{"id":"https://openalex.org/W2163085455","doi":"https://doi.org/10.1128/iai.58.9.2792-2803.1990","title":"Cloning and characterization of T-cell-reactive protein antigens from Listeria monocytogenes","display_name":"Cloning and characterization of T-cell-reactive protein antigens from Listeria monocytogenes","publication_year":1990,"publication_date":"1990-09-01","ids":{"openalex":"https://openalex.org/W2163085455","doi":"https://doi.org/10.1128/iai.58.9.2792-2803.1990","mag":"2163085455","pmid":"https://pubmed.ncbi.nlm.nih.gov/2117570","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/313569"},"language":"en","primary_location":{"id":"doi:10.1128/iai.58.9.2792-2803.1990","doi":"https://doi.org/10.1128/iai.58.9.2792-2803.1990","is_oa":false,"landing_page_url":"https://doi.org/10.1128/iai.58.9.2792-2803.1990","pdf_url":null,"source":{"id":"https://openalex.org/S128227336","display_name":"Infection and Immunity","issn_l":"0019-9567","issn":["0019-9567","1070-6313","1098-5522"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Infection and Immunity","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://europepmc.org/articles/PMC313569","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5090455459","display_name":"I A Beattie","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I150468666","display_name":"Emory University","ror":"https://ror.org/03czfpz43","country_code":"US","type":"education","lineage":["https://openalex.org/I150468666"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"I A Beattie","raw_affiliation_strings":["Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30322"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30322","institution_ids":["https://openalex.org/I150468666"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111765019","display_name":"B. Swaminathan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I150468666","display_name":"Emory University","ror":"https://ror.org/03czfpz43","country_code":"US","type":"education","lineage":["https://openalex.org/I150468666"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"B Swaminathan","raw_affiliation_strings":["Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30322"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30322","institution_ids":["https://openalex.org/I150468666"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5076105089","display_name":"H. Ziegler","orcid":"https://orcid.org/0000-0003-2207-197X","observed_orcids":["https://orcid.org/0000-0003-2207-197X"]},"institutions":[{"id":"https://openalex.org/I150468666","display_name":"Emory University","ror":"https://ror.org/03czfpz43","country_code":"US","type":"education","lineage":["https://openalex.org/I150468666"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"H K Ziegler","raw_affiliation_strings":["Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30322"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30322","institution_ids":["https://openalex.org/I150468666"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I150468666"],"apc_list":null,"apc_paid":null,"fwci":1.3745,"has_fulltext":false,"cited_by_count":24,"citation_normalized_percentile":{"value":0.82138391,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":95},"biblio":{"volume":"58","issue":"9","first_page":"2792","last_page":"2803"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T13410","display_name":"Immune responses and vaccinations","score":0.3472000062465668,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T13410","display_name":"Immune responses and vaccinations","score":0.3472000062465668,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10580","display_name":"Immunotherapy and Immune Responses","score":0.25929999351501465,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10900","display_name":"Listeria monocytogenes in Food Safety","score":0.10130000114440918,"subfield":{"id":"https://openalex.org/subfields/1106","display_name":"Food Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/listeria-monocytogenes","display_name":"Listeria monocytogenes","score":1.0},{"id":"https://openalex.org/keywords/t-cell-antigens","display_name":"T cell antigens","score":0.8090000152587891},{"id":"https://openalex.org/keywords/listeriolysin-o","display_name":"listeriolysin O","score":0.9739999771118164},{"id":"https://openalex.org/keywords/t-cell-mediated-immunity","display_name":"T cell immunity","score":0.675000011920929},{"id":"https://openalex.org/keywords/antigen-presentation","display_name":"antigen presentation","score":0.3319999873638153},{"id":"https://openalex.org/keywords/macrophages","display_name":"macrophages","score":0.8190000057220459},{"id":"https://openalex.org/keywords/interleukin-2","display_name":"interleukin-2","score":0.8600000143051147},{"id":"https://openalex.org/keywords/recombinant-antigen-expression","display_name":"recombinant