{"id":"https://openalex.org/W2160925331","doi":"https://doi.org/10.1002/1522-2683(200102)22:4<729::aid-elps729>3.0.co;2-o","title":"DNA sequencing by capillary electrophoresis using copolymers of acrylamide andN,N-dimethyl-acrylamide","display_name":"DNA sequencing by capillary electrophoresis using copolymers of acrylamide andN,N-dimethyl-acrylamide","publication_year":2001,"publication_date":"2001-02-01","ids":{"openalex":"https://openalex.org/W2160925331","doi":"https://doi.org/10.1002/1522-2683(200102)22:4<729::aid-elps729>3.0.co;2-o","mag":"2160925331","pmid":"https://pubmed.ncbi.nlm.nih.gov/11296928"},"language":"en","primary_location":{"id":"doi:10.1002/1522-2683(200102)22:4<729::aid-elps729>3.0.co;2-o","doi":"https://doi.org/10.1002/1522-2683(200102)22:4<729::aid-elps729>3.0.co;2-o","is_oa":false,"landing_page_url":"https://doi.org/10.1002/1522-2683(200102)22:4<729::aid-elps729>3.0.co;2-o","pdf_url":null,"source":{"id":"https://openalex.org/S2849398","display_name":"Electrophoresis","issn_l":"0173-0835","issn":["0173-0835","1522-2683"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ELECTROPHORESIS","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5053218126","display_name":"Liguo Song","orcid":"https://orcid.org/0000-0002-8147-3381","observed_orcids":["https://orcid.org/0000-0002-8147-3381"]},"institutions":[{"id":"https://openalex.org/I1327163397","display_name":"State University of New York","ror":"https://ror.org/01q1z8k08","country_code":"US","type":"education","lineage":["https://openalex.org/I1327163397"]},{"id":"https://openalex.org/I59553526","display_name":"Stony Brook University","ror":"https://ror.org/05qghxh33","country_code":"US","type":"education","lineage":["https://openalex.org/I59553526"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Liguo Song","raw_affiliation_strings":["Chemistry Department, State University of New York at Stony Brook, 11794-3400, USA","Chemistry Department, State University of New York at Stony Brook, NY, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Chemistry Department, State University of New York at Stony Brook, 11794-3400, USA","institution_ids":["https://openalex.org/I1327163397","https://openalex.org/I59553526"]},{"raw_affiliation_string":"Chemistry Department, State University of New York at Stony Brook, NY, USA","institution_ids":["https://openalex.org/I1327163397","https://openalex.org/I59553526"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5005864617","display_name":"Dehai Liang","orcid":"https://orcid.org/0000-0003-4246-050X","observed_orcids":["https://orcid.org/0000-0003-4246-050X"]},"institutions":[{"id":"https://openalex.org/I1327163397","display_name":"State University of New York","ror":"https://ror.org/01q1z8k08","country_code":"US","type":"education","lineage":["https://openalex.org/I1327163397"]},{"id":"https://openalex.org/I59553526","display_name":"Stony Brook University","ror":"https://ror.org/05qghxh33","country_code":"US","type":"education","lineage":["https://openalex.org/I59553526"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Dehai Liang","raw_affiliation_strings":["Chemistry Department, State University of New York at Stony Brook, NY, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Chemistry Department, State University of New York at Stony Brook, NY, USA","institution_ids":["https://openalex.org/I1327163397","https://openalex.org/I59553526"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5023740030","display_name":"Jan Kielescawa","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I200870766","display_name":"Brookhaven National Laboratory","ror":"https://ror.org/02ex6cf31","country_code":"US","type":"facility","lineage":["https://openalex.org/I1330989302","https://openalex.org/I200870766","https://openalex.org/I39565521","https://openalex.org/I4210142672"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jan Kielescawa","raw_affiliation_strings":["Biology Department, Brookhaven National Laboratory, Upton, NY, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biology Department, Brookhaven National Laboratory, Upton, NY, USA","institution_ids":["https://openalex.org/I200870766"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5102490919","display_name":"Jason Liang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2801372796","display_name":"Glasgow Kelvin