{"id":"https://openalex.org/W2163887638","doi":null,"title":"Induced secretion of a Mr 46,000 protein by cultured human hepatoma cells treated with tumor promoters.","display_name":"Induced secretion of a Mr 46,000 protein by cultured human hepatoma cells treated with tumor promoters.","publication_year":1985,"publication_date":"1985-05-01","ids":{"openalex":"https://openalex.org/W2163887638","mag":"2163887638","pmid":"https://pubmed.ncbi.nlm.nih.gov/2985250"},"language":"en","primary_location":{"id":"pmid:2985250","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2985250","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer research","raw_type":"Journal Article"},"volume":null,"type":"article","indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5112152638","display_name":"Yoshihiro Yoneda","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Y Yoneda","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5028634203","display_name":"Hirota Fujiki","orcid":"https://orcid.org/0000-0002-1512-3551","observed_orcids":["https://orcid.org/0000-0002-1512-3551"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"H Fujiki","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5107595054","display_name":"Richard E. Moore","orcid":"https://orcid.org/0000-0002-5328-9018","observed_orcids":["https://orcid.org/0000-0002-5328-9018"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"R E Moore","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5025873590","display_name":"Tsuyoshi Uchida","orcid":"https://orcid.org/0000-0002-5462-6565","observed_orcids":["https://orcid.org/0000-0002-5462-6565"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"T Uchida","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.4404,"has_fulltext":false,"cited_by_count":3,"citation_normalized_percentile":{"value":0.68256938,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"45","issue":"5","first_page":"2863","last_page":"8"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10073","display_name":"Hepatocellular Carcinoma Treatment and Prognosis","score":0.30979999899864197,"subfield":{"id":"https://openalex.org/subfields/2721","display_name":"Hepatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10073","display_name":"Hepatocellular Carcinoma Treatment and Prognosis","score":0.30979999899864197,"subfield":{"id":"https://openalex.org/subfields/2721","display_name":"Hepatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10966","display_name":"Liver physiology and pathology","score":0.1111999973654747,"subfield":{"id":"https://openalex.org/subfields/2721","display_name":"Hepatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.06449999660253525,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/hepatoma-cells","display_name":"hepatoma cells","score":0.5910000205039978},{"id":"https://openalex.org/keywords/tumor-promoters","display_name":"tumor promoters","score":0.7549999952316284},{"id":"https://openalex.org/keywords/phorbol-myristate-acetate","display_name":"phorbol myristate acetate","score":0.9750000238418579},{"id":"https://openalex.org/keywords/teleocidin","display_name":"teleocidin","score":0.9729999899864197},{"id":"https://openalex.org/keywords/aplysiatoxin","display_name":"aplysiatoxin","score":0.9919999837875366},{"id":"https://openalex.org/keywords/debromoaplysiatoxin","display_name":"debromoaplysiatoxin","score":0.9980000257492065},{"id":"https://openalex.org/keywords/phorbol-ester","display_name":"phorbol ester","score":0.9380000233650208},{"id":"https://openalex.org/keywords/protein-secretion","display_name":"protein secretion","score":0.8479999899864197},{"id":"https://openalex.org/keywords/protein-isoforms","display_name":"protein isoforms","score":0.8199999928474426},{"id":"https://openalex.org/keywords/de-novo-rna-synthesis","display_name":"de novo RNA synthesis","score":0.7940000295639038},{"id":"https://openalex.org/keywords/dactinomycin","display_name":"actinomycin