{"id":"https://openalex.org/W2156074545","doi":"https://doi.org/10.1111/j.1365-2958.1994.tb01272.x","title":"Molecular characterization of catalase from Bordetella pertussis: identification of the katA promoter in an upstream insertion sequence","display_name":"Molecular characterization of catalase from Bordetella pertussis: identification of the katA promoter in an upstream insertion sequence","publication_year":1994,"publication_date":"1994-10-01","ids":{"openalex":"https://openalex.org/W2156074545","doi":"https://doi.org/10.1111/j.1365-2958.1994.tb01272.x","mag":"2156074545","pmid":"https://pubmed.ncbi.nlm.nih.gov/7830550"},"language":"en","primary_location":{"id":"doi:10.1111/j.1365-2958.1994.tb01272.x","doi":"https://doi.org/10.1111/j.1365-2958.1994.tb01272.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1365-2958.1994.tb01272.x","pdf_url":null,"source":{"id":"https://openalex.org/S102549058","display_name":"Molecular Microbiology","issn_l":"0950-382X","issn":["0950-382X","1365-2958"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular Microbiology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5000934588","display_name":"David DeShazer","orcid":"https://orcid.org/0000-0003-1758-1238","observed_orcids":["https://orcid.org/0000-0003-1758-1238"]},"institutions":[{"id":"https://openalex.org/I138006243","display_name":"University of Arizona","ror":"https://ror.org/03m2x1q45","country_code":"US","type":"education","lineage":["https://openalex.org/I138006243"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"David DeShazer","raw_affiliation_strings":["Department of Microbiology and Immunology, University of Arizona, Tucson 85724","Department of Microbiology and Immunology, University of Arizona, Tucson, Arizona 85724, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology and Immunology, University of Arizona, Tucson 85724","institution_ids":["https://openalex.org/I138006243"]},{"raw_affiliation_string":"Department of Microbiology and Immunology, University of Arizona, Tucson, Arizona 85724, USA","institution_ids":["https://openalex.org/I138006243"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5087864446","display_name":"Gwendolyn E. Wood","orcid":"https://orcid.org/0000-0001-5109-3794","observed_orcids":["https://orcid.org/0000-0001-5109-3794"]},"institutions":[{"id":"https://openalex.org/I138006243","display_name":"University of Arizona","ror":"https://ror.org/03m2x1q45","country_code":"US","type":"education","lineage":["https://openalex.org/I138006243"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Gwendolyn E. Wood","raw_affiliation_strings":["Department of Microbiology and Immunology, University of Arizona, Tucson, Arizona 85724, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology and Immunology, University of Arizona, Tucson, Arizona 85724, USA","institution_ids":["https://openalex.org/I138006243"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5111867160","display_name":"Richard L. Friedman","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I138006243","display_name":"University of Arizona","ror":"https://ror.org/03m2x1q45","country_code":"US","type":"education","lineage":["https://openalex.org/I138006243"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Richard L. Friedman","raw_affiliation_strings":["Department of Microbiology and Immunology, University of Arizona, Tucson, Arizona 85724, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology and Immunology, University of Arizona, Tucson, Arizona 85724, USA","institution_ids":["https://openalex.org/I138006243"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5111867160"],"corresponding_institution_ids":["https://openalex.org/I138006243"],"apc_list":{"value":4430,"currency":"USD","value_usd":4430},"apc_paid":null,"fwci":2.8521,"has_fulltext":false,"cited_by_count":40,"citation_normalized_percentile":{"value":0.89366859,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"14","issue":"1","first_page":"123","last_page":"130"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10873","display_name":"Bacterial Infections and Vaccines","score":0.5414999723434448,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10873","display_name":"Bacterial Infections and Vaccines","score":0.5414999723434448,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.3379000127315521,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11884","display_name":"Redox biology and oxidative stress","score":0.03460000082850456,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/bordetella-pertussis","display_name":"Bordetella