{"id":"https://openalex.org/W2158284989","doi":"https://doi.org/10.1021/jp045727e","title":"Determination of Anisotropic Optical Constants and Surface Coverage of Molecular Films Using Polarized Visible ATR Spectroscopy. Application to Adsorbed Cytochrome c Films","display_name":"Determination of Anisotropic Optical Constants and Surface Coverage of Molecular Films Using Polarized Visible ATR Spectroscopy. Application to Adsorbed Cytochrome c Films","publication_year":2004,"publication_date":"2004-12-07","ids":{"openalex":"https://openalex.org/W2158284989","doi":"https://doi.org/10.1021/jp045727e","mag":"2158284989","pmid":"https://pubmed.ncbi.nlm.nih.gov/16851032"},"language":"en","primary_location":{"id":"doi:10.1021/jp045727e","doi":"https://doi.org/10.1021/jp045727e","is_oa":false,"landing_page_url":"https://doi.org/10.1021/jp045727e","pdf_url":null,"source":{"id":"https://openalex.org/S185621672","display_name":"The Journal of Physical Chemistry B","issn_l":"1520-5207","issn":["1520-5207","1520-6106"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of Physical Chemistry B","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5041647999","display_name":"Anne F. Runge","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I138006243","display_name":"University of Arizona","ror":"https://ror.org/03m2x1q45","country_code":"US","type":"education","lineage":["https://openalex.org/I138006243"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Anne F. Runge","raw_affiliation_strings":["Department of Chemistry, and Optical Sciences Center, University of Arizona, Tucson, Arizona 85721"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, and Optical Sciences Center, University of Arizona, Tucson, Arizona 85721","institution_ids":["https://openalex.org/I138006243"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5023848490","display_name":"Nicole C. Rasmussen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I138006243","display_name":"University of Arizona","ror":"https://ror.org/03m2x1q45","country_code":"US","type":"education","lineage":["https://openalex.org/I138006243"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Nicole C. Rasmussen","raw_affiliation_strings":["Department of Chemistry, and Optical Sciences Center, University of Arizona, Tucson, Arizona 85721"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, and Optical Sciences Center, University of Arizona, Tucson, Arizona 85721","institution_ids":["https://openalex.org/I138006243"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5006802932","display_name":"S. Scott Saavedra","orcid":"https://orcid.org/0000-0002-9946-2664","observed_orcids":["https://orcid.org/0000-0002-9946-2664"]},"institutions":[{"id":"https://openalex.org/I138006243","display_name":"University of Arizona","ror":"https://ror.org/03m2x1q45","country_code":"US","type":"education","lineage":["https://openalex.org/I138006243"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"S. Scott Saavedra","raw_affiliation_strings":["Department of Chemistry, and Optical Sciences Center, University of Arizona, Tucson, Arizona 85721"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, and Optical Sciences Center, University of Arizona, Tucson, Arizona 85721","institution_ids":["https://openalex.org/I138006243"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5054899604","display_name":"Sergio B. Mendes","orcid":"https://orcid.org/0000-0002-9462-9058","observed_orcids":["https://orcid.org/0000-0002-9462-9058"]},"institutions":[{"id":"https://openalex.org/I138006243","display_name":"University of Arizona","ror":"https://ror.org/03m2x1q45","country_code":"US","type":"education","lineage":["https://openalex.org/I138006243"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Sergio B. Mendes","raw_affiliation_strings":["Department of Chemistry, and Optical Sciences Center, University of Arizona, Tucson, Arizona 85721"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, and Optical Sciences Center, University of Arizona, Tucson, Arizona 85721","institution_ids":["https://openalex.org/I138006243"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I138006243"],"apc_list":null,"apc_paid":null,"fwci":1.718,"has_fulltext":false,"cited_by_count":26,"citation_normalized_percentile":{"value":0.84471045,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"109","issue":"1","first_page":"424","last_page":"431"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11324","display_name":"Spectroscopy Techniques in Biomedical and Chemical Research","score":0.6654999852180481,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11324","display_name":"Spectroscopy