{"id":"https://openalex.org/W215908684","doi":null,"title":"Ligand specificity and conformational states of the acetylcholine binding protein, a nicotinic receptor surrogate","display_name":"Ligand specificity and conformational states of the acetylcholine binding protein, a nicotinic receptor surrogate","publication_year":2005,"publication_date":"2005-01-01","ids":{"openalex":"https://openalex.org/W215908684","mag":"215908684"},"language":"en","primary_location":{"id":"pmh:oai:escholarship.org/ark:/13030/qt49p5m05v","doi":null,"is_oa":true,"landing_page_url":"https://escholarship.org/uc/item/49p5m05v","pdf_url":"https://escholarship.org/uc/item/49p5m05v","source":{"id":"https://openalex.org/S4306400115","display_name":"eScholarship (California Digital Library)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2801248553","host_organization_name":"California Digital Library","host_organization_lineage":["https://openalex.org/I2801248553"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"etd"},"volume":null,"type":"dissertation","indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://escholarship.org/uc/item/49p5m05v","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5019457716","display_name":"Scott B. Hansen","orcid":"https://orcid.org/0000-0003-0086-9753","observed_orcids":["https://orcid.org/0000-0003-0086-9753"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Scott B. Hansen","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0003-0086-9753","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5019457716"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11818","display_name":"Nicotinic Acetylcholine Receptors Study","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11818","display_name":"Nicotinic Acetylcholine Receptors Study","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11178","display_name":"Receptor Mechanisms and Signaling","score":0.0006000000284984708,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/acetylcholine-binding-protein","display_name":"acetylcholine binding protein","score":0.9980000257492065},{"id":"https://openalex.org/keywords/nicotinic-acetylcholine-receptors","display_name":"nicotinic acetylcholine receptors","score":0.9739999771118164},{"id":"https://openalex.org/keywords/ligand-binding-domain","display_name":"ligand-binding domain","score":0.9100000262260437},{"id":"https://openalex.org/keywords/ligand-specificity","display_name":"ligand specificity","score":0.8880000114440918},{"id":"https://openalex.org/keywords/conformational-change","display_name":"conformational change","score":0.8489999771118164},{"id":"https://openalex.org/keywords/loop-c","display_name":"loop C","score":0.753000020980835},{"id":"https://openalex.org/keywords/alpha-cobratoxin","display_name":"alpha-cobratoxin","score":0.9039999842643738},{"id":"https://openalex.org/keywords/methyllycaconitine","display_name":"methyllycaconitine","score":0.9959999918937683},{"id":"https://openalex.org/keywords/epibatidine","display_name":"epibatidine","score":0.9639999866485596},{"id":"https://openalex.org/keywords/lobeline","display_name":"lobeline","score":0.9919999837875366},{"id":"https://openalex.org/keywords/tryptophan-fluorescence","display_name":"tryptophan fluorescence","score":0.7490000128746033},{"id":"https://openalex.org/keywords/binding-kinetics","display_name":"binding kinetics","score":0.5080000162124634},{"id":"https://openalex.org/keywords/equilibrium-dissociation-constant","display_name":"equilibrium dissociation constant","score":0.7020000219345093},{"id":"https://openalex.org/keywords/crystal-structure","display_name":"crystal structure","score":0.46399998664855957},{"id":"https://openalex.org/keywords/lymnaea","display_name":"Lymnaea","score":0.6690000295639038},{"id":"https://openalex.org/keywords/aplysia","display_name":"Aplysia","score":1.0}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6994304656982422},{"id":"https://openalex.org/C188987157","wikidata":"https://www.wikidata.org/wiki/Q7030581","display_name":"Nicotinic agonist","level":3,"score":0.6398698687553406},{"id":"https://openalex.org/C2779570518","wikidata":"https://www.wikidata.org/wiki/Q412256","display_name":"Nicotinic acetylcholine