pertussis","score":0.9819999933242798},{"id":"https://openalex.org/keywords/catalase","display_name":"catalase","score":0.9860000014305115},{"id":"https://openalex.org/keywords/kata","display_name":"kata","score":0.753000020980835},{"id":"https://openalex.org/keywords/promoter-mapping","display_name":"promoter mapping","score":0.6129999756813049},{"id":"https://openalex.org/keywords/transcription-start-site","display_name":"transcription start sites","score":0.6869999766349792},{"id":"https://openalex.org/keywords/complementation","display_name":"complementation","score":0.6150000095367432},{"id":"https://openalex.org/keywords/hydrogen-peroxide-sensitivity","display_name":"hydrogen peroxide sensitivity","score":0.843999981880188},{"id":"https://openalex.org/keywords/polymorphonuclear-leukocytes","display_name":"polymorphonuclear leukocytes","score":0.8830000162124634},{"id":"https://openalex.org/keywords/bacterial-survival-in-host-cells","display_name":"bacterial survival in host cells","score":0.7089999914169312},{"id":"https://openalex.org/keywords/gene-replacement","display_name":"gene replacement","score":0.5479999780654907}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8500255346298218},{"id":"https://openalex.org/C2777582894","wikidata":"https://www.wikidata.org/wiki/Q137103","display_name":"Bordetella pertussis","level":3,"score":0.8324985504150391},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.6151890158653259},{"id":"https://openalex.org/C2778979269","wikidata":"https://www.wikidata.org/wiki/Q106951292","display_name":"Catalase","level":3,"score":0.5786465406417847},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.5771374702453613},{"id":"https://openalex.org/C84148353","wikidata":"https://www.wikidata.org/wiki/Q863908","display_name":"Nucleic acid sequence","level":3,"score":0.5724700093269348},{"id":"https://openalex.org/C194857396","wikidata":"https://www.wikidata.org/wiki/Q382878","display_name":"Primer extension","level":4,"score":0.5123704671859741},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.45261555910110474},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3195990025997162},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.26486313343048096},{"id":"https://openalex.org/C512185932","wikidata":"https://www.wikidata.org/wiki/Q28745","display_name":"Nucleotide","level":3,"score":0.2485080063343048},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.22270840406417847},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.20978745818138123},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.20693537592887878}],"mesh":[{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001886","descriptor_name":"Bordetella pertussis","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D001886","descriptor_name":"Bordetella pertussis","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D001886","descriptor_name":"Bordetella pertussis","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D002374","descriptor_name":"Catalase","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D002374","descriptor_name":"Catalase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D002374","descriptor_name":"Catalase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004251","descriptor_name":"DNA Transposable Elements","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D005798","descriptor_name":"Genes, Bacterial","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006861","descriptor_name":"Hydrogen Peroxide","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008826","descriptor_name":"Microbial Sensitivity Tests","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009504","descriptor_name":"Neutrophils","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":false},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011401","descriptor_name":"Promoter Regions, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D014158","descriptor_name":"Transcription, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015183","descriptor_name":"Restriction Mapping","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017353","descriptor_name":"Gene Deletion","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017931","descriptor_name":"DNA Primers","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1111/j.1365-2958.1994.tb01272.x","doi":"https://doi.org/10.1111/j.1365-2958.1994.tb01272.