{"id":"https://openalex.org/W2159168253","doi":null,"title":"Insights into enzyme-catalysed phosphoryl transfer from studies of MAP kinases","display_name":"Insights into enzyme-catalysed phosphoryl transfer from studies of MAP kinases","publication_year":2014,"publication_date":"2014-01-01","ids":{"openalex":"https://openalex.org/W2159168253","mag":"2159168253"},"language":"en","primary_location":{"id":"pmh:oai:etheses.whiterose.ac.uk:8735","doi":null,"is_oa":false,"landing_page_url":"http://etheses.whiterose.ac.uk/8735/1/COMBINED_aftercorrections_FINAL.docx","pdf_url":null,"source":{"id":"https://openalex.org/S4306401585","display_name":"White Rose eTheses Online (University of Leeds, The University of Sheffield, University of York)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2800616092","host_organization_name":"White Rose University Consortium","host_organization_lineage":["https://openalex.org/I2800616092"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://uilis.unsyiah.ac.id/opentheses/items/show/48733","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5085592378","display_name":"Thomas P. Lake","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Thomas P. Lake","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5085592378"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.7134000062942505,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.7134000062942505,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12104","display_name":"Protein Tyrosine Phosphatases","score":0.2667999863624573,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12191","display_name":"Physical Organic Chemistry and Reaction Kinetics","score":0.007799999788403511,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/phosphoryl-transfer","display_name":"phosphoryl transfer","score":0.871999979019165},{"id":"https://openalex.org/keywords/protein-kinases","display_name":"protein kinases","score":0.7910000085830688},{"id":"https://openalex.org/keywords/mitogen-activated-protein-kinase","display_name":"mitogen-activated protein kinase","score":0.7300000190734863},{"id":"https://openalex.org/keywords/p38alpha","display_name":"p38\u03b1","score":0.9290000200271606},{"id":"https://openalex.org/keywords/mek","display_name":"MEK","score":0.6330000162124634},{"id":"https://openalex.org/keywords/dual-specificity-kinase","display_name":"dual-specificity kinase","score":0.7480000257492065},{"id":"https://openalex.org/keywords/protein-phosphatase-activity","display_name":"protein phosphatase activity","score":0.8429999947547913},{"id":"https://openalex.org/keywords/dual-phosphorylation","display_name":"dual phosphorylation","score":0.6359999775886536},{"id":"https://openalex.org/keywords/activation-loop","display_name":"activation loop","score":0.8600000143051147},{"id":"https://openalex.org/keywords/general-base-catalysis","display_name":"general base catalysis","score":0.7360000014305115}],"concepts":[{"id":"https://openalex.org/C178666793","wikidata":"https://www.wikidata.org/wiki/Q422476","display_name":"Phosphatase","level":3,"score":0.7599552869796753},{"id":"https://openalex.org/C184235292","wikidata":"https://www.wikidata.org/wiki/Q421851","display_name":"Kinase","level":2,"score":0.7342928051948547},{"id":"https://openalex.org/C11960822","wikidata":"https://www.wikidata.org/wiki/Q242736","display_name":"Phosphorylation","level":2,"score":0.6358140110969543},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6304643154144287},{"id":"https://openalex.org/C2780429522","wikidata":"https://www.wikidata.org/wiki/Q83132533","display_name":"Dual-specificity phosphatase","level":4,"score":0.5409383177757263},{"id":"https://openalex.org/C54174078","wikidata":"https://www.wikidata.org/wiki/Q3197188","display_name":"Kinome","level":3,"score":0.5079212784767151},{"id":"https://openalex.org/C2910050888","wikidata":"https://www.wikidata.org/wiki/Q4857068","display_name":"Protein-Serine-Threonine