{"id":"https://openalex.org/W2157253100","doi":"https://doi.org/10.1242/jcs.106.4.1347","title":"Orientation of macromolecules in the walls of elongating carrot cells","display_name":"Orientation of macromolecules in the walls of elongating carrot cells","publication_year":1993,"publication_date":"1993-12-01","ids":{"openalex":"https://openalex.org/W2157253100","doi":"https://doi.org/10.1242/jcs.106.4.1347","mag":"2157253100","pmid":"https://pubmed.ncbi.nlm.nih.gov/7510302"},"language":"en","primary_location":{"id":"doi:10.1242/jcs.106.4.1347","doi":"https://doi.org/10.1242/jcs.106.4.1347","is_oa":false,"landing_page_url":"https://doi.org/10.1242/jcs.106.4.1347","pdf_url":null,"source":{"id":"https://openalex.org/S5677253","display_name":"Journal of Cell Science","issn_l":"0021-9533","issn":["0021-9533","1477-9137"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310311847","host_organization_name":"The Company of Biologists","host_organization_lineage":["https://openalex.org/P4310311847"],"host_organization_lineage_names":["The Company of Biologists"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Cell Science","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5052280720","display_name":"Maureen C. McCann","orcid":"https://orcid.org/0000-0001-6956-4216","observed_orcids":["https://orcid.org/0000-0001-6956-4216"]},"institutions":[{"id":"https://openalex.org/I100288624","display_name":"John Innes Centre","ror":"https://ror.org/055zmrh94","country_code":"GB","type":"facility","lineage":["https://openalex.org/I100288624","https://openalex.org/I2799693246","https://openalex.org/I4210087105"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"M. C. McCann","raw_affiliation_strings":["John Innes Institute 1 Department of Cell Biology , , Colney Lane, Norwich NR4 7UH, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"John Innes Institute 1 Department of Cell Biology , , Colney Lane, Norwich NR4 7UH, UK","institution_ids":["https://openalex.org/I100288624"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111622388","display_name":"N. J. Stacey","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I100288624","display_name":"John Innes Centre","ror":"https://ror.org/055zmrh94","country_code":"GB","type":"facility","lineage":["https://openalex.org/I100288624","https://openalex.org/I2799693246","https://openalex.org/I4210087105"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"N. J. Stacey","raw_affiliation_strings":["John Innes Institute 1 Department of Cell Biology , , Colney Lane, Norwich NR4 7UH, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"John Innes Institute 1 Department of Cell Biology , , Colney Lane, Norwich NR4 7UH, UK","institution_ids":["https://openalex.org/I100288624"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111670261","display_name":"Reginald Wilson","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I3018370718","display_name":"Quadram Institute","ror":"https://ror.org/04td3ys19","country_code":"GB","type":"facility","lineage":["https://openalex.org/I2799693246","https://openalex.org/I3018370718","https://openalex.org/I4210087105"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"R. Wilson","raw_affiliation_strings":["2 AFRC Institute of Food Research, Colney Lane, Norwich NR4 7UA, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"2 AFRC Institute of Food Research, Colney Lane, Norwich NR4 7UA, UK","institution_ids":["https://openalex.org/I3018370718"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5072138276","display_name":"Keith Roberts","orcid":"https://orcid.org/0000-0001-9148-6701","observed_orcids":["https://orcid.org/0000-0001-9148-6701"]},"institutions":[{"id":"https://openalex.org/I100288624","display_name":"John Innes Centre","ror":"https://ror.org/055zmrh94","country_code":"GB","type":"facility","lineage":["https://openalex.org/I100288624","https://openalex.org/I2799693246","https://openalex.org/I4210087105"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"K. Roberts","raw_affiliation_strings":["John Innes Institute 1 Department of Cell Biology , , Colney Lane, Norwich NR4 7UH, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"John Innes Institute 1 Department of Cell Biology , , Colney Lane, Norwich NR4 7UH, UK","institution_ids":["https://openalex.org/I100288624"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":6.4168,"has_fulltext":false,"cited_by_count":81,"citation_normalized_percentile":{"value":0.96620464,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":87,"max":97},"biblio":{"volume":"106","issue":"4","first_page":"1347","last_page":"1356"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10869","display_name":"Polysaccharides and Plant Cell Walls","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10869","display_name":"Polysaccharides and Plant Cell Walls","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10184","display_name":"Plant