{"id":"https://openalex.org/W2159790210","doi":"https://doi.org/10.1021/bp049644r","title":"Development of a Purification Process for Adenovirus: Controlling Virus Aggregation to Improve the Clearance of Host Cell DNA","display_name":"Development of a Purification Process for Adenovirus: Controlling Virus Aggregation to Improve the Clearance of Host Cell DNA","publication_year":2005,"publication_date":"2005-04-01","ids":{"openalex":"https://openalex.org/W2159790210","doi":"https://doi.org/10.1021/bp049644r","mag":"2159790210","pmid":"https://pubmed.ncbi.nlm.nih.gov/15801787"},"language":"en","primary_location":{"id":"doi:10.1021/bp049644r","doi":"https://doi.org/10.1021/bp049644r","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bp049644r","pdf_url":null,"source":{"id":"https://openalex.org/S186451451","display_name":"Biotechnology Progress","issn_l":"1520-6033","issn":["1520-6033","8756-7938"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology Progress","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5085074509","display_name":"John Konz","orcid":"https://orcid.org/0000-0001-8309-5540","observed_orcids":["https://orcid.org/0000-0001-8309-5540"]},"institutions":[{"id":"https://openalex.org/I1285764155","display_name":"Merck & Co., Inc., Rahway, NJ, USA (United States)","ror":"https://ror.org/02891sr49","country_code":"US","type":"company","lineage":["https://openalex.org/I1285764155"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"John O. Konz","raw_affiliation_strings":["Biologics Development & Engineering and Live Viral Vectors, BioProcess R&D, Merck Research Laboratories, West Point, Pennsylvania 19486, USA. john_konz@merck.com","Biologics Development & Engineering and Live Viral Vectors, BioProcess R&D, Merck Research Laboratories, West Point, Pennsylvania 19486"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biologics Development & Engineering and Live Viral Vectors, BioProcess R&D, Merck Research Laboratories, West Point, Pennsylvania 19486, USA. john_konz@merck.com","institution_ids":["https://openalex.org/I1285764155"]},{"raw_affiliation_string":"Biologics Development & Engineering and Live Viral Vectors, BioProcess R&D, Merck Research Laboratories, West Point, Pennsylvania 19486","institution_ids":["https://openalex.org/I1285764155"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5011502237","display_name":"Ann L. Lee","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1285764155","display_name":"Merck & Co., Inc., Rahway, NJ, USA (United States)","ror":"https://ror.org/02891sr49","country_code":"US","type":"company","lineage":["https://openalex.org/I1285764155"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ann L. Lee","raw_affiliation_strings":["Biologics Development & Engineering and Live Viral Vectors, BioProcess R&D, Merck Research Laboratories, West Point, Pennsylvania 19486"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biologics Development & Engineering and Live Viral Vectors, BioProcess R&D, Merck Research Laboratories, West Point, Pennsylvania 19486","institution_ids":["https://openalex.org/I1285764155"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5029935997","display_name":"John A. Lewis","orcid":"https://orcid.org/0000-0002-3992-7837","observed_orcids":["https://orcid.org/0000-0002-3992-7837"]},"institutions":[{"id":"https://openalex.org/I1285764155","display_name":"Merck & Co., Inc., Rahway, NJ, USA (United States)","ror":"https://ror.org/02891sr49","country_code":"US","type":"company","lineage":["https://openalex.org/I1285764155"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"John A. Lewis","raw_affiliation_strings":["Biologics Development & Engineering and Live Viral Vectors, BioProcess R&D, Merck Research Laboratories, West Point, Pennsylvania 19486","Live Viral Vectors, BioProcess R&D"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biologics Development & Engineering and Live Viral Vectors, BioProcess R&D, Merck Research Laboratories, West Point, Pennsylvania 19486","institution_ids":["https://openalex.org/I1285764155"]},{"raw_affiliation_string":"Live Viral Vectors, BioProcess R&D","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5016944934","display_name":"Sangeetha L. Sagar","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1285764155","display_name":"Merck & Co., Inc., Rahway, NJ, USA (United States)","ror":"https://ror.org/02891sr49","country_code":"US","type":"company","lineage":["https://openalex.org/I1285764155"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Sangeetha L. Sagar","raw_affiliation_strings":["Biologics Development & Engineering and Live Viral Vectors, BioProcess R&D, Merck Research Laboratories, West Point, Pennsylvania 19486"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biologics Development & Engineering and Live Viral