{"id":"https://openalex.org/W2163737726","doi":"https://doi.org/10.1128/cvi.05715-11","title":"Impact of Human Granulocyte and Monocyte Isolation Procedures on Functional Studies","display_name":"Impact of Human Granulocyte and Monocyte Isolation Procedures on Functional Studies","publication_year":2012,"publication_date":"2012-05-02","ids":{"openalex":"https://openalex.org/W2163737726","doi":"https://doi.org/10.1128/cvi.05715-11","mag":"2163737726","pmid":"https://pubmed.ncbi.nlm.nih.gov/22552601"},"language":"en","primary_location":{"id":"doi:10.1128/cvi.05715-11","doi":"https://doi.org/10.1128/cvi.05715-11","is_oa":true,"landing_page_url":"https://doi.org/10.1128/cvi.05715-11","pdf_url":null,"source":{"id":"https://openalex.org/S114575275","display_name":"Clinical and Vaccine Immunology","issn_l":"1556-679X","issn":["1556-679X","1556-6811"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","norway-1","poland-100"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Clinical and Vaccine Immunology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"https://doi.org/10.1128/cvi.05715-11","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5086104464","display_name":"Lu Zhou","orcid":"https://orcid.org/0000-0002-0424-7348","observed_orcids":["https://orcid.org/0000-0002-0424-7348","https://orcid.org/0000-0002-2688-7135","https://orcid.org/0000-0002-5464-5176"]},"institutions":[{"id":"https://openalex.org/I1334415907","display_name":"University Medical Center Groningen","ror":"https://ror.org/03cv38k47","country_code":"NL","type":"healthcare","lineage":["https://openalex.org/I1334415907"]},{"id":"https://openalex.org/I169381384","display_name":"University of Groningen","ror":"https://ror.org/012p63287","country_code":"NL","type":"education","lineage":["https://openalex.org/I169381384"]},{"id":"https://openalex.org/I2802534033","display_name":"Tianjin Medical University General Hospital","ror":"https://ror.org/003sav965","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I2802534033"]},{"id":"https://openalex.org/I5740404","display_name":"Tianjin Medical University","ror":"https://ror.org/02mh8wx89","country_code":"CN","type":"education","lineage":["https://openalex.org/I5740404"]}],"countries":["CN","NL"],"is_corresponding":false,"raw_author_name":"Lu Zhou","raw_affiliation_strings":["Department of Gastroenterology and Hepatology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands","Department of Gastroenterology and Hepatology, Tianjin Medical University General Hospital, Tianjin Medical University, Tianjin, People's Republic of China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Gastroenterology and Hepatology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands","institution_ids":["https://openalex.org/I1334415907","https://openalex.org/I169381384"]},{"raw_affiliation_string":"Department of Gastroenterology and Hepatology, Tianjin Medical University General Hospital, Tianjin Medical University, Tianjin, People's Republic of China","institution_ids":["https://openalex.org/I2802534033","https://openalex.org/I5740404"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5066870276","display_name":"Rajesh Somasundaram","orcid":"https://orcid.org/0000-0003-0669-9644","observed_orcids":["https://orcid.org/0000-0003-0669-9644"]},"institutions":[{"id":"https://openalex.org/I2801952686","display_name":"Erasmus MC","ror":"https://ror.org/018906e22","country_code":"NL","type":"healthcare","lineage":["https://openalex.org/I2801952686"]},{"id":"https://openalex.org/I913958620","display_name":"Erasmus University Rotterdam","ror":"https://ror.org/057w15z03","country_code":"NL","type":"education","lineage":["https://openalex.org/I913958620"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"Rajesh Somasundaram","raw_affiliation_strings":["Department of Gastroenterology and Hepatology, Erasmus Medical Center, Erasmus University of Rotterdam, Rotterdam, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Gastroenterology and Hepatology, Erasmus Medical Center, Erasmus University of Rotterdam, Rotterdam, The Netherlands","institution_ids":["https://openalex.org/I2801952686","https://openalex.org/I913958620"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5045363176","display_name":"Rosa F. Nederhof","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1334415907","display_name":"University Medical Center Groningen","ror":"https://ror.org/03cv38k47","country_code":"NL","type":"healthcare","lineage":["https://openalex.org/I1334415907"]},{"id":"https://openalex.org/I169381384","display_name":"University