{"id":"https://openalex.org/W2164508239","doi":"https://doi.org/10.1002/0471142301.ns0526s39","title":"Lentiviral Strategies for RNAi Knockdown of Neuronal Genes","display_name":"Lentiviral Strategies for RNAi Knockdown of Neuronal Genes","publication_year":2007,"publication_date":"2007-04-01","ids":{"openalex":"https://openalex.org/W2164508239","doi":"https://doi.org/10.1002/0471142301.ns0526s39","mag":"2164508239","pmid":"https://pubmed.ncbi.nlm.nih.gov/18428658"},"language":"en","primary_location":{"id":"doi:10.1002/0471142301.ns0526s39","doi":"https://doi.org/10.1002/0471142301.ns0526s39","is_oa":false,"landing_page_url":"https://doi.org/10.1002/0471142301.ns0526s39","pdf_url":null,"source":{"id":"https://openalex.org/S4210168458","display_name":"Current Protocols in Neuroscience","issn_l":"1934-8576","issn":["1934-8576","1934-8584"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","poland-70"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Current Protocols in Neuroscience","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5074225272","display_name":"Jill R. Crittenden","orcid":"https://orcid.org/0000-0002-1981-1917","observed_orcids":["https://orcid.org/0000-0002-1981-1917"]},"institutions":[{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jill R. Crittenden","raw_affiliation_strings":["Massachusetts Institute of Technology Cambridge Massachusetts"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Massachusetts Institute of Technology Cambridge Massachusetts","institution_ids":["https://openalex.org/I63966007"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5004485524","display_name":"Amy J. Heidersbach","orcid":"https://orcid.org/0000-0002-5601-2073","observed_orcids":["https://orcid.org/0000-0002-5601-2073"]},"institutions":[{"id":"https://openalex.org/I180670191","display_name":"University of California, San Francisco","ror":"https://ror.org/043mz5j54","country_code":"US","type":"education","lineage":["https://openalex.org/I180670191"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Amy Heidersbach","raw_affiliation_strings":["University of California, San Francisco San Francisco California"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of California, San Francisco San Francisco California","institution_ids":["https://openalex.org/I180670191"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5039780162","display_name":"Michael T. McManus","orcid":"https://orcid.org/0000-0003-3013-6569","observed_orcids":["https://orcid.org/0000-0003-3013-6569"]},"institutions":[{"id":"https://openalex.org/I180670191","display_name":"University of California, San Francisco","ror":"https://ror.org/043mz5j54","country_code":"US","type":"education","lineage":["https://openalex.org/I180670191"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Michael T. McManus","raw_affiliation_strings":["University of California, San Francisco San Francisco California"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of California, San Francisco San Francisco California","institution_ids":["https://openalex.org/I180670191"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":5050,"currency":"USD","value_usd":5050},"apc_paid":null,"fwci":0.6167,"has_fulltext":false,"cited_by_count":11,"citation_normalized_percentile":{"value":0.64784642,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":95},"biblio":{"volume":"39","issue":"1","first_page":"Unit 5.26","last_page":"Unit 5.26"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.6211000084877014,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.6211000084877014,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.3765000104904175,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.00039999998989515007,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/rna-interference","display_name":"RNA interference","score":0.8309999704360962},{"id":"https://openalex.org/keywords/sirna-knockdown","display_name":"siRNA knockdown","score":0.7649999856948853},{"id":"https://openalex.org/keywords/lentiviral-vectors","display_name":"lentiviral vectors","score":0.9539999961853027},{"id":"https://openalex.org/keywords/neuronal-gene-expression","display_name":"neuronal gene expression","score":0.875},{"id":"https://openalex.org/keywords/post-transcriptional-gene-silencing","display_name":"post-transcriptional gene silencing","score":0.8519999980926514}],"concepts":[{"id":"https://openalex.org/C173396325","wikidata":"https://www.wikidata.org/wiki/Q1501275","display_name":"Gene knockdown","level":3,"score":0.9514840841293335},{"id":"https://openalex.org/C166703698","wikidata":"https://www.wikidata.org/wiki/Q201993","display_name":"RNA interference","level":4,"score":0.9026476144790649},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.6006649732589722},{"id":"https://openalex.org/C32022120","wikidata":"https://www.wikidata.org/wiki/Q797225","display_name":"Interference (communication)","level":3,"score":0.4690326452255249},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.450437068939209},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.36242759227752686},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3275391161441803},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.14803928136825562},{"id":"https://openalex.org/C31258907","wikidata":"https://www.wikidata.org/wiki/Q1301371","display_name":"Computer network","level":1,"score":0.11242038011550903},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.09814026951789856},{"id":"https://openalex.org/C127162648","wikidata":"https://www.wikidata.org/wiki/Q16858953","display_name":"Channel (broadcasting)","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008967","descriptor_name":"Molecular Biology","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D009474","descriptor_name":"Neurons","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D016086","descriptor_name":"Lentivirus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D034622","descriptor_name":"RNA Interference","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1002/0471142301.ns0526s39","doi":"https://doi.org/10.1002/0471142301.ns0526s39","is_oa":false,"landing_page_url":"https://doi.org/10.1002/0471142301.ns0526s39","pdf_url":null,"source":{"id":"https://openalex.org/S4210168458","display_name":"Current Protocols in Neuroscience","issn_l":"1934-8576","issn":["1934-8576","1934-8584"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","poland-70"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Current Protocols in Neuroscience","raw_type":"journal-article"},{"id":"pmid:18428658","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/18428658","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Current protocols in neuroscience","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.5683000087738037,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":34,"referenced_works":["https://openalex.org/W1566969252","https://openalex.org/W1769569140","https://openalex.org/W1931443173","https://openalex.org/W1963143659","https://openalex.org/W1964826832","https://openalex.org/W1967633197","https://openalex.org/W1974861468","https://openalex.org/W1988967884","https://openalex.org/W1993501781","https://openalex.org/W1995004137","https://openalex.org/W1999596958","https://openalex.org/W2002615250","https://openalex.org/W2017642141","https://openalex.org/W2038783205","https://openalex.org/W2047028501","https://openalex.org/W2050912412","https://openalex.org/W2071596792","https://openalex.org/W2072543946","https://openalex.org/W2075278057","https://openalex.org/W2079945542","https://openalex.org/W2082213106","https://openalex.org/W2084828626","https://openalex.org/W2089970931","https://openalex.org/W2101722758","https://openalex.org/W2103533569","https://openalex.org/W2107235611","https://openalex.org/W2135151730","https://openalex.org/W2141886408","https://openalex.org/W2149485430","https://openalex.org/W2153747430","https://openalex.org/W2166802510","https://openalex.org/W2167523976","https://openalex.org/W2171757104","https://openalex.org/W4206226876"],"related_works":["https://openalex.org/W2411576029","https://openalex.org/W1586418235","https://openalex.org/W2168353195","https://openalex.org/W2032396869","https://openalex.org/W4312660805","https://openalex.org/W2187621684","https://openalex.org/W1522944545","https://openalex.org/W2328190627","https://openalex.org/W2085538564","https://openalex.org/W2116409606"],"abstract_inverted_index":{"RNA":[0],"interference":[1],"(RNAi)":[2],"refers":[3],"to":[4,10,46,88,111],"the":[5,35,49,72,75,127],"process":[6,24],"by":[7,28,55,68,86],"which":[8,87],"21-":[9],"23-nucleotide":[11],"short":[12],"interfering":[13],"RNAs":[14],"(siRNAs)":[15],"mediate":[16],"post-transcriptional":[17],"degradation":[18],"of":[19,37,51,71,126],"homologous":[20],"mRNA":[21],"transcripts.":[22],"This":[23,107],"is":[25],"carried":[26],"out":[27],"an":[29],"endogenous":[30],"pathway":[31],"that":[32],"centers":[33],"on":[34],"use":[36],"endogenously":[38],"encoded":[39],"small":[40],"RNAs,":[41],"and":[42,113,124,143],"can":[43,65,94],"be":[44,66],"hijacked":[45],"knock":[47],"down":[48],"expression":[50,70,91],"any":[52],"target":[53],"protein":[54],"introducing":[56],"a":[57,61,83,117],"specific":[58],"siRNA":[59,73],"into":[60,116],"cell.":[62],"Stable":[63],"knockdown":[64,100],"obtained":[67],"constitutive":[69],"from":[74],"host":[76],"chromosome.":[77],"Retroviruses,":[78],"such":[79,103],"as":[80,104,129,131],"lentivirus,":[81,128],"provide":[82],"convenient":[84],"vector":[85],"integrate":[89],"RNAi":[90,147],"constructs.":[92],"Lentiviruses":[93],"infect":[95],"nondividing":[96],"cells,":[97],"thereby":[98],"allowing":[99],"in":[101,141,144],"cells":[102],"mature":[105],"neurons.":[106],"unit":[108],"provides":[109],"methods":[110,135],"design":[112],"clone":[114],"siRNAs":[115],"lentiviral":[118],"vector.":[119],"Additional":[120],"protocols":[121],"describe":[122],"production":[123],"titering":[125],"well":[130],"safety":[132],"testing.":[133],"Finally,":[134],"are":[136],"provided":[137],"for":[138],"infecting":[139],"neurons":[140],"culture":[142],"vivo":[145],"with":[146],"lentivirus.":[148]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}