{"id":"https://openalex.org/W2155299121","doi":"https://doi.org/10.2337/diab.31.7.659","title":"Direct Demonstration of Insulin Receptor Internalization: A Quantitative Electron Microscopic Study of Covalently Bound 125I-photoreactive Insulin Incubated with Isolated Hepatocytes","display_name":"Direct Demonstration of Insulin Receptor Internalization: A Quantitative Electron Microscopic Study of Covalently Bound 125I-photoreactive Insulin Incubated with Isolated Hepatocytes","publication_year":1982,"publication_date":"1982-07-01","ids":{"openalex":"https://openalex.org/W2155299121","doi":"https://doi.org/10.2337/diab.31.7.659","mag":"2155299121","pmid":"https://pubmed.ncbi.nlm.nih.gov/6761204"},"language":"en","primary_location":{"id":"doi:10.2337/diab.31.7.659","doi":"https://doi.org/10.2337/diab.31.7.659","is_oa":false,"landing_page_url":"https://doi.org/10.2337/diab.31.7.659","pdf_url":null,"source":{"id":"https://openalex.org/S129060628","display_name":"Diabetes","issn_l":"0012-1797","issn":["0012-1797","1939-327X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320274","host_organization_name":"American Diabetes Association","host_organization_lineage":["https://openalex.org/P4310320274"],"host_organization_lineage_names":["American Diabetes Association"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Diabetes","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5070064147","display_name":"Phillip G\u00f6rden","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210090567","display_name":"National Institute of Diabetes and Digestive and Kidney Diseases","ror":"https://ror.org/00adh9b73","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210090567"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Phillip Gorden","raw_affiliation_strings":["Diabetes Branch, National Institute of Arthritis, Diabetes, and Digestive and Kidney Diseases, National Institutes of Health\u2008Bethesda, Maryland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Diabetes Branch, National Institute of Arthritis, Diabetes, and Digestive and Kidney Diseases, National Institutes of Health\u2008Bethesda, Maryland","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210090567"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113671278","display_name":"Jean\u2010Louis Carpentier","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I114457229","display_name":"University of Geneva","ror":"https://ror.org/01swzsf04","country_code":"CH","type":"education","lineage":["https://openalex.org/I114457229"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"Jean-Louis Carpentier","raw_affiliation_strings":["Institute of Histology and Embryology, University of Geneva Medical School\u2008Geneva, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Histology and Embryology, University of Geneva Medical School\u2008Geneva, Switzerland","institution_ids":["https://openalex.org/I114457229"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5063492206","display_name":"Margaret L. Moule","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I185261750","display_name":"University of Toronto","ror":"https://ror.org/03dbr7087","country_code":"CA","type":"education","lineage":["https://openalex.org/I185261750"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Margaret L Moule","raw_affiliation_strings":["The Banting and Best Department of Medical Research, University of Toronto\u2008Toronto, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Banting and Best Department of Medical Research, University of Toronto\u2008Toronto, Canada","institution_ids":["https://openalex.org/I185261750"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112739952","display_name":"Cecil C. Yip","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I185261750","display_name":"University of Toronto","ror":"https://ror.org/03dbr7087","country_code":"CA","type":"education","lineage":["https://openalex.org/I185261750"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Cecil C Yip","raw_affiliation_strings":["The Banting and Best Department of Medical Research, University of Toronto\u2008Toronto, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Banting and Best Department of Medical Research, University of Toronto\u2008Toronto, Canada","institution_ids":["https://openalex.org/I185261750"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5034171652","display_name":"Lelio Orci","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I114457229","display_name":"University of Geneva","ror":"https://ror.org/01swzsf04","country_code":"CH","type":"education","lineage":["https://openalex.org/I114457229"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"Lelio Orci","raw_affiliation_strings":["Institute of Histology and Embryology, University of Geneva Medical School\u2008Geneva, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Histology and Embryology, University of Geneva Medical School\u2008Geneva, Switzerland","institution_ids":["https://openalex.org/I114457229"]}]}],"institutions":[],"countries_distinct_count":3,"institutions_distinct_count":4,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":2.6994,"has_fulltext":false,"cited_by_count":30,"citation_normalized_percentile":{"value":0.9050301,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":null,"biblio":{"volume":"31","issue":"7","first_page":"659","last_page":"662"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10617","display_name":"Cellular transport and secretion","score":0.9057999849319458,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10617","display_name":"Cellular transport and secretion","score":0.9057999849319458,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11339","display_name":"Metabolism, Diabetes, and Cancer","score":0.035599999129772186,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11178","display_name":"Receptor Mechanisms and Signaling","score":0.02449999935925007,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/insulin-receptor-internalization","display_name":"insulin receptor internalization","score":0.9169999957084656},{"id":"https://openalex.org/keywords/receptor-internalization","display_name":"receptor internalization","score":0.6299999952316284},{"id":"https://openalex.org/keywords/adsorptive-endocytosis","display_name":"adsorptive endocytosis","score":0.8309999704360962},{"id":"https://openalex.org/keywords/hepatocytes","display_name":"hepatocytes","score":0.7229999899864197},{"id":"https://openalex.org/keywords/quantitative-electron-microscopy","display_name":"quantitative electron