of Groningen","ror":"https://ror.org/012p63287","country_code":"NL","type":"education","lineage":["https://openalex.org/I169381384"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"Rosa F. Nederhof","raw_affiliation_strings":["Department of Gastroenterology and Hepatology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Gastroenterology and Hepatology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands","institution_ids":["https://openalex.org/I1334415907","https://openalex.org/I169381384"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5068944242","display_name":"Gerard D. H. Dijkstra","orcid":"https://orcid.org/0000-0003-4563-7462","observed_orcids":["https://orcid.org/0000-0003-4563-7462"]},"institutions":[{"id":"https://openalex.org/I1334415907","display_name":"University Medical Center Groningen","ror":"https://ror.org/03cv38k47","country_code":"NL","type":"healthcare","lineage":["https://openalex.org/I1334415907"]},{"id":"https://openalex.org/I169381384","display_name":"University of Groningen","ror":"https://ror.org/012p63287","country_code":"NL","type":"education","lineage":["https://openalex.org/I169381384"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"Gerard Dijkstra","raw_affiliation_strings":["Department of Gastroenterology and Hepatology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Gastroenterology and Hepatology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands","institution_ids":["https://openalex.org/I1334415907","https://openalex.org/I169381384"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5001756705","display_name":"Klaas Nico Faber","orcid":"https://orcid.org/0000-0001-8893-3312","observed_orcids":["https://orcid.org/0000-0001-8893-3312"]},"institutions":[{"id":"https://openalex.org/I1334415907","display_name":"University Medical Center Groningen","ror":"https://ror.org/03cv38k47","country_code":"NL","type":"healthcare","lineage":["https://openalex.org/I1334415907"]},{"id":"https://openalex.org/I169381384","display_name":"University of Groningen","ror":"https://ror.org/012p63287","country_code":"NL","type":"education","lineage":["https://openalex.org/I169381384"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"Klaas Nico Faber","raw_affiliation_strings":["Department of Gastroenterology and Hepatology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Gastroenterology and Hepatology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands","institution_ids":["https://openalex.org/I1334415907","https://openalex.org/I169381384"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5069908102","display_name":"Maikel Petrus Peppelenbosch","orcid":"https://orcid.org/0000-0001-9112-6028","observed_orcids":["https://orcid.org/0000-0001-9112-6028"]},"institutions":[{"id":"https://openalex.org/I2801952686","display_name":"Erasmus MC","ror":"https://ror.org/018906e22","country_code":"NL","type":"healthcare","lineage":["https://openalex.org/I2801952686"]},{"id":"https://openalex.org/I913958620","display_name":"Erasmus University Rotterdam","ror":"https://ror.org/057w15z03","country_code":"NL","type":"education","lineage":["https://openalex.org/I913958620"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"Maikel P. Peppelenbosch","raw_affiliation_strings":["Department of Gastroenterology and Hepatology, Erasmus Medical Center, Erasmus University of Rotterdam, Rotterdam, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Gastroenterology and Hepatology, Erasmus Medical Center, Erasmus University of Rotterdam, Rotterdam, The Netherlands","institution_ids":["https://openalex.org/I2801952686","https://openalex.org/I913958620"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5058386574","display_name":"Gwenny Manel Fuhler","orcid":"https://orcid.org/0000-0001-9221-4855","observed_orcids":["https://orcid.org/0000-0001-9221-4855"]},"institutions":[{"id":"https://openalex.org/I2801952686","display_name":"Erasmus MC","ror":"https://ror.org/018906e22","country_code":"NL","type":"healthcare","lineage":["https://openalex.org/I2801952686"]},{"id":"https://openalex.org/I913958620","display_name":"Erasmus University Rotterdam","ror":"https://ror.org/057w15z03","country_code":"NL","type":"education","lineage":["https://openalex.org/I913958620"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"Gwenny M. Fuhler","raw_affiliation_strings":["Department of Gastroenterology and Hepatology, Erasmus Medical Center, Erasmus University of