{"id":"https://openalex.org/W2164028393","doi":null,"title":"Expression and function of heat shock factors in zebrafish (Danio rerio)","display_name":"Expression and function of heat shock factors in zebrafish (Danio rerio)","publication_year":2014,"publication_date":"2014-04-17","ids":{"openalex":"https://openalex.org/W2164028393","mag":"2164028393"},"language":"en","primary_location":{"id":"pmh:oai:ecommons.usask.ca:10388/ETD-2014-04-1492","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/10388/ETD-2014-04-1492","pdf_url":null,"source":{"id":"https://openalex.org/S4377196608","display_name":"University Library - University of Saskatchewan (University of Saskatchewan)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I32625721","host_organization_name":"University of Saskatchewan","host_organization_lineage":["https://openalex.org/I32625721"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5013529236","display_name":"Cynthia L. Swan","orcid":"https://orcid.org/0000-0001-9766-1160","observed_orcids":["https://orcid.org/0000-0001-9766-1160"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Cynthia L. Swan","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0001-9766-1160","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5013529236"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10934","display_name":"Heat shock proteins research","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10934","display_name":"Heat shock proteins research","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10268","display_name":"Developmental Biology and Gene Regulation","score":0.0010000000474974513,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11685","display_name":"Zebrafish Biomedical Research Applications","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/hsf4","display_name":"HSF4","score":0.8550000190734863},{"id":"https://openalex.org/keywords/hsf1","display_name":"HSF1","score":0.9430000185966492},{"id":"https://openalex.org/keywords/hsp70","display_name":"HSP70","score":0.8180000185966492},{"id":"https://openalex.org/keywords/lens-development","display_name":"lens development","score":0.7889999747276306},{"id":"https://openalex.org/keywords/crystalline-lens","display_name":"crystalline lens","score":0.609000027179718},{"id":"https://openalex.org/keywords/zebrafish","display_name":"zebrafish","score":0.8209999799728394},{"id":"https://openalex.org/keywords/morpholino-antisense","display_name":"morpholino antisense","score":0.7369999885559082},{"id":"https://openalex.org/keywords/gene-expression","display_name":"gene expression","score":0.44200000166893005},{"id":"https://openalex.org/keywords/embryonic-development","display_name":"embryonic development","score":0.5529999732971191},{"id":"https://openalex.org/keywords/tissue-specific-expression","display_name":"tissue-specific gene expression","score":0.49399998784065247}],"concepts":[{"id":"https://openalex.org/C2780384893","wikidata":"https://www.wikidata.org/wiki/Q901804","display_name":"Danio","level":4,"score":0.92835533618927},{"id":"https://openalex.org/C2776878037","wikidata":"https://www.wikidata.org/wiki/Q169444","display_name":"Zebrafish","level":3,"score":0.851324200630188},{"id":"https://openalex.org/C14036430","wikidata":"https://www.wikidata.org/wiki/Q3736076","display_name":"Function (biology)","level":2,"score":0.5437965393066406},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.5389012098312378},{"id":"https://openalex.org/C90559484","wikidata":"https://www.wikidata.org/wiki/Q778379","display_name":"Expression (computer science)","level":2,"score":0.485171377658844},{"id":"https://openalex.org/C205260736","wikidata":"https://www.wikidata.org/wiki/Q418939","display_name":"Heat shock protein","level":3,"score":0.44150510430336},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.42393237352371216},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.20035940408706665},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.15816226601600647},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.11667013168334961},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0}],"mesh":[],"locations_count":3,"locations":[{"id":"pmh:oai:ecommons.usask.ca:10388/ETD-2014-04-1492","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/10388/ETD-2014-04-1492","pdf_url":null,"source":{"id":"https://openalex.org/S4377196608","display_name":"University Library - University of Saskatchewan (University of Saskatchewan)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I32625721","host_organization_name":"University of Saskatchewan","host_organization_lineage":["https://openalex.org/I32625721"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"thesis"},{"id":"pmh:oai:harvest.usask.ca:10388/ETD-2014-04-1492","doi":null,"is_oa":false,"landing_page_url":"https://hdl.handle.net/10388/ETD-2014-04-1492","pdf_url":null,"source":{"id":"https://openalex.org/S7407063267","display_name":"HARVEST (University of Saskatchewan)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Thesis"},{"id":"mag:2164028393","doi":null,"is_oa":false,"landing_page_url":"https://harvest.usask.ca/bitstream/10388/ETD-2014-04-1492/3/SWAN-DISSERTATION.