microscopy","score":0.5120000243186951},{"id":"https://openalex.org/keywords/autoradiography","display_name":"autoradiography","score":0.722000002861023},{"id":"https://openalex.org/keywords/ligand-receptor-binding","display_name":"ligand-receptor binding","score":0.628000020980835}],"concepts":[{"id":"https://openalex.org/C139770010","wikidata":"https://www.wikidata.org/wiki/Q1339807","display_name":"Internalization","level":3,"score":0.9429388642311096},{"id":"https://openalex.org/C28005876","wikidata":"https://www.wikidata.org/wiki/Q189814","display_name":"Endocytosis","level":3,"score":0.8102259635925293},{"id":"https://openalex.org/C2779306644","wikidata":"https://www.wikidata.org/wiki/Q2002370","display_name":"Insulin","level":2,"score":0.7724120020866394},{"id":"https://openalex.org/C112446052","wikidata":"https://www.wikidata.org/wiki/Q422579","display_name":"Insulin receptor","level":4,"score":0.6689465641975403},{"id":"https://openalex.org/C180577832","wikidata":"https://www.wikidata.org/wiki/Q127920","display_name":"Covalent bond","level":2,"score":0.6643463373184204},{"id":"https://openalex.org/C116569031","wikidata":"https://www.wikidata.org/wiki/Q899107","display_name":"Ligand (biochemistry)","level":3,"score":0.6407666802406311},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.5456699132919312},{"id":"https://openalex.org/C2776200302","wikidata":"https://www.wikidata.org/wiki/Q827450","display_name":"Hepatocyte","level":3,"score":0.5337971448898315},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.44255369901657104},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4371100664138794},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3656404912471771},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.33284008502960205},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.23128870129585266},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.14049690961837769},{"id":"https://openalex.org/C2777391703","wikidata":"https://www.wikidata.org/wiki/Q1053470","display_name":"Insulin resistance","level":3,"score":0.09329888224601746},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001345","descriptor_name":"Autoradiography","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002462","descriptor_name":"Cell Membrane","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004705","descriptor_name":"Endocytosis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007328","descriptor_name":"Insulin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D007457","descriptor_name":"Iodine Radioisotopes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D008099","descriptor_name":"Liver","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008099","descriptor_name":"Liver","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":true},{"descriptor_ui":"D008854","descriptor_name":"Microscopy, Electron","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011972","descriptor_name":"Receptor, Insulin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.2337/diab.31.7.659","doi":"https://doi.org/10.2337/diab.31.7.659","is_oa":false,"landing_page_url":"https://doi.org/10.2337/diab.31.7.659","pdf_url":null,"source":{"id":"https://openalex.org/S129060628","display_name":"Diabetes","issn_l":"0012-1797","issn":["0012-1797","1939-327X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320274","host_organization_name":"American Diabetes Association","host_organization_lineage":["https://openalex.org/P4310320274"],"host_organization_lineage_names":["American Diabetes Association"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Diabetes","raw_type":"journal-article"},{"id":"doi:10.2337/diabetes.31.7.659","doi":"https://doi.org/10.2337/diabetes.31.7.659","is_oa":false,"landing_page_url":"https://doi.org/10.2337/diabetes.31.7.659","pdf_url":null,"source":{"id":"https://openalex.org/S129060628","display_name":"Diabetes","issn_l":"0012-1797","issn":["0012-1797","1939-327X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320274","host_organization_name":"American Diabetes Association","host_organization_lineage":["https://openalex.org/P4310320274"],"host_organization_lineage_names":["American Diabetes Association"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Diabetes","raw_type":"journal-article"},{"id":"pmid:6761204","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/6761204","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Diabetes","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.428600013256073,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320320924","display_name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","ror":"https://ror.org/00yjd3n13"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":13,"referenced_works":["https://openalex.org/W1490154674","https://openalex.org/W1525266731","https://openalex.org/W1631855017","https://openalex.org/W1970029702","https://openalex.org/W1972911473","https://openalex.org/W1974711732","https://openalex.org/W1979075448","https://openalex.org/W1979170193","https://openalex.org/W2046611375","https://openalex.org/W2047671656","https://openalex.org/W2064121091","https://openalex.org/W2092225525","https://openalex.org/W2883239271"],"related_works":["https://openalex.org/W7099845872","https://openalex.org/W2092225525","https://openalex.org/W7097075154","https://openalex.org/W1993111972","https://openalex.org/W1979075448","https://openalex.org/W2307904896","https://openalex.org/W2039850365","https://openalex.org/W4236817951","https://openalex.org/W1979170193","https://openalex.org/W2019019550"],"abstract_inverted_index":{"When":[0],"125I-insulin":[1],"is":[2,24,37,73,97],"incubated":[3],"with":[4,39,99],"isolated":[5],"rodent":[6],"hepatocytes":[7],"at":[8,89],"37":[9],"degrees":[10],"C,":[11],"the":[12,17,21,34,40,68,76,82,94,100],"ligand":[13],"initially":[14],"binds":[15],"to":[16,51,81],"plasma":[18],"membrane":[19],"of":[20,84],"cell":[22,77],"and":[23,56],"subsequently":[25],"internalized":[26,38,74,98],"by":[27,60,75],"adsorptive":[28],"endocytosis.":[29],"To":[30],"confirm":[31],"directly":[32],"that":[33,67],"insulin":[35,50,72,95],"receptor":[36,55,96],"ligand,":[41],"we":[42],"covalently":[43,69],"linked":[44,70],"photoreactive":[45,71],"125I-N":[46],"sigma":[47],"B29":[48],"(azidobenzoyl)":[49],"its":[52,58],"specific":[53],"hepatocyte":[54],"followed":[57],"fate":[59],"quantitative":[61],"electron":[62],"microscopic":[63],"autoradiography.":[64],"We":[65],"found":[66],"in":[78],"fashion":[79],"analogous":[80],"internalization":[83],"ordinary":[85],"125I-insulin,":[86],"indicating":[87],"that,":[88],"least":[90],"under":[91],"these":[92],"conditions,":[93],"ligand.":[101]},"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}