Molecular Biology Research","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12161","display_name":"Plant Surface Properties and Treatments","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cell-elongation","display_name":"cell elongation","score":0.8970000147819519},{"id":"https://openalex.org/keywords/carrot","display_name":"carrot","score":0.5509999990463257},{"id":"https://openalex.org/keywords/cellulose-microfibrils","display_name":"cellulose microfibrils","score":0.8349999785423279},{"id":"https://openalex.org/keywords/cell-wall-composition","display_name":"cell wall composition","score":0.6690000295639038},{"id":"https://openalex.org/keywords/pectin","display_name":"pectin","score":0.7670000195503235},{"id":"https://openalex.org/keywords/cell-wall-proteins","display_name":"cell wall proteins","score":0.8500000238418579},{"id":"https://openalex.org/keywords/esters","display_name":"esters","score":0.49799999594688416},{"id":"https://openalex.org/keywords/phenolic-compounds","display_name":"phenolic compounds","score":0.640999972820282},{"id":"https://openalex.org/keywords/cell-wall-architecture","display_name":"cell wall architecture","score":0.41600000858306885}],"concepts":[{"id":"https://openalex.org/C33947775","wikidata":"https://www.wikidata.org/wiki/Q271439","display_name":"Elongation","level":3,"score":0.8267205953598022},{"id":"https://openalex.org/C125235067","wikidata":"https://www.wikidata.org/wiki/Q128700","display_name":"Cell wall","level":2,"score":0.6486391425132751},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.5080974102020264},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.49023371934890747},{"id":"https://openalex.org/C2779251873","wikidata":"https://www.wikidata.org/wiki/Q80294","display_name":"Cellulose","level":2,"score":0.4690191149711609},{"id":"https://openalex.org/C100817775","wikidata":"https://www.wikidata.org/wiki/Q134219","display_name":"Polysaccharide","level":2,"score":0.44993215799331665},{"id":"https://openalex.org/C160892712","wikidata":"https://www.wikidata.org/wiki/Q901559","display_name":"Fourier transform infrared spectroscopy","level":2,"score":0.4385886490345001},{"id":"https://openalex.org/C8010536","wikidata":"https://www.wikidata.org/wiki/Q160398","display_name":"Crystallography","level":1,"score":0.4052931070327759},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.36673393845558167},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.18928125500679016},{"id":"https://openalex.org/C159985019","wikidata":"https://www.wikidata.org/wiki/Q181790","display_name":"Composite material","level":1,"score":0.17661583423614502},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.17415541410446167},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.14673897624015808},{"id":"https://openalex.org/C112950240","wikidata":"https://www.wikidata.org/wiki/Q76005","display_name":"Ultimate tensile strength","level":2,"score":0.12985938787460327},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D000911","descriptor_name":"Antibodies, Monoclonal","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000939","descriptor_name":"Epitopes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002473","descriptor_name":"Cell Wall","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":true},{"descriptor_ui":"D007150","descriptor_name":"Immunohistochemistry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014675","descriptor_name":"Vegetables","qualifier_ui":"Q000254","qualifier_name":"growth & development","is_major_topic":true},{"descriptor_ui":"D014675","descriptor_name":"Vegetables","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":true},{"descriptor_ui":"D016591","descriptor_name":"Histocytological Preparation Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017550","descriptor_name":"Spectroscopy, Fourier Transform Infrared","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1242/jcs.106.4.1347","doi":"https://doi.org/10.1242/jcs.106.4.1347","is_oa":false,"landing_page_url":"https://doi.org/10.1242/jcs.106.4.1347","pdf_url":null,"source":{"id":"https://openalex.org/S5677253","display_name":"Journal of Cell Science","issn_l":"0021-9533","issn":["0021-9533","1477-9137"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310311847","host_organization_name":"The Company of Biologists","host_organization_lineage":["https://openalex.org/P4310311847"],"host_organization_lineage_names":["The Company of Biologists"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Cell Science","raw_type":"journal-article"},{"id":"pmid:7510302","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7510302","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of cell