{"id":"https://openalex.org/W2157756782","doi":"https://doi.org/10.1093/protein/gzn031","title":"Structural principles of the broad substrate specificity of Thermoactinomyces vulgaris carboxypeptidase T--role of amino acid residues at positions 260 and 262","display_name":"Structural principles of the broad substrate specificity of Thermoactinomyces vulgaris carboxypeptidase T--role of amino acid residues at positions 260 and 262","publication_year":2008,"publication_date":"2008-05-23","ids":{"openalex":"https://openalex.org/W2157756782","doi":"https://doi.org/10.1093/protein/gzn031","mag":"2157756782","pmid":"https://pubmed.ncbi.nlm.nih.gov/18515300"},"language":"en","primary_location":{"id":"doi:10.1093/protein/gzn031","doi":"https://doi.org/10.1093/protein/gzn031","is_oa":false,"landing_page_url":"https://doi.org/10.1093/protein/gzn031","pdf_url":null,"source":{"id":"https://openalex.org/S44492648","display_name":"Protein Engineering Design and Selection","issn_l":"0269-2139","issn":["0269-2139","1460-213X","1741-0126","1741-0134"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Protein Engineering Design and Selection","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5075658120","display_name":"Andrey M. Grishin","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210163576","display_name":"Genetika","ror":"https://ror.org/05hr7qe62","country_code":"RU","type":"facility","lineage":["https://openalex.org/I4210163576"]}],"countries":["RU"],"is_corresponding":false,"raw_author_name":"A.M. Grishin","raw_affiliation_strings":["The V.M. Stepanov Laboratory of Protein Chemistry, Institute of Genetics and Selection of Industrial Microorganisms 1, 1-ii Dorozhnyi Proezd, 117545 Moscow, Russian Federation"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The V.M. Stepanov Laboratory of Protein Chemistry, Institute of Genetics and Selection of Industrial Microorganisms 1, 1-ii Dorozhnyi Proezd, 117545 Moscow, Russian Federation","institution_ids":["https://openalex.org/I4210163576"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5015021049","display_name":"Valery Kh. Akparov","orcid":"https://orcid.org/0000-0002-5821-5632","observed_orcids":["https://orcid.org/0000-0002-5821-5632"]},"institutions":[{"id":"https://openalex.org/I4210163576","display_name":"Genetika","ror":"https://ror.org/05hr7qe62","country_code":"RU","type":"facility","lineage":["https://openalex.org/I4210163576"]}],"countries":["RU"],"is_corresponding":true,"raw_author_name":"V.K. Akparov","raw_affiliation_strings":["The V.M. Stepanov Laboratory of Protein Chemistry, Institute of Genetics and Selection of Industrial Microorganisms 1, 1-ii Dorozhnyi Proezd, 117545 Moscow, Russian Federation"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The V.M. Stepanov Laboratory of Protein Chemistry, Institute of Genetics and Selection of Industrial Microorganisms 1, 1-ii Dorozhnyi Proezd, 117545 Moscow, Russian Federation","institution_ids":["https://openalex.org/I4210163576"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5110639758","display_name":"Galina G. Chestukhina","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210163576","display_name":"Genetika","ror":"https://ror.org/05hr7qe62","country_code":"RU","type":"facility","lineage":["https://openalex.org/I4210163576"]}],"countries":["RU"],"is_corresponding":false,"raw_author_name":"G.G. Chestukhina","raw_affiliation_strings":["The V.M. Stepanov Laboratory of Protein Chemistry, Institute of Genetics and Selection of Industrial Microorganisms 1, 1-ii Dorozhnyi Proezd, 117545 Moscow, Russian Federation"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The V.M. Stepanov Laboratory of Protein Chemistry, Institute of Genetics and Selection of Industrial Microorganisms 1, 1-ii Dorozhnyi Proezd, 117545 Moscow, Russian Federation","institution_ids":["https://openalex.org/I4210163576"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5015021049"],"corresponding_institution_ids":["https://openalex.org/I4210163576"],"apc_list":{"value":4656,"currency":"USD","value_usd":4656},"apc_paid":null,"fwci":0.8326,"has_fulltext":false,"cited_by_count":13,"citation_normalized_percentile":{"value":0.70223558,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":95},"biblio":{"volume":"21","issue":"9","first_page":"545","last_page":"551"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.3407999873161316,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.3407999873161316,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.24819999933242798,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12505","display_name":"Peptidase