Rotterdam, Rotterdam, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Gastroenterology and Hepatology, Erasmus Medical Center, Erasmus University of Rotterdam, Rotterdam, The Netherlands","institution_ids":["https://openalex.org/I2801952686","https://openalex.org/I913958620"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":6,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":3.0791,"has_fulltext":false,"cited_by_count":108,"citation_normalized_percentile":{"value":0.91866792,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":99},"biblio":{"volume":"19","issue":"7","first_page":"1065","last_page":"1074"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11597","display_name":"Neutrophil, Myeloperoxidase and Oxidative Mechanisms","score":0.6973000168800354,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11597","display_name":"Neutrophil, Myeloperoxidase and Oxidative Mechanisms","score":0.6973000168800354,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10371","display_name":"Immune Response and Inflammation","score":0.15549999475479126,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10031","display_name":"T-cell and B-cell Immunology","score":0.02019999921321869,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/monocyte-isolation","display_name":"monocyte isolation","score":0.9950000047683716},{"id":"https://openalex.org/keywords/positive-selection","display_name":"positive selection","score":0.7149999737739563},{"id":"https://openalex.org/keywords/magnetic-microbeads","display_name":"magnetic microbeads","score":0.7300000190734863},{"id":"https://openalex.org/keywords/ficoll-density-gradient","display_name":"Ficoll density gradient","score":0.8109999895095825},{"id":"https://openalex.org/keywords/anti-cd14-antibody","display_name":"anti-CD14 antibody","score":0.8949999809265137},{"id":"https://openalex.org/keywords/cell-surface-receptor-expression","display_name":"cell surface receptor expression","score":0.6819999814033508},{"id":"https://openalex.org/keywords/respiratory-burst","display_name":"respiratory burst","score":0.8259999752044678},{"id":"https://openalex.org/keywords/phagocytosis","display_name":"phagocytosis","score":0.9819999933242798},{"id":"https://openalex.org/keywords/innate-immunity","display_name":"innate immunity","score":0.8840000033378601},{"id":"https://openalex.org/keywords/autoimmune-disease","display_name":"autoimmune disease","score":0.7670000195503235},{"id":"https://openalex.org/keywords/cell-purity","display_name":"cell purity","score":0.4620000123977661}],"concepts":[{"id":"https://openalex.org/C2777983669","wikidata":"https://www.wikidata.org/wiki/Q223143","display_name":"Granulocyte","level":2,"score":0.7670364379882812},{"id":"https://openalex.org/C2781184567","wikidata":"https://www.wikidata.org/wiki/Q107244","display_name":"Monocyte","level":2,"score":0.6457840204238892},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.6216458678245544},{"id":"https://openalex.org/C2778513237","wikidata":"https://www.wikidata.org/wiki/Q419553","display_name":"CD14","level":3,"score":0.5883466601371765},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.5430007576942444},{"id":"https://openalex.org/C136449434","wikidata":"https://www.wikidata.org/wiki/Q428253","display_name":"Innate immune system","level":3,"score":0.525400698184967},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.49956512451171875},{"id":"https://openalex.org/C8891405","wikidata":"https://www.wikidata.org/wiki/Q1059","display_name":"Immune system","level":2,"score":0.4946272075176239},{"id":"https://openalex.org/C5362183","wikidata":"https://www.wikidata.org/wiki/Q410200","display_name":"Ficoll","level":4,"score":0.4314676821231842},{"id":"https://openalex.org/C137061746","wikidata":"https://www.wikidata.org/wiki/Q736033","display_name":"Peripheral blood mononuclear cell","level":3,"score":0.14185813069343567},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.12653490900993347},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.07512548565864563}],"mesh":[{"descriptor_ui":"D002469","descriptor_name":"Cell Separation","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D002469","descriptor_name":"Cell Separation","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D002469","descriptor_name":"Cell