{"id":"https://openalex.org/W2159284166","doi":"https://doi.org/10.1021/bc960055j","title":"Improved Characteristics of a Human \u03b2-Glucuronidase\u2212Antibody Conjugate after Deglycosylation for Use in Antibody-Directed Enzyme Prodrug Therapy","display_name":"Improved Characteristics of a Human \u03b2-Glucuronidase\u2212Antibody Conjugate after Deglycosylation for Use in Antibody-Directed Enzyme Prodrug Therapy","publication_year":1996,"publication_date":"1996-01-01","ids":{"openalex":"https://openalex.org/W2159284166","doi":"https://doi.org/10.1021/bc960055j","mag":"2159284166","pmid":"https://pubmed.ncbi.nlm.nih.gov/8889024"},"language":"en","primary_location":{"id":"doi:10.1021/bc960055j","doi":"https://doi.org/10.1021/bc960055j","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bc960055j","pdf_url":null,"source":{"id":"https://openalex.org/S155115410","display_name":"Bioconjugate Chemistry","issn_l":"1043-1802","issn":["1043-1802","1520-4812"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioconjugate Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5113561493","display_name":"Pieter H.J. Houba","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I865915315","display_name":"Vrije Universiteit Amsterdam","ror":"https://ror.org/008xxew50","country_code":"NL","type":"education","lineage":["https://openalex.org/I865915315"]},{"id":"https://openalex.org/I911458345","display_name":"Amsterdam UMC Location Vrije Universiteit Amsterdam","ror":"https://ror.org/00q6h8f30","country_code":"NL","type":"healthcare","lineage":["https://openalex.org/I4210151833","https://openalex.org/I911458345"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"Pieter H. J. Houba","raw_affiliation_strings":["Department of Medical Oncology, Academic Hospital Vrije Universiteit, De Boelelaan 1117, 1081\u2009HV Amsterdam, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medical Oncology, Academic Hospital Vrije Universiteit, De Boelelaan 1117, 1081\u2009HV Amsterdam, The Netherlands","institution_ids":["https://openalex.org/I865915315","https://openalex.org/I911458345"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5088663155","display_name":"Epie Boven","orcid":"https://orcid.org/0000-0001-9287-743X","observed_orcids":["https://orcid.org/0000-0001-9287-743X"]},"institutions":[{"id":"https://openalex.org/I865915315","display_name":"Vrije Universiteit Amsterdam","ror":"https://ror.org/008xxew50","country_code":"NL","type":"education","lineage":["https://openalex.org/I865915315"]},{"id":"https://openalex.org/I911458345","display_name":"Amsterdam UMC Location Vrije Universiteit Amsterdam","ror":"https://ror.org/00q6h8f30","country_code":"NL","type":"healthcare","lineage":["https://openalex.org/I4210151833","https://openalex.org/I911458345"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"Epie Boven","raw_affiliation_strings":["Department of Medical Oncology, Academic Hospital Vrije Universiteit, De Boelelaan 1117, 1081\u2009HV Amsterdam, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medical Oncology, Academic Hospital Vrije Universiteit, De Boelelaan 1117, 1081\u2009HV Amsterdam, The Netherlands","institution_ids":["https://openalex.org/I865915315","https://openalex.org/I911458345"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5021729725","display_name":"Hidde J. Haisma","orcid":"https://orcid.org/0000-0003-3997-9052","observed_orcids":["https://orcid.org/0000-0003-3997-9052"]},"institutions":[{"id":"https://openalex.org/I865915315","display_name":"Vrije Universiteit Amsterdam","ror":"https://ror.org/008xxew50","country_code":"NL","type":"education","lineage":["https://openalex.org/I865915315"]},{"id":"https://openalex.org/I911458345","display_name":"Amsterdam UMC Location Vrije Universiteit Amsterdam","ror":"https://ror.org/00q6h8f30","country_code":"NL","type":"healthcare","lineage":["https://openalex.org/I4210151833","https://openalex.org/I911458345"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"Hidde J. Haisma","raw_affiliation_strings":["Department of Medical Oncology, Academic Hospital Vrije Universiteit, De Boelelaan 1117, 1081\u2009HV Amsterdam, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medical Oncology, Academic Hospital Vrije Universiteit, De Boelelaan 1117, 1081\u2009HV Amsterdam, The Netherlands","institution_ids":["https://openalex.org/I865915315","https://openalex.org/I911458345"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":1.0175,"has_fulltext":false,"cited_by_count":25,"citation_normalized_percentile":{"value":0.77004011,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":95},"biblio":{"volume":"7","issue":"5","first_page":"606","last_page":"611"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.6187000274658203,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.6187000274658203,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12477","display_name":"Toxin