Vectors, BioProcess R&D, Merck Research Laboratories, West Point, Pennsylvania 19486","institution_ids":["https://openalex.org/I1285764155"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5085074509"],"corresponding_institution_ids":["https://openalex.org/I1285764155"],"apc_list":{"value":4160,"currency":"USD","value_usd":4160},"apc_paid":null,"fwci":1.5505,"has_fulltext":false,"cited_by_count":72,"citation_normalized_percentile":{"value":0.79758022,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"21","issue":"2","first_page":"466","last_page":"472"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.9678000211715698,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.9678000211715698,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.021299999207258224,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.00860000029206276,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/virus-aggregation","display_name":"virus aggregation","score":0.8299999833106995},{"id":"https://openalex.org/keywords/recombinant-adenovirus-type-5","display_name":"recombinant adenovirus type 5","score":0.8429999947547913},{"id":"https://openalex.org/keywords/nuclease-digestion","display_name":"nuclease digestion","score":0.7670000195503235},{"id":"https://openalex.org/keywords/anion-exchange-chromatography","display_name":"anion exchange chromatography","score":0.8880000114440918},{"id":"https://openalex.org/keywords/ultrafiltration","display_name":"ultrafiltration","score":0.9559999704360962},{"id":"https://openalex.org/keywords/hydroxyapatite-chromatography","display_name":"hydroxyapatite chromatography","score":0.9279999732971191},{"id":"https://openalex.org/keywords/nonionic-detergents","display_name":"nonionic detergents","score":0.9440000057220459},{"id":"https://openalex.org/keywords/electrostatic-interactions","display_name":"electrostatic interactions","score":0.7770000100135803},{"id":"https://openalex.org/keywords/hydrophobic-interactions","display_name":"hydrophobic interactions","score":0.9139999747276306},{"id":"https://openalex.org/keywords/viral-vectors","display_name":"viral vectors","score":0.3630000054836273},{"id":"https://openalex.org/keywords/gene-therapy-vectors","display_name":"gene therapy vectors","score":0.7749999761581421},{"id":"https://openalex.org/keywords/vaccine-vectors","display_name":"vaccine vectors","score":0.597000002861023}],"concepts":[{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.7525832056999207},{"id":"https://openalex.org/C2777271071","wikidata":"https://www.wikidata.org/wiki/Q422529","display_name":"Nuclease","level":3,"score":0.6913121938705444},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.671007513999939},{"id":"https://openalex.org/C50817676","wikidata":"https://www.wikidata.org/wiki/Q7880534","display_name":"Ultrafiltration (renal)","level":2,"score":0.6089377403259277},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5998249053955078},{"id":"https://openalex.org/C139024627","wikidata":"https://www.wikidata.org/wiki/Q4293132","display_name":"Micrococcal nuclease","level":5,"score":0.5802686214447021},{"id":"https://openalex.org/C57409179","wikidata":"https://www.wikidata.org/wiki/Q1218293","display_name":"Lysis","level":2,"score":0.5449702739715576},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.505298912525177},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.49895572662353516},{"id":"https://openalex.org/C2908647359","wikidata":"https://www.wikidata.org/wiki/Q2625603","display_name":"Population","level":2,"score":0.487544983625412},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.41558313369750977},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.37581542134284973},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2857424020767212},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.24936243891716003},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.16563725471496582},{"id":"https://openalex.org/C84772758","wikidata":"https://www.wikidata.org/wiki/Q271794","display_name":"Nucleosome","level":4,"score":0.0},{"id":"https://openalex.org/C144024400","wikidata":"https://www.wikidata.org/wiki/Q21201","display_name":"Sociology","level":0,"score":0.0},{"id":"https://openalex.org/C64927066","wikidata":"https://www.wikidata.org/wiki/Q36293","display_name":"Histone","level":3,"score":0.0},{"id":"https://openalex.org/C149923435","wikidata":"https://www.wikidata.org/wiki/Q37732","display_name":"Demography","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000256","descriptor_name":"Adenoviridae","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D000837","descriptor_name":"Anion Exchange Resins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002851","descriptor_name":"Chromatography, High Pressure Liquid","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D002852","descriptor_name":"Chromatography, Ion