro.","display_name":"Attachment role of gonococcal pili. Optimum conditions and quantitation of adherence of isolated pili to human cells in vitro.","publication_year":1978,"publication_date":"1978-04-01","ids":{"openalex":"https://openalex.org/W2165339473","doi":"https://doi.org/10.1172/jci109018","mag":"2165339473","pmid":"https://pubmed.ncbi.nlm.nih.gov/96134","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/372611"},"language":"en","primary_location":{"id":"doi:10.1172/jci109018","doi":"https://doi.org/10.1172/jci109018","is_oa":true,"landing_page_url":"https://doi.org/10.1172/jci109018","pdf_url":"http://www.jci.org/articles/view/109018/files/pdf","source":{"id":"https://openalex.org/S114430552","display_name":"Journal of Clinical Investigation","issn_l":"0021-9738","issn":["0021-9738","1558-8238"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320279","host_organization_name":"American Society for Clinical Investigation","host_organization_lineage":["https://openalex.org/P4310320279"],"host_organization_lineage_names":["American Society for Clinical Investigation"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Clinical Investigation","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"http://www.jci.org/articles/view/109018/files/pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"W A Pearce","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"W A Pearce","raw_affiliation_strings":["Departments of Pathobiology and Medicine, University of Washington, and the Immunology Research Laboratory, U. S. Public Health Service Hospital, Seattle, Washington 98114","Master of Science in Public Health, Department of Pathobiology, University of Washington"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Pathobiology and Medicine, University of Washington, and the Immunology Research Laboratory, U. S. Public Health Service Hospital, Seattle, Washington 98114","institution_ids":["https://openalex.org/I201448701"]},{"raw_affiliation_string":"Master of Science in Public Health, Department of Pathobiology, University of Washington","institution_ids":["https://openalex.org/I201448701"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5111478229","display_name":"Thomas M. Buchanan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"T M Buchanan","raw_affiliation_strings":["Departments of Pathobiology and Medicine, University of Washington, and the Immunology Research Laboratory, U. S. Public Health Service Hospital, Seattle, Washington 98114"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Pathobiology and Medicine, University of Washington, and the Immunology Research Laboratory, U. S. Public Health Service Hospital, Seattle, Washington 98114","institution_ids":["https://openalex.org/I201448701"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I201448701"],"apc_list":{"value":5200,"currency":"USD","value_usd":5200},"apc_paid":null,"fwci":13.1526,"has_fulltext":true,"cited_by_count":96,"citation_normalized_percentile":{"value":0.99468085,"is_in_top_1_percent":true,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"61","issue":"4","first_page":"931","last_page":"943"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10873","display_name":"Bacterial Infections and Vaccines","score":0.5292999744415283,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10873","display_name":"Bacterial Infections and Vaccines","score":0.5292999744415283,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10428","display_name":"Reproductive tract infections research","score":0.3093000054359436,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13326","display_name":"Biochemical and Structural Characterization","score":0.11140000075101852,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/neisseria-gonorrhoeae","display_name":"Neisseria gonorrhoeae","score":0.9279999732971191},{"id":"https://openalex.org/keywords/bacterial-adhesion","display_name":"bacterial adhesion","score":0.7559999823570251},{"id":"https://openalex.org/keywords/host-cell-attachment","display_name":"host cell attachment","score":0.6610000133514404},{"id":"https://openalex.org/keywords/buccal-cells","display_name":"buccal epithelial cells","score":0.9190000295639038},{"id":"https://openalex.org/keywords/fallopian-tube-mucosa","display_name":"fallopian tube mucosa","score":0.8769999742507935}],"concepts":[{"id":"https://openalex.org/C174832134","wikidata":"https://www.wikidata.org/wiki/Q934786","display_name":"Pilus","level":4,"score":0.9866578578948975},{"id":"https://openalex.org/C2779166590","wikidata":"https://www.wikidata.org/wiki/Q131129","display_name":"Neisseria gonorrhoeae","level":2,"score":0.7259494662284851},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.6011134386062622},{"id":"https://openalex.org/C2910768159","wikidata":"https://www.wikidata.org/wiki/Q397875","display_name":"Fimbriae