{"id":"https://openalex.org/W2161355985","doi":"https://doi.org/10.1111/j.1432-1033.1973.tb02905.x","title":"The Mechanism of Action of Methionyl\u2010tRNA Synthetase","display_name":"The Mechanism of Action of Methionyl\u2010tRNA Synthetase","publication_year":1973,"publication_date":"1973-07-01","ids":{"openalex":"https://openalex.org/W2161355985","doi":"https://doi.org/10.1111/j.1432-1033.1973.tb02905.x","mag":"2161355985","pmid":"https://pubmed.ncbi.nlm.nih.gov/4581819"},"language":"en","primary_location":{"id":"doi:10.1111/j.1432-1033.1973.tb02905.x","doi":"https://doi.org/10.1111/j.1432-1033.1973.tb02905.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1432-1033.1973.tb02905.x","pdf_url":null,"source":{"id":"https://openalex.org/S4210214924","display_name":"European Journal of Biochemistry","issn_l":"0014-2956","issn":["0014-2956","0945-5795","1432-1033"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","poland-100"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"European Journal of Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5108457619","display_name":"Fran\u00e7oise Lawrence","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I4210099362","display_name":"Laboratoire d'Enzymologie et Biochimie Structurales","ror":"https://ror.org/00j1y9t40","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I277688954","https://openalex.org/I4210096427","https://openalex.org/I4210099362"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Fran\u00e7oise Lawrence","raw_affiliation_strings":["Laboratoire d'Enzymologie du C.N.R.S., B-91190 Gif-sur-Yvette, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratoire d'Enzymologie du C.N.R.S., B-91190 Gif-sur-Yvette, France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I4210099362"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5108203800","display_name":"Sylvain Blanquet","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I4210099362","display_name":"Laboratoire d'Enzymologie et Biochimie Structurales","ror":"https://ror.org/00j1y9t40","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I277688954","https://openalex.org/I4210096427","https://openalex.org/I4210099362"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Sylvain Blanquet","raw_affiliation_strings":["Laboratoire d'Enzymologie du C.N.R.S., B-91190 Gif-sur-Yvette, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratoire d'Enzymologie du C.N.R.S., B-91190 Gif-sur-Yvette, France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I4210099362"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5000987484","display_name":"Maryse Poiret","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I4210099362","display_name":"Laboratoire d'Enzymologie et Biochimie Structurales","ror":"https://ror.org/00j1y9t40","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I277688954","https://openalex.org/I4210096427","https://openalex.org/I4210099362"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Maryse Poiret","raw_affiliation_strings":["Laboratoire d'Enzymologie du C.N.R.S., B-91190 Gif-sur-Yvette, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratoire d'Enzymologie du C.N.R.S., B-91190 Gif-sur-Yvette, France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I4210099362"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5063888461","display_name":"Malka Robert-G\u00e9ro","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I4210099362","display_name":"Laboratoire d'Enzymologie et Biochimie Structurales","ror":"https://ror.org/00j1y9t40","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I277688954","https://openalex.org/I4210096427","https://openalex.org/I4210099362"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Malka Robert\u2010Gero","raw_affiliation_strings":["Laboratoire d'Enzymologie du C.N.R.S., B-91190 Gif-sur-Yvette, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratoire d'Enzymologie du C.N.R.S., B-91190 Gif-sur-Yvette, France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I4210099362"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5110582598","display_name":"Jean\u2010Pierre Waller","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I4210099362","display_name":"Laboratoire d'Enzymologie et Biochimie Structurales","ror":"https://ror.org/00j1y9t40","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I277688954","https://openalex.org/I4210096427","https://openalex.org/I4210099362"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Jean\u2010Pierre