{"id":"https://openalex.org/W2162575358","doi":"https://doi.org/10.1021/bp000057p","title":"Influence of Baculovirus\u2010Host Cell Interactions on Complex N\u2010Linked Glycosylation of a Recombinant Human Protein","display_name":"Influence of Baculovirus\u2010Host Cell Interactions on Complex N\u2010Linked Glycosylation of a Recombinant Human Protein","publication_year":2000,"publication_date":"2000-01-01","ids":{"openalex":"https://openalex.org/W2162575358","doi":"https://doi.org/10.1021/bp000057p","mag":"2162575358","pmid":"https://pubmed.ncbi.nlm.nih.gov/10933841"},"language":"en","primary_location":{"id":"doi:10.1021/bp000057p","doi":"https://doi.org/10.1021/bp000057p","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bp000057p","pdf_url":null,"source":{"id":"https://openalex.org/S186451451","display_name":"Biotechnology Progress","issn_l":"1520-6033","issn":["1520-6033","8756-7938"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology Progress","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5027594012","display_name":"Lokesh Joshi","orcid":"https://orcid.org/0000-0002-3612-0747","observed_orcids":["https://orcid.org/0000-0002-3612-0747"]},"institutions":[{"id":"https://openalex.org/I205783295","display_name":"Cornell University","ror":"https://ror.org/05bnh6r87","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295"]},{"id":"https://openalex.org/I4405258788","display_name":"Boyce Thompson Institute","ror":"https://ror.org/016tr2327","country_code":"US","type":"facility","lineage":["https://openalex.org/I4405258788"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Lokesh Joshi","raw_affiliation_strings":["Boyce Thompson Institute at Cornell University, Tower Road, Ithaca, New York 14853, USA","Boyce Thompson Institute at Cornell University, Tower Road, Ithaca, New York 14853"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Boyce Thompson Institute at Cornell University, Tower Road, Ithaca, New York 14853, USA","institution_ids":["https://openalex.org/I205783295","https://openalex.org/I4405258788"]},{"raw_affiliation_string":"Boyce Thompson Institute at Cornell University, Tower Road, Ithaca, New York 14853","institution_ids":["https://openalex.org/I205783295","https://openalex.org/I4405258788"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5005307444","display_name":"Thomas R. Davis","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I209244073","display_name":"Pioneer Hi-Bred","ror":"https://ror.org/02nn4s922","country_code":"US","type":"company","lineage":["https://openalex.org/I209244073"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Thomas R. Davis","raw_affiliation_strings":["Pioneer Hybrid International Inc., 7300 Northwest 62nd Avenue, Johnston, Iowa 50131"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pioneer Hybrid International Inc., 7300 Northwest 62nd Avenue, Johnston, Iowa 50131","institution_ids":["https://openalex.org/I209244073"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5008880145","display_name":"Taj S. Mattu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I40120149","display_name":"University of Oxford","ror":"https://ror.org/052gg0110","country_code":"GB","type":"education","lineage":["https://openalex.org/I40120149"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Taj S. Mattu","raw_affiliation_strings":["The Glycobiology Institute, Department of Biochemistry, Oxford University, South Parks Road, Oxford OX1 3QU, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Glycobiology Institute, Department of Biochemistry, Oxford University, South Parks Road, Oxford OX1 3QU, United Kingdom","institution_ids":["https://openalex.org/I40120149"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5082473050","display_name":"Pauline Mary Rudd","orcid":"https://orcid.org/0000-0003-3880-0532","observed_orcids":["https://orcid.org/0000-0003-3880-0532"]},"institutions":[{"id":"https://openalex.org/I40120149","display_name":"University of Oxford","ror":"https://ror.org/052gg0110","country_code":"GB","type":"education","lineage":["https://openalex.org/I40120149"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Pauline M. Rudd","raw_affiliation_strings":["The Glycobiology Institute, Department of Biochemistry, Oxford University, South Parks Road, Oxford OX1 3QU, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Glycobiology Institute, Department of Biochemistry, Oxford University, South Parks Road, Oxford OX1 3QU, United Kingdom","institution_ids":["https://openalex.org/I40120149"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5062018516","display_name":"Raymond Allen Dwek","orcid":"https://orcid.org/0000-0001-9989-2245","observed_orcids":["https://orcid.org/0000-0001-9989-2245"]},"institutions":[{"id":"https://openalex.org/I40120149","display_name":"University