Mechanisms and Immunotoxins","score":0.15760000050067902,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.08060000091791153,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/adept","display_name":"ADEPT","score":0.9860000014305115},{"id":"https://openalex.org/keywords/deglycosylation","display_name":"deglycosylation","score":0.859000027179718},{"id":"https://openalex.org/keywords/enzyme-glycosylation","display_name":"enzyme glycosylation","score":0.5090000033378601},{"id":"https://openalex.org/keywords/hepatic-clearance","display_name":"hepatic clearance","score":0.8190000057220459},{"id":"https://openalex.org/keywords/prodrug-activation","display_name":"prodrug activation","score":0.6610000133514404},{"id":"https://openalex.org/keywords/tumor-to-blood-ratio","display_name":"tumor-to-blood ratio","score":0.6549999713897705},{"id":"https://openalex.org/keywords/tumor-localization","display_name":"tumor localization","score":0.45500001311302185}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.8827590942382812},{"id":"https://openalex.org/C2778556491","wikidata":"https://www.wikidata.org/wiki/Q1660173","display_name":"Immunoconjugate","level":4,"score":0.8730125427246094},{"id":"https://openalex.org/C108215921","wikidata":"https://www.wikidata.org/wiki/Q603741","display_name":"Prodrug","level":2,"score":0.8696187138557434},{"id":"https://openalex.org/C197336794","wikidata":"https://www.wikidata.org/wiki/Q5161150","display_name":"Conjugate","level":2,"score":0.866085410118103},{"id":"https://openalex.org/C2776904630","wikidata":"https://www.wikidata.org/wiki/Q356336","display_name":"Adept","level":3,"score":0.8539503812789917},{"id":"https://openalex.org/C207001950","wikidata":"https://www.wikidata.org/wiki/Q141124","display_name":"In vivo","level":2,"score":0.6714255213737488},{"id":"https://openalex.org/C542903549","wikidata":"https://www.wikidata.org/wiki/Q422248","display_name":"Monoclonal antibody","level":3,"score":0.6326904892921448},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.6191610097885132},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.6168797016143799},{"id":"https://openalex.org/C2780868878","wikidata":"https://www.wikidata.org/wiki/Q1660197","display_name":"Immunogenicity","level":3,"score":0.47375398874282837},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.4370180666446686},{"id":"https://openalex.org/C98274493","wikidata":"https://www.wikidata.org/wiki/Q128406","display_name":"Pharmacology","level":1,"score":0.41846251487731934},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.38613802194595337},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3760315477848053},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.12735995650291443},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.0},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.0},{"id":"https://openalex.org/C134306372","wikidata":"https://www.wikidata.org/wiki/Q7754","display_name":"Mathematical analysis","level":1,"score":0.0},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.0},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000906","descriptor_name":"Antibodies","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005966","descriptor_name":"Glucuronidase","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D006031","descriptor_name":"Glycosylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008807","descriptor_name":"Mice, Inbred BALB C","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010051","descriptor_name":"Ovarian Neoplasms","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D014407","descriptor_name":"Tumor Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016503","descriptor_name":"Drug Delivery Systems","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1021/bc960055j","doi":"https://doi.org/10.1021/bc960055j","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bc960055j","pdf_url":null,"source":{"id":"https://openalex.org/S155115410","display_name":"Bioconjugate Chemistry","issn_l":"1043-1802","issn":["1043-1802","1520-4812"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioconjugate Chemistry","raw_type":"journal-article"},{"id":"pmid:8889024","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8889024","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioconjugate chemistry","raw_type":"Journal Article"},{"id":"pmh:oai:pure.rug.nl:openaire_cris_publications/a0bd2048-1700-45a8-91cb-a855947993c0","doi":null,"is_oa":false,"landing_page_url":"https://hdl.handle.net/11370/a0bd2048-1700-45a8-91cb-a855947993c0","pdf_url":null,"source":{"id":"https://openalex.org/S4306400420","display_name":"University of Groningen research database (University of Groningen / Centre for Information Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I169381384","host_organization_name":"University