Waller","raw_affiliation_strings":["Laboratoire d'Enzymologie du C.N.R.S., B-91190 Gif-sur-Yvette, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratoire d'Enzymologie du C.N.R.S., B-91190 Gif-sur-Yvette, France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I4210099362"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":3.5129,"has_fulltext":false,"cited_by_count":92,"citation_normalized_percentile":{"value":0.93958793,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"36","issue":"1","first_page":"234","last_page":"243"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11482","display_name":"RNA modifications and cancer","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12770","display_name":"Amino Acid Enzymes and Metabolism","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/methionyl-trna-synthetase","display_name":"methionyl-tRNA synthetase","score":0.9990000128746033},{"id":"https://openalex.org/keywords/enzyme-mechanism","display_name":"enzyme mechanism","score":0.7229999899864197},{"id":"https://openalex.org/keywords/atp-ppi-exchange","display_name":"ATP-PPi exchange","score":0.9369999766349792},{"id":"https://openalex.org/keywords/aminoacyl-trna-formation","display_name":"aminoacyl-tRNA formation","score":0.878000020980835},{"id":"https://openalex.org/keywords/methionine","display_name":"methionine","score":0.5400000214576721},{"id":"https://openalex.org/keywords/atp-inhibition","display_name":"ATP inhibition","score":0.8019999861717224},{"id":"https://openalex.org/keywords/ionic-requirements","display_name":"ionic requirements","score":0.8090000152587891},{"id":"https://openalex.org/keywords/magnesium","display_name":"magnesium","score":0.5960000157356262},{"id":"https://openalex.org/keywords/spermine","display_name":"spermine","score":0.8999999761581421},{"id":"https://openalex.org/keywords/spermidine","display_name":"spermidine","score":0.9959999918937683},{"id":"https://openalex.org/keywords/ammonium-chloride","display_name":"ammonium chloride","score":0.7580000162124634},{"id":"https://openalex.org/keywords/trnafmet","display_name":"tRNAfMet","score":0.7210000157356262},{"id":"https://openalex.org/keywords/aminoacylation","display_name":"aminoacylation","score":0.5709999799728394}],"concepts":[{"id":"https://openalex.org/C151238385","wikidata":"https://www.wikidata.org/wiki/Q4746444","display_name":"Aminoacylation","level":5,"score":0.8372853994369507},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7037777304649353},{"id":"https://openalex.org/C153957851","wikidata":"https://www.wikidata.org/wiki/Q201448","display_name":"Transfer RNA","level":4,"score":0.6493180394172668},{"id":"https://openalex.org/C2780340462","wikidata":"https://www.wikidata.org/wiki/Q127450","display_name":"Trypsin","level":3,"score":0.6272426247596741},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.6252890825271606},{"id":"https://openalex.org/C2777497464","wikidata":"https://www.wikidata.org/wiki/Q418834","display_name":"Spermidine","level":3,"score":0.5901214480400085},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.43200162053108215},{"id":"https://openalex.org/C2780912031","wikidata":"https://www.wikidata.org/wiki/Q180341","display_name":"Methionine","level":3,"score":0.4292728304862976},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.4098547697067261},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.14045101404190063},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.09888210892677307},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000215","descriptor_name":"Acylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000255","descriptor_name":"Adenosine Triphosphate","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000604","descriptor_name":"Amino Acyl-tRNA Synthetases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D000643","descriptor_name":"Ammonium Chloride","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002384","descriptor_name":"Catalysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008274","descriptor_name":"Magnesium","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008715","descriptor_name":"Methionine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011189","descriptor_name":"Potassium