of Oxford","ror":"https://ror.org/052gg0110","country_code":"GB","type":"education","lineage":["https://openalex.org/I40120149"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Raymond A. Dwek","raw_affiliation_strings":["The Glycobiology Institute, Department of Biochemistry, Oxford University, South Parks Road, Oxford OX1 3QU, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Glycobiology Institute, Department of Biochemistry, Oxford University, South Parks Road, Oxford OX1 3QU, United Kingdom","institution_ids":["https://openalex.org/I40120149"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5008392252","display_name":"Michael Louis Shuler","orcid":"https://orcid.org/0000-0002-4662-317X","observed_orcids":["https://orcid.org/0000-0002-4662-317X"]},"institutions":[{"id":"https://openalex.org/I205783295","display_name":"Cornell University","ror":"https://ror.org/05bnh6r87","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Michael L. Shuler","raw_affiliation_strings":["School of Chemical Engineering, Cornell University, 120 Olin Hall, Ithaca, New York 14853"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Chemical Engineering, Cornell University, 120 Olin Hall, Ithaca, New York 14853","institution_ids":["https://openalex.org/I205783295"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5109893700","display_name":"H. Alan Wood","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I205783295","display_name":"Cornell University","ror":"https://ror.org/05bnh6r87","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295"]},{"id":"https://openalex.org/I4405258788","display_name":"Boyce Thompson Institute","ror":"https://ror.org/016tr2327","country_code":"US","type":"facility","lineage":["https://openalex.org/I4405258788"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"H. Alan Wood","raw_affiliation_strings":["Boyce Thompson Institute at Cornell University, Tower Road, Ithaca, New York 14853"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Boyce Thompson Institute at Cornell University, Tower Road, Ithaca, New York 14853","institution_ids":["https://openalex.org/I205783295","https://openalex.org/I4405258788"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5109893700"],"corresponding_institution_ids":["https://openalex.org/I205783295","https://openalex.org/I4405258788"],"apc_list":{"value":4160,"currency":"USD","value_usd":4160},"apc_paid":null,"fwci":0.9647,"has_fulltext":false,"cited_by_count":34,"citation_normalized_percentile":{"value":0.74041967,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":"16","issue":"4","first_page":"650","last_page":"656"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10602","display_name":"Glycosylation and Glycoproteins Research","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12856","display_name":"Transgenic Plants and Applications","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/n-glycosylation","display_name":"N-glycosylation","score":0.9549999833106995},{"id":"https://openalex.org/keywords/baculovirus-expression-system","display_name":"baculovirus expression system","score":0.8410000205039978},{"id":"https://openalex.org/keywords/secreted-alkaline-phosphatase","display_name":"secreted alkaline phosphatase","score":0.8949999809265137},{"id":"https://openalex.org/keywords/insect-cell-culture","display_name":"insect cell culture","score":0.7300000190734863},{"id":"https://openalex.org/keywords/autographa-californica-multiple-nucleopolyhedrovirus","display_name":"Autographa californica nuclear polyhedrosis virus","score":0.9120000004768372},{"id":"https://openalex.org/keywords/sialic-acid","display_name":"sialic acid","score":0.7919999957084656},{"id":"https://openalex.org/keywords/serum-free-medium","display_name":"serum-free medium","score":0.5619999766349792},{"id":"https://openalex.org/keywords/sf9-cells","display_name":"Sf9 cells","score":0.703000009059906},{"id":"https://openalex.org/keywords/recombinant-human-proteins","display_name":"recombinant human proteins","score":0.6100000143051147},{"id":"https://openalex.org/keywords/glycoforms","display_name":"glycoforms","score":0.7059999704360962}],"concepts":[{"id":"https://openalex.org/C2779341487","wikidata":"https://www.wikidata.org/wiki/Q1375305","display_name":"Trichoplusia","level":4,"score":0.8384343385696411},{"id":"https://openalex.org/C103878061","wikidata":"https://www.wikidata.org/wiki/Q28840674","display_name":"Sf9","level":5,"score":0.8278406858444214},{"id":"https://openalex.org/C2780199244","wikidata":"https://www.wikidata.org/wiki/Q4826309","display_name":"Autographa