College","ror":"https://ror.org/04v62ha11","country_code":"GB","type":"education","lineage":["https://openalex.org/I2801372796"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Jason Liang","raw_affiliation_strings":["The Wheatley School, Old Westbury, NY, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Wheatley School, Old Westbury, NY, USA","institution_ids":["https://openalex.org/I2801372796"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5072515793","display_name":"Edward Tjoe","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1327163397","display_name":"State University of New York","ror":"https://ror.org/01q1z8k08","country_code":"US","type":"education","lineage":["https://openalex.org/I1327163397"]},{"id":"https://openalex.org/I59553526","display_name":"Stony Brook University","ror":"https://ror.org/05qghxh33","country_code":"US","type":"education","lineage":["https://openalex.org/I59553526"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Edward Tjoe","raw_affiliation_strings":["Chemistry Department, State University of New York at Stony Brook, NY, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Chemistry Department, State University of New York at Stony Brook, NY, USA","institution_ids":["https://openalex.org/I1327163397","https://openalex.org/I59553526"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103912021","display_name":"Dufei Fang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1327163397","display_name":"State University of New York","ror":"https://ror.org/01q1z8k08","country_code":"US","type":"education","lineage":["https://openalex.org/I1327163397"]},{"id":"https://openalex.org/I59553526","display_name":"Stony Brook University","ror":"https://ror.org/05qghxh33","country_code":"US","type":"education","lineage":["https://openalex.org/I59553526"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Dufei Fang","raw_affiliation_strings":["Chemistry Department, State University of New York at Stony Brook, NY, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Chemistry Department, State University of New York at Stony Brook, NY, USA","institution_ids":["https://openalex.org/I1327163397","https://openalex.org/I59553526"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5000613784","display_name":"Benjamin Chu","orcid":"https://orcid.org/0000-0003-2775-2336","observed_orcids":["https://orcid.org/0000-0003-2775-2336"]},"institutions":[{"id":"https://openalex.org/I1327163397","display_name":"State University of New York","ror":"https://ror.org/01q1z8k08","country_code":"US","type":"education","lineage":["https://openalex.org/I1327163397"]},{"id":"https://openalex.org/I59553526","display_name":"Stony Brook University","ror":"https://ror.org/05qghxh33","country_code":"US","type":"education","lineage":["https://openalex.org/I59553526"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Benjamin Chu","raw_affiliation_strings":["Chemistry Department, State University of New York at Stony Brook, NY, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Chemistry Department, State University of New York at Stony Brook, NY, USA","institution_ids":["https://openalex.org/I1327163397","https://openalex.org/I59553526"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5000613784"],"corresponding_institution_ids":["https://openalex.org/I1327163397","https://openalex.org/I59553526"],"apc_list":{"value":3940,"currency":"USD","value_usd":3940},"apc_paid":null,"fwci":4.7695,"has_fulltext":false,"cited_by_count":29,"citation_normalized_percentile":{"value":0.95220496,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":"22","issue":"4","first_page":"729","last_page":"736"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10412","display_name":"Microfluidic and Capillary Electrophoresis Applications","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10412","display_name":"Microfluidic and Capillary Electrophoresis Applications","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10908","display_name":"Analytical Chemistry and Chromatography","score":0.00039999998989515007,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dna-sequencing","display_name":"DNA sequencing","score":0.9229999780654907},{"id":"https://openalex.org/keywords/capillary-electrophoresis","display_name":"capillary electrophoresis","score":0.9940000176429749},{"id":"https://openalex.org/keywords/acrylamide-copolymers","display_name":"acrylamide