{"id":"https://openalex.org/W2159109012","doi":"https://doi.org/10.1099/00221287-131-8-2075","title":"Purification and Characterization of Flagella from Roseburia cecicola, an Obligately Anaerobic Bacterium","display_name":"Purification and Characterization of Flagella from Roseburia cecicola, an Obligately Anaerobic Bacterium","publication_year":1985,"publication_date":"1985-08-01","ids":{"openalex":"https://openalex.org/W2159109012","doi":"https://doi.org/10.1099/00221287-131-8-2075","mag":"2159109012","pmid":"https://pubmed.ncbi.nlm.nih.gov/4056743"},"language":"en","primary_location":{"id":"doi:10.1099/00221287-131-8-2075","doi":"https://doi.org/10.1099/00221287-131-8-2075","is_oa":false,"landing_page_url":"https://doi.org/10.1099/00221287-131-8-2075","pdf_url":null,"source":{"id":"https://openalex.org/S196799759","display_name":"Microbiology","issn_l":"1350-0872","issn":["1350-0872","1465-2080"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320497","host_organization_name":"Microbiology Society","host_organization_lineage":["https://openalex.org/P4310320497"],"host_organization_lineage_names":["Microbiology Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microbiology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"J. H. MARTIN","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I157725225","display_name":"University of Illinois Urbana-Champaign","ror":"https://ror.org/047426m28","country_code":"US","type":"education","lineage":["https://openalex.org/I157725225"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"J. H. MARTIN","raw_affiliation_strings":["Department of Microbiology, University of Illinois, 407 South Goodwin Avenue, Urbana, Illinois 61801, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, University of Illinois, 407 South Goodwin Avenue, Urbana, Illinois 61801, USA","institution_ids":["https://openalex.org/I157725225"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5110798906","display_name":"Dwayne C. Savage","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I157725225","display_name":"University of Illinois Urbana-Champaign","ror":"https://ror.org/047426m28","country_code":"US","type":"education","lineage":["https://openalex.org/I157725225"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"D. C. SAVAGE","raw_affiliation_strings":["Department of Microbiology, University of Illinois, 407 South Goodwin Avenue, Urbana, Illinois 61801, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, University of Illinois, 407 South Goodwin Avenue, Urbana, Illinois 61801, USA","institution_ids":["https://openalex.org/I157725225"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I157725225"],"apc_list":null,"apc_paid":null,"fwci":0.7517,"has_fulltext":false,"cited_by_count":12,"citation_normalized_percentile":{"value":0.73584634,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":94,"max":96},"biblio":{"volume":"131","issue":"8","first_page":"2075","last_page":"2078"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.9175000190734863,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.9175000190734863,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10943","display_name":"Escherichia coli research studies","score":0.021199999377131462,"subfield":{"id":"https://openalex.org/subfields/1310","display_name":"Endocrinology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13326","display_name":"Biochemical and Structural Characterization","score":0.018200000748038292,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/bacterial-flagella","display_name":"bacterial flagellum","score":0.8420000076293945},{"id":"https://openalex.org/keywords/flagellin","display_name":"flagellin","score":0.968999981880188},{"id":"https://openalex.org/keywords/obligate-anaerobes","display_name":"obligate anaerobes","score":0.9490000009536743},{"id":"https://openalex.org/keywords/caecal-mucosa","display_name":"caecal mucosa","score":0.6140000224113464},{"id":"https://openalex.org/keywords/amino-acid-composition","display_name":"amino acid composition","score":0.8040000200271606}],"concepts":[{"id":"https://openalex.org/C115811362","wikidata":"https://www.wikidata.org/wiki/Q189998","display_name":"Flagellum","level":3,"score":0.8220410346984863},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.6412652730941772},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.62852942943573},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.530953586101532},{"id":"https://openalex.org/C2992397963","wikidata":"https://www.wikidata.org/wiki/Q189790","display_name":"Anaerobic bacteria","level":3,"score":0.45905837416648865},{"id":"https://openalex.org/C5140985","wikidata":"https://www.wikidata.org/wiki/Q483866","display_name":"Anaerobic exercise","level":2,"score":0.44847556948661804},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.06710442900657654},{"id":"https://openalex.org/C42407357","wikidata":"https://www.wikidata.org/wiki/Q521","display_name":"Physiology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000596","descriptor_name":"Amino Acids","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D001421","descriptor_name":"Bacteria, Anaerobic","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D004591","descriptor_name":"Electrophoresis, Polyacrylamide Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005407","descriptor_name":"Flagella","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D005408","descriptor_name":"Flagellin","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1099/00221287-131-8-2075","doi":"https://doi.org/10.1099/00221287-131-8-2075","is_oa":false,"landing_page_url":"https://doi.org/10.1099/00221287-131-8-2075","pdf_url":null,"source":{"id":"https://openalex.org/S196799759","display_name":"Microbiology","issn_l":"1350-0872","issn":["1350-0872","1465-2080"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320497","host_organization_name":"Microbiology Society","host_organization_lineage":["https://openalex.org/P4310320497"],"host_organization_lineage_names":["Microbiology Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microbiology","raw_type":"journal-article"},{"id":"pmid:4056743","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/4056743","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of general microbiology","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G221420316","display_name":null,"funder_award_id":"AI-11858","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"}],"funders":[{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":14,"referenced_works":["https://openalex.org/W1588063067","https://openalex.org/W1965028224","https://openalex.org/W2001193614","https://openalex.org/W2048822695","https://openalex.org/W2062949522","https://openalex.org/W2067405295","https://openalex.org/W2073879684","https://openalex.org/W2085430716","https://openalex.org/W2100837269","https://openalex.org/W2115539438","https://openalex.org/W2119703932","https://openalex.org/W2150021121","https://openalex.org/W2174880155","https://openalex.org/W2411923715"],"related_works":["https://openalex.org/W1564737620","https://openalex.org/W2591157153","https://openalex.org/W2119703932","https://openalex.org/W2047249174","https://openalex.org/W1520562824","https://openalex.org/W2562393936","https://openalex.org/W2167849015","https://openalex.org/W2097838115","https://openalex.org/W1586432184","https://openalex.org/W2129424847"],"abstract_inverted_index":{"Flagella":[0],"from":[1,10],"Roseburia":[2],"cecicola,":[3],"an":[4,50],"obligately":[5],"anaerobic":[6,71],"bacterium":[7],"originally":[8],"isolated":[9],"murine":[11],"caecal":[12],"mucosa,":[13],"were":[14,41],"purified":[15],"by":[16,20,30],"mechanical":[17],"shearing":[18],"followed":[19],"differential":[21],"centrifugation.":[22],"Purity":[23],"of":[24,43,54,61,68],"the":[25,62],"flagellar":[26],"preparation":[27],"was":[28,64],"determined":[29],"polyacrylamide":[31],"gel":[32],"electrophoresis,":[33],"electron":[34],"microscopy":[35],"and":[36,72],"chemical":[37],"analysis.":[38],"The":[39,57],"flagella":[40],"composed":[42],"a":[44],"single":[45],"protein":[46],"subunit":[47],"(flagellin)":[48],"with":[49],"estimated":[51],"molecular":[52],"weight":[53],"42":[55],"000.":[56],"amino":[58],"acid":[59],"composition":[60],"flagellin":[63],"similar":[65],"to":[66],"that":[67],"some":[69],"facultatively":[70],"aerobic":[73],"bacteria.":[74]},"counts_by_year":[{"year":2013,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}