californica","level":5,"score":0.8146102428436279},{"id":"https://openalex.org/C2777313579","wikidata":"https://www.wikidata.org/wiki/Q898365","display_name":"Glycosylation","level":2,"score":0.7723644971847534},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7487326264381409},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.7178879976272583},{"id":"https://openalex.org/C2777212814","wikidata":"https://www.wikidata.org/wiki/Q763221","display_name":"Baculoviridae","level":5,"score":0.7036818861961365},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.6660088300704956},{"id":"https://openalex.org/C206212055","wikidata":"https://www.wikidata.org/wiki/Q2553138","display_name":"Glycan","level":3,"score":0.5520223379135132},{"id":"https://openalex.org/C2780115458","wikidata":"https://www.wikidata.org/wiki/Q419311","display_name":"Sialic acid","level":2,"score":0.5225315093994141},{"id":"https://openalex.org/C30324644","wikidata":"https://www.wikidata.org/wiki/Q3334151","display_name":"N-linked glycosylation","level":4,"score":0.4728156328201294},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.42051175236701965},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4057605266571045},{"id":"https://openalex.org/C2777773057","wikidata":"https://www.wikidata.org/wiki/Q2492887","display_name":"Spodoptera","level":4,"score":0.3854982256889343},{"id":"https://openalex.org/C108625454","wikidata":"https://www.wikidata.org/wiki/Q187126","display_name":"Glycoprotein","level":2,"score":0.37841469049453735},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.22955578565597534},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0682956874370575},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.0},{"id":"https://openalex.org/C173758957","wikidata":"https://www.wikidata.org/wiki/Q129270","display_name":"Larva","level":2,"score":0.0},{"id":"https://openalex.org/C2778350275","wikidata":"https://www.wikidata.org/wiki/Q459180","display_name":"Noctuidae","level":3,"score":0.0}],"mesh":[{"descriptor_ui":"D000469","descriptor_name":"Alkaline Phosphatase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000469","descriptor_name":"Alkaline Phosphatase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000469","descriptor_name":"Alkaline Phosphatase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002851","descriptor_name":"Chromatography, High Pressure Liquid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002851","descriptor_name":"Chromatography, High Pressure Liquid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002851","descriptor_name":"Chromatography, High Pressure Liquid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006031","descriptor_name":"Glycosylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006031","descriptor_name":"Glycosylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006031","descriptor_name":"Glycosylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011134","descriptor_name":"Polysaccharides","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011134","descriptor_name":"Polysaccharides","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011134","descriptor_name":"Polysaccharides","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D016367","descriptor_name":"Baculoviridae","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D016367","descriptor_name":"Baculoviridae","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D016367","descriptor_name":"Baculoviridae","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D016367","descriptor_name":"Baculoviridae","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D016367","descriptor_name":"Baculoviridae","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D016367","descriptor_name":"Baculoviridae","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D016895","descriptor_name":"Culture Media, Serum-Free","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016895","descriptor_name":"Culture Media, Serum-Free","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016895","descriptor_name":"Culture Media, Serum-Free","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018411","descriptor_name":"Spodoptera","qualifier_ui":"Q000821","qualifier_name":"virology","is_major_topic":false},{"descriptor_ui":"D018411","descriptor_name":"Spodoptera","qualifier_ui":"Q000821","qualifier_name":"virology","is_major_topic":false},{"descriptor_ui":"D018411","descriptor_name":"Spodoptera","qualifier_ui":"Q000821","qualifier_name":"virology","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1021/bp000057p","doi":"https://doi.org/10.1021/bp000057p","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bp000057p","pdf_url":null,"source":{"id":"https://openalex.org/S186451451","display_name":"Biotechnology