receptor","level":4,"score":0.5884034633636475},{"id":"https://openalex.org/C2780374058","wikidata":"https://www.wikidata.org/wiki/Q3333710","display_name":"Methyllycaconitine","level":5,"score":0.5210856199264526},{"id":"https://openalex.org/C80161118","wikidata":"https://www.wikidata.org/wiki/Q408520","display_name":"Acetylcholine receptor","level":3,"score":0.5072371959686279},{"id":"https://openalex.org/C116569031","wikidata":"https://www.wikidata.org/wiki/Q899107","display_name":"Ligand (biochemistry)","level":3,"score":0.5009164810180664},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.4810678958892822},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.4653414487838745},{"id":"https://openalex.org/C82617044","wikidata":"https://www.wikidata.org/wiki/Q418536","display_name":"Ligand-gated ion channel","level":4,"score":0.44768643379211426},{"id":"https://openalex.org/C2775910092","wikidata":"https://www.wikidata.org/wiki/Q180623","display_name":"Acetylcholine","level":2,"score":0.43079429864883423},{"id":"https://openalex.org/C107824862","wikidata":"https://www.wikidata.org/wiki/Q616005","display_name":"Binding site","level":2,"score":0.41993600130081177},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3544127941131592},{"id":"https://openalex.org/C50254741","wikidata":"https://www.wikidata.org/wiki/Q62536","display_name":"Ion channel","level":3,"score":0.30018651485443115},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.26549646258354187},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.16133105754852295},{"id":"https://openalex.org/C98274493","wikidata":"https://www.wikidata.org/wiki/Q128406","display_name":"Pharmacology","level":1,"score":0.07679912447929382}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:escholarship.org/ark:/13030/qt49p5m05v","doi":null,"is_oa":true,"landing_page_url":"https://escholarship.org/uc/item/49p5m05v","pdf_url":"https://escholarship.org/uc/item/49p5m05v","source":{"id":"https://openalex.org/S4306400115","display_name":"eScholarship (California Digital Library)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2801248553","host_organization_name":"California Digital Library","host_organization_lineage":["https://openalex.org/I2801248553"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"etd"},{"id":"mag:215908684","doi":null,"is_oa":false,"landing_page_url":"https://escholarship.org/content/qt49p5m05v/qt49p5m05v.pdf?t=lq6h47","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:escholarship.org/ark:/13030/qt49p5m05v","doi":null,"is_oa":true,"landing_page_url":"https://escholarship.org/uc/item/49p5m05v","pdf_url":"https://escholarship.org/uc/item/49p5m05v","source":{"id":"https://openalex.org/S4306400115","display_name":"eScholarship (California Digital Library)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2801248553","host_organization_name":"California Digital Library","host_organization_lineage":["https://openalex.org/I2801248553"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"etd"},"sustainable_development_goals":[],"sustainable_development_goals_aurora":[{"score":0.4399999976158142,"display_name":"Life below water","id":"https://metadata.un.org/sdg/14"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G1824993827","display_name":null,"funder_award_id":"R37GM18360","funder_id":"https://openalex.org/F4320332505","funder_display_name":"U.S. Public Health Service"},{"id":"https://openalex.org/G741221119","display_name":null,"funder_award_id":"GM18360","funder_id":"https://openalex.org/F4320332505","funder_display_name":"U.S. Public Health Service"}],"funders":[{"id":"https://openalex.org/F4320320887","display_name":"European Synchrotron Radiation Facility","ror":"https://ror.org/02550n020"},{"id":"https://openalex.org/F4320322892","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73"},{"id":"https://openalex.org/F4320332505","display_name":"U.S. Public Health Service","ror":"https://ror.org/05xf94514"},{"id":"https://openalex.org/F4320332603","display_name":"University of California, San Diego","ror":"https://ror.org/0168r3w48"},{"id":"https://openalex.org/F4320338284","display_name":"Argonne National Laboratory","ror":"https://ror.org/05gvnxz63"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W215908684.pdf","grobid_xml":"https://content.openalex.org/works/W215908684.