antigen expression","score":0.6800000071525574},{"id":"https://openalex.org/keywords/bacterial-virulence-factors","display_name":"bacterial virulence factors","score":0.7540000081062317},{"id":"https://openalex.org/keywords/bacterial-surface-proteins","display_name":"bacterial surface proteins","score":0.550000011920929}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8237398862838745},{"id":"https://openalex.org/C147483822","wikidata":"https://www.wikidata.org/wiki/Q103537","display_name":"Antigen","level":2,"score":0.7539840936660767},{"id":"https://openalex.org/C2781350384","wikidata":"https://www.wikidata.org/wiki/Q292015","display_name":"Listeria monocytogenes","level":3,"score":0.6492433547973633},{"id":"https://openalex.org/C956191","wikidata":"https://www.wikidata.org/wiki/Q930144","display_name":"Polyclonal antibodies","level":3,"score":0.5495990514755249},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.5107476711273193},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4813695549964905},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.45692041516304016},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.44732850790023804},{"id":"https://openalex.org/C2781275251","wikidata":"https://www.wikidata.org/wiki/Q17146593","display_name":"Listeriolysin O","level":5,"score":0.4359561800956726},{"id":"https://openalex.org/C8891405","wikidata":"https://www.wikidata.org/wiki/Q1059","display_name":"Immune system","level":2,"score":0.43097084760665894},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.3375580906867981},{"id":"https://openalex.org/C2778682378","wikidata":"https://www.wikidata.org/wiki/Q746209","display_name":"Listeria","level":4,"score":0.2045970857143402},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.13521820306777954},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.10665285587310791},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.07405215501785278},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000907","descriptor_name":"Antibodies, Bacterial","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D000938","descriptor_name":"Antigen-Presenting Cells","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D000942","descriptor_name":"Antigens, Bacterial","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D000942","descriptor_name":"Antigens, Bacterial","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D001427","descriptor_name":"Bacterial Toxins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004269","descriptor_name":"DNA, Bacterial","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006360","descriptor_name":"Heat-Shock Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D006360","descriptor_name":"Heat-Shock Proteins","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D006460","descriptor_name":"Hemolysin Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D006460","descriptor_name":"Hemolysin Proteins","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D008089","descriptor_name":"Listeria monocytogenes","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D008213","descriptor_name":"Lymphocyte Activation","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D008264","descriptor_name":"Macrophages","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D008809","descriptor_name":"Mice, Inbred C3H","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D013601","descriptor_name":"T-Lymphocytes","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1128/iai.58.9.2792-2803.1990","doi":"https://doi.org/10.1128/iai.58.9.2792-2803.1990","is_oa":false,"landing_page_url":"https://doi.org/10.1128/iai.58.9.2792-2803.1990","pdf_url":null,"source":{"id":"https://openalex.org/S128227336","display_name":"Infection and Immunity","issn_l":"0019-9567","issn":["0019-9567","1070-6313","1098-5522"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Infection and Immunity","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:1462608","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/articles/PMC313569","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:313569","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/313569","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Infect Immun","raw_type":"Text"},{"id":"pmid:2117570","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2117570","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Infection and immunity","raw_type":"Journal