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":232,"referenced_works":["https://openalex.org/W42073363","https://openalex.org/W92379874","https://openalex.org/W191130402","https://openalex.org/W192455570","https://openalex.org/W234950505","https://openalex.org/W1208971950","https://openalex.org/W1272416839","https://openalex.org/W1501901620","https://openalex.org/W1529357822","https://openalex.org/W1544206819","https://openalex.org/W1554180547","https://openalex.org/W1555441628","https://openalex.org/W1556198965","https://openalex.org/W1572733245","https://openalex.org/W1577172278","https://openalex.org/W1606142379","https://openalex.org/W1760101923","https://openalex.org/W1836699491","https://openalex.org/W1912190980","https://openalex.org/W1933920814","https://openalex.org/W1950962942","https://openalex.org/W1953084952","https://openalex.org/W1954249603","https://openalex.org/W1959913852","https://openalex.org/W1961074127","https://openalex.org/W1963796435","https://openalex.org/W1966849007","https://openalex.org/W1966958452","https://openalex.org/W1967138582","https://openalex.org/W1967971670","https://openalex.org/W1968780495","https://openalex.org/W1969459260","https://openalex.org/W1972791069","https://openalex.org/W1972896709","https://openalex.org/W1973510010","https://openalex.org/W1973648832","https://openalex.org/W1975195662","https://openalex.org/W1976727208","https://openalex.org/W1977114591","https://openalex.org/W1977442761","https://openalex.org/W1977910682","https://openalex.org/W1978137955","https://openalex.org/W1978565009","https://openalex.org/W1979076435","https://openalex.org/W1979862433","https://openalex.org/W1980291812","https://openalex.org/W1980402014","https://openalex.org/W1980431235","https://openalex.org/W1981048926","https://openalex.org/W1981239255","https://openalex.org/W1981276526","https://openalex.org/W1981501274","https://openalex.org/W1981566059","https://openalex.org/W1982555088","https://openalex.org/W1983832084","https://openalex.org/W1984021854","https://openalex.org/W1984136237","https://openalex.org/W1985549353","https://openalex.org/W1986866379","https://openalex.org/W1987220770","https://openalex.org/W1987296896","https://openalex.org/W1988011051","https://openalex.org/W1989996595","https://openalex.org/W1990040633","https://openalex.org/W1992624136","https://openalex.org/W1992909271","https://openalex.org/W1996781482","https://openalex.org/W1998756807","https://openalex.org/W1999068651","https://openalex.org/W2000286622","https://openalex.org/W2000459271","https://openalex.org/W2001793386","https://openalex.org/W2004798786","https://openalex.org/W2004868450","https://openalex.org/W2005199468","https://openalex.org/W2007515123","https://openalex.org/W2007951643","https://openalex.org/W2008536823","https://openalex.org/W2013803304","https://openalex.org/W2015077514","https://openalex.org/W2015640955","https://openalex.org/W2017606348","https://openalex.org/W2018704517","https://openalex.org/W2019510987","https://openalex.org/W2020517140","https://openalex.org/W2022166667","https://openalex.org/W2024252231","https://openalex.org/W2024457805","https://openalex.org/W2024645073","https://openalex.org/W2024646025","https://openalex.org/W2025871346","https://openalex.org/W2027327809","https://openalex.org/W2027619822","https://openalex.org/W2027688801","https://openalex.org/W2028630156","https://openalex.org/W2029108380","https://openalex.org/W2031793125","https://openalex.org/W2032840272","https://openalex.org/W2035333716","https://openalex.org/W2035470335","https://openalex.org/W2037430182","https://openalex.org/W2037611748","https://openalex.org/W2040235353","https://openalex.org/W2040359453","https://openalex.org/W2040481343","https://openalex.org/W2041979259","https://openalex.org/W2042368077","https://openalex.org/W2042420011","https://openalex.org/W2042