Techniques in Biomedical and Chemical Research","score":0.6654999852180481,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12050","display_name":"Optical Polarization and Ellipsometry","score":0.15690000355243683,"subfield":{"id":"https://openalex.org/subfields/3107","display_name":"Atomic and Molecular Physics, and Optics"},"field":{"id":"https://openalex.org/fields/31","display_name":"Physics and Astronomy"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10859","display_name":"Polymer Surface Interaction Studies","score":0.02630000002682209,"subfield":{"id":"https://openalex.org/subfields/2508","display_name":"Surfaces, Coatings and Films"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/surface-coverage","display_name":"surface coverage","score":0.7210000157356262},{"id":"https://openalex.org/keywords/molecular-films","display_name":"molecular films","score":0.6140000224113464},{"id":"https://openalex.org/keywords/transfer-matrix-formalism","display_name":"transfer matrix formalism","score":0.8820000290870667},{"id":"https://openalex.org/keywords/birefringent-films","display_name":"birefringent films","score":0.9020000100135803},{"id":"https://openalex.org/keywords/complex-refractive-index","display_name":"complex refractive index","score":0.9779999852180481},{"id":"https://openalex.org/keywords/adsorbed-protein-films","display_name":"adsorbed protein films","score":0.7379999756813049},{"id":"https://openalex.org/keywords/cytochrome-c","display_name":"cytochrome c","score":0.9959999918937683},{"id":"https://openalex.org/keywords/order-parameter","display_name":"order parameter","score":0.6830000281333923},{"id":"https://openalex.org/keywords/indium-tin-oxide","display_name":"indium tin oxide","score":0.7990000247955322},{"id":"https://openalex.org/keywords/glass-substrates","display_name":"glass substrates","score":0.7379999756813049}],"concepts":[{"id":"https://openalex.org/C32891209","wikidata":"https://www.wikidata.org/wiki/Q483666","display_name":"Spectroscopy","level":2,"score":0.6819441914558411},{"id":"https://openalex.org/C85725439","wikidata":"https://www.wikidata.org/wiki/Q466686","display_name":"Anisotropy","level":2,"score":0.6808559894561768},{"id":"https://openalex.org/C150394285","wikidata":"https://www.wikidata.org/wiki/Q180254","display_name":"Adsorption","level":2,"score":0.6122931241989136},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.5276461839675903},{"id":"https://openalex.org/C2776799497","wikidata":"https://www.wikidata.org/wiki/Q484298","display_name":"Surface (topology)","level":2,"score":0.4412088394165039},{"id":"https://openalex.org/C113196181","wikidata":"https://www.wikidata.org/wiki/Q485223","display_name":"Analytical Chemistry (journal)","level":2,"score":0.4081836938858032},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.30641478300094604},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.3057880401611328},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.17249327898025513},{"id":"https://openalex.org/C147789679","wikidata":"https://www.wikidata.org/wiki/Q11372","display_name":"Physical chemistry","level":1,"score":0.16427239775657654},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.08097237348556519},{"id":"https://openalex.org/C2524010","wikidata":"https://www.wikidata.org/wiki/Q8087","display_name":"Geometry","level":1,"score":0.0},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.0},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000327","descriptor_name":"Adsorption","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006736","descriptor_name":"Horses","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008567","descriptor_name":"Membranes, Artificial","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012680","descriptor_name":"Sensitivity and Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013057","descriptor_name":"Spectrum Analysis","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D013499","descriptor_name":"Surface Properties","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016880","descriptor_name":"Anisotropy","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D045304","descriptor_name":"Cytochromes c","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1021/jp045727e","doi":"https://doi.org/10.1021/jp045727e","is_oa":false,"landing_page_url":"https://doi.org/10.1021/jp045727e","pdf_url":null,"source":{"id":"https://openalex.org/S185621672","display_name":"The Journal of Physical Chemistry