Chloride","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011487","descriptor_name":"Protein Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012343","descriptor_name":"RNA, Transfer","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013050","descriptor_name":"Spectrometry, Fluorescence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013095","descriptor_name":"Spermidine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013096","descriptor_name":"Spermine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013329","descriptor_name":"Structure-Activity Relationship","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014357","descriptor_name":"Trypsin","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1111/j.1432-1033.1973.tb02905.x","doi":"https://doi.org/10.1111/j.1432-1033.1973.tb02905.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1432-1033.1973.tb02905.x","pdf_url":null,"source":{"id":"https://openalex.org/S4210214924","display_name":"European Journal of Biochemistry","issn_l":"0014-2956","issn":["0014-2956","0945-5795","1432-1033"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","poland-100"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"European Journal of Biochemistry","raw_type":"journal-article"},{"id":"pmid:4581819","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/4581819","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"European journal of biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":18,"referenced_works":["https://openalex.org/W1027697937","https://openalex.org/W1489905936","https://openalex.org/W1503398493","https://openalex.org/W1970474129","https://openalex.org/W1978084329","https://openalex.org/W1981096799","https://openalex.org/W2001686780","https://openalex.org/W2005700051","https://openalex.org/W2011878394","https://openalex.org/W2013199493","https://openalex.org/W2026912546","https://openalex.org/W2045401792","https://openalex.org/W2071243752","https://openalex.org/W2085815799","https://openalex.org/W2089840651","https://openalex.org/W2090069009","https://openalex.org/W2167926189","https://openalex.org/W3133270978"],"related_works":["https://openalex.org/W2615816587","https://openalex.org/W1970082042","https://openalex.org/W2038480690","https://openalex.org/W2015796254","https://openalex.org/W1994595236","https://openalex.org/W2012354655","https://openalex.org/W1565321325","https://openalex.org/W1965282172","https://openalex.org/W1987625224","https://openalex.org/W2075323803"],"abstract_inverted_index":{"The":[0,133],"catalytic":[1],"properties":[2],"of":[3,37,62,68,78,112,129,157,162,176,185],"native":[4,70,83,101,131],"and":[5,16,48,53,80,84,146],"trypsin\u2010modified":[6,85,107],"methionyl\u2010tRNA":[7,158],"synthetases":[8],"were":[9,140],"compared":[10],"in":[11,17,59,66,88,127,142,159,169,182],"the":[12,18,30,34,38,69,73,82,100,106,110,113,130,137,155,160,179,183],"ATP\u2010PPi":[13,31],"exchange":[14,32],"reaction":[15,19,74,139,180],"leading":[20,75],"to":[21,45,64,76,122,125,153],"aminoacyl\u2010tRNA":[22],"formation":[23,156],"performed":[24],"under":[25,117],"comparable":[26],"experimental":[27],"conditions.":[28],"In":[29,72,91],"reaction,":[33],"two":[35],"forms":[36],"enzyme":[39,102],"are":[40,58,120,151],"virtually":[41],"indistinguishable":[42],"with":[43,99,105],"respect":[44],"ionic":[46,134],"requirements":[47,135],"Michaelis":[49],"constants":[50],"for":[51,136],"ATP":[52,97],"methionine,":[54],"while":[55],"V":[56,114],"values":[57,115],"a":[60,93],"ratio":[61,111],"2":[63],"1":[65],"favor":[67,128],"enzyme.":[71,132],"aminoacylation":[77,138],"tRNAfMet":[79],"tRNAmMet,":[81],"enzymes":[86],"differ":[87],"several":[89],"respects.":[90],"particular,":[92],"pronounced":[94,173],"inhibition":[95],"by":[96],"occurs":[98],"but":[103,148],"not":[104,149],"form.":[108],"Also,":[109],"measured":[116],"optimal":[118],"conditions":[119],"closer":[121],"4":[123],"than":[124,168],"2,":[126],"examined":[141],"detail.":[143],"Spermine,":[144],"spermidine":[145,186],"NH4Cl,":[147],"KCl,":[150],"able":[152],"sustain":[154],"absence":[161],"magnesium,":[163],"albeit":[164],"at":[165],"lower":[166],"rates":[167],"its":[170],"presence.":[171],"A":[172],"synergistic":[174],"effect":[175],"MgCl2":[177],"on":[178],"catalyzed":[181],"presence":[184],"or":[187],"NH4Cl":[188],"is":[189],"described.":[190]},"counts_by_year":[{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":3},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}