{"id":"https://openalex.org/W2160249463","doi":"https://doi.org/10.1128/jvi.71.5.3799-3810.1997","title":"Engineering of homologous recombination hotspots with AU-rich sequences in brome mosaic virus","display_name":"Engineering of homologous recombination hotspots with AU-rich sequences in brome mosaic virus","publication_year":1997,"publication_date":"1997-05-01","ids":{"openalex":"https://openalex.org/W2160249463","doi":"https://doi.org/10.1128/jvi.71.5.3799-3810.1997","mag":"2160249463","pmid":"https://pubmed.ncbi.nlm.nih.gov/9094655","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/191530"},"language":"en","primary_location":{"id":"doi:10.1128/jvi.71.5.3799-3810.1997","doi":"https://doi.org/10.1128/jvi.71.5.3799-3810.1997","is_oa":false,"landing_page_url":"https://doi.org/10.1128/jvi.71.5.3799-3810.1997","pdf_url":null,"source":{"id":"https://openalex.org/S96615428","display_name":"Journal of Virology","issn_l":"0022-538X","issn":["0022-538X","1070-6321","1098-5514"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Virology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/191530","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5090380640","display_name":"Peter D. Nagy","orcid":"https://orcid.org/0000-0003-4164-1237","observed_orcids":["https://orcid.org/0000-0003-4164-1237"]},"institutions":[{"id":"https://openalex.org/I102502594","display_name":"Northern Illinois University","ror":"https://ror.org/012wxa772","country_code":"US","type":"education","lineage":["https://openalex.org/I102502594"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"P D Nagy","raw_affiliation_strings":["Department of Biological Sciences, Northern Illinois University, De Kalb 60115, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological Sciences, Northern Illinois University, De Kalb 60115, USA","institution_ids":["https://openalex.org/I102502594"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5061152927","display_name":"J\u00f3zef J. Bujarski","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I102502594","display_name":"Northern Illinois University","ror":"https://ror.org/012wxa772","country_code":"US","type":"education","lineage":["https://openalex.org/I102502594"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"J J Bujarski","raw_affiliation_strings":["Department of Biological Sciences, Northern Illinois University, De Kalb 60115, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological Sciences, Northern Illinois University, De Kalb 60115, USA","institution_ids":["https://openalex.org/I102502594"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I102502594"],"apc_list":null,"apc_paid":null,"fwci":8.6224,"has_fulltext":false,"cited_by_count":98,"citation_normalized_percentile":{"value":0.97812631,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"71","issue":"5","first_page":"3799","last_page":"3810"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10494","display_name":"Plant Virus Research Studies","score":0.9883000254631042,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10494","display_name":"Plant Virus Research Studies","score":0.9883000254631042,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11764","display_name":"Evolution and Genetic Dynamics","score":0.0013000000035390258,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13220","display_name":"Plant and Fungal Interactions Research","score":0.0012000000569969416,"subfield":{"id":"https://openalex.org/subfields/1310","display_name":"Endocrinology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/brome-mosaic-virus","display_name":"brome mosaic virus","score":0.8870000243186951},{"id":"https://openalex.org/keywords/homologous-recombination","display_name":"homologous recombination","score":0.8550000190734863},{"id":"https://openalex.org/keywords/au-rich-elements","display_name":"AU-rich elements","score":0.9710000157356262},{"id":"https://openalex.org/keywords/recombination-hotspots","display_name":"recombination hotspots","score":0.890999972820282},{"id":"https://openalex.org/keywords/rna-recombination","display_name":"RNA recombination","score":0.5590000152587891},{"id":"https://openalex.org/keywords/crossover-sites","display_name":"crossover sites","score":0.5609999895095825},{"id":"https://openalex.org/keywords/gc-content","display_name":"GC content","score":0.6340000033378601},{"id":"https://openalex.org/keywords/3-noncoding-region","display_name":"3' non-coding region","score":0.9240000247955322},{"id":"https://openalex.org/keywords/rna2","display_name":"RNA2","score":0.3959999978542328},{"id":"https://openalex.org/keywords/rna3","display_name":"RNA3","score":0.9990000128746033}],"concepts":[{"id":"https://openalex.org/C2779535198","wikidata":"https://www.wikidata.org/wiki/Q4163153","display_name":"Brome mosaic virus","level":5,"score":0.928688108921051},{"id":"https://openalex.org/C102744134","wikidata":"https://www.wikidata.org/wiki/Q22280388","display_name":"Homologous recombination","level":3,"score":0.8035876154899597},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7971000671386719},{"id":"https://openalex.org/C156695909","wikidata":"https://www.wikidata.org/wiki/Q3373825","display_name":"Recombination","level":3,"score":0.7965934872627258},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.6728659868240356},{"id":"https://openalex.org/C64894306","wikidata":"https://www.wikidata.org/wiki/Q849622","display_name":"Homologous chromosome","level":3,"score":0.6511458158493042},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.6074853539466858},{"id":"https://openalex.org/C50919411","wikidata":"https://www.wikidata.org/wiki/Q5334294","display_name":"Ectopic recombination","level":5,"score":0.5550975203514099},{"id":"https://openalex.org/C82040048","wikidata":"https://www.wikidata.org/wiki/Q7049125","display_name":"Non-allelic homologous recombination","level":5,"score":0.5240381360054016},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.33517658710479736},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3206822872161865},{"id":"https://openalex.org/C41258723","wikidata":"https://www.wikidata.org/wiki/Q2919111","display_name":"RNA-dependent