{"id":"https://openalex.org/W2154137725","doi":"https://doi.org/10.1074/jbc.m002404200","title":"Catalytic Peptide of Human Glutaminyl-tRNA Synthetase Is Essential for Its Assembly to the Aminoacyl-tRNA Synthetase Complex","display_name":"Catalytic Peptide of Human Glutaminyl-tRNA Synthetase Is Essential for Its Assembly to the Aminoacyl-tRNA Synthetase Complex","publication_year":2000,"publication_date":"2000-07-01","ids":{"openalex":"https://openalex.org/W2154137725","doi":"https://doi.org/10.1074/jbc.m002404200","mag":"2154137725","pmid":"https://pubmed.ncbi.nlm.nih.gov/10801842"},"language":"en","primary_location":{"id":"doi:10.1074/jbc.m002404200","doi":"https://doi.org/10.1074/jbc.m002404200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m002404200","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1074/jbc.m002404200","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5100452198","display_name":"Taeho Kim","orcid":"https://orcid.org/0000-0002-7500-8918","observed_orcids":["https://orcid.org/0000-0002-7500-8918"]},"institutions":[{"id":"https://openalex.org/I848706","display_name":"Sungkyunkwan University","ror":"https://ror.org/04q78tk20","country_code":"KR","type":"education","lineage":["https://openalex.org/I848706"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Taeho Kim","raw_affiliation_strings":["National Creative Research Initiatives Center for ARS Network, Sung Kyun Kwan University, Suwon, Kyunggido 440-746, Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Creative Research Initiatives Center for ARS Network, Sung Kyun Kwan University, Suwon, Kyunggido 440-746, Korea","institution_ids":["https://openalex.org/I848706"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101847178","display_name":"Sang Gyu Park","orcid":"https://orcid.org/0000-0002-4870-2242","observed_orcids":["https://orcid.org/0000-0002-4870-2242"]},"institutions":[{"id":"https://openalex.org/I848706","display_name":"Sungkyunkwan University","ror":"https://ror.org/04q78tk20","country_code":"KR","type":"education","lineage":["https://openalex.org/I848706"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Sang Gyu Park","raw_affiliation_strings":["National Creative Research Initiatives Center for ARS Network, Sung Kyun Kwan University, Suwon, Kyunggido 440-746, Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Creative Research Initiatives Center for ARS Network, Sung Kyun Kwan University, Suwon, Kyunggido 440-746, Korea","institution_ids":["https://openalex.org/I848706"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5082438051","display_name":"Jee Eun Kim","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I848706","display_name":"Sungkyunkwan University","ror":"https://ror.org/04q78tk20","country_code":"KR","type":"education","lineage":["https://openalex.org/I848706"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Jee Eun Kim","raw_affiliation_strings":["National Creative Research Initiatives Center for ARS Network, Sung Kyun Kwan University, Suwon, Kyunggido 440-746, Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Creative Research Initiatives Center for ARS Network, Sung Kyun Kwan University, Suwon, Kyunggido 440-746, Korea","institution_ids":["https://openalex.org/I848706"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5086083933","display_name":"Wongi Seol","orcid":"https://orcid.org/0000-0003-4702-9428","observed_orcids":["https://orcid.org/0000-0003-4702-9428"]},"institutions":[{"id":"https://openalex.org/I848706","display_name":"Sungkyunkwan University","ror":"https://ror.org/04q78tk20","country_code":"KR","type":"education","lineage":["https://openalex.org/I848706"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Wongi Seol","raw_affiliation_strings":["National Creative Research Initiatives Center for ARS Network, Sung Kyun Kwan University, Suwon, Kyunggido 440-746, Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Creative Research Initiatives Center for ARS Network, Sung Kyun Kwan University, Suwon, Kyunggido 440-746, Korea","institution_ids":["https://openalex.org/I848706"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113581452","display_name":"Young\u2010Gyu Ko","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I848706","display_name":"Sungkyunkwan University","ror":"https://ror.org/04q78tk20","country_code":"KR","type":"education","lineage":["https://openalex.org/I848706"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Young-Gyu Ko","raw_affiliation_strings":["National Creative Research Initiatives Center for ARS Network, Sung Kyun Kwan University, Suwon, Kyunggido 440-746, Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Creative Research Initiatives Center for ARS Network, Sung Kyun Kwan University, Suwon, Kyunggido 440-746, Korea","institution_ids":["https://openalex.org/I848706"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5100429324","display_name":"Sung\u2010Hoon Kim","orcid":"https://orcid.org/0000-0002-1570-3230","observed_orcids":["https://orcid.org/0000-0002-1570-3230"]},"institutions":[{"id":"https://openalex.org/I848706","display_name":"Sungkyunkwan University","ror":"https://ror.org/04q78tk20","country_code":"KR","type":"education","lineage":["https://openalex.org/I848706"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Sunghoon Kim","raw_affiliation_strings":["National Creative Research Initiatives Center for ARS Network, Sung Kyun Kwan University, Suwon, Kyunggido 440-746, Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Creative Research Initiatives Center for ARS Network, Sung Kyun Kwan University, Suwon, Kyunggido 440-746, Korea","institution_ids":["https://openalex.org/I848706"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I848706"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":0.8959,"has_fulltext":false,"cited_by_count":34,"citation_normalized_percentile":{"value":0.70967317,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"275","issue":"28","first_page":"21768","last_page":"21772"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11482","display_name":"RNA modifications and cancer","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12770","display_name":"Amino Acid Enzymes and Metabolism","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/glutaminyl-trna-synthetase","display_name":"glutaminyl-tRNA synthetase","score":0.9950000047683716},{"id":"https://openalex.org/keywords/protein-complex-assembly","display_name":"protein complex formation","score":0.7009999752044678},{"id":"https://openalex.org/keywords/catalytic-domain","display_name":"catalytic domain","score":0.9269999861717224},{"id":"https://openalex.org/keywords/rossman-fold","display_name":"Rossman fold","score":0.8740000128746033},{"id":"https://openalex.org/keywords/atp-binding-domain","display_name":"ATP-binding domain","score":0.7099999785423279},{"id":"https://openalex.org/keywords/arginyl-trna-synthetase","display_name":"arginyl-tRNA synthetase","score":0.9049999713897705},{"id":"https://openalex.org/keywords/p43","display_name":"p43","score":0.9480000138282776},{"id":"https://openalex.org/keywords/protein-protein-interaction","display_name":"protein-protein interaction","score":0.6589999794960022}],"concepts":[{"id":"https://openalex.org/C151238385","wikidata":"https://www.wikidata.org/wiki/Q4746444","display_name":"Aminoacylation","level":5,"score":0.7459960579872131},{"id":"https://openalex.org/C71829478","wikidata":"https://www.wikidata.org/wiki/Q901305","display_name":"Immunoprecipitation","level":3,"score":0.5978615283966064},{"id":"https://openalex.org/C153957851","wikidata":"https://www