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1972959673","https://openalex.org/W2097345469","https://openalex.org/W1971716578","https://openalex.org/W2154197432","https://openalex.org/W2009828274","https://openalex.org/W2072134026","https://openalex.org/W237797323","https://openalex.org/W7098637131","https://openalex.org/W2133552860","https://openalex.org/W2043163467"],"abstract_inverted_index":{"The":[0,29,45,289],"acetylcholine":[1,14],"binding":[2,18,119,214,238,245,300,325],"protein":[3,31],"(AChBP)":[4],"is":[5,22,32,49],"a":[6,23,41,100,127,171,276,293],"structural":[7,68,322],"and":[8,54,57,67,77,123,145,149,201,211,223,287,328,337],"functional":[9],"surrogate":[10],"of":[11,26,38,47,70,115,120,143,166,187,219,252,270,279,285,321,340],"the":[12,27,35,89,109,153,164,220,235,249,253,256,259,263,299,308,338],"nicotinic":[13],"receptor":[15],"(nAChR)":[16],"ligand":[17,63,71],"domain":[19],"(LBD).":[20],"AChBP":[21,48,79,177,271,282],"natural":[24],"truncation":[25],"nAChR.":[28],"soluble":[30],"secreted":[33],"into":[34],"cholinergic":[36],"synapse":[37],"mollusks":[39],"as":[40],"primitive":[42],"synaptic":[43],"modulator.":[44],"solubility":[46],"amenable":[50],"to":[51,61,183,204,217,297,313,318],"fluorescence":[52],"spectroscopy":[53],"X-ray":[55],"crystallography":[56],"provides":[58],"an":[59],"opportunity":[60],"investigate":[62],"related":[64],"conformational":[65,268,295,330],"changes":[66,331],"determinants":[69,323],"binding.":[72],"Material":[73],"for":[74,88,113,147,152,159,191,199,324],"studying":[75],"Lymnaea":[76],"Aplysia":[78,179,281],"was":[80,95,111,181,215],"obtained":[81],"by":[82,136,274],"chemically":[83],"synthesizing":[84],"genes":[85],"(cDNA)":[86],"encoding":[87],"proteins.":[90,117],"A":[91,104],"mammalian":[92],"expression":[93,114],"system":[94],"devised":[96],"in":[97,126,130,170,267],"combination":[98],"with":[99,161,230,283],"FLAG-antibody":[101],"immunopurification":[102],"protocol.":[103],"single":[105],"FLAG":[106],"tag":[107],"on":[108,248,258],"N-terminus":[110],"optimal":[112],"both":[116],"Ligand":[118],"most":[121],"agonists":[122,148,200,221,303],"antagonists":[124,202,286],"resulted":[125],"substantial":[128],"change":[129,296],"intrinsic":[131],"tryptophan":[132],"fluorescence.":[133],"Kinetic":[134],"measurements":[135],"stopped-":[137],"flow":[138],"techniques":[139],"showed":[140],"rapid":[141],"rates":[142,151,162],"association":[144],"dissociation":[146,157],"slow":[150],"a-":[154,192],"neurotoxins.":[155],"Equilibrium":[156],"constants":[158],"ligands":[160,233,327],"beyond":[163],"detection":[165],"stopped-flow":[167],"were":[168,197,228,272],"measured":[169],"fluorescent":[172],"plate":[173],"reader":[174],"at":[175],"equilibrium.":[176],"from":[178],"californica":[180],"shown":[182],"have":[184],"five":[185],"orders":[186],"magnitude":[188],"higher":[189],"affinity":[190],"conotoxin":[193],"ImI.":[194],"Crystal":[195],"structures":[196],"determined":[198],"bound":[203,232],"AChBP.":[205],"Antagonists":[206,240,306],"included":[207],"a-conotoxin":[208],"ImI,":[209],"a-cobratoxin,":[210],"methyllycaconitine.":[212],"Antagonist":[213],"compared":[216,317],"that":[218],"epibatidine":[222],"lobeline.":[224],"Ten":[225],"side":[226,251,261],"chains":[227],"associated":[229,329],"all":[231],"delineating":[234],"competitive":[236],"hydrophobic":[237],"pocket.":[239],"occupied":[241],"two":[242],"additional":[243],"non-overlapping":[244],"surfaces;":[246],"one":[247],"apical":[250],"C":[254,264,290,309],"loop":[255,291,310],"another":[257],"'membrane'":[260],"under":[262],"loop.":[265],"Changes":[266],"states":[269],"investigated":[273],"comparing":[275],"crystal":[277],"structure":[278],"apo":[280],"those":[284],"agonists.":[288,319],"undergoes":[292],"large":[294],"cap":[298],"pocket":[301],"when":[302,316],"are":[304],"bound.":[305],"extend":[307],"radially":[311],"up":[312],"11":[314],"\u00c5":[315],"Knowledge":[320],"selective":[326],"should":[332],"facilitate":[333],"rational":[334],"drug":[335],"design":[336],"understanding":[339],"nAChR":[341],"function":[342]},"counts_by_year":[],"updated_date":"2026-08-31T07:19:18.363097","created_date":"2016-06-24T00:00:00"}