Proteins","level":5,"score":0.5703310966491699},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.5511783361434937},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5497036576271057},{"id":"https://openalex.org/C39103817","wikidata":"https://www.wikidata.org/wiki/Q1810775","display_name":"Fimbria","level":4,"score":0.5228883624076843},{"id":"https://openalex.org/C28005876","wikidata":"https://www.wikidata.org/wiki/Q189814","display_name":"Endocytosis","level":3,"score":0.49088746309280396},{"id":"https://openalex.org/C179470777","wikidata":"https://www.wikidata.org/wiki/Q356661","display_name":"Bacterial adhesin","level":4,"score":0.47642382979393005},{"id":"https://openalex.org/C2780115692","wikidata":"https://www.wikidata.org/wiki/Q5747302","display_name":"Heterologous","level":3,"score":0.45611685514450073},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.34344303607940674},{"id":"https://openalex.org/C60987743","wikidata":"https://www.wikidata.org/wiki/Q1460232","display_name":"Virulence","level":3,"score":0.3183448314666748},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.211513489484787},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.06970536708831787}],"mesh":[{"descriptor_ui":"D000907","descriptor_name":"Antibodies, Bacterial","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002584","descriptor_name":"Cervix Uteri","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":false},{"descriptor_ui":"D004912","descriptor_name":"Erythrocytes","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":false},{"descriptor_ui":"D005187","descriptor_name":"Fallopian Tubes","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":false},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006367","descriptor_name":"HeLa Cells","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009061","descriptor_name":"Mouth Mucosa","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":false},{"descriptor_ui":"D009344","descriptor_name":"Neisseria gonorrhoeae","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D009344","descriptor_name":"Neisseria gonorrhoeae","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false},{"descriptor_ui":"D009504","descriptor_name":"Neutrophils","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":false},{"descriptor_ui":"D014066","descriptor_name":"Palatine Tonsil","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":false},{"descriptor_ui":"D014621","descriptor_name":"Vagina","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1172/jci109018","doi":"https://doi.org/10.1172/jci109018","is_oa":true,"landing_page_url":"https://doi.org/10.1172/jci109018","pdf_url":"http://www.jci.org/articles/view/109018/files/pdf","source":{"id":"https://openalex.org/S114430552","display_name":"Journal of Clinical Investigation","issn_l":"0021-9738","issn":["0021-9738","1558-8238"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320279","host_organization_name":"American Society for Clinical Investigation","host_organization_lineage":["https://openalex.org/P4310320279"],"host_organization_lineage_names":["American Society for Clinical Investigation"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Clinical Investigation","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:372611","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/372611","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Clin Invest","raw_type":"Text"},{"id":"pmid:96134","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/96134","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of clinical investigation","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1172/jci109018","doi":"https://doi.org/10.1172/jci109018","is_oa":true,"landing_page_url":"https://doi.org/10.1172/jci109018","pdf_url":"http://www.jci.org/articles/view/109018/files/pdf","source":{"id":"https://openalex.org/S114430552","display_name":"Journal of Clinical Investigation","issn_l":"0021-9738","issn":["0021-9738","1558-8238"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320279","host_organization_name":"American Society for Clinical Investigation","host_organization_lineage":["https://openalex.org/P4310320279"],"host_organization_lineage_names":["American Society for Clinical Investigation"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Clinical