Separation","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D006098","descriptor_name":"Granulocytes","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D006098","descriptor_name":"Granulocytes","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D006098","descriptor_name":"Granulocytes","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D006098","descriptor_name":"Granulocytes","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D006098","descriptor_name":"Granulocytes","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D006098","descriptor_name":"Granulocytes","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006805","descriptor_name":"Human Experimentation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006805","descriptor_name":"Human Experimentation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006805","descriptor_name":"Human Experimentation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009000","descriptor_name":"Monocytes","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D009000","descriptor_name":"Monocytes","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D009000","descriptor_name":"Monocytes","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D009000","descriptor_name":"Monocytes","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D009000","descriptor_name":"Monocytes","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D009000","descriptor_name":"Monocytes","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D010587","descriptor_name":"Phagocytosis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010587","descriptor_name":"Phagocytosis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010587","descriptor_name":"Phagocytosis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011971","descriptor_name":"Receptors, Immunologic","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D011971","descriptor_name":"Receptors, Immunologic","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D011971","descriptor_name":"Receptors, Immunologic","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D013048","descriptor_name":"Specimen Handling","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D013048","descriptor_name":"Specimen Handling","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D013048","descriptor_name":"Specimen Handling","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D016897","descriptor_name":"Respiratory Burst","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016897","descriptor_name":"Respiratory Burst","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016897","descriptor_name":"Respiratory Burst","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":10,"locations":[{"id":"doi:10.1128/cvi.05715-11","doi":"https://doi.org/10.1128/cvi.05715-11","is_oa":true,"landing_page_url":"https://doi.org/10.1128/cvi.05715-11","pdf_url":null,"source":{"id":"https://openalex.org/S114575275","display_name":"Clinical and Vaccine Immunology","issn_l":"1556-679X","issn":["1556-679X","1556-6811"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","norway-1","poland-100"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Clinical and Vaccine Immunology","raw_type":"journal-article"},{"id":"pmid:22552601","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/22552601","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Clinical and vaccine immunology : CVI","raw_type":"Journal Article"},{"id":"pmh:oai:pure.eur.nl:openaire_cris_publications/5d09f2ef-bb4f-4100-b7c0-e6ed0bb6c15b","doi":null,"is_oa":false,"landing_page_url":"https://pure.eur.nl/en/publications/5d09f2ef-bb4f-4100-b7c0-e6ed0bb6c15b","pdf_url":null,"source":{"id":"https://openalex.org/S4306401266","display_name":"EUR Research Repository (Erasmus University Rotterdam)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I913958620","host_organization_name":"Erasmus University Rotterdam","host_organization_lineage":["https://openalex.org/I913958620"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Zhou, L, Somasundaram, R, Nederhof, RF, Dijkstra, G, Faber, KN, Peppelenbosch, M & Fuhler, G 2012, 'Impact of Human Granulocyte and Monocyte Isolation Procedures on Functional Studies', Clinical and Vaccine Immunology, vol. 19, no. 7, pp. 1065-1074. https://doi.org/10.1128/CVI.05715-11","raw_type":"info:eu-repo/semantics/article"},{"id":"pmh:oai:europepmc.org:2532613","doi":null,"is_oa":false,"landing_page_url":"http://europepmc.org/articles/PMC3393372","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:3393372","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3393372","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Clin Vaccine