B","issn_l":"1520-5207","issn":["1520-5207","1520-6106"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of Physical Chemistry B","raw_type":"journal-article"},{"id":"pmid:16851032","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/16851032","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The journal of physical chemistry. B","raw_type":"Journal Article"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.486.6982","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.486.6982","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://pyramid.spd.louisville.edu/~eri/papers_pres/Mendes_JPC_2005.pdf","raw_type":"text"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":43,"referenced_works":["https://openalex.org/W1569367682","https://openalex.org/W1976133345","https://openalex.org/W1976595819","https://openalex.org/W1978602946","https://openalex.org/W1981599160","https://openalex.org/W1985291256","https://openalex.org/W1987113590","https://openalex.org/W1987265289","https://openalex.org/W1991485581","https://openalex.org/W2002427776","https://openalex.org/W2013291571","https://openalex.org/W2014904816","https://openalex.org/W2016982893","https://openalex.org/W2019992819","https://openalex.org/W2021432737","https://openalex.org/W2021616514","https://openalex.org/W2029563574","https://openalex.org/W2036316665","https://openalex.org/W2040882982","https://openalex.org/W2047869488","https://openalex.org/W2054662671","https://openalex.org/W2056979165","https://openalex.org/W2060424667","https://openalex.org/W2068193830","https://openalex.org/W2069706056","https://openalex.org/W2070771977","https://openalex.org/W2079612824","https://openalex.org/W2079990979","https://openalex.org/W2082120663","https://openalex.org/W2082832628","https://openalex.org/W2088803443","https://openalex.org/W2103145586","https://openalex.org/W2107791741","https://openalex.org/W2122552600","https://openalex.org/W2130313059","https://openalex.org/W2131561087","https://openalex.org/W2133439000","https://openalex.org/W2134665113","https://openalex.org/W2164280110","https://openalex.org/W2224792910","https://openalex.org/W2786228416","https://openalex.org/W3127048131","https://openalex.org/W4299908858"],"related_works":["https://openalex.org/W2033211088","https://openalex.org/W2499766163","https://openalex.org/W2019375590","https://openalex.org/W2072666919","https://openalex.org/W1976327599","https://openalex.org/W2066480817","https://openalex.org/W2014138515","https://openalex.org/W2060455928","https://openalex.org/W2012985221","https://openalex.org/W2044812553"],"abstract_inverted_index":{"This":[0],"article":[1],"describes":[2],"a":[3],"method":[4],"to":[5,31,49,113],"determine":[6,50],"the":[7,28,51,56,64,69,76,80,86,89,97],"anisotropic":[8,52],"optical":[9],"constants":[10],"and":[11,34,40,59,92,115,150,169],"surface":[12,90,142],"coverage":[13,91],"of":[14,54,63,68,75,79,88,96],"molecular":[15],"films":[16,36,111,127],"using":[17],"polarized":[18],"attenuated":[19],"total":[20],"reflectance":[21],"(ATR)":[22],"absorbance":[23],"measurements.":[24],"We":[25],"have":[26,41,104,137],"extended":[27],"transfer-matrix":[29],"formalism":[30],"describe":[32],"birefringent":[33],"dichroic":[35],"in":[37,140],"ATR":[38],"geometries":[39],"combined":[42],"it":[43],"with":[44],"an":[45],"iterative":[46],"numerical":[47],"procedure":[48],"values":[53,74],"both":[55],"real":[57],"(n)":[58],"imaginary":[60,77],"(k)":[61,83],"parts":[62],"complex":[65],"refractive":[66,81],"index":[67,82],"film":[70],"under":[71,134],"investigation.":[72],"Anisotropic":[73],"part":[78],"allow":[84],"for":[85],"determination":[87],"one":[93],"order":[94,159],"parameter":[95],"film.":[98],"To":[99],"illustrate":[100],"this":[101],"approach,":[102],"we":[103],"used":[105],"cytochrome":[106],"c":[107,126],"(cyt":[108],"c)":[109],"protein":[110],"adsorbed":[112],"glass":[114,149,168],"indium":[116],"tin":[117],"oxide":[118],"(ITO)":[119],"surfaces.":[120],"Experimental":[121],"results":[122],"show":[123],"that":[124],"cyt":[125],"on":[128,148,155,167,173],"these":[129],"surfaces,":[130],"which":[131],"were":[132],"formed":[133],"identical":[135],"conditions,":[136],"significant":[138],"differences":[139],"their":[141,158],"coverages":[143],"(11.2":[144],"+/-":[145,152,165,171],"0.4":[146],"pmol/cm(2)":[147,154],"21.7":[151],"0.9":[153],"ITO);":[156],"however,":[157],"parameters":[160],"<cos(2)theta>":[161],"are":[162],"similar":[163],"(0.30":[164],"0.02":[166],"0.36":[170],"0.04":[172],"ITO).":[174]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2013,"cited_by_count":3},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}