{"id":"https://openalex.org/W2155779574","doi":"https://doi.org/10.1128/mcb.20.3.883-891.2000","title":"Trypanosoma brucei Guide RNA Poly(U) Tail Formation Is Stabilized by Cognate mRNA","display_name":"Trypanosoma brucei Guide RNA Poly(U) Tail Formation Is Stabilized by Cognate mRNA","publication_year":2000,"publication_date":"2000-02-01","ids":{"openalex":"https://openalex.org/W2155779574","doi":"https://doi.org/10.1128/mcb.20.3.883-891.2000","mag":"2155779574","pmid":"https://pubmed.ncbi.nlm.nih.gov/10629045","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/85205"},"language":"en","primary_location":{"id":"doi:10.1128/mcb.20.3.883-891.2000","doi":"https://doi.org/10.1128/mcb.20.3.883-891.2000","is_oa":false,"landing_page_url":"https://doi.org/10.1128/mcb.20.3.883-891.2000","pdf_url":null,"source":{"id":"https://openalex.org/S81455967","display_name":"Molecular and Cellular Biology","issn_l":"0270-7306","issn":["0270-7306","1067-8824","1098-5549"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320547","host_organization_name":"Taylor & Francis","host_organization_lineage":["https://openalex.org/P4310320547","https://openalex.org/P4310320449"],"host_organization_lineage_names":["Taylor & Francis","Informa"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular and Cellular Biology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/85205","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5039780162","display_name":"Michael T. McManus","orcid":"https://orcid.org/0000-0003-3013-6569","observed_orcids":["https://orcid.org/0000-0003-3013-6569"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Michael T. McManus","raw_affiliation_strings":["Department of Biochemistry and Molecular Genetics"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Molecular Genetics","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5046988732","display_name":"Brian K. Adler","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I32389192","display_name":"University of Alabama at Birmingham","ror":"https://ror.org/008s83205","country_code":"US","type":"education","lineage":["https://openalex.org/I32389192"]},{"id":"https://openalex.org/I4390039250","display_name":"UAB Medicine","ror":"https://ror.org/036554539","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4390039250"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Brian K. Adler","raw_affiliation_strings":["Department of Medicine, School of Medicine, University of Alabama at Birmingham","Department of Medicine, School of Medicine, University of Alabama at Birmingham, Birmingham, Alabama 35294"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, School of Medicine, University of Alabama at Birmingham","institution_ids":["https://openalex.org/I32389192"]},{"raw_affiliation_string":"Department of Medicine, School of Medicine, University of Alabama at Birmingham, Birmingham, Alabama 35294","institution_ids":["https://openalex.org/I32389192","https://openalex.org/I4390039250"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5091102083","display_name":"Victoria W. Pollard","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I79576946","display_name":"University of Pennsylvania","ror":"https://ror.org/00b30xv10","country_code":"US","type":"education","lineage":["https://openalex.org/I79576946"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Victoria W. Pollard","raw_affiliation_strings":["Department of Microbiology, School of Medicine, University of Pennsylvania","Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6148"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, School of Medicine, University of Pennsylvania","institution_ids":["https://openalex.org/I79576946"]},{"raw_affiliation_string":"Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6148","institution_ids":["https://openalex.org/I79576946"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5023909283","display_name":"Stephen L. Hajduk","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Stephen L. Hajduk","raw_affiliation_strings":["Department of Biochemistry and Molecular Genetics"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Molecular Genetics","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5023909283"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":3.7451,"has_fulltext":false,"cited_by_count":40,"citation_normalized_percentile":{"value":0.92907656,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":"20","issue":"3","first_page":"883","last_page":"891"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10890","display_name":"Trypanosoma species research and implications","score":0.9740999937057495,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10890","display_name":"Trypanosoma species research and implications","score":0.9740999937057495,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12610","display_name":"RNA regulation and disease","score":0.014600000344216824,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10604","display_name":"RNA Research and Splicing","score":0.00839999970048666,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/trypanosoma-brucei","display_name":"Trypanosoma