Immunol","raw_type":"Text"},{"id":"pmh:eur:oai:pure.eur.nl:publications/5d09f2ef-bb4f-4100-b7c0-e6ed0bb6c15b","doi":null,"is_oa":false,"landing_page_url":"https://pure.eur.nl/en/publications/5d09f2ef-bb4f-4100-b7c0-e6ed0bb6c15b","pdf_url":null,"source":{"id":"https://openalex.org/S4306401266","display_name":"EUR Research Repository (Erasmus University Rotterdam)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I913958620","host_organization_name":"Erasmus University Rotterdam","host_organization_lineage":["https://openalex.org/I913958620"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Clinical and Vaccine Immunology, 19(7), 1065 - 1074. American Society for Microbiology","raw_type":"info:eu-repo/semantics/article"},{"id":"pmh:eur:oai:repub.eur.nl:61351","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/1765/61351","pdf_url":null,"source":{"id":"https://openalex.org/S4377196287","display_name":"RePub (Erasmus University Rotterdam)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I913958620","host_organization_name":"Erasmus University Rotterdam","host_organization_lineage":["https://openalex.org/I913958620"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Clinical and Vaccine Immunology (Print), 19(7), 1065 - 1074","raw_type":"info:eu-repo/semantics/article"},{"id":"pmh:oai:pure.rug.nl:openaire_cris_publications/0a2676bf-830f-497e-a0b9-1c81c4a934ab","doi":null,"is_oa":false,"landing_page_url":"https://hdl.handle.net/11370/0a2676bf-830f-497e-a0b9-1c81c4a934ab","pdf_url":null,"source":{"id":"https://openalex.org/S4306400420","display_name":"University of Groningen research database (University of Groningen / Centre for Information Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I169381384","host_organization_name":"University of Groningen","host_organization_lineage":["https://openalex.org/I169381384"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Zhou, L, Somasundaram, R, Nederhof, R F, Dijkstra, G, Faber, K N, Peppelenbosch, M P & Fuhler, G M 2012, 'Impact of Human Granulocyte and Monocyte Isolation Procedures on Functional Studies', Clinical and vaccine immunology, vol. 19, no. 7, pp. 1065-1074. https://doi.org/10.1128/CVI.05715-11","raw_type":"info:eu-repo/semantics/article"},{"id":"pmh:oai:repub.eur.nl:61351","doi":null,"is_oa":true,"landing_page_url":"http://repub.eur.nl/pub/61351","pdf_url":null,"source":{"id":"https://openalex.org/S4377196287","display_name":"RePub (Erasmus University Rotterdam)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I913958620","host_organization_name":"Erasmus University Rotterdam","host_organization_lineage":["https://openalex.org/I913958620"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Clinical and Vaccine Immunology (Print) vol. 19 no. 7, pp. 1065-1074","raw_type":"info:eu-repo/semantics/article"},{"id":"pmh:rug:oai:pure.rug.nl:publications/0a2676bf-830f-497e-a0b9-1c81c4a934ab","doi":null,"is_oa":false,"landing_page_url":"https://research.rug.nl/en/publications/0a2676bf-830f-497e-a0b9-1c81c4a934ab","pdf_url":null,"source":{"id":"https://openalex.org/S4306400420","display_name":"University of Groningen research database (University of Groningen / Centre for Information Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I169381384","host_organization_name":"University of Groningen","host_organization_lineage":["https://openalex.org/I169381384"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Clinical and vaccine immunology, 19(7), 1065 - 1074","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":{"id":"doi:10.1128/cvi.05715-11","doi":"https://doi.org/10.1128/cvi.05715-11","is_oa":true,"landing_page_url":"https://doi.org/10.1128/cvi.05715-11","pdf_url":null,"source":{"id":"https://openalex.org/S114575275","display_name":"Clinical and Vaccine Immunology","issn_l":"1556-679X","issn":["1556-679X","1556-6811"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","norway-1","poland-100"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Clinical and Vaccine