RNA polymerase","level":4,"score":0.30436789989471436},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.28788959980010986},{"id":"https://openalex.org/C101434241","wikidata":"https://www.wikidata.org/wiki/Q211675","display_name":"Genetic recombination","level":4,"score":0.2717917561531067}],"mesh":[{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011995","descriptor_name":"Recombination, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012367","descriptor_name":"RNA, Viral","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D017795","descriptor_name":"Bromovirus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1128/jvi.71.5.3799-3810.1997","doi":"https://doi.org/10.1128/jvi.71.5.3799-3810.1997","is_oa":false,"landing_page_url":"https://doi.org/10.1128/jvi.71.5.3799-3810.1997","pdf_url":null,"source":{"id":"https://openalex.org/S96615428","display_name":"Journal of Virology","issn_l":"0022-538X","issn":["0022-538X","1070-6321","1098-5514"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Virology","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:191530","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/191530","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Virol","raw_type":"Text"},{"id":"pmid:9094655","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9094655","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of virology","raw_type":"Journal Article"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:191530","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/191530","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Virol","raw_type":"Text"},"sustainable_development_goals":[],"sustainable_development_goals_aurora":[{"id":"https://metadata.un.org/sdg/15","display_name":"Life on land","score":0.5}],"study_designs":[],"awards":[{"id":"https://openalex.org/G134313835","display_name":null,"funder_award_id":"3RO1 AI26769","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"},{"id":"https://openalex.org/G439582586","display_name":"RECOMBINATION IN THE MULTIPARTITE RNA VIRUSES","funder_award_id":"5r01ai026769-05","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":39,"referenced_works":["https://openalex.org/W9694778","https://openalex.org/W44757669","https://openalex.org/W1485913963","https://openalex.org/W1490300695","https://openalex.org/W1507445211","https://openalex.org/W1537486010","https://openalex.org/W1580027475","https://openalex.org/W1581733591","https://openalex.org/W1589329533","https://openalex.org/W1693269273","https://openalex.org/W1791703808","https://openalex.org/W1832347372","https://openalex.org/W1867480059","https://openalex.org/W1917399449","https://openalex.org/W1963620791","https://openalex.org/W1979608173","https://openalex.org/W1988411415","https://openalex.org/W1997866518","https://openalex.org/W2001680192","https://openalex.org/W2005402262","https://openalex.org/W2007794046","https://openalex.org/W2029816142","https://openalex.org/W2037720398","https://openalex.org/W2043720462","https://openalex.org/W2056363020","https://openalex.org/W2060190614","https://openalex.org/W2064475194","https://openalex.org/W2071944390","https://openalex.org/W2072883428","https://openalex.org/W2075704510","https://openalex.org/W2079936170","https://openalex.org/W2112775060","https://openalex.org/W2118451774","https://openalex.org/W2125107956","https://openalex.org/W2140301907","https://openalex.org/W2144067328","https://openalex.org/W2144805902","https://openalex.org/W2154632774","https://openalex.org/W2185291440"],"related_works":["https://openalex.org/W2092410421","https://openalex.org/W2133255274","https://openalex.org/W1791703808","https://openalex.org/W2087014137","https://openalex.org/W2164442229","https://openalex.org/W2144805902","https://openalex.org/W9690612","https://openalex.org/W1991308137","https://openalex.org/W2107858134","https://openalex.org/W1212495428"],"abstract_inverted_index":{"Previously,":[0],"we":[1],"observed":[2,160],"that":[3,115,136,145],"crossovers":[4],"sites":[5,139],"of":[6,86,121,140,151],"RNA":[7,91,101,153],"recombinants":[8],"clustered":[9],"within":[10,107],"or":[11,78,100,125],"close":[12],"to":[13,157],"AU-rich":[14,38,44,65,88,127,130],"regions":[15],"during":[16,149],"genetic":[17],"recombination":[18,61,71,92],"in":[19],"brome":[20],"mosaic":[21],"bromovirus":[22],"(BMV)":[23],"(P.":[24],"D.":[25],"Nagy":[26],"and":[27,53,103,128],"J.":[28,29,31],"Bujarski.":[30],"Virol.":[32],"70:415-426,":[33],"1996).":[34],"To":[35],"test":[36],"whether":[37],"sequences":[39,45,66],"can":[40],"facilitate":[41],"homologous":[42,59,70,141],"recombination,":[43],"were":[46],"introduced":[47],"into":[48],"parental":[49],"BMV":[50],"RNAs":[51],"(RNA2":[52],"RNA3).":[54],"These":[55,112],"insertions":[56],"created":[57],"a":[58,73],"RNA2-RNA3":[60],"hotspot.":[62],"Two":[63],"other":[64],"also":[67],"supported":[68],"high-frequency":[69],"if":[72],"common":[74,122],"sequence":[75,98],"with":[76],"high":[77],"average":[79],"G/C":[80],"content":[81,117],"was":[82,104],"present":[83],"immediately":[84],"upstream":[85],"the":[87,108,119,133,138,159],"element.":[89],"Homologous":[90],"did":[93],"not":[94],"require":[95],"any":[96],"additional":[97],"motifs":[99],"structures":[102],"position":[105],"nonspecific":[106],"3'":[109,129],"noncoding":[110],"region.":[111],"results":[113],"suggest":[114],"nucleotide":[116],"(i.e.,":[118],"presence":[120],"5'":[123],"GC-rich":[124],"moderately":[126],"regions)":[131],"is":[132,155],"important":[134],"factor":[135],"determines":[137],"recombination.":[142],"A":[143],"mechanism":[144],"involves":[146],"replicase":[147],"switching":[148],"synthesis":[150],"positive-sense":[152],"strands":[154],"presented":[156],"explain":[158],"results.":[161]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":6},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":4},{"year":2017,"cited_by_count":4},{"year":2015,"cited_by_count":2},{"year":2013,"cited_by_count":5},{"year":2012,"cited_by_count":4}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}