copolymers","score":0.9169999957084656},{"id":"https://openalex.org/keywords/n-n-dimethylacrylamide","display_name":"N,N-dimethylacrylamide","score":0.9980000257492065},{"id":"https://openalex.org/keywords/polyacrylamide","display_name":"polyacrylamide","score":0.9300000071525574},{"id":"https://openalex.org/keywords/separation-media","display_name":"separation media","score":0.7960000038146973},{"id":"https://openalex.org/keywords/polymer-coatings","display_name":"polymer coatings","score":0.3799999952316284},{"id":"https://openalex.org/keywords/hydrophilicity","display_name":"hydrophilicity","score":0.8080000281333923},{"id":"https://openalex.org/keywords/separation-efficiency","display_name":"separation efficiency","score":0.8059999942779541},{"id":"https://openalex.org/keywords/base-resolution","display_name":"base resolution","score":0.5820000171661377},{"id":"https://openalex.org/keywords/molar-ratio","display_name":"molar ratio","score":0.5559999942779541}],"concepts":[{"id":"https://openalex.org/C15920480","wikidata":"https://www.wikidata.org/wiki/Q421281","display_name":"Copolymer","level":3,"score":0.8954399228096008},{"id":"https://openalex.org/C2779402116","wikidata":"https://www.wikidata.org/wiki/Q342939","display_name":"Acrylamide","level":4,"score":0.879116415977478},{"id":"https://openalex.org/C2900643","wikidata":"https://www.wikidata.org/wiki/Q747468","display_name":"Capillary electrophoresis","level":2,"score":0.8116381168365479},{"id":"https://openalex.org/C2778862431","wikidata":"https://www.wikidata.org/wiki/Q416002","display_name":"Polyacrylamide","level":2,"score":0.6801860928535461},{"id":"https://openalex.org/C521977710","wikidata":"https://www.wikidata.org/wiki/Q81163","display_name":"Polymer","level":2,"score":0.6497097015380859},{"id":"https://openalex.org/C40250595","wikidata":"https://www.wikidata.org/wiki/Q185098","display_name":"Electrophoresis","level":2,"score":0.4894157946109772},{"id":"https://openalex.org/C2994067411","wikidata":"https://www.wikidata.org/wiki/Q125264","display_name":"Molar ratio","level":3,"score":0.43469518423080444},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.43130019307136536},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.4063495397567749},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4047953188419342},{"id":"https://openalex.org/C188027245","wikidata":"https://www.wikidata.org/wiki/Q750446","display_name":"Polymer chemistry","level":1,"score":0.35406196117401123},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.10825547575950623},{"id":"https://openalex.org/C161790260","wikidata":"https://www.wikidata.org/wiki/Q82264","display_name":"Catalysis","level":2,"score":0.04362398386001587}],"mesh":[{"descriptor_ui":"D000178","descriptor_name":"Acrylamides","qualifier_ui":"Q000138","qualifier_name":"chemical synthesis","is_major_topic":false},{"descriptor_ui":"D000178","descriptor_name":"Acrylamides","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D008970","descriptor_name":"Molecular Weight","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017422","descriptor_name":"Sequence Analysis, DNA","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D019075","descriptor_name":"Electrophoresis, Capillary","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1002/1522-2683(200102)22:4<729::aid-elps729>3.0.co;2-o","doi":"https://doi.org/10.1002/1522-2683(200102)22:4<729::aid-elps729>3.0.co;2-o","is_oa":false,"landing_page_url":"https://doi.org/10.1002/1522-2683(200102)22:4<729::aid-elps729>3.0.co;2-o","pdf_url":null,"source":{"id":"https://openalex.org/S2849398","display_name":"Electrophoresis","issn_l":"0173-0835","issn":["0173-0835","1522-2683"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ELECTROPHORESIS","raw_type":"journal-article"},{"id":"pmid:11296928","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/11296928","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Electrophoresis","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2093711786","display_name":null,"funder_award_id":"1R01 AI22222","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"},{"id":"https://openalex.org/G3360790532","display_name":null,"funder_award_id":"2R01HG 0138604","funder_id":"https://openalex.org/F4320337348","funder_display_name":"National Human Genome Research