brucei","score":0.9860000014305115},{"id":"https://openalex.org/keywords/guide-rna","display_name":"guide RNA","score":0.8640000224113464},{"id":"https://openalex.org/keywords/rna-editing","display_name":"RNA editing","score":0.8659999966621399},{"id":"https://openalex.org/keywords/ribonucleoprotein-complexes","display_name":"ribonucleoprotein complexes","score":0.8040000200271606},{"id":"https://openalex.org/keywords/3-exonuclease","display_name":"3' exonuclease","score":0.906000018119812},{"id":"https://openalex.org/keywords/mitochondrial-rna-editing","display_name":"mitochondrial RNA editing","score":0.34299999475479126}],"concepts":[{"id":"https://openalex.org/C97702854","wikidata":"https://www.wikidata.org/wiki/Q4039747","display_name":"Guide RNA","level":5,"score":0.7860004901885986},{"id":"https://openalex.org/C21786251","wikidata":"https://www.wikidata.org/wiki/Q14886350","display_name":"RNA editing","level":4,"score":0.7147707939147949},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7113196849822998},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.6037454605102539},{"id":"https://openalex.org/C2777501222","wikidata":"https://www.wikidata.org/wiki/Q411664","display_name":"Exonuclease","level":4,"score":0.5794094800949097},{"id":"https://openalex.org/C2781259782","wikidata":"https://www.wikidata.org/wiki/Q422573","display_name":"Uridine","level":4,"score":0.49380120635032654},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.46993952989578247},{"id":"https://openalex.org/C75934600","wikidata":"https://www.wikidata.org/wiki/Q419245","display_name":"Ribonucleoprotein","level":4,"score":0.4558377265930176},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4529132843017578},{"id":"https://openalex.org/C41282012","wikidata":"https://www.wikidata.org/wiki/Q3928341","display_name":"RNA-binding protein","level":4,"score":0.437509685754776},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.42640557885169983},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.21793264150619507},{"id":"https://openalex.org/C82381507","wikidata":"https://www.wikidata.org/wiki/Q416878","display_name":"Polymerase","level":3,"score":0.1383981704711914},{"id":"https://openalex.org/C98108389","wikidata":"https://www.wikidata.org/wiki/Q412563","display_name":"CRISPR","level":3,"score":0.13691741228103638},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.12426215410232544},{"id":"https://openalex.org/C132455925","wikidata":"https://www.wikidata.org/wiki/Q16965677","display_name":"Cas9","level":4,"score":0.080567866563797}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008928","descriptor_name":"Mitochondria","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011072","descriptor_name":"Poly U","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D012261","descriptor_name":"Ribonucleoproteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D012316","descriptor_name":"RNA Nucleotidyltransferases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012322","descriptor_name":"RNA Precursors","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012322","descriptor_name":"RNA Precursors","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012323","descriptor_name":"RNA Processing, Post-Transcriptional","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D014346","descriptor_name":"Trypanosoma brucei brucei","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D016053","descriptor_name":"RNA, Protozoan","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D016053","descriptor_name":"RNA, Protozoan","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D017393","descriptor_name":"RNA Editing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017394","descriptor_name":"RNA, Guide, Kinetoplastida","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D017394","descriptor_name":"RNA, Guide, Kinetoplastida","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1128/mcb.20.3.883-891.2000","doi":"https://doi.org/10.1128/mcb.20.3.883-891.2000","is_oa":false,"landing_page_url":"https://doi.org/10.1128/mcb.20.3.883-891.2000","pdf_url":null,"source":{"id":"https://openalex.org/S81455967","display_name":"Molecular and Cellular Biology","issn_l":"0270-7306","issn":["0270-7306","1067-8824","1098-5549"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320547","host_organization_name":"Taylor & Francis","host_organization_lineage":["https://openalex.org/P4310320547","https://openalex.org/P4310320449"],"host_organization_lineage_names":["Taylor & Francis","Informa"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular and Cellular Biology","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:85205","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/85205","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Mol Cell Biol","raw_type":"Text"},{"id":"pmid:10629045","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10629045","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular and cellular biology","raw_type":"Journal