Exchange","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D004591","descriptor_name":"Electrophoresis, Polyacrylamide Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011995","descriptor_name":"Recombination, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1021/bp049644r","doi":"https://doi.org/10.1021/bp049644r","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bp049644r","pdf_url":null,"source":{"id":"https://openalex.org/S186451451","display_name":"Biotechnology Progress","issn_l":"1520-6033","issn":["1520-6033","8756-7938"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology Progress","raw_type":"journal-article"},{"id":"pmid:15801787","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/15801787","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology progress","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.5683000087738037,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":30,"referenced_works":["https://openalex.org/W16795284","https://openalex.org/W62943476","https://openalex.org/W1505035325","https://openalex.org/W1968567648","https://openalex.org/W1973905531","https://openalex.org/W1979878725","https://openalex.org/W1988597673","https://openalex.org/W2005207212","https://openalex.org/W2009109390","https://openalex.org/W2020405271","https://openalex.org/W2027210894","https://openalex.org/W2029900373","https://openalex.org/W2037886909","https://openalex.org/W2041195152","https://openalex.org/W2044296969","https://openalex.org/W2046306990","https://openalex.org/W2054794512","https://openalex.org/W2064500738","https://openalex.org/W2089965834","https://openalex.org/W2102084617","https://openalex.org/W2141866540","https://openalex.org/W2154309992","https://openalex.org/W2169702122","https://openalex.org/W2173735446","https://openalex.org/W2322389503","https://openalex.org/W2530364790","https://openalex.org/W2991681422","https://openalex.org/W2992021843","https://openalex.org/W4285719527","https://openalex.org/W4318443595"],"related_works":["https://openalex.org/W1999193101","https://openalex.org/W4246389566","https://openalex.org/W2063084168","https://openalex.org/W1990401827","https://openalex.org/W3047386023","https://openalex.org/W2025818017","https://openalex.org/W2038696480","https://openalex.org/W4389452516","https://openalex.org/W3010933045","https://openalex.org/W2142032603"],"abstract_inverted_index":{"The":[0],"clearance":[1,28,168],"of":[2,29,57,64,96,103,131,165],"host":[3,48,141],"cell":[4,49,142],"DNA":[5,30,50,87,143,159,167,174],"is":[6],"a":[7,32,54,94,101],"critical":[8],"goal":[9],"for":[10,14],"purification":[11,34],"process":[12,35,135,166],"development":[13],"recombinant":[15],"Ad5":[16],"(rAd5)":[17],"based":[18],"vaccines":[19],"and":[20,40,45,79,122,160,170],"gene":[21],"therapy":[22],"products.":[23],"We":[24],"have":[25],"evaluated":[26],"the":[27,65,85,134,172],"by":[31],"rAd5":[33,61,67,104,151,161],"utilizing":[36],"nuclease":[37,76],"digestion,":[38],"ultrafiltration,":[39],"anion":[41],"exchange":[42],"(AEX)":[43],"chromatography":[44],"found":[46],"residual":[47,86],"to":[51,118,125,138,144],"consistently":[52,139],"reach":[53],"limiting":[55],"value":[56],"about":[58],"100":[59],"pg/10(11)":[60,150],"particles.":[62,92],"Characterization":[63],"purified":[66],"product":[68],"using":[69,115],"serial":[70],"AEX":[71],"chromatography,":[72,74],"hydroxyapatite":[73],"or":[75,147],"treatment":[77],"with":[78,90],"without":[80],"particle":[81],"disruption":[82],"showed":[83],"that":[84,154,171],"was":[88,110,113,136,175],"associated":[89],"virus":[91,105],"Using":[93],"variety":[95],"additional":[97],"physical":[98],"characterization":[99],"methods,":[100],"population":[102],"in":[106],"an":[107],"aggregated":[108],"state":[109],"detected.":[111],"Aggregation":[112],"eliminated":[114],"nonionic":[116],"detergents":[117],"attenuate":[119,126],"hydrophobic":[120],"interactions":[121,156],"sodium":[123],"chloride":[124],"electrostatic":[127],"interactions.":[128],"After":[129],"implementation":[130],"these":[132],"modifications,":[133],"able":[137],"reduce":[140],"levels":[145],"at":[146],"below":[148],"5":[149],"particles,":[152],"suggesting":[153],"molecular":[155],"between":[157],"cellular":[158],"are":[162],"important":[163],"determinants":[164],"capability":[169],"co-purifying":[173],"not":[176],"encapsidated.":[177]},"counts_by_year":[{"year":2025,"cited_by_count":3},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":5},{"year":2021,"cited_by_count":5},{"year":2020,"cited_by_count":7},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":4},{"year":2016,"cited_by_count":3},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":8},{"year":2013,"cited_by_count":3},{"year":2012,"cited_by_count":2}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}