Article"}],"best_oa_location":{"id":"pmh:oai:europepmc.org:1462608","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/articles/PMC313569","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},"sustainable_development_goals":[{"score":0.8048999905586243,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":39,"referenced_works":["https://openalex.org/W1492002550","https://openalex.org/W1498972346","https://openalex.org/W1511050873","https://openalex.org/W1514908334","https://openalex.org/W1590451279","https://openalex.org/W1608651908","https://openalex.org/W1875673442","https://openalex.org/W1892225881","https://openalex.org/W1898510548","https://openalex.org/W1909750055","https://openalex.org/W2010168640","https://openalex.org/W2023871230","https://openalex.org/W2030522563","https://openalex.org/W2032413102","https://openalex.org/W2033440580","https://openalex.org/W2036663468","https://openalex.org/W2044878758","https://openalex.org/W2063988753","https://openalex.org/W2074122548","https://openalex.org/W2081807824","https://openalex.org/W2100558272","https://openalex.org/W2100837269","https://openalex.org/W2111817213","https://openalex.org/W2127471968","https://openalex.org/W2130146160","https://openalex.org/W2135705262","https://openalex.org/W2137571507","https://openalex.org/W2141486991","https://openalex.org/W2142171826","https://openalex.org/W2143735644","https://openalex.org/W2144014651","https://openalex.org/W2144634347","https://openalex.org/W2147171214","https://openalex.org/W2147425141","https://openalex.org/W2155002424","https://openalex.org/W2236777032","https://openalex.org/W2341414227","https://openalex.org/W2409088848","https://openalex.org/W2766276685"],"related_works":["https://openalex.org/W1512854437","https://openalex.org/W2390971865","https://openalex.org/W2163904966","https://openalex.org/W2104231805","https://openalex.org/W1776002807","https://openalex.org/W2412179593","https://openalex.org/W2131717322","https://openalex.org/W7100729647","https://openalex.org/W2281867130","https://openalex.org/W2098318598"],"abstract_inverted_index":{"To":[0],"explore":[1],"the":[2,6,23,40,49,98,109,144,180,221,238,247,255],"molecular":[3],"basis":[4],"of":[5,61,207,220,224,231,254],"T-cell-mediated":[7],"immune":[8,259],"response":[9,182,240],"to":[10,36,97,107,241,261],"Listeria":[11],"monocytogenes,":[12],"we":[13,160],"cloned":[14],"and":[15,26,48,71,86,141,147,153,191,194,234],"expressed":[16,142],"listerial":[17,167],"antigens":[18,168,256],"in":[19,69,76,143,175,179,218,237,258],"Escherichia":[20],"coli":[21],"using":[22],"lambda-ZAP":[24],"bacteriophage":[25],"Bluescript":[27,145],"plasmid":[28,146],"vectors.":[29],"A":[30,59],"two-stage":[31],"screening":[32,158],"strategy":[33],"was":[34,52,67],"implemented":[35],"identify":[37],"T-cell-reactive":[38],"antigens;":[39],"first":[41,248],"stage":[42,51],"involved":[43],"antibodies":[44],"or":[45],"oligonucleotide":[46,94],"probes":[47,95],"second":[50],"based":[53],"on":[54],"assays":[55],"for":[56,101,120,150],"T-cell":[57,121,181,239],"activation.":[58],"library":[60],"genomic":[62],"DNA":[63,111],"from":[64,133],"L.":[65,88,134,171,186,242,262],"monocytogenes":[66,84,187],"generated":[68],"lambda-ZAP,":[70],"then":[72],"antigens,":[73],"were":[74,105,118,139],"detected":[75],"infected":[77],"cells":[78,131,174],"with":[79,185],"a":[80,227,251],"polyclonal":[81,129],"rabbit":[82],"anti-L.":[83],"antiserum":[85],"an":[87],"monocytogenes-specific":[89],"monoclonal":[90],"antibody.":[91],"Also,":[92],"synthetic":[93],"corresponding":[96],"structural":[99],"gene":[100],"listeriolysin":[102],"O":[103],"(LLO)":[104],"used":[106],"screen":[108],"recombinant":[110,166,208],"library.":[112],"In":[113],"each":[114],"case,":[115],"positive":[116],"isolates":[117],"evaluated":[119],"antigenicity":[122],"by":[123,128],"measuring":[124],"antigen-induced":[125],"interleukin-2":[126],"production":[127],"T":[130,173],"taken":[132],"monocytogenes-immune":[135,172],"mice.":[136],"Phage":[137],"clones":[138,164],"subcloned":[140],"tested":[148],"further":[149],"antigenic":[151],"activity":[152],"LLO":[154,209,225],"expression.":[155],"Using":[156],"this":[157],"strategy,":[159],"successfully":[161],"identified":[162],"bacterial":[163,198],"producing":[165],"which":[169],"activate":[170],"vitro.":[176],"Antigens":[177],"operative":[178],"during":[183],"infection":[184],"include":[188],"LLO,":[189],"62-":[190],"39-kilodalton":[192],"proteins,":[193],"other":[195],"poorly":[196],"defined":[197],"surface":[199],"components.":[200],"We":[201],"also":[202],"found":[203],"that":[204],"high":[205],"concentrations":[206],"inhibited":[210],"macrophage-mediated":[211],"antigen":[212,232,236],"presentation.":[213],"These":[214,244],"results":[215],"are":[216],"discussed":[217],"terms":[219],"multiple":[222],"functions":[223],"as":[226],"virulence":[228],"factor,":[229],"inhibitor":[230],"presentation,":[233],"potent":[235],"monocytogenes.":[243,263],"studies":[245],"represent":[246],"step":[249],"toward":[250],"genetic":[252],"definition":[253],"recognized":[257],"defense":[260]},"counts_by_year":[{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":2}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}