{"id":"https://openalex.org/W2160378368","doi":"https://doi.org/10.1101/pdb.prot5041","title":"Preparation of Mouse Embryonic Fibroblast Feeder Cells for Human Embryonic Stem Cell Culture","display_name":"Preparation of Mouse Embryonic Fibroblast Feeder Cells for Human Embryonic Stem Cell Culture","publication_year":2008,"publication_date":"2008-09-01","ids":{"openalex":"https://openalex.org/W2160378368","doi":"https://doi.org/10.1101/pdb.prot5041","mag":"2160378368","pmid":"https://pubmed.ncbi.nlm.nih.gov/21356910"},"language":"en","primary_location":{"id":"doi:10.1101/pdb.prot5041","doi":"https://doi.org/10.1101/pdb.prot5041","is_oa":false,"landing_page_url":"https://doi.org/10.1101/pdb.prot5041","pdf_url":null,"source":{"id":"https://openalex.org/S119953358","display_name":"Cold Spring Harbor Protocols","issn_l":"1559-6095","issn":["1559-6095","1940-3402"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315909","host_organization_name":"Cold Spring Harbor Laboratory Press","host_organization_lineage":["https://openalex.org/P4310315909"],"host_organization_lineage_names":["Cold Spring Harbor Laboratory Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cold Spring Harbor Protocols","raw_type":"journal-article"},"volume":null,"type":"preprint","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5002671369","display_name":"Sohyun Lee McElroy","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4394709089","display_name":"Institute for Stem Cell Biology and Regenerative Medicine","ror":"https://ror.org/02vbab064","country_code":"US","type":"facility","lineage":["https://openalex.org/I4210137306","https://openalex.org/I4394709089","https://openalex.org/I97018004"]},{"id":"https://openalex.org/I97018004","display_name":"Stanford University","ror":"https://ror.org/00f54p054","country_code":"US","type":"education","lineage":["https://openalex.org/I97018004"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Sohyun L. McElroy","raw_affiliation_strings":["Center for Human Embryonic Stem Cell Research and Education, Institute for Stem Cell Biology and Regenerative Medicine, Department of Obstetrics and Gynecology, Stanford University, Palo Alto, CA 94304-5542, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Human Embryonic Stem Cell Research and Education, Institute for Stem Cell Biology and Regenerative Medicine, Department of Obstetrics and Gynecology, Stanford University, Palo Alto, CA 94304-5542, USA","institution_ids":["https://openalex.org/I4394709089","https://openalex.org/I97018004"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5075866851","display_name":"Renee A. Reijo Pera","orcid":"https://orcid.org/0000-0002-6487-1329","observed_orcids":["https://orcid.org/0000-0002-6487-1329"]},"institutions":[{"id":"https://openalex.org/I4394709089","display_name":"Institute for Stem Cell Biology and Regenerative Medicine","ror":"https://ror.org/02vbab064","country_code":"US","type":"facility","lineage":["https://openalex.org/I4210137306","https://openalex.org/I4394709089","https://openalex.org/I97018004"]},{"id":"https://openalex.org/I97018004","display_name":"Stanford University","ror":"https://ror.org/00f54p054","country_code":"US","type":"education","lineage":["https://openalex.org/I97018004"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Renee A. Reijo Pera","raw_affiliation_strings":["Center for Human Embryonic Stem Cell Research and Education, Institute for Stem Cell Biology and Regenerative Medicine, Department\n                           of Obstetrics and Gynecology, Stanford University, Palo Alto, CA 94304-5542, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Human Embryonic Stem Cell Research and Education, Institute for Stem Cell Biology and Regenerative Medicine, Department\n                           of Obstetrics and Gynecology, Stanford University, Palo Alto, CA 94304-5542, USA","institution_ids":["https://openalex.org/I4394709089","https://openalex.org/I97018004"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":15,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"2008","issue":"9","first_page":"pdb.prot5041","last_page":"pdb.prot5041"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10505","display_name":"Pluripotent Stem Cells Research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10505","display_name":"Pluripotent Stem Cells Research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10268","display_name":"Developmental Biology and Gene Regulation","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12929","display_name":"Animal Genetics and Reproduction","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/mouse-embryonic-fibroblasts","display_name":"mouse embryonic fibroblasts","score":0.9330000281333923},{"id":"https://openalex.org/keywords/feeder-cells","display_name":"feeder cells","score":0.8209999799728394},{"id":"https://openalex.org/keywords/human-embryonic-stem-cells","display_name":"human embryonic stem cells","score":0.9879999756813049},{"id":"https://openalex.org/keywords/irradiation","display_name":"irradiation","score":0.6579999923706055},{"id":"https://openalex.org/keywords/inner-cell-mass","display_name":"inner cell