Kinases","level":4,"score":0.498931884765625},{"id":"https://openalex.org/C85528070","wikidata":"https://www.wikidata.org/wiki/Q643602","display_name":"Protein tyrosine phosphatase","level":3,"score":0.4935694932937622},{"id":"https://openalex.org/C2775880066","wikidata":"https://www.wikidata.org/wiki/Q186521","display_name":"Threonine","level":4,"score":0.4853214621543884},{"id":"https://openalex.org/C132149769","wikidata":"https://www.wikidata.org/wiki/Q899651","display_name":"Mitogen-activated protein kinase","level":3,"score":0.46144288778305054},{"id":"https://openalex.org/C2776414213","wikidata":"https://www.wikidata.org/wiki/Q183290","display_name":"Serine","level":3,"score":0.45628273487091064},{"id":"https://openalex.org/C51551487","wikidata":"https://www.wikidata.org/wiki/Q2043632","display_name":"p38 mitogen-activated protein kinases","level":4,"score":0.43003910779953003},{"id":"https://openalex.org/C97029542","wikidata":"https://www.wikidata.org/wiki/Q281417","display_name":"Protein kinase A","level":3,"score":0.426480233669281},{"id":"https://openalex.org/C87325107","wikidata":"https://www.wikidata.org/wiki/Q7251493","display_name":"Protein phosphorylation","level":4,"score":0.4238136112689972},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4192378520965576},{"id":"https://openalex.org/C99405784","wikidata":"https://www.wikidata.org/wiki/Q410434","display_name":"c-Raf","level":5,"score":0.41616880893707275},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4061850905418396},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3500405251979828},{"id":"https://openalex.org/C82495950","wikidata":"https://www.wikidata.org/wiki/Q14911732","display_name":"Cyclin-dependent kinase 2","level":4,"score":0.24071097373962402}],"mesh":[],"locations_count":3,"locations":[{"id":"pmh:oai:etheses.whiterose.ac.uk:8735","doi":null,"is_oa":false,"landing_page_url":"http://etheses.whiterose.ac.uk/8735/1/COMBINED_aftercorrections_FINAL.docx","pdf_url":null,"source":{"id":"https://openalex.org/S4306401585","display_name":"White Rose eTheses Online (University of Leeds, The University of Sheffield, University of York)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2800616092","host_organization_name":"White Rose University Consortium","host_organization_lineage":["https://openalex.org/I2800616092"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},{"id":"pmh:oai:uilis.unsyiah.ac.id:48733","doi":null,"is_oa":true,"landing_page_url":"http://etheses.whiterose.ac.uk/8735/1/COMBINED_aftercorrections_FINAL.docx","pdf_url":"http://uilis.unsyiah.ac.id/opentheses/items/show/48733","source":{"id":"https://openalex.org/S4377196378","display_name":"UPT. Syiah Kuala University Library (Syiah Kuala University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I187494767","host_organization_name":"Universitas Syiah Kuala","host_organization_lineage":["https://openalex.org/I187494767"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},{"id":"mag:2159168253","doi":null,"is_oa":false,"landing_page_url":"https://etheses.whiterose.ac.uk/8735/","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:uilis.unsyiah.ac.id:48733","doi":null,"is_oa":true,"landing_page_url":"http://etheses.whiterose.ac.uk/8735/1/COMBINED_aftercorrections_FINAL.docx","pdf_url":"http://uilis.unsyiah.ac.id/opentheses/items/show/48733","source":{"id":"https://openalex.org/S4377196378","display_name":"UPT. Syiah Kuala University Library (Syiah Kuala University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I187494767","host_organization_name":"Universitas Syiah Kuala","host_organization_lineage":["https://openalex.org/I187494767"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2159168253.pdf","grobid_xml":"https://content.openalex.org/works/W2159168253.