Immunology","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.9763000011444092}],"sustainable_development_goals_aurora":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.7699999809265137}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":30,"referenced_works":["https://openalex.org/W1563659500","https://openalex.org/W1615858282","https://openalex.org/W1638726768","https://openalex.org/W1971046821","https://openalex.org/W1994017097","https://openalex.org/W1998094992","https://openalex.org/W2007493927","https://openalex.org/W2008575683","https://openalex.org/W2012136117","https://openalex.org/W2025990113","https://openalex.org/W2029617490","https://openalex.org/W2038118874","https://openalex.org/W2040992929","https://openalex.org/W2044288837","https://openalex.org/W2048612489","https://openalex.org/W2061512720","https://openalex.org/W2067022007","https://openalex.org/W2084263070","https://openalex.org/W2090936815","https://openalex.org/W2093597913","https://openalex.org/W2099055805","https://openalex.org/W2124823452","https://openalex.org/W2128175802","https://openalex.org/W2129716877","https://openalex.org/W2134422149","https://openalex.org/W2138276685","https://openalex.org/W2142599884","https://openalex.org/W2143918668","https://openalex.org/W2169951875","https://openalex.org/W2199143320"],"related_works":["https://openalex.org/W2368671469","https://openalex.org/W2476067827","https://openalex.org/W2562018129","https://openalex.org/W4378745089","https://openalex.org/W272206334","https://openalex.org/W2420872905","https://openalex.org/W2966345944","https://openalex.org/W3153646899","https://openalex.org/W1584783298","https://openalex.org/W2190014693"],"abstract_inverted_index":{"One":[0],"of":[1,5,12,53,76,84,151,172,214,223,227,239],"the":[2,10,13,45,69,77,82,142,183,221],"first":[3],"lines":[4],"defense":[6],"against":[7],"infection":[8],"is":[9,18,58],"activation":[11],"innate":[14,36],"immune":[15,86],"system.":[16],"It":[17],"becoming":[19],"clear":[20],"that":[21,211],"autoimmune":[22],"diseases,":[23],"such":[24],"as":[25,220],"rheumatoid":[26],"arthritis":[27],"and":[28,38,41,71,113,140,153,168,216,230],"Crohn's":[29],"disease,":[30],"may":[31],"be":[32,218,237],"caused":[33],"by":[34,130,161],"disturbed":[35],"immunity,":[37],"relating":[39],"granulocyte":[40,96,184,196,229],"monocyte":[42,231],"functions":[43,232],"to":[44,60,204,236],"patient":[46],"genotype":[47],"has":[48,88],"become":[49],"an":[50],"important":[51],"part":[52],"contemporary":[54],"research.":[55],"Although":[56],"it":[57],"essential":[59],"move":[61],"this":[62],"field":[63],"forward,":[64],"a":[65],"systematic":[66],"study":[67],"comparing":[68],"efficacy":[70],"suitability":[72],"for":[73,81,164,225],"functional":[74],"studies":[75,226],"various":[78],"available":[79],"protocols":[80],"isolation":[83,180,247],"these":[85],"cells":[87],"not":[89],"been":[90],"performed.":[91],"Here,":[92],"we":[93,209],"compare":[94],"human":[95,166,228],"functionality":[97,155],"under":[98],"three":[99],"enrichment":[100,197],"protocols:":[101],"(i)":[102,123],"Ficoll":[103],"density":[104],"gradient":[105],"centrifugation,":[106],"(ii)":[107,127],"anti-CD15":[108],"antibody-conjugated":[109,131],"microbeads":[110,133,163],"(positive":[111],"selection),":[112,135],"(iii)":[114,136],"Polymorphoprep.":[115],"Primary":[116],"monocytes":[117,217],"were":[118,156],"isolated":[119],"in":[120,149,178,189,242],"parallel":[121],"using":[122],"anti-CD14":[124],"magnetic":[125,132,162],"microbeads,":[126],"non-monocyte":[128],"depletion":[129],"(negative":[134],"RosetteSep":[137],"antibody":[138],"cocktail,":[139],"(iv)":[141],"classical":[143],"adherence":[144],"protocol.":[145],"The":[146],"best":[147],"results":[148],"terms":[150],"purity":[152],"cell":[154,200,243],"obtained":[157],"with":[158,245],"positive":[159,212],"selection":[160,213],"both":[165],"granulocytes":[167,215],"monocytes.":[169],"Whereas":[170],"phagocytosis":[171],"Escherichia":[173],"coli":[174],"bacteria":[175],"was":[176,187],"identical":[177],"all":[179],"procedures":[181,198],"tested,":[182],"respiratory":[185],"burst":[186],"higher":[188],"positively":[190],"selected":[191],"cells.":[192],"In":[193,207],"addition,":[194],"different":[195,205,246],"affect":[199],"surface":[201],"receptor":[202],"expression":[203],"extents.":[206],"toto,":[208],"propose":[210],"adopted":[219],"procedure":[222],"choice":[224],"but":[233],"caution":[234],"investigators":[235],"aware":[238],"possible":[240],"alterations":[241],"phenotypes":[244],"procedures.":[248]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":5},{"year":2024,"cited_by_count":4},{"year":2023,"cited_by_count":4},{"year":2022,"cited_by_count":14},{"year":2021,"cited_by_count":7},{"year":2020,"cited_by_count":10},{"year":2019,"cited_by_count":11},{"year":2018,"cited_by_count":10},{"year":2017,"cited_by_count":7},{"year":2016,"cited_by_count":8},{"year":2015,"cited_by_count":8},{"year":2014,"cited_by_count":13},{"year":2013,"cited_by_count":3},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-16T09:59:22.235173","created_date":"2025-10-10T00:00:00"}