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1365-2958.1994.tb01272.x","pdf_url":null,"source":{"id":"https://openalex.org/S102549058","display_name":"Molecular Microbiology","issn_l":"0950-382X","issn":["0950-382X","1365-2958"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular Microbiology","raw_type":"journal-article"},{"id":"pmid:7830550","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7830550","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular microbiology","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.504800021648407,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":40,"referenced_works":["https://openalex.org/W87435791","https://openalex.org/W191793640","https://openalex.org/W1022042346","https://openalex.org/W1487254759","https://openalex.org/W1545276862","https://openalex.org/W1552890707","https://openalex.org/W1566044277","https://openalex.org/W1698425754","https://openalex.org/W1827240104","https://openalex.org/W1891988303","https://openalex.org/W1900978828","https://openalex.org/W1910268468","https://openalex.org/W1927634469","https://openalex.org/W1975085668","https://openalex.org/W2000055267","https://openalex.org/W2000559951","https://openalex.org/W2007457312","https://openalex.org/W2017365299","https://openalex.org/W2027229485","https://openalex.org/W2028714053","https://openalex.org/W2029514347","https://openalex.org/W2055622788","https://openalex.org/W2056613668","https://openalex.org/W2058007274","https://openalex.org/W2071872098","https://openalex.org/W2086556149","https://openalex.org/W2088084109","https://openalex.org/W2094475419","https://openalex.org/W2095225277","https://openalex.org/W2099506873","https://openalex.org/W2105553868","https://openalex.org/W2121420503","https://openalex.org/W2126929085","https://openalex.org/W2131495198","https://openalex.org/W2138270253","https://openalex.org/W2156166501","https://openalex.org/W2167513124","https://openalex.org/W2172109855","https://openalex.org/W2398280244","https://openalex.org/W2418639512"],"related_works":["https://openalex.org/W818254209","https://openalex.org/W1979989784","https://openalex.org/W2006287747","https://openalex.org/W74809193","https://openalex.org/W2135817401","https://openalex.org/W1970864527","https://openalex.org/W2027518048","https://openalex.org/W1819368333","https://openalex.org/W2027933751","https://openalex.org/W2017365299"],"abstract_inverted_index":{"In":[0],"this":[1],"report":[2],"we":[3],"evaluate":[4],"the":[5,10,87,94,159,169,192],"role":[6,190],"of":[7,12,22,52,57,63,76,86,91,96,99,109,111,137,171,194],"catalase":[8,28,40,186],"in":[9,80,119,168,180,191],"survival":[11,193],"Bordetella":[13],"pertussis":[14,24,46,128,196],"within":[15,197],"human":[16,181],"polymorphonuclear":[17],"leukocytes":[18],"(PMNs).":[19],"Crude":[20],"extracts":[21],"B.":[23,45,102,127,138,195],"exhibited":[25],"a":[26,54,66,74,97,101,116,131],"single":[27],"activity":[29],"when":[30],"subjected":[31],"to":[32,115,150,175],"non-denaturing":[33],"polyacrylamide":[34],"gel":[35],"electrophoresis":[36],"and":[37,83,130,154,173],"assayed":[38],"for":[39,177],"activity.":[41],"A":[42,134],"plasmid":[43],"containing":[44],"katA":[47,64,92,112],"was":[48,113,141,147,165],"identified":[49],"by":[50,121,143,152],"complementation":[51],"UM255,":[53],"catalase-deficient":[55,135],"strain":[56,136],"Escherichia":[58],"coli.":[59],"The":[60,106],"nucleotide":[61,88],"sequence":[62,89],"predicts":[65],"55":[67],"kDa":[68],"protein":[69],"that":[70,185],"shares":[71],"homology":[72],"with":[73,126],"class":[75],"haem-containing":[77],"catalases":[78],"found":[79],"both":[81],"eubacteria":[82],"eukaryotes.":[84],"Analysis":[85],"upstream":[90],"revealed":[93],"presence":[95],"copy":[98],"IS481,":[100],"pertussis-specific":[103],"insertion":[104],"sequence.":[105],"start":[107],"site":[108],"transcription":[110],"mapped":[114],"T":[117],"residue":[118],"IS481":[120],"primer":[122],"extension":[123],"analysis":[124],"performed":[125],"RNA":[129],"katA-specific":[132],"primer.":[133],"pertussis,":[139],"DD900,":[140],"constructed":[142],"gene":[144],"replacement.":[145],"DD900":[146,172],"more":[148],"sensitive":[149],"killing":[151],"1":[153],"5":[155],"mM":[156],"H2O2":[157],"than":[158],"parental":[160],"strain,":[161],"BP339.":[162],"However,":[163],"there":[164],"no":[166,188],"difference":[167],"ability":[170],"BP339":[174],"survive":[176],"2":[178],"h":[179],"PMNs.":[182,198],"This":[183],"suggests":[184],"plays":[187],"significant":[189]},"counts_by_year":[{"year":2025,"cited_by_count":2},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}