953159","https://openalex.org/W2043474702","https://openalex.org/W2044105851","https://openalex.org/W2044652733","https://openalex.org/W2049904722","https://openalex.org/W2052237198","https://openalex.org/W2052374677","https://openalex.org/W2053188090","https://openalex.org/W2053426527","https://openalex.org/W2055007788","https://openalex.org/W2055043387","https://openalex.org/W2057056702","https://openalex.org/W2057079101","https://openalex.org/W2060956856","https://openalex.org/W2061335989","https://openalex.org/W2063117081","https://openalex.org/W2065251407","https://openalex.org/W2066562421","https://openalex.org/W2068011303","https://openalex.org/W2069057607","https://openalex.org/W2070300493","https://openalex.org/W2070501544","https://openalex.org/W2070641117","https://openalex.org/W2071591640","https://openalex.org/W2074573026","https://openalex.org/W2076295042","https://openalex.org/W2078075583","https://openalex.org/W2080082509","https://openalex.org/W2082157468","https://openalex.org/W2084699385","https://openalex.org/W2085331328","https://openalex.org/W2086410486","https://openalex.org/W2089176336","https://openalex.org/W2090174984","https://openalex.org/W2091477850","https://openalex.org/W2091744502","https://openalex.org/W2093304538","https://openalex.org/W2094237591","https://openalex.org/W2097609966","https://openalex.org/W2097685453","https://openalex.org/W2098088378","https://openalex.org/W2099249565","https://openalex.org/W2099952448","https://openalex.org/W2100837269","https://openalex.org/W2101108802","https://openalex.org/W2101911420","https://openalex.org/W2102234720","https://openalex.org/W2102570611","https://openalex.org/W2102653482","https://openalex.org/W2104455852","https://openalex.org/W2104693303","https://openalex.org/W2105254227","https://openalex.org/W2107277218","https://openalex.org/W2107399252","https://openalex.org/W2108222684","https://openalex.org/W2108398067","https://openalex.org/W2108781667","https://openalex.org/W2113117576","https://openalex.org/W2113714901","https://openalex.org/W2117310188","https://openalex.org/W2118292263","https://openalex.org/W2118650779","https://openalex.org/W2119345865","https://openalex.org/W2119624960","https://openalex.org/W2119864607","https://openalex.org/W2120253152","https://openalex.org/W2121699992","https://openalex.org/W2121908416","https://openalex.org/W2122375775","https://openalex.org/W2122457343","https://openalex.org/W2124623372","https://openalex.org/W2124948403","https://openalex.org/W2125013026","https://openalex.org/W2125349610","https://openalex.org/W2125408806","https://openalex.org/W2125828440","https://openalex.org/W2127192432","https://openalex.org/W2128378864","https://openalex.org/W2131356098","https://openalex.org/W2133045719","https://openalex.org/W2135566391","https://openalex.org/W2137168087","https://openalex.org/W2137399444","https://openalex.org/W2137417707","https://openalex.org/W2138876328","https://openalex.org/W2139298045","https://openalex.org/W2139393817","https://openalex.org/W2140733984","https://openalex.org/W2140750082","https://openalex.org/W2143146123","https://openalex.org/W2144889710","https://openalex.org/W2147187641","https://openalex.org/W2147285856","https://openalex.org/W2148970133","https://openalex.org/W2149783560","https://openalex.org/W2150269711","https://openalex.org/W2150510467","https://openalex.org/W2150990088","https://openalex.org/W2153040065","https://openalex.org/W2154209000","https://openalex.org/W2155172429","https://openalex.org/W2155501916","https://openalex.org/W2155623537","https://openalex.org/W2157360354","https://openalex.org/W2159271990","https://openalex.org/W2160803130","https://openalex.org/W2165113979","https://openalex.org/W2165178917","https://openalex.org/W2167479065","https://openalex.org/W2168420558","https://openalex.org/W2168601768","https://openalex.org/W2168861309","https://openalex.org/W2170034660","https://openalex.org/W2171776201","https://openalex.org/W2177598516","https://openalex.org/W2342316354","https://openalex.org/W2344207802","https://openalex.org/W2345994598","https://openalex.org/W2398880538","https://openalex.org/W2408933165","https://openalex.org/W2413335556","https://openalex.org/W2430028249","https://openalex.org/W4298104955","https://openalex.org/W7098362075"],"related_works":["https://openalex.org/W1968523919","https://openalex.org/W1981566059","https://openalex.org/W2177598516","https://openalex.org/W1687718403","https://openalex.org/W1963796435","https://openalex.org/W2008536