{"id":"https://openalex.org/W2162995688","doi":"https://doi.org/10.1128/iai.00473-10","title":"Developmental Expression of a Trypanosoma cruzi Phosphoinositide-Specific Phospholipase C in Amastigotes and Stimulation of Host Phosphoinositide Hydrolysis","display_name":"Developmental Expression of a Trypanosoma cruzi Phosphoinositide-Specific Phospholipase C in Amastigotes and Stimulation of Host Phosphoinositide Hydrolysis","publication_year":2010,"publication_date":"2010-07-20","ids":{"openalex":"https://openalex.org/W2162995688","doi":"https://doi.org/10.1128/iai.00473-10","mag":"2162995688","pmid":"https://pubmed.ncbi.nlm.nih.gov/20643853","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/2950344"},"language":"en","primary_location":{"id":"doi:10.1128/iai.00473-10","doi":"https://doi.org/10.1128/iai.00473-10","is_oa":false,"landing_page_url":"https://doi.org/10.1128/iai.00473-10","pdf_url":null,"source":{"id":"https://openalex.org/S128227336","display_name":"Infection and Immunity","issn_l":"0019-9567","issn":["0019-9567","1070-6313","1098-5522"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Infection and Immunity","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2950344","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5032394490","display_name":"Vicente de Paulo Martins","orcid":"https://orcid.org/0000-0001-7611-861X","observed_orcids":["https://orcid.org/0000-0001-7611-861X"]},"institutions":[{"id":"https://openalex.org/I165733156","display_name":"University of Georgia","ror":"https://ror.org/00te3t702","country_code":"US","type":"education","lineage":["https://openalex.org/I165733156"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Vicente de Paulo Martins","raw_affiliation_strings":["Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, Georgia 30602"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, Georgia 30602","institution_ids":["https://openalex.org/I165733156"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5019890987","display_name":"Melina Galizzi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I165733156","display_name":"University of Georgia","ror":"https://ror.org/00te3t702","country_code":"US","type":"education","lineage":["https://openalex.org/I165733156"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Melina Galizzi","raw_affiliation_strings":["Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, Georgia 30602"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, Georgia 30602","institution_ids":["https://openalex.org/I165733156"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5031602023","display_name":"Mar\u0131\u0301a Laura Salto","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I165733156","display_name":"University of Georgia","ror":"https://ror.org/00te3t702","country_code":"US","type":"education","lineage":["https://openalex.org/I165733156"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Maria Laura Salto","raw_affiliation_strings":["Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, Georgia 30602"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, Georgia 30602","institution_ids":["https://openalex.org/I165733156"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5000842323","display_name":"Roberto Docampo","orcid":"https://orcid.org/0000-0003-4229-8784","observed_orcids":["https://orcid.org/0000-0003-4229-8784"]},"institutions":[{"id":"https://openalex.org/I165733156","display_name":"University of Georgia","ror":"https://ror.org/00te3t702","country_code":"US","type":"education","lineage":["https://openalex.org/I165733156"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Roberto Docampo","raw_affiliation_strings":["Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, Georgia 30602"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, Georgia 30602","institution_ids":["https://openalex.org/I165733156"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5103343731","display_name":"Silvia N.J. Moreno","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I165733156","display_name":"University of Georgia","ror":"https://ror.org/00te3t702","country_code":"US","type":"education","lineage":["https://openalex.org/I165733156"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Silvia N. J. Moreno","raw_affiliation_strings":["Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, Georgia 30602"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, Georgia 30602","institution_ids":["https://openalex.org/I165733156"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5103343731"],"corresponding_institution_ids":["https://openalex.org/I165733156"],"apc_list":null,"apc_paid":null,"fwci":1.8821,"has_fulltext":false,"cited_by_count":22,"citation_normalized_percentile":{"value":0.84857642,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"78","issue":"10","first_page":"4206","last_page":"4212"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10890","display_name":"Trypanosoma species research and implications","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10890","display_name":"Trypanosoma