D","score":0.9549999833106995}],"concepts":[{"id":"https://openalex.org/C49039625","wikidata":"https://www.wikidata.org/wiki/Q84230","display_name":"Secretion","level":2,"score":0.6365424394607544},{"id":"https://openalex.org/C50476208","wikidata":"https://www.wikidata.org/wiki/Q898861","display_name":"Isoelectric focusing","level":3,"score":0.6243507862091064},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.6097043752670288},{"id":"https://openalex.org/C116084860","wikidata":"https://www.wikidata.org/wiki/Q48255","display_name":"Gel electrophoresis","level":2,"score":0.5959911942481995},{"id":"https://openalex.org/C2780912031","wikidata":"https://www.wikidata.org/wiki/Q180341","display_name":"Methionine","level":3,"score":0.5877233743667603},{"id":"https://openalex.org/C101660505","wikidata":"https://www.wikidata.org/wiki/Q898301","display_name":"Polyacrylamide gel electrophoresis","level":3,"score":0.539036750793457},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5195755958557129},{"id":"https://openalex.org/C2778913249","wikidata":"https://www.wikidata.org/wiki/Q422241","display_name":"Sodium dodecyl sulfate","level":2,"score":0.5107409954071045},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.48080363869667053},{"id":"https://openalex.org/C91523082","wikidata":"https://www.wikidata.org/wiki/Q420496","display_name":"Antiserum","level":3,"score":0.4807150661945343},{"id":"https://openalex.org/C96294017","wikidata":"https://www.wikidata.org/wiki/Q308708","display_name":"Isoelectric point","level":3,"score":0.4803232252597809},{"id":"https://openalex.org/C2780043322","wikidata":"https://www.wikidata.org/wiki/Q2705359","display_name":"Phorbol","level":4,"score":0.47221237421035767},{"id":"https://openalex.org/C156399914","wikidata":"https://www.wikidata.org/wiki/Q7444454","display_name":"Secretory protein","level":3,"score":0.41803646087646484},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.14423641562461853},{"id":"https://openalex.org/C195794163","wikidata":"https://www.wikidata.org/wiki/Q420877","display_name":"Protein kinase C","level":3,"score":0.13358399271965027},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.09439468383789062},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.09122619032859802},{"id":"https://openalex.org/C11960822","wikidata":"https://www.wikidata.org/wiki/Q242736","display_name":"Phosphorylation","level":2,"score":0.09056895971298218},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.05506026744842529}],"mesh":[{"descriptor_ui":"D002273","descriptor_name":"Carcinogens","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003609","descriptor_name":"Dactinomycin","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D006528","descriptor_name":"Carcinoma, Hepatocellular","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008113","descriptor_name":"Liver Neoplasms","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D008970","descriptor_name":"Molecular Weight","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009363","descriptor_name":"Neoplasm Proteins","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D009363","descriptor_name":"Neoplasm Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D013755","descriptor_name":"Tetradecanoylphorbol Acetate","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014158","descriptor_name":"Transcription, Genetic","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false}],"locations_count":3,"locations":[{"id":"pmid:2985250","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2985250","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer research","raw_type":"Journal