science","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":26,"referenced_works":["https://openalex.org/W170289352","https://openalex.org/W980522815","https://openalex.org/W1450593323","https://openalex.org/W1501365185","https://openalex.org/W1768711802","https://openalex.org/W1877857937","https://openalex.org/W1889241553","https://openalex.org/W1973271025","https://openalex.org/W2001239076","https://openalex.org/W2003219510","https://openalex.org/W2010485711","https://openalex.org/W2024556021","https://openalex.org/W2043482474","https://openalex.org/W2057892597","https://openalex.org/W2066567158","https://openalex.org/W2073201617","https://openalex.org/W2073553052","https://openalex.org/W2073883142","https://openalex.org/W2129002076","https://openalex.org/W2139053374","https://openalex.org/W2152037193","https://openalex.org/W2173216418","https://openalex.org/W2313591930","https://openalex.org/W2344701987","https://openalex.org/W2493624714","https://openalex.org/W4240443566"],"related_works":["https://openalex.org/W1980238507","https://openalex.org/W2073553052","https://openalex.org/W2618214196","https://openalex.org/W1749026082","https://openalex.org/W2105089251","https://openalex.org/W2504465921","https://openalex.org/W2249234990","https://openalex.org/W2082755851","https://openalex.org/W1877857937","https://openalex.org/W7006107223"],"abstract_inverted_index":{"When":[0],"round":[1,84,143,258],"cells":[2,17,60,86,145,316],"from":[3,132,272],"a":[4,112,153,193,206,243,282,321],"carrot":[5,85,107,144,165,259,283],"cell":[6,45,137,207],"suspension":[7],"culture":[8],"are":[9,290,340],"diluted":[10],"into":[11,195],"fresh":[12,275],"medium":[13],"without":[14],"auxin,":[15],"the":[16,39,42,53,74,122,181,186,196,199,212,215,219,235,239,248,252,255,278,307,310,318],"elongate":[18],"to":[19,121,180,247,302,325,332],"almost":[20],"50":[21],"times":[22],"their":[23],"original":[24],"diameter":[25],"within":[26],"three":[27],"days.":[28],"This":[29],"process":[30],"of":[31,44,55,83,91,105,116,124,135,142,163,174,183,185,198,214,221,229,238,251,257,268,277,281,312,314,320],"elongation":[32,184],"is":[33,263],"accompanied":[34],"by":[35,61,73,191],"changes":[36],"in":[37,58,109,152,234,292,305,343],"both":[38,306],"composition":[40,308],"and":[41,68,94,150,231,309,324,338],"orientation":[43,54,90,115,173,245,267],"wall":[46,56,208,288,345],"polymers.":[47,269],"We":[48],"have":[49,87,298],"obtained":[50,72,131,271],"information":[51],"on":[52,211],"polymers":[57,328],"elongating":[59,315],"two":[62],"complementary":[63],"techniques,":[64],"one":[65,69],"using":[66],"microscopy":[67],"spectroscopy.":[70],"Images":[71],"fast-freeze,":[75],"deep-etch,":[76],"rotary-shadowed":[77],"replica":[78],"technique":[79],"show":[80,111,139,242,285],"that":[81,95,140,167,286,327],"walls":[82,138,141,162,220,256,313],"no":[88,264],"net":[89,114,244,266],"cellulose":[92],"microfibrils,":[93],"many":[96],"thin":[97],"fibres":[98],"can":[99,188],"be":[100,189,333],"seen":[101],"cross-linking":[102],"microfibrils.":[103],"Walls":[104],"elongated":[106,164,222],"cells,":[108,166,223,260],"contrast,":[110],"marked":[113],"microfibrils":[117],"at":[118,317],"right":[119],"angles":[120],"axis":[123,250],"elongation.":[125],"Fourier":[126],"Transform":[127],"Infrared":[128],"(FTIR)":[129],"spectra":[130],"defined":[133],"areas":[134],"single":[136,322],"contain":[146,168],"more":[147,170],"protein,":[148,339],"esters":[149],"phenolics":[151],"given":[154],"area":[155],"(10":[156],"microns":[157],"x":[158],"10":[159],"microns)":[160],"than":[161],"proportionally":[169],"carbohydrate.":[171],"The":[172],"particular":[175],"functional":[176],"groups,":[177],"with":[178],"respect":[179],"direction":[182],"cell,":[187,323],"determined":[190],"inserting":[192],"polariser":[194],"path":[197],"infrared":[200],"beam,":[201],"before":[202],"it":[203],"passes":[204],"through":[205],"sample":[209],"mounted":[210],"stage":[213],"microscope":[216],"accessory.":[217],"In":[218,254],"ester":[224],"bands,":[225],"amide":[226],"bands":[227],"characteristic":[228],"proteins,":[230],"stretching":[232],"frequencies":[233],"carbohydrate":[236],"region":[237],"spectrum":[240],"all":[241],"transverse":[246],"long":[249],"cells.":[253],"however,":[261],"there":[262],"such":[265,335],"Spectra":[270],"25":[273],"microns-thick":[274],"sections":[276],"etiolated":[279],"stem":[280],"seedling":[284],"different":[287,293],"components":[289],"polarised":[291],"tissue":[294],"types.":[295,346],"These":[296],"techniques":[297],"therefore":[299],"enabled":[300],"us":[301],"define":[303],"differences":[304],"architecture":[311],"level":[319],"suggest":[326],"not":[329],"previously":[330],"thought":[331],"ordered,":[334],"as":[336],"pectin":[337],"strictly":[341],"oriented":[342],"some":[344]},"counts_by_year":[{"year":2023,"cited_by_count":3},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":3},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":2}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}