Article"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.324.5552","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.324.5552","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://mcb.asm.org/content/20/3/883.full.pdf","raw_type":"text"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:85205","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/85205","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Mol Cell Biol","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2409791582","display_name":"DEVELOPMENTAL REGULATION AND PROCESSING OF RIBOSOMAL RNA","funder_award_id":"5k11ca060151-03","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G3133749362","display_name":null,"funder_award_id":"AI 21401","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"},{"id":"https://openalex.org/G4392718754","display_name":"Function of mRNA Editing in Trypanosomes","funder_award_id":"R01AI021401","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"},{"id":"https://openalex.org/G539426713","display_name":null,"funder_award_id":"CA60151","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"},{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":30,"referenced_works":["https://openalex.org/W131501599","https://openalex.org/W655523946","https://openalex.org/W1534643095","https://openalex.org/W1549086518","https://openalex.org/W1607243293","https://openalex.org/W1893369488","https://openalex.org/W1963159333","https://openalex.org/W1974939251","https://openalex.org/W1977764715","https://openalex.org/W1980730222","https://openalex.org/W1981419683","https://openalex.org/W1993382008","https://openalex.org/W2013022956","https://openalex.org/W2023775661","https://openalex.org/W2046703384","https://openalex.org/W2072777207","https://openalex.org/W2076628226","https://openalex.org/W2086671276","https://openalex.org/W2097663462","https://openalex.org/W2098349971","https://openalex.org/W2102106816","https://openalex.org/W2105344150","https://openalex.org/W2114306163","https://openalex.org/W2128972342","https://openalex.org/W2144176758","https://openalex.org/W2145594096","https://openalex.org/W2157243930","https://openalex.org/W2160178067","https://openalex.org/W2264429446","https://openalex.org/W2341418583"],"related_works":["https://openalex.org/W2114306163","https://openalex.org/W2122387372","https://openalex.org/W2160178067","https://openalex.org/W2016413360","https://openalex.org/W2051023465","https://openalex.org/W2085444198","https://openalex.org/W2169125466","https://openalex.org/W2072777207","https://openalex.org/W2136645624","https://openalex.org/W2416397439"],"abstract_inverted_index":{"Guide":[0],"RNAs":[1,5,116,140],"(gRNAs)":[2],"are":[3,136],"small":[4],"that":[6,145,198],"provide":[7],"specificity":[8],"for":[9,92,248],"uridine":[10,191,195,234],"addition":[11,112],"and":[12,30,57,66,88,242],"deletion":[13],"during":[14,27,53],"mRNA":[15,52,87,173,188,254],"editing":[16,29,81],"in":[17,101,180,255],"trypanosomes.":[18],"Terminal":[19],"uridylyl":[20],"transferase":[21],"(TUTase)":[22],"adds":[23,31],"uridines":[24,135,160],"to":[25,35,69,75,113,138,217,231],"pre-mRNAs":[26],"RNA":[28,229],"a":[32,127,142,178,194,246],"poly(U)":[33,42,223],"tail":[34,43,192,224,235],"the":[36,46,77,102,120,124,155,171,182,186,190,202,211,215,222,226,233,237,249,253],"3'":[37,129,157],"end":[38],"of":[39,48,95,104,126,151,185,214,225,236,252],"gRNAs.":[40],"The":[41],"may":[44,146],"stabilize":[45],"association":[47],"gRNAs":[49,58,166],"with":[50,60,221,245],"cognate":[51,172,187],"editing.":[54,93],"Both":[55],"TUTase":[56,96,108],"associate":[59],"two":[61,105],"ribonucleoprotein":[62],"complexes,":[63],"I":[64,153,169],"(19S)":[65],"II":[67,72],"(35S":[68],"40S).":[70],"Complex":[71],"is":[73,117,174,199,243],"believed":[74],"be":[76,147,162],"fully":[78],"assembled":[79],"active":[80],"complex,":[82,122],"since":[83],"it":[84],"contains":[85],"pre-edited":[86],"enzymes":[89],"thought":[90],"necessary":[91],"Purification":[94],"from":[97,193,239],"mitochondrial":[98],"extracts":[99],"resulted":[100],"identification":[103],"chromatographically":[106],"distinct":[107],"activities.":[109],"Stable":[110],"single-uridine":[111],"different":[114],"substrate":[115,139],"performed":[118],"by":[119,141,167],"19S":[121],"despite":[123],"presence":[125],"uridine-specific":[128,156],"exonuclease":[130,196],"within":[131,201],"this":[132,206],"complex.":[133,203],"Multiple":[134,159],"added":[137,164],"10S":[143],"particle":[144],"an":[148],"unstable":[149],"subunit":[150],"complex":[152,168],"lacking":[154],"exonuclease.":[158],"could":[161],"stably":[163],"onto":[165],"when":[170],"present.":[175],"We":[176],"propose":[177],"model":[179],"which":[181],"purine-rich":[183,212,250],"region":[184,213,251],"protects":[189],"activity":[197],"present":[200],"To":[204],"test":[205],"model,":[207],"we":[208],"have":[209],"mutated":[210],"pre-mRNA":[216],"abolish":[218],"base-pairing":[219],"interaction":[220],"gRNA.":[227],"This":[228],"fails":[230],"protect":[232],"gRNA":[238,256],"exoribonucleolytic":[240],"trimming":[241],"consistent":[244],"role":[247],"maturation.":[257]},"counts_by_year":[{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}