Investigation","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.8048999905586243}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2165339473.pdf","grobid_xml":"https://content.openalex.org/works/W2165339473.grobid-xml"},"referenced_works_count":55,"referenced_works":["https://openalex.org/W64985841","https://openalex.org/W79381424","https://openalex.org/W258376682","https://openalex.org/W309304698","https://openalex.org/W605229633","https://openalex.org/W1480967651","https://openalex.org/W1491185682","https://openalex.org/W1495169618","https://openalex.org/W1524005751","https://openalex.org/W1525926169","https://openalex.org/W1532436604","https://openalex.org/W1552439704","https://openalex.org/W1558808471","https://openalex.org/W1669384271","https://openalex.org/W1775749144","https://openalex.org/W1820746812","https://openalex.org/W1840917369","https://openalex.org/W1893651774","https://openalex.org/W1895452301","https://openalex.org/W1899753260","https://openalex.org/W1905518185","https://openalex.org/W1909306713","https://openalex.org/W1935010865","https://openalex.org/W1942110032","https://openalex.org/W1946990273","https://openalex.org/W1959705445","https://openalex.org/W1979052367","https://openalex.org/W1982076963","https://openalex.org/W1988714926","https://openalex.org/W1990986783","https://openalex.org/W1991655565","https://openalex.org/W1995202541","https://openalex.org/W2002556221","https://openalex.org/W2013295182","https://openalex.org/W2018628705","https://openalex.org/W2032095782","https://openalex.org/W2045947421","https://openalex.org/W2058552152","https://openalex.org/W2071471566","https://openalex.org/W2074546937","https://openalex.org/W2077853217","https://openalex.org/W2081827000","https://openalex.org/W2113200077","https://openalex.org/W2114473625","https://openalex.org/W2116057909","https://openalex.org/W2124664358","https://openalex.org/W2159401383","https://openalex.org/W2164921778","https://openalex.org/W2166825483","https://openalex.org/W2414122522","https://openalex.org/W2418097994","https://openalex.org/W2468599689","https://openalex.org/W2741110463","https://openalex.org/W4229712605","https://openalex.org/W6677055761"],"related_works":["https://openalex.org/W1568307430","https://openalex.org/W2107316634","https://openalex.org/W1820746812","https://openalex.org/W6982050982","https://openalex.org/W2006832058","https://openalex.org/W7025008605","https://openalex.org/W2017297182","https://openalex.org/W2094048251","https://openalex.org/W2069868445","https://openalex.org/W7097650115"],"abstract_inverted_index":{"Gonoccocal":[0],"pili":[1,18,26,38,58,90,106,144,193,195,227,234,242],"facilitate":[2],"attachment":[3,14,36,83,134,214,235],"of":[4,37,64,82,88,103,120,136,142,152,203,212,221,233,243],"virulent":[5],"Neisseria":[6],"gonorrhoeae":[7],"to":[8,33,39,98,126,145,192,210,241],"human":[9,41,146],"cells.":[10],"To":[11],"characterize":[12],"this":[13],"function,":[15],"purified":[16],"gonococcal":[17,207],"isolated":[19],"from":[20,100],"four":[21],"strains":[22],"possessing":[23],"antigenically":[24],"distinct":[25],"were":[27,109],"radiolabeled":[28],"with":[29,150,239],"125I":[30],"and":[31,47,55,174,228],"used":[32],"measure":[34],"the":[35,133,222,229,244],"various":[40],"cells":[42,94,99,147],"in":[43,59],"vitro.":[44],"Human":[45],"buccal":[46],"cervical-vaginal":[48,116],"mucosal":[49],"epithealial":[50],"cells,":[51,74],"fallopian":[52],"tube":[53],"mucosa,":[54],"sperm":[56],"bound":[57],"greater":[60,86,165,169,197],"numbers":[61],"per":[62,115],"micrometer2":[63],"surface":[65],"area":[66],"(1--10)":[67],"than":[68,166,170,179,182,185,198],"fetal":[69],"tonsil":[70],"fibroblasts,":[71],"HeLa":[72],"M":[73],"erythrocytes,":[75],"or":[76,96],"polymorphonuclear":[77],"leukocytes.":[78],"This":[79],"cell":[80,129],"specificity":[81],"suggests":[84],"a":[85,127,219],"density":[87],"membrane":[89],"binding":[91,107],"sites":[92,102,108,155],"on":[93],"similar":[95],"identical":[97],"natural":[101],"infection.":[104],"The":[105,201],"quantitated":[110],"as":[111,218],"1":[112],"X":[113],"10(4)":[114],"squamous":[117],"cell.":[118],"Pili":[119],"all":[121],"antigenic":[122,224],"types":[123],"attached":[124],"equally":[125],"given":[128],"type,":[130],"implying":[131],"that":[132,231],"moiety":[135],"each":[137],"pilus":[138],"was":[139,160,188],"similar.":[140],"Attachement":[141],"gonoccocal":[143],"occurred":[148],"quickly":[149],"saturation":[151],"presumed":[153],"receptor":[154],"within":[156],"20--60":[157],"min.":[158],"Attachment":[159,187],"temperature":[161],"dependent":[162,176],"(37":[163],"degrees":[164,168,172],"20":[167],"4":[171],"C),":[173],"pH":[175],"(3.5":[177],"less":[178,181,184],"4.5":[180],"5.5":[183],"7.5).":[186],"inhibited":[189],"by":[190],"antibody":[191,240],"(homologous":[194],"Ab":[196],"heterologous":[199],"Ab).":[200],"extent":[202],"possible":[204],"protection":[205],"against":[206],"infection":[208],"due":[209],"inhibition":[211],"pili-mediated":[213],"might":[215],"prove":[216],"limited":[217],"result":[220],"considerable":[223],"heterogeneity":[225],"among":[226],"observation":[230],"blockage":[232],"is":[236],"maximal":[237],"only":[238],"infecting":[245],"strain.":[246]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":2},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}