Institute"}],"funders":[{"id":"https://openalex.org/F4320337348","display_name":"National Human Genome Research Institute","ror":"https://ror.org/00baak391"},{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":51,"referenced_works":["https://openalex.org/W1566865222","https://openalex.org/W1926033671","https://openalex.org/W1967867237","https://openalex.org/W1972540880","https://openalex.org/W1976840126","https://openalex.org/W1977924644","https://openalex.org/W1979514083","https://openalex.org/W1980960468","https://openalex.org/W1985830439","https://openalex.org/W1986288394","https://openalex.org/W1993333793","https://openalex.org/W1995310354","https://openalex.org/W2000150613","https://openalex.org/W2000481104","https://openalex.org/W2001479907","https://openalex.org/W2008965264","https://openalex.org/W2014454097","https://openalex.org/W2016965829","https://openalex.org/W2019249659","https://openalex.org/W2019732510","https://openalex.org/W2022378704","https://openalex.org/W2024588177","https://openalex.org/W2034475812","https://openalex.org/W2034710803","https://openalex.org/W2035058958","https://openalex.org/W2036081249","https://openalex.org/W2036582173","https://openalex.org/W2037584869","https://openalex.org/W2045276203","https://openalex.org/W2047013441","https://openalex.org/W2048655993","https://openalex.org/W2058349746","https://openalex.org/W2058877820","https://openalex.org/W2060884975","https://openalex.org/W2063506093","https://openalex.org/W2065303458","https://openalex.org/W2075629170","https://openalex.org/W2076931998","https://openalex.org/W2082765979","https://openalex.org/W2083726881","https://openalex.org/W2086836596","https://openalex.org/W2090388952","https://openalex.org/W2093071344","https://openalex.org/W2094525645","https://openalex.org/W2121829061","https://openalex.org/W2125157261","https://openalex.org/W2135025103","https://openalex.org/W2150246155","https://openalex.org/W2151275400","https://openalex.org/W2161471331","https://openalex.org/W2163934801"],"related_works":["https://openalex.org/W2000941898","https://openalex.org/W2019020935","https://openalex.org/W1968882853","https://openalex.org/W2019333164","https://openalex.org/W1989190771","https://openalex.org/W2076686296","https://openalex.org/W2078387212","https://openalex.org/W2021098288","https://openalex.org/W2034710803","https://openalex.org/W4238626127"],"abstract_inverted_index":{"Copolymers":[0],"of":[1,13,21,46,52,66,101,146],"acrylamide":[2],"(AM)":[3],"and":[4,16,18,49,115,152],"N,N-dimethylacrylamide":[5],"(DMA)":[6],"with":[7,105,119,123,139],"AM":[8,107],"to":[9,108,149,155],"DMA":[10,109],"molar":[11,110],"ratios":[12],"3:1,":[14,113],"2:1":[15,114],"1:1":[17],"molecular":[19],"weights":[20],"about":[22,162],"2.2":[23],"MDa":[24],"were":[25,29],"synthesized.":[26],"The":[27,42],"polymers":[28],"tested":[30],"as":[31],"separation":[32,64,90,99,131],"media":[33],"in":[34,59,161],"DNA":[35,96],"sequencing":[36],"analysis":[37],"by":[38,77],"capillary":[39],"electrophoresis":[40],"(CE).":[41],"dynamic":[43],"coating":[44,86],"ability":[45,100],"polydimethylacrylamide":[47],"(PDMA)":[48],"the":[50,79,83,98,102,121],"hydrophilicity":[51],"polyacrylamide":[53],"(PAM)":[54],"have":[55,158],"been":[56,75,159],"successfully":[57],"combined":[58],"these":[60],"random":[61],"copolymers.":[62],"A":[63],"efficiency":[65],"over":[67],"10":[68],"million":[69],"theoretical":[70],"plates":[71],"per":[72],"meter":[73],"has":[74],"reached":[76],"using":[78,134],"bare":[80],"capillaries":[81],"without":[82],"additional":[84],"polymer":[85],"step.":[87],"Under":[88],"optimized":[89],"conditions":[91],"for":[92],"longer":[93],"read":[94],"length":[95],"sequencing,":[97],"copolymers":[103],"decreased":[104],"decreasing":[106],"ratio":[111,127],"from":[112],"1:1.":[116],"In":[117],"comparison":[118],"PAM,":[120],"copolymer":[122,138],"a":[124,129,135],"3:1":[125,140],"AM:DMA":[126,141],"showed":[128],"higher":[130],"efficiency.":[132],"By":[133],"2.5%":[136],"w/v":[137],"ratio,":[142],"one":[143],"base":[144],"resolution":[145],"0.55":[147],"up":[148,154],"699":[150],"bases":[151,157],"0.30":[153],"963":[156],"achieved":[160],"80":[163],"min":[164],"at":[165],"ambient":[166],"temperatures.":[167]},"counts_by_year":[{"year":2022,"cited_by_count":1},{"year":2012,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}