of Groningen","host_organization_lineage":["https://openalex.org/I169381384"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Houba, PHJ, Boven, E & Haisma, HJ 1996, 'Improved characteristics of a human beta-glucuronidase-antibody conjugate after deglycosylation for use in antibody-directed enzyme prodrug therapy', Bioconjugate Chemistry, vol. 7, no. 5, pp. 606-611.","raw_type":"info:eu-repo/semantics/article"},{"id":"pmh:rug:oai:pure.rug.nl:publications/a0bd2048-1700-45a8-91cb-a855947993c0","doi":null,"is_oa":false,"landing_page_url":"https://research.rug.nl/en/publications/a0bd2048-1700-45a8-91cb-a855947993c0","pdf_url":null,"source":{"id":"https://openalex.org/S4306400420","display_name":"University of Groningen research database (University of Groningen / Centre for Information Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I169381384","host_organization_name":"University of Groningen","host_organization_lineage":["https://openalex.org/I169381384"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"BIOCONJUGATE CHEMISTRY, 7(5), 606 - 611. AMER CHEMICAL SOC","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.9476000070571899}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":15,"referenced_works":["https://openalex.org/W976721469","https://openalex.org/W1526653134","https://openalex.org/W1528681389","https://openalex.org/W1580424021","https://openalex.org/W1983430641","https://openalex.org/W2008932642","https://openalex.org/W2022770804","https://openalex.org/W2029326803","https://openalex.org/W2058426435","https://openalex.org/W2074011524","https://openalex.org/W2116136455","https://openalex.org/W2465223718","https://openalex.org/W2949075511","https://openalex.org/W4255006975","https://openalex.org/W4300919850"],"related_works":["https://openalex.org/W2079185876","https://openalex.org/W1979208227","https://openalex.org/W2090096034","https://openalex.org/W2027237423","https://openalex.org/W2320091911","https://openalex.org/W1987065191","https://openalex.org/W2124409897","https://openalex.org/W1972893947","https://openalex.org/W2063836141","https://openalex.org/W2075159432"],"abstract_inverted_index":{"Antibody-directed":[0],"enzyme":[1,88,110],"prodrug":[2,121,239],"therapy":[3],"(ADEPT)":[4],"aims":[5],"at":[6,17,211,229],"the":[7,18,23,48,78,87,108,125,132,153,175,194,227],"specific":[8,102,191],"activation":[9],"of":[10,22,50,61,82,119,134,177,184,221],"relatively":[11],"nontoxic":[12],"prodrugs":[13],"into":[14],"active":[15],"drugs":[16],"tumor":[19,115,195],"site.":[20],"One":[21],"enzymes":[24],"described":[25],"to":[26,38,73,114,182,197],"be":[27],"useful":[28],"in":[29,42,77,152,193],"ADEPT":[30],"is":[31,36,53,235],"human":[32],"beta-glucuronidase":[33],"(GUS),":[34],"which":[35,234],"expected":[37],"have":[39,63],"low":[40],"immunogenicity":[41],"patients.":[43],"A":[44,217],"major":[45],"obstacle":[46],"for":[47,226,237],"use":[49],"GUS,":[51],"however,":[52],"its":[54,75],"rapid":[55],"glycan-specific":[56],"hepatic":[57],"clearance.":[58],"The":[59,80,187],"carbohydrates":[60],"GUS":[62,83,163],"been":[64],"modified":[65,109],"by":[66],"subsequent":[67],"treatment":[68],"with":[69,99,161],"NaIO4":[70],"and":[71,107],"NaBH4":[72],"improve":[74],"retention":[76],"circulation.":[79],"modification":[81],"did":[84],"not":[85],"decrease":[86],"activity.":[89],"In":[90,123,168],"vitro":[91],"it":[92,149],"was":[93,117,128,150,179,224],"demonstrated":[94],"that":[95,183],"a":[96,100,198],"conjugate":[97,127,189,228],"prepared":[98,160],"pancarcinoma":[101],"monoclonal":[103],"antibody":[104],"(mAb)":[105],"323/A3":[106,143,203],"(mGUS),":[111],"when":[112],"bound":[113],"cells,":[116],"capable":[118],"complete":[120],"activation.":[122,240],"vivo,":[124],"323/A3-mGUS":[126,178,188],"cleared":[129],"faster":[130],"from":[131],"circulation":[133,154],"BALB/c":[135],"mice":[136,170],"(t1/2":[137,144,164],"=":[138,145,165],"9":[139],"h)":[140],"than":[141,157,201],"mAb":[142,185,202],"32":[146],"h),":[147],"but":[148,196],"retained":[151],"much":[155],"longer":[156],"an":[158],"immunoconjugate":[159],"native":[162],"24":[166],"min).":[167],"nude":[169],"bearing":[171],"subcutaneous":[172],"OVCAR-3":[173],"tumors":[174],"distribution":[176],"qualitatively":[180],"comparable":[181],"323/A3.":[186],"showed":[190],"localization":[192],"lesser":[199],"extent":[200],"(2.7%":[204],"vs":[205],"6.4%":[206],"injected":[207],"dose":[208],"per":[209],"gram":[210],"1":[212],"day":[213],"after":[214,232],"iv":[215],"injection).":[216],"favorable":[218],"tumor-to-blood":[219],"ratio":[220],">":[222],"2":[223],"observed":[225],"7":[230],"days":[231],"administration,":[233],"necessary":[236],"tumor-specific":[238]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2014,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}