Article"},{"id":"mag:2163887638","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2985250","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null},{"id":"mag:2296610685","doi":null,"is_oa":false,"landing_page_url":"http://ci.nii.ac.jp/naid/500000013520","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.8560000061988831}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":38,"referenced_works":["https://openalex.org/W256887666","https://openalex.org/W1516259788","https://openalex.org/W1524297452","https://openalex.org/W1594758412","https://openalex.org/W1606423922","https://openalex.org/W1612024825","https://openalex.org/W1969699638","https://openalex.org/W1974096267","https://openalex.org/W1976750101","https://openalex.org/W1977382255","https://openalex.org/W1977521635","https://openalex.org/W1981018920","https://openalex.org/W1983399566","https://openalex.org/W1994233203","https://openalex.org/W1999689481","https://openalex.org/W2001692359","https://openalex.org/W2012866181","https://openalex.org/W2013028390","https://openalex.org/W2015544430","https://openalex.org/W2034277598","https://openalex.org/W2034752256","https://openalex.org/W2038306032","https://openalex.org/W2043037423","https://openalex.org/W2047747599","https://openalex.org/W2058901530","https://openalex.org/W2063183684","https://openalex.org/W2063475148","https://openalex.org/W2064762154","https://openalex.org/W2093978003","https://openalex.org/W2095119698","https://openalex.org/W2099825211","https://openalex.org/W2100837269","https://openalex.org/W2116952818","https://openalex.org/W2122601779","https://openalex.org/W2168138638","https://openalex.org/W2383007117","https://openalex.org/W2417135617","https://openalex.org/W2424195085"],"related_works":["https://openalex.org/W303358997","https://openalex.org/W2116952818","https://openalex.org/W2168138638","https://openalex.org/W3105372281","https://openalex.org/W2086736998","https://openalex.org/W2089196790","https://openalex.org/W2394714062","https://openalex.org/W2399528350","https://openalex.org/W1587318143","https://openalex.org/W2043483755"],"abstract_inverted_index":{"Human":[0],"hepatoma":[1],"cells,":[2],"HuH-6":[3],"Cl-5,":[4],"were":[5,62,189,204],"treated":[6,190],"with":[7,50,191],"12-O-tetradecanoylphorbol-13-acetate":[8],"at":[9],"concentrations":[10],"of":[11,23,32,47,54,72,84,93,99,118,139,158,194],"1":[12],"ng/ml":[13],"to":[14,182],"10":[15],"micrograms/ml":[16],"for":[17],"6":[18],"h":[19],"in":[20],"the":[21,33,38,59,97,156,161,185],"presence":[22],"[35S]methionine.":[24],"Analysis":[25],"by":[26,64,102,109,174],"sodium":[27],"dodecyl":[28],"sulfate-polyacrylamide":[29],"gel":[30,67],"electrophoresis":[31],"labeled":[34,60],"proteins":[35,61,187,203],"secreted":[36],"into":[37,160],"medium":[39],"showed":[40],"that":[41,107,166],"this":[42,94],"treatment":[43],"induced":[44,91,101,108,173],"marked":[45],"secretion":[46,92,141],"a":[48,51,121,170],"polypeptide":[49],"molecular":[52],"weight":[53],"about":[55],"46,000":[56],"(p46).":[57],"When":[58],"analyzed":[63],"two-dimensional":[65],"polyacrylamide":[66],"electrophoresis,":[68],"p46":[69,100,140,167,206],"was":[70,104,168,207],"composed":[71],"three":[73],"isoproteins":[74],"which":[75],"had":[76],"different":[77],"isoelectric":[78],"points.":[79],"The":[80,123,137],"two":[81,192],"new":[82],"classes":[83],"tumor":[85,177],"promoters,":[86],"teleocidin":[87],"and":[88,112,131,153],"aplysiatoxin,":[89,113],"also":[90],"protein,":[95],"although":[96,201],"amount":[98],"debromoaplysiatoxin":[103],"less":[105],"than":[106],"12-O-tetradecanoylphorbol-13-acetate,":[110],"teleocidin,":[111],"judging":[114],"from":[115],"densitometric":[116],"scanning":[117],"bands":[119],"on":[120],"fluorogram.":[122],"nonpromoting":[124],"phorbol":[125],"esters,":[126],"such":[127],"as":[128],"4":[129],"beta-phorbol":[130],"phorbol-13-monoacetate,":[132],"did":[133],"not":[134,169],"induce":[135],"p46.":[136],"induction":[138],"involved":[142],"de":[143],"novo":[144],"RNA":[145],"synthesis,":[146],"since":[147],"actinomycin":[148],"D":[149],"(1":[150],"microgram/ml)":[151],"completely":[152],"selectively":[154],"blocked":[155],"incorporation":[157],"[35S]-methionine":[159],"protein.":[162],"\"Pulse-chase\"":[163],"experiments":[164],"indicated":[165],"degradation":[171],"product":[172],"these":[175],"potent":[176],"promoters.":[178],"In":[179],"an":[180],"attempt":[181],"identify":[183],"p46,":[184],"total":[186],"released":[188],"kinds":[193],"rabbit":[195],"anti-human":[196],"whole":[197],"serum":[198],"antisera,":[199],"but":[200],"some":[202],"precipitated,":[205],"not.":[208]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}