species research and implications","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10823","display_name":"Research on Leishmaniasis Studies","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10617","display_name":"Cellular transport and secretion","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/trypanosoma-cruzi","display_name":"Trypanosoma cruzi","score":1.0},{"id":"https://openalex.org/keywords/phosphatidylinositol-specific-phospholipase-c","display_name":"phosphatidylinositol-specific phospholipase C","score":0.9490000009536743},{"id":"https://openalex.org/keywords/amastigotes","display_name":"amastigotes","score":0.9259999990463257},{"id":"https://openalex.org/keywords/trypomastigotes","display_name":"trypomastigotes","score":0.8889999985694885},{"id":"https://openalex.org/keywords/developmental-regulation","display_name":"developmental regulation","score":0.8889999985694885},{"id":"https://openalex.org/keywords/inositol-1-4-5-trisphosphate","display_name":"inositol 1,4,5-trisphosphate","score":0.9919999837875366},{"id":"https://openalex.org/keywords/host-parasite-interactions","display_name":"host-parasite interactions","score":0.796999990940094},{"id":"https://openalex.org/keywords/parasite-differentiation","display_name":"parasite differentiation","score":0.4050000011920929},{"id":"https://openalex.org/keywords/calcium-signaling","display_name":"calcium signaling","score":0.7419999837875366},{"id":"https://openalex.org/keywords/cytoskeletal-changes","display_name":"cytoskeletal changes","score":0.7160000205039978}],"concepts":[{"id":"https://openalex.org/C98319499","wikidata":"https://www.wikidata.org/wiki/Q455445","display_name":"Amastigote","level":4,"score":0.9014643430709839},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.826204776763916},{"id":"https://openalex.org/C2778597717","wikidata":"https://www.wikidata.org/wiki/Q420248","display_name":"Phospholipase C","level":3,"score":0.7707333564758301},{"id":"https://openalex.org/C2777625466","wikidata":"https://www.wikidata.org/wiki/Q150162","display_name":"Trypanosoma cruzi","level":3,"score":0.7484097480773926},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.7146797776222229},{"id":"https://openalex.org/C2780610907","wikidata":"https://www.wikidata.org/wiki/Q2273248","display_name":"Phosphatidylinositol","level":3,"score":0.7141516804695129},{"id":"https://openalex.org/C2777427919","wikidata":"https://www.wikidata.org/wiki/Q407997","display_name":"Inositol","level":3,"score":0.6566808223724365},{"id":"https://openalex.org/C2777853560","wikidata":"https://www.wikidata.org/wiki/Q3083814","display_name":"Phosphatidylinositol 4,5-bisphosphate","level":4,"score":0.5293097496032715},{"id":"https://openalex.org/C28406088","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Extracellular","level":2,"score":0.4462837874889374},{"id":"https://openalex.org/C78297661","wikidata":"https://www.wikidata.org/wiki/Q10760862","display_name":"Phosphoinositide phospholipase C","level":4,"score":0.44076448678970337},{"id":"https://openalex.org/C62478195","wikidata":"https://www.wikidata.org/wiki/Q828130","display_name":"Signal transduction","level":2,"score":0.4032733142375946},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.29941415786743164},{"id":"https://openalex.org/C71928629","wikidata":"https://www.wikidata.org/wiki/Q804206","display_name":"Parasite hosting","level":2,"score":0.14069640636444092},{"id":"https://openalex.org/C2781092759","wikidata":"https://www.wikidata.org/wiki/Q524818","display_name":"Leishmania","level":3,"score":0.08559831976890564},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.07877972722053528},{"id":"https://openalex.org/C136764020","wikidata":"https://www.wikidata.org/wiki/Q466","display_name":"World Wide Web","level":1,"score":0.0},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002462","descriptor_name":"Cell Membrane","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D006790","descriptor_name":"Host-Parasite Interactions","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006868","descriptor_name":"Hydrolysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007295","descriptor_name":"Inositol Phosphates","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D014349","descriptor_name":"Trypanosoma cruzi","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D015398","descriptor_name":"Signal Transduction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015971","descriptor_name":"Gene Expression Regulation, Enzymologic","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D018507","descriptor_name":"Gene Expression Regulation, Developmental","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D019269","descriptor_name":"Phosphatidylinositol 4,5-Diphosphate","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D032446","descriptor_name":"Myoblasts","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D032446","descriptor_name":"Myoblasts","qualifier_ui":"Q000469","qualifier_name":"parasitology","is_major_topic":false},{"descriptor_ui":"D054801","descriptor_name":"Phosphoinositide