Inhibition and Analysis","score":0.20000000298023224,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/thermoactinomyces-vulgaris","display_name":"Thermoactinomyces vulgaris","score":0.8999999761581421},{"id":"https://openalex.org/keywords/substrate-specificity","display_name":"substrate specificity","score":0.906000018119812},{"id":"https://openalex.org/keywords/s1-site","display_name":"S1 site","score":0.8600000143051147},{"id":"https://openalex.org/keywords/site-directed-mutagenesis","display_name":"site-directed mutagenesis","score":0.8199999928474426},{"id":"https://openalex.org/keywords/charged-substrates","display_name":"charged substrates","score":0.6470000147819519},{"id":"https://openalex.org/keywords/c-terminal-residues","display_name":"C-terminal residues","score":0.671999990940094},{"id":"https://openalex.org/keywords/active-site-loop","display_name":"active site loop","score":0.6499999761581421},{"id":"https://openalex.org/keywords/enzyme-structure-function-relationship","display_name":"enzyme structure-function","score":0.40299999713897705}],"concepts":[{"id":"https://openalex.org/C41183919","wikidata":"https://www.wikidata.org/wiki/Q423026","display_name":"Active site","level":3,"score":0.7397633194923401},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7023319005966187},{"id":"https://openalex.org/C108477463","wikidata":"https://www.wikidata.org/wiki/Q426367","display_name":"Carboxypeptidase","level":3,"score":0.695048451423645},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.6520596742630005},{"id":"https://openalex.org/C2781338088","wikidata":"https://www.wikidata.org/wiki/Q903495","display_name":"Residue (chemistry)","level":2,"score":0.6035300493240356},{"id":"https://openalex.org/C2777289219","wikidata":"https://www.wikidata.org/wiki/Q7632154","display_name":"Substrate (aquarium)","level":2,"score":0.561385989189148},{"id":"https://openalex.org/C2994376932","wikidata":"https://www.wikidata.org/wiki/Q735188","display_name":"Amino acid residue","level":4,"score":0.5577173233032227},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.5384454727172852},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5369104743003845},{"id":"https://openalex.org/C16318435","wikidata":"https://www.wikidata.org/wiki/Q149299","display_name":"Mutagenesis","level":4,"score":0.5294641852378845},{"id":"https://openalex.org/C103408237","wikidata":"https://www.wikidata.org/wiki/Q2642668","display_name":"Site-directed mutagenesis","level":4,"score":0.4276520609855652},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.40643882751464844},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.21146532893180847},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.1459396481513977},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.12441617250442505},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000596","descriptor_name":"Amino Acids","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D000596","descriptor_name":"Amino Acids","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002268","descriptor_name":"Carboxypeptidases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002268","descriptor_name":"Carboxypeptidases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D008848","descriptor_name":"Micromonosporaceae","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D008958","descriptor_name":"Models, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D011487","descriptor_name":"Protein Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016297","descriptor_name":"Mutagenesis, Site-Directed","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D057927","descriptor_name":"Hydrophobic and Hydrophilic Interactions","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1093/protein/gzn031","doi":"https://doi.org/10.1093/protein/gzn031","is_oa":false,"landing_page_url":"https://doi.org/10.1093/protein/gzn031","pdf_url":null,"source":{"id":"https://openalex.org/S44492648","display_name":"Protein Engineering Design and