mass","score":0.8149999976158142},{"id":"https://openalex.org/keywords/cell-isolation","display_name":"cell isolation","score":0.39500001072883606}],"concepts":[{"id":"https://openalex.org/C145103041","wikidata":"https://www.wikidata.org/wiki/Q1151519","display_name":"Embryonic stem cell","level":3,"score":0.8572202920913696},{"id":"https://openalex.org/C2777164004","wikidata":"https://www.wikidata.org/wiki/Q331858","display_name":"Endoderm","level":4,"score":0.7449291348457336},{"id":"https://openalex.org/C2779329299","wikidata":"https://www.wikidata.org/wiki/Q272474","display_name":"Ectoderm","level":4,"score":0.7445993423461914},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.7370350956916809},{"id":"https://openalex.org/C18155467","wikidata":"https://www.wikidata.org/wiki/Q660498","display_name":"Germ layer","level":5,"score":0.7131107449531555},{"id":"https://openalex.org/C2780915607","wikidata":"https://www.wikidata.org/wiki/Q323438","display_name":"Mesoderm","level":4,"score":0.6585828065872192},{"id":"https://openalex.org/C23416140","wikidata":"https://www.wikidata.org/wiki/Q6335239","display_name":"KOSR","level":5,"score":0.6178402900695801},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5199198126792908},{"id":"https://openalex.org/C143948831","wikidata":"https://www.wikidata.org/wiki/Q3384920","display_name":"Embryoid body","level":5,"score":0.5014591217041016},{"id":"https://openalex.org/C107459253","wikidata":"https://www.wikidata.org/wiki/Q674925","display_name":"Induced pluripotent stem cell","level":4,"score":0.48451322317123413},{"id":"https://openalex.org/C28328180","wikidata":"https://www.wikidata.org/wiki/Q48196","display_name":"Stem cell","level":2,"score":0.47508421540260315},{"id":"https://openalex.org/C196843134","wikidata":"https://www.wikidata.org/wiki/Q33196","display_name":"Embryo","level":2,"score":0.3362312316894531},{"id":"https://openalex.org/C87073359","wikidata":"https://www.wikidata.org/wiki/Q33196","display_name":"Embryogenesis","level":3,"score":0.2725965976715088},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.15894314646720886},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.1101/pdb.prot5041","doi":"https://doi.org/10.1101/pdb.prot5041","is_oa":false,"landing_page_url":"https://doi.org/10.1101/pdb.prot5041","pdf_url":null,"source":{"id":"https://openalex.org/S119953358","display_name":"Cold Spring Harbor Protocols","issn_l":"1559-6095","issn":["1559-6095","1940-3402"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315909","host_organization_name":"Cold Spring Harbor Laboratory Press","host_organization_lineage":["https://openalex.org/P4310315909"],"host_organization_lineage_names":["Cold Spring Harbor Laboratory Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cold Spring Harbor Protocols","raw_type":"journal-article"},{"id":"pmid:21356910","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/21356910","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"CSH protocols","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.46810001134872437,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":1,"referenced_works":["https://openalex.org/W2044747743"],"related_works":["https://openalex.org/W2103478060","https://openalex.org/W2141723849","https://openalex.org/W4210987414","https://openalex.org/W2486721106","https://openalex.org/W2051472776","https://openalex.org/W2278034160","https://openalex.org/W2013127588","https://openalex.org/W4211043233","https://openalex.org/W2155603410","https://openalex.org/W3158500125"],"abstract_inverted_index":{"INTRODUCTIONEmbryonic":[0],"stem":[1],"cells":[2,50,105],"(ESCs)":[3],"are":[4,16,21,51],"derived":[5],"from":[6,108],"the":[7,29,42,65,85,102],"inner":[8],"cell":[9],"mass":[10],"of":[11,28,45,87,120],"day":[12],"5-6":[13],"blastocysts.":[14],"ESCs":[15,47],"pluripotent,":[17],"meaning":[18],"that":[19,94],"they":[20],"able":[22],"to":[23,40,53],"differentiate":[24],"into":[25],"all":[26],"derivatives":[27],"three":[30],"primary":[31],"germ":[32],"layers":[33],"(ectoderm,":[34],"endoderm,":[35],"and":[36,60,118],"mesoderm).":[37],"In":[38],"order":[39],"maintain":[41],"undifferentiated":[43,88],"status":[44],"human":[46,103],"(hESCs),":[48],"feeder":[49,104],"used":[52,82],"provide":[54],"both":[55],"a":[56,114],"suitable":[57],"attachment":[58],"substrate":[59],"critical":[61],"soluble":[62],"factors.":[63],"Since":[64],"first":[66],"hESC":[67,98,125],"lines":[68],"were":[69],"established":[70],"on":[71],"mouse":[72],"embryonic":[73],"fibroblasts":[74],"(MEFs),":[75],"mitotically":[76],"inactivated":[77],"MEFs":[78,95,121],"have":[79],"commonly":[80],"been":[81],"for":[83,116,122],"supporting":[84],"culture":[86],"hESCs.":[89],"Some":[90],"previous":[91],"studies":[92],"suggest":[93],"may":[96],"support":[97],"growth":[99],"better":[100],"than":[101],"typically":[106],"isolated":[107],"post-natal":[109],"tissues.":[110],"This":[111],"protocol":[112],"describes":[113],"method":[115],"isolation":[117],"irradiation":[119],"use":[123],"in":[124],"culture.":[126]},"counts_by_year":[{"year":2023,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":4}],"updated_date":"2026-10-30T12:39:25.321706","created_date":"2016-06-24T00:00:00"}