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2086628330","https://openalex.org/W2076277651","https://openalex.org/W1995485025","https://openalex.org/W2559615161","https://openalex.org/W7097712224","https://openalex.org/W2913717237","https://openalex.org/W2052460480","https://openalex.org/W4413099876","https://openalex.org/W2031861066","https://openalex.org/W2753302145"],"abstract_inverted_index":{"Phosphoryl":[0],"transfer":[1,231],"is":[2,99,108,138,155,184,200],"one":[3,42],"of":[4,16,43,60,92,144,188,193,204,224,234,262,274,292],"the":[5,44,72,114,123,129,142,152,189,205,222,232,271],"most":[6],"common":[7],"biological":[8],"reactions,":[9],"and":[10,36,62,86,112,125,147,226,281],"enzymes":[11],"catalysing":[12],"this":[13,134,268],"are":[14,208],"capable":[15],"producing":[17],"huge":[18],"rate":[19],"enhancements":[20],"that":[21,122,151,197,245],"has":[22],"led":[23],"to":[24,41,101,158,176,178,230,240],"great":[25],"interest":[26],"in":[27,49,52,64,78,141,260],"their":[28],"catalytic":[29],"mechanism.":[30],"This":[31,96],"thesis":[32],"focuses":[33,215],"on":[34,104,166,216],"p38\u03b1":[35,56,282],"MEK6,":[37],"protein":[38,145,168,228],"kinases":[39,146,229,247],"belonging":[40],"MAP":[45],"kinase":[46,124,218,290],"signalling":[47],"pathways":[48],"eukaryotic":[50],"cells,":[51],"which":[53,70,207,255],"MEK6":[54,93,225,280],"activates":[55],"by":[57],"dual":[58,135,174,210],"phosphorylation":[59],"tyrosine":[61],"threonine":[63],"its":[65],"activation":[66,105,285],"loop.":[67],"Protein":[68],"phosphatases":[69,149],"dephosphorylate":[71],"same":[73,130],"residues":[74],"play":[75],"a":[76,88,201,252,275,288],"role":[77],"regulating":[79],"these":[80,119,194,246],"pathways.":[81],"\\n":[82],"\\nChapter":[83,162,213],"3":[84],"demonstrates":[85],"investigates":[87],"novel":[89],"phosphatase":[90,97,126,182,198],"activity":[91,98,137,183,199,291],"towards":[94],"p38\u03b1.":[95],"found":[100],"be":[102,257],"dependent":[103,111],"loop":[106],"phosphorylation,":[107],"metal":[109],"cation":[110],"requires":[113],"general":[115],"base":[116],"aspartate.":[117],"Together":[118],"results":[120,192],"indicate":[121],"activities":[127],"utilise":[128],"active":[131,153],"site.":[132],"Whilst":[133],"kinase/phosphatase":[136],"unexpected,":[139],"similarities":[140],"mechanism":[143],"PPP/PPM":[148],"suggest":[150,196],"site":[154],"naturally":[156],"equipped":[157],"catalyse":[159],"both":[160,284],"reactions.":[161],"4":[163],"concerns":[164],"studies":[165,195],"other":[167,227],"kinases,":[169],"either":[170],"serine/threonine":[171],"specific":[172,202],"or":[173],"specificity,":[175],"try":[177],"determine":[179],"how":[180],"widely":[181],"preserved":[185],"amongst":[186],"members":[187],"kinome.":[190],"The":[191,242],"feature":[203],"MAP2K\u2019s":[206],"archetypal":[209],"specificity":[211],"kinases.":[212],"5":[214],"unexpected":[217,272],"activity,":[219],"firstly":[220],"investigating":[221],"ability":[223],"\u03b2-phosphate":[233],"ADP":[235,250],"(rather":[236],"than":[237],"ATP":[238,264],"\u03b3-phosphate)":[239],"substrate.":[241],"findings":[243],"show":[244],"can":[248],"use":[249],"as":[251],"phosphoryl":[253],"donor,":[254],"may":[256],"functionally":[258],"significant":[259],"conditions":[261],"limited":[263],"supply.":[265],"In":[266],"addition":[267],"chapter":[269],"describes":[270],"formation":[273],"transition-state":[276],"analog":[277],"complex":[278],"between":[279],"lacking":[283],"sites,":[286],"implying":[287],"nonspecific":[289],"MEK6.":[293]},"counts_by_year":[],"updated_date":"2026-09-09T07:26:56.047234","created_date":"2016-06-24T00:00:00"}