Selection","issn_l":"0269-2139","issn":["0269-2139","1460-213X","1741-0126","1741-0134"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Protein Engineering Design and Selection","raw_type":"journal-article"},{"id":"pmid:18515300","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/18515300","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Protein engineering, design & selection : PEDS","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":38,"referenced_works":["https://openalex.org/W1481687671","https://openalex.org/W1493722440","https://openalex.org/W1550713058","https://openalex.org/W1567730256","https://openalex.org/W1593632419","https://openalex.org/W1966321597","https://openalex.org/W1968592972","https://openalex.org/W1970766351","https://openalex.org/W1978637267","https://openalex.org/W1985469117","https://openalex.org/W1991606841","https://openalex.org/W1999166397","https://openalex.org/W1999372769","https://openalex.org/W2004091546","https://openalex.org/W2006458168","https://openalex.org/W2015642465","https://openalex.org/W2018209674","https://openalex.org/W2029667189","https://openalex.org/W2043855562","https://openalex.org/W2051439273","https://openalex.org/W2060913542","https://openalex.org/W2062951769","https://openalex.org/W2070096162","https://openalex.org/W2071348438","https://openalex.org/W2091123783","https://openalex.org/W2097264902","https://openalex.org/W2100837269","https://openalex.org/W2104017463","https://openalex.org/W2107685496","https://openalex.org/W2127522551","https://openalex.org/W2128635872","https://openalex.org/W2144634347","https://openalex.org/W2152782018","https://openalex.org/W2161541337","https://openalex.org/W2171268876","https://openalex.org/W2408909170","https://openalex.org/W2412593163","https://openalex.org/W2412621928"],"related_works":["https://openalex.org/W1970766351","https://openalex.org/W1981294340","https://openalex.org/W1992093544","https://openalex.org/W2889665720","https://openalex.org/W3196645918","https://openalex.org/W2462855413","https://openalex.org/W2914787331","https://openalex.org/W2997084639","https://openalex.org/W3115084050","https://openalex.org/W4400986138"],"abstract_inverted_index":{"An":[0],"influence":[1],"of":[2,14,25,30,33,39,52,71,82,106,123,130,146,191,203],"residues":[3,195],"at":[4,196],"positions":[5,197],"260":[6,198],"and":[7,44,64,96,128,136,170,181,199],"262":[8,200],"on":[9],"a":[10,112,124,131],"broad":[11],"substrate":[12,206],"specificity":[13,145],"Thermoactinomyces":[15],"vulgaris":[16],"carboxypeptidase":[17],"T":[18],"(CPT)":[19],"has":[20],"been":[21],"studied":[22],"by":[23,79,92],"means":[24],"site-directed":[26],"mutagenesis.":[27],"The":[28,68,104,121,154,184],"structure":[29],"the":[31,48,72,86,101,140,144,147,188,192,204],"S1'-site":[32],"CPT":[34,78,137,164,168,173,205],"is":[35,50],"similar":[36],"to":[37,111],"those":[38],"pancreatic":[40],"carboxypeptidases":[41],"A":[42],"(CPA)":[43],"B":[45],"(CPB);":[46],"however,":[47],"enzyme":[49,102],"capable":[51],"cleaving":[53],"off":[54],"C-terminal":[55,59,152,178],"hydrophobic":[56,75],"(like":[57,62],"CPA),":[58],"positively":[60],"charged":[61,66,119],"CPB),":[63],"negatively":[65],"residues.":[67],"spatial":[69],"alteration":[70],"S1'":[73,141],"site":[74,88,142],"area":[76],"in":[77,85,116,139,201],"an":[80],"insertion":[81],"one":[83],"residue":[84],"active":[87],"loop":[89],"with":[90,94,151,177],"Tyr255":[91],"analogy":[93],"CPA":[95],"CPB":[97],"did":[98],"not":[99],"change":[100],"specificity.":[103,207],"introduction":[105],"Ile262":[107],"(CPT":[108,126,134],"D260G/T262I)":[109],"led":[110],"statistically":[113],"significant":[114],"reduction":[115],"activity":[117],"towards":[118,149],"substrates.":[120],"removal":[122],"negative":[125],"D260G)":[127],"placement":[129],"positive":[132],"charge":[133],"D260G/T262K":[135,169],"D260G/T262R)":[138],"shifted":[143],"variants":[148],"substrates":[150,176],"Glu.":[153],"selectivity":[155],"profile":[156],"was":[157],"64:1.7:1":[158],"for":[159,163,167,172,175],"wild-type":[160],"CPT,":[161],"815:115:1":[162],"D260G,":[165],"3270:1060:1":[166],"1:2.4:0":[171],"D260G/T262R":[174],"Leu,":[179],"Glu":[180],"Arg,":[182],"respectively.":[183],"obtained":[185],"results":[186],"confirm":[187],"important":[189],"role":[190],"amino":[193],"acid":[194],"determination":[202]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}