Progress","issn_l":"1520-6033","issn":["1520-6033","8756-7938"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology Progress","raw_type":"journal-article"},{"id":"pmid:10933841","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10933841","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology progress","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"sustainable_development_goals_aurora":[{"display_name":"Life below water","score":0.8899999856948853,"id":"https://metadata.un.org/sdg/14"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":32,"referenced_works":["https://openalex.org/W257561636","https://openalex.org/W600006823","https://openalex.org/W606298846","https://openalex.org/W1549985235","https://openalex.org/W1975351631","https://openalex.org/W1986005654","https://openalex.org/W1986859398","https://openalex.org/W1991139188","https://openalex.org/W2002778736","https://openalex.org/W2011006472","https://openalex.org/W2011452349","https://openalex.org/W2016592187","https://openalex.org/W2023234589","https://openalex.org/W2040867334","https://openalex.org/W2043286541","https://openalex.org/W2044205177","https://openalex.org/W2055507506","https://openalex.org/W2067744893","https://openalex.org/W2074015027","https://openalex.org/W2084324934","https://openalex.org/W2086263410","https://openalex.org/W2094678638","https://openalex.org/W2094945468","https://openalex.org/W2112969703","https://openalex.org/W2116508878","https://openalex.org/W2129232972","https://openalex.org/W2141670942","https://openalex.org/W2143678448","https://openalex.org/W2156826212","https://openalex.org/W2281202791","https://openalex.org/W2414045509","https://openalex.org/W2903005696"],"related_works":["https://openalex.org/W7095722735","https://openalex.org/W1513147618","https://openalex.org/W2018457584","https://openalex.org/W2145835857","https://openalex.org/W2141670942","https://openalex.org/W1986005654","https://openalex.org/W2139312302","https://openalex.org/W1985140325","https://openalex.org/W2325902452","https://openalex.org/W65492278"],"abstract_inverted_index":{"The":[0,168],"conditions":[1],"required":[2],"for":[3],"mammalian-type":[4],"complex":[5,76,110,166],"N-linked":[6,26,203],"glycosylation":[7,27,188],"of":[8,28,104,138,202,208],"human":[9,29],"proteins":[10],"produced":[11,150],"in":[12,60,66,127,131,151,155,190,194,197],"insect":[13,41,191],"cells":[14,64,129,153],"with":[15,37,84,158],"the":[16,85,98,105,139,178,187,206,213],"baculovirus":[17,39,86,172],"expression":[18,87],"vector":[19,88],"system":[20],"were":[21,35,107],"investigated.":[22],"Marked":[23],"alterations":[24,196],"to":[25,57,79,97,124,212,221],"placental":[30],"secreted":[31],"alkaline":[32],"phosphatase":[33],"(SEAP)":[34],"observed":[36],"different":[38],"species,":[40],"cell":[42,45,175],"lines,":[43],"and":[44,74,164,177,199],"culture":[46,99],"media.":[47],"When":[48,114],"a":[49,81,115,183],"recombinant":[50,116],"Autographa":[51],"californica":[52],"nucleopolyhedrovirus":[53,119],"(AcMNPV)":[54],"was":[55,95,121,216],"used":[56,123],"produce":[58],"SEAP":[59,126,149,214],"Trichoplusia":[61],"ni":[62,118],"(Tn-4h)":[63],"cultured":[65,130],"serum-free":[67],"medium,":[68,100,133],"structural":[69],"analyses":[70],"indicated":[71],"<1%":[72,161],"hybrid":[73,108,162],"no":[75,165],"oligosaccharides":[77,106,163],"attached":[78],"SEAP,":[80],"typical":[82],"result":[83],"system.":[89],"However,":[90],"when":[91],"fetal":[92],"bovine":[93],"serum":[94],"added":[96],"48":[101],"+/-":[102,136],"4%":[103],"or":[109],"(but":[111],"asialylated)":[112],"glycans.":[113],"T.":[117],"(TnSNPV)":[120],"similarly":[122],"express":[125],"Tn-4h":[128],"serum-containing":[132,156],"only":[134],"24":[135],"3%":[137],"glycans":[140,215,224],"contained":[141,160],"terminal":[142],"N-acetylglucosamine":[143],"and/or":[144],"galactose":[145],"residues.":[146],"In":[147],"contrast,":[148],"Sf9":[152],"grown":[154],"medium":[157,180],"AcMNPV":[159],"oligosaccharides.":[167],"results":[169],"illustrate":[170],"that":[171],"type,":[173,176],"host":[174],"growth":[179],"all":[181],"have":[182],"strong":[184],"influence":[185],"on":[186],"pathway":[189],"cells,":[192],"resulting":[193],"significant":[195],"structures":[198],"relative":[200],"abundance":[201],"glycoforms.":[204],"Although":[205],"addition":[207],"sialic":[209],"acid":[210],"residues":[211],"not":[217],"detected,":[218],"possible":[219],"approaches":[220],"obtain":[222],"sialylated":[223],"are":[225],"discussed.":[226]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2022,"cited_by_count":2},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2014,"cited_by_count":1}],"updated_date":"2026-10-01T08:54:57.152042","created_date":"2016-06-24T00:00:00"}