823","https://openalex.org/W1996781482","https://openalex.org/W2017606348","https://openalex.org/W1968780495","https://openalex.org/W2071591640"],"abstract_inverted_index":{"Heat":[0],"shock":[1,6],"proteins":[2,270,418],"(hsp)":[3],"and":[4,69,93,117,283,293,310,377,385,427],"heat":[5],"transcription":[7,317],"factors":[8,58],"(HSF)":[9],"have":[10,424],"important":[11],"roles":[12],"in":[13,36,41,77,102,130,142,307,311,331,337,369,382,386,399,405],"the":[14,17,103,114,160,180,187,191,200,204,222,301,332,351,432],"development":[15,390],"of":[16,26,54,108,134,151,162,193,203,225,245,268,281,286,300,324,353,360,372,389],"eye":[18,333],"lens.":[19],"Our":[20],"lab":[21],"previously":[22],"demonstrated":[23,220],"that":[24,59,221,275,284,363,409],"knockdown":[25,53,359],"hsp70":[27,61],"gene":[28],"expression":[29,120,296,379,428],"using":[30,140],"morpholino":[31],"antisense":[32],"technology":[33],"(MO)":[34],"resulted":[35],"a":[37,75,94,172,265,367],"small":[38],"lens":[39,65,79,131,373],"phenotype":[40,49,356],"zebrafish":[42,86,115,135,176,226,246,292,303,400,411],"(Danio":[43],"rerio)":[44],"embryos.":[45],"A":[46],"less":[47],"severe":[48],"was":[50,138,251,277,305,328],"seen":[51],"with":[52,156,190,264,350,416],"hsf1,":[55],"suggesting":[56],"other":[57,122,238,258,420],"regulate":[60],"are":[62,71],"involved":[63],"during":[64],"formation.":[66],"Both":[67],"HSF1":[68,235,256,276],"HSF4":[70,116,136,158,227,239,247,259,282,364],"known":[72],"to":[73,112,121,171,179,186,228,234,237,255,431],"play":[74],"role":[76,129,368],"mammalian":[78,157,181,212],"development.":[80,132,374],"An":[81],"expressed":[82],"sequence":[83,137,150,189,244],"tag":[84],"encoding":[85],"HSF4,":[87],"named":[88],"hsf4a,":[89],"has":[90,99,366],"been":[91,100],"identified":[92],"second":[95],"splice":[96],"variant,":[97],"hsf4b,":[98],"predicted":[101],"Ensembl":[104],"database.":[105],"The":[106,146,241,295,402],"objectives":[107],"this":[109,287,406],"thesis":[110,407],"were":[111,380],"characterize":[113],"compare":[118],"its":[119,128],"HSFs":[123,304,412],"as":[124,126,391,393,396],"well":[125,392],"investigate":[127],"Analysis":[133],"performed":[139],"standard":[141],"silico":[143],"analytical":[144],"software.":[145],"deduced":[147],"amino":[148,197,242],"acid":[149,243],"HSF4a":[152,177,188],"shares":[153],"structural":[154,425],"similarities":[155],"including":[159],"lack":[161,352],"an":[163,194,208,278,354],"HR-C":[164,209],"domain.":[165],"This":[166],"domain":[167,250],"is":[168,184,231],"absent":[169],"due":[170],"C-terminal":[173],"truncation":[174],"within":[175],"relative":[178],"protein.":[182],"HSF4b":[183],"identical":[185],"exception":[192],"additional":[195],"155":[196],"acids":[198],"at":[199,341],"carboxyl":[201],"end":[202],"protein":[205,288],"which":[206],"contains":[207],"domain,":[210],"unlike":[211],"HSF4.":[213],"Surprisingly,":[214],"electrophoretic":[215],"mobility":[216],"shift":[217],"assays":[218],"(EMSA)":[219],"binding":[223,249],"affinity":[224],"discontinuous":[229],"HSEs":[230],"more":[232,253],"similar":[233,254],"than":[236,257],"proteins.":[240,260],"DNA":[248],"also":[252,423],"These":[261],"results,":[262],"along":[263],"phylogenetic":[266],"analysis":[267],"HSF":[269,417],"from":[271,419],"eleven":[272],"species,":[273,421],"suggest":[274],"evolutionary":[279],"precursor":[280],"functions":[285],"may":[289],"differ":[290],"between":[291],"mammals.":[294],"level":[297],"for":[298],"each":[299],"three":[302],"determined":[306],"adult":[308],"tissues":[309,384],"developing":[312,338],"embryos":[313],"by":[314],"quantitative":[315],"reverse":[316],"polymerase":[318],"chain":[319],"reaction":[320],"(qPCR)":[321],"analysis.":[322],"Expression":[323],"both":[325],"hsf4":[326],"transcripts":[327,398],"observed":[329,336],"predominantly":[330],"but":[334],"only":[335],"embryonic":[339],"tissue":[340],"60":[342],"hours":[343],"post":[344],"fertilization":[345],"or":[346],"later.":[347],"This,":[348],"together":[349],"observable":[355],"following":[357],"MO":[358],"hsf4,":[361],"suggests":[362],"likely":[365],"later":[370],"stages":[371,388],"Additionally,":[375],"hsf1":[376],"hsf2":[378],"detected":[381],"all":[383,387],"being":[394],"present":[395],"maternal":[397],"eggs.":[401],"results":[403],"presented":[404],"demonstrate":[408],"while":[410],"share":[413],"some":[414],"similarity":[415],"they":[422],"characteristics":[426],"patterns":[429],"unique":[430],"zebrafish.":[433]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}