{"id":"https://openalex.org/W2161663291","doi":"https://doi.org/10.1128/aem.65.5.1915-1918.1999","title":"Expression of an Aspergillus niger Phytase Gene ( phyA ) in Saccharomyces cerevisiae","display_name":"Expression of an Aspergillus niger Phytase Gene ( phyA ) in Saccharomyces cerevisiae","publication_year":1999,"publication_date":"1999-05-01","ids":{"openalex":"https://openalex.org/W2161663291","doi":"https://doi.org/10.1128/aem.65.5.1915-1918.1999","mag":"2161663291","pmid":"https://pubmed.ncbi.nlm.nih.gov/10223979","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/91276"},"language":"en","primary_location":{"id":"doi:10.1128/aem.65.5.1915-1918.1999","doi":"https://doi.org/10.1128/aem.65.5.1915-1918.1999","is_oa":true,"landing_page_url":"https://doi.org/10.1128/aem.65.5.1915-1918.1999","pdf_url":"https://aem.asm.org/content/aem/65/5/1915.full.pdf","source":{"id":"https://openalex.org/S158228368","display_name":"Applied and Environmental Microbiology","issn_l":"0099-2240","issn":["0099-2240","1070-6291","1098-5336"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied and Environmental Microbiology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://aem.asm.org/content/aem/65/5/1915.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5041102310","display_name":"Yanming Han","orcid":"https://orcid.org/0000-0002-0773-1936","observed_orcids":["https://orcid.org/0000-0002-0773-1936"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Yanming Han","raw_affiliation_strings":["<!--label omitted: 1-->Department of Animal Science1 and","Department of Animal Science1 and"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"<!--label omitted: 1-->Department of Animal Science1 and","institution_ids":[]},{"raw_affiliation_string":"Department of Animal Science1 and","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5017899142","display_name":"David Brown Wilson","orcid":"https://orcid.org/0000-0002-1826-7745","observed_orcids":["https://orcid.org/0000-0002-1826-7745"]},"institutions":[{"id":"https://openalex.org/I205783295","display_name":"Cornell University","ror":"https://ror.org/05bnh6r87","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"David B. Wilson","raw_affiliation_strings":["<!--label omitted: 2-->Section of Biochemistry, Molecular and Cell Biology,2 Cornell University, Ithaca, New York 14853","Section of Biochemistry, Molecular and Cell Biology,2 Cornell University, Ithaca, New York 14853"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"<!--label omitted: 2-->Section of Biochemistry, Molecular and Cell Biology,2 Cornell University, Ithaca, New York 14853","institution_ids":["https://openalex.org/I205783295"]},{"raw_affiliation_string":"Section of Biochemistry, Molecular and Cell Biology,2 Cornell University, Ithaca, New York 14853","institution_ids":["https://openalex.org/I205783295"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5091823146","display_name":"Xin Gen Lei","orcid":"https://orcid.org/0000-0002-2726-0684","observed_orcids":["https://orcid.org/0000-0002-2726-0684"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Xin gen Lei","raw_affiliation_strings":["<!--label omitted: 1-->Department of Animal Science1 and","Department of Animal Science1 and"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"<!--label omitted: 1-->Department of Animal Science1 and","institution_ids":[]},{"raw_affiliation_string":"Department of Animal Science1 and","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":8.7926,"has_fulltext":false,"cited_by_count":136,"citation_normalized_percentile":{"value":0.97698773,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":87,"max":99},"biblio":{"volume":"65","issue":"5","first_page":"1915","last_page":"1918"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12991","display_name":"Phytase and its Applications","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12991","display_name":"Phytase and its Applications","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/phytase","display_name":"phytase","score":0.9670000076293945},{"id":"https://openalex.org/keywords/aspergillus-niger","display_name":"Aspergillus niger","score":0.8769999742507935},{"id":"https://openalex.org/keywords/phya-gene","display_name":"phyA gene","score":0.9120000004768372},{"id":"https://openalex.org/keywords/saccharomyces-cerevisiae","display_name":"Saccharomyces cerevisiae","score":0.8899999856948853},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.8029999732971191},{"id":"https://openalex.org/keywords/glycosylation","display_name":"glycosylation","score":0.8870000243186951},{"id":"https://openalex.org/keywords/thermal-stability","display_name":"thermal stability","score":0.9300000071525574},{"id":"https://openalex.org/keywords/extracellular-enzymes","display_name":"extracellular