Phospholipase C","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D054801","descriptor_name":"Phosphoinositide Phospholipase C","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1128/iai.00473-10","doi":"https://doi.org/10.1128/iai.00473-10","is_oa":false,"landing_page_url":"https://doi.org/10.1128/iai.00473-10","pdf_url":null,"source":{"id":"https://openalex.org/S128227336","display_name":"Infection and Immunity","issn_l":"0019-9567","issn":["0019-9567","1070-6313","1098-5522"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Infection and Immunity","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:2950344","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2950344","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Infect Immun","raw_type":"Text"},{"id":"pmid:20643853","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/20643853","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Infection and immunity","raw_type":"Journal Article"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:2950344","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2950344","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Infect Immun","raw_type":"Text"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.46810001134872437}],"study_designs":[],"awards":[{"id":"https://openalex.org/G1400196626","display_name":"Novel phospholipases C in trypanosomatids","funder_award_id":"R01AI058297","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":27,"referenced_works":["https://openalex.org/W113166253","https://openalex.org/W1554222608","https://openalex.org/W1574660002","https://openalex.org/W1975727221","https://openalex.org/W1981617663","https://openalex.org/W1984020576","https://openalex.org/W1993282564","https://openalex.org/W2008086515","https://openalex.org/W2010485774","https://openalex.org/W2023847294","https://openalex.org/W2025218591","https://openalex.org/W2028263525","https://openalex.org/W2038736974","https://openalex.org/W2042834905","https://openalex.org/W2072375808","https://openalex.org/W2092944630","https://openalex.org/W2097939257","https://openalex.org/W2101386476","https://openalex.org/W2109799285","https://openalex.org/W2112451562","https://openalex.org/W2129145334","https://openalex.org/W2134283668","https://openalex.org/W2136189765","https://openalex.org/W2139126967","https://openalex.org/W2161344955","https://openalex.org/W2165564070","https://openalex.org/W2401104795"],"related_works":["https://openalex.org/W2025218591","https://openalex.org/W2112451562","https://openalex.org/W2615737565","https://openalex.org/W2593173985","https://openalex.org/W7100146542","https://openalex.org/W2023847294","https://openalex.org/W2070580865","https://openalex.org/W2158889972","https://openalex.org/W2093334092","https://openalex.org/W3025620434"],"abstract_inverted_index":{"Phosphoinositide":[0],"phospholipase":[1],"C":[2],"(PI-PLC)":[3],"plays":[4],"an":[5,95],"essential":[6],"role":[7],"in":[8,20,44,88,97],"cell":[9,91],"signaling.":[10],"A":[11],"unique":[12],"Trypanosoma":[13],"cruzi":[14],"PI-PLC":[15,108],"(TcPI-PLC)":[16],"is":[17,41,109],"lipid":[18],"modified":[19],"its":[21,101],"N":[22],"terminus":[23],"and":[24,46,69,93,125,135,155,161],"localizes":[25],"to":[26,158,162],"the":[27,31,54,60,70,89,98,112,121,145,149,153,156,170],"outer":[28,146],"surface":[29,51,122,147],"of":[30,34,50,57,65,74,80,100,118,123,142,148,152,169],"plasma":[32,150],"membrane":[33,92,151],"amastigotes.":[35],"We":[36],"show":[37],"here":[38],"that":[39],"TcPI-PLC":[40,81,119,143],"developmentally":[42],"regulated":[43],"amastigotes":[45,68,75,124],"shows":[47],"two":[48],"peaks":[49],"expression":[52,79,117],"during":[53],"developmental":[55],"cycle":[56],"T.":[58],"cruzi,":[59],"first":[61],"immediately":[62],"after":[63],"differentiation":[64,73],"trypomastigotes":[66],"into":[67,76,111],"second":[71],"before":[72],"trypomastigotes.":[77],"Surface":[78],"coincides":[82],"with":[83,94,129],"phosphatidylinositol":[84],"4,5-bisphosphate":[85],"(PIP(2))":[86],"depletion":[87,127],"host":[90,130,164],"increase":[96],"levels":[99],"product,":[102],"inositol":[103],"1,4,5-trisphosphate.":[104],"During":[105],"extracellular":[106],"differentiation,":[107],"secreted":[110,160],"incubation":[113],"medium.":[114],"Maximal":[115],"early":[116],"on":[120,144],"PIP(2)":[126],"coincide":[128],"cytoskeletal":[131],"changes,":[132],"Ca(2+)":[133],"signaling,":[134],"transcriptional":[136],"responses":[137],"described":[138],"previously.":[139],"The":[140],"presence":[141],"parasite":[154],"capacity":[157],"be":[159],"alter":[163],"phospholipids":[165],"are":[166],"novel":[167],"mechanisms":[168],"host-parasite":[171],"interaction.":[172]},"counts_by_year":[{"year":2025,"cited_by_count":2},{"year":2021,"cited_by_count":5},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":1},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":1},{"year":2013,"cited_by_count":4},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2016-06-24T00:00:00"}