enzymes","score":0.6600000262260437},{"id":"https://openalex.org/keywords/signal-peptide","display_name":"signal peptide","score":0.7940000295639038},{"id":"https://openalex.org/keywords/corn-meal","display_name":"corn meal","score":0.6629999876022339},{"id":"https://openalex.org/keywords/soybean-meal","display_name":"soybean meal","score":0.9800000190734863},{"id":"https://openalex.org/keywords/phosphorus-pollution","display_name":"phosphorus pollution","score":0.5699999928474426}],"concepts":[{"id":"https://openalex.org/C109551439","wikidata":"https://www.wikidata.org/wiki/Q410099","display_name":"Phytase","level":3,"score":0.9897435307502747},{"id":"https://openalex.org/C2781216036","wikidata":"https://www.wikidata.org/wiki/Q902494","display_name":"Thermostability","level":3,"score":0.830061674118042},{"id":"https://openalex.org/C2775960407","wikidata":"https://www.wikidata.org/wiki/Q132014","display_name":"Aspergillus niger","level":2,"score":0.7912231683731079},{"id":"https://openalex.org/C2777576037","wikidata":"https://www.wikidata.org/wiki/Q719725","display_name":"Saccharomyces cerevisiae","level":3,"score":0.6971046924591064},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6181507706642151},{"id":"https://openalex.org/C2777313579","wikidata":"https://www.wikidata.org/wiki/Q898365","display_name":"Glycosylation","level":2,"score":0.5256922245025635},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.49498075246810913},{"id":"https://openalex.org/C10858879","wikidata":"https://www.wikidata.org/wiki/Q899790","display_name":"Signal peptide","level":4,"score":0.44055694341659546},{"id":"https://openalex.org/C28406088","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Extracellular","level":2,"score":0.4201773703098297},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.3907870948314667},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.3699268102645874},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3529619872570038},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.31639528274536133}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000908","descriptor_name":"Antibodies, Fungal","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001234","descriptor_name":"Aspergillus niger","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D001234","descriptor_name":"Aspergillus niger","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004795","descriptor_name":"Enzyme Stability","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005800","descriptor_name":"Genes, Fungal","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D006031","descriptor_name":"Glycosylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010758","descriptor_name":"Phosphorus","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010832","descriptor_name":"6-Phytase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D010832","descriptor_name":"6-Phytase","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D010832","descriptor_name":"6-Phytase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010833","descriptor_name":"Phytic Acid","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011817","descriptor_name":"Rabbits","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012441","descriptor_name":"Saccharomyces cerevisiae","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D013696","descriptor_name":"Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015870","descriptor_name":"Gene Expression","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017931","descriptor_name":"DNA Primers","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1128/aem.65.5.1915-1918.1999","doi":"https://doi.org/10.1128/aem.65.5.1915-1918.1999","is_oa":true,"landing_page_url":"https://doi.org/10.1128/aem.65.5.1915-1918.1999","pdf_url":"https://aem.asm.org/content/aem/65/5/1915.full.pdf","source":{"id":"https://openalex.org/S158228368","display_name":"Applied and Environmental Microbiology","issn_l":"0099-2240","issn":["0099-2240","1070-6291","1098-5336"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied and Environmental Microbiology","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:154151","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC91276","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},{"id":"pmid:10223979","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10223979","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied and environmental microbiology","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:91276","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC91276/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Appl Environ Microbiol","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1128/aem.65.5.1915-1918.1999","doi":"https://doi.org/10.1128/aem.65.5.1915-1918.1999","is_oa":true,"landing_page_url":"https://doi.org/10.1128/aem.65.5.1915-1918.1999","pdf_url":"https://aem.asm.org/content/aem/65/5/1915.full.pdf","source":{"id":"https://openalex.org/S158228368","display_name":"Applied and Environmental Microbiology","issn_l":"0099-2240","issn":["0099-2240","1070-6291","1098-5336"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied and Environmental Microbiology","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.5666999816894531,"id":"https://metadata.un.org/sdg/2","display_name":"Zero hunger"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":false},"content_urls":{"pdf":"https://content.openalex.org/works/W2161663291.pdf"},"referenced_works_count":31,"referenced_works":["https://openalex.org/W66405674","https://openalex.org/W189241624","https://openalex.org/W341787665","https://openalex.org/W1492171676","https://openalex.org/W1514220652","https://openalex.org/W1522893583","https://openalex.org/W1533167872","https://openalex.org/W1555601337","https://openalex.org/W1587967271","https://openalex.org/W1599268510","https://openalex.org/W1775749144","https://openalex.org/W1973782344","https://openalex.org/W1985342306","https://openalex.org/W1985644869","https://openalex.org/W1987140209","https://openalex.org/W2008765994","https://openalex.org/W2018289835","https://openalex.org/W2024429016","https://openalex.org/W2033447505","https://openalex.org/W2034911191","https://openalex.org/W2049130859","https://openalex.org/W2058940030","https://openalex.org/W2074591873","https://openalex.org/W2088872234","https://openalex.org/W2089504004","https://openalex.org/W2090623403","https://openalex.org/W2100837269","https://openalex.org/W2111281542","https://openalex.org/W2146633888","https://openalex.org/W2147418323","https://openalex.org/W4232496949"],"related_works":["https://openalex.org/W2083040603","https://openalex.org/W2400401571","https://openalex.org/W2359421145","https://openalex.org/W2017927595","https://openalex.org/W2357677587","https://openalex.org/W2019684655","https://openalex.org/W2007688779","https://openalex.org/W2149776305","https://openalex.org/W2033447505","https://openalex.org/W2357649661"],"abstract_inverted_index":{"Phytase":[0],"improves":[1],"the":[2,38,43,53,57,63,88,92,128,133,136,154,159,192],"bioavailability":[3],"of":[4,18,40,56,80,143,158,164,166,171],"phytate":[5,181],"phosphorus":[6,16,182],"in":[7,32,70,162,179,188,202],"plant":[8],"foods":[9],"to":[10,24,36,103,107,132,149],"humans":[11],"and":[12,14,35,46,67,91,105,109,115,119,147,169,205],"animals":[13],"reduces":[15],"pollution":[17],"animal":[19],"waste.":[20],"Our":[21],"objectives":[22],"were":[23],"express":[25],"an":[26,74,198],"Aspergillus":[27],"niger":[28],"phytase":[29,83,97,138,160,176,201],"gene":[30,59,194],"(phyA)":[31],"Saccharomyces":[33],"cerevisiae":[34,72],"determine":[37],"effects":[39],"glycosylation":[41],"on":[42],"phytase's":[44],"activity":[45,84,168],"thermostability.":[47,173],"A":[48],"1.4-kb":[49],"DNA":[50],"fragment":[51],"containing":[52],"coding":[54],"region":[55],"phyA":[58,193],"was":[60,68,85,177,195,208],"inserted":[61],"into":[62],"expression":[64],"vector":[65],"pYES2":[66],"expressed":[69,96,137,196],"S.":[71,203],"as":[73,197],"active,":[75,199],"extracellular":[76,82,200],"phytase.":[77],"The":[78,95,174],"yield":[79],"total":[81],"affected":[86,209],"by":[87,210],"signal":[89],"peptide":[90],"medium":[93],"composition.":[94],"had":[98,139],"two":[99],"pH":[100],"optima":[101],"(2":[102],"2.5":[104],"5":[106],"5.5)":[108],"a":[110,123,140],"temperature":[111],"optimum":[112],"between":[113],"55":[114],"60":[116],"degrees":[117],"C,":[118],"it":[120],"cross-reacted":[121],"with":[122],"rabbit":[124],"polyclonal":[125],"antibody":[126],"against":[127],"wild-type":[129],"enzyme.":[130,156],"Due":[131],"heavy":[134],"glycosylation,":[135],"molecular":[141],"size":[142],"approximately":[144],"120":[145],"kDa":[146],"appeared":[148],"be":[150],"more":[151],"thermostable":[152],"than":[153],"commercial":[155],"Deglycosylation":[157],"resulted":[161],"losses":[163],"9%":[165],"its":[167,172,206],"40%":[170],"recombinant":[175],"effective":[178],"hydrolyzing":[180],"from":[183],"corn":[184],"or":[185],"soybean":[186],"meal":[187],"vitro.":[189],"In":[190],"conclusion,":[191],"cerevisiae,":[204],"thermostability":[207],"glycosylation.":[211]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":2},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":4},{"year":2018,"cited_by_count":4},{"year":2017,"cited_by_count":6},{"year":2016,"cited_by_count":3},{"year":2015,"cited_by_count":5},{"year":2014,"cited_by_count":7},{"year":2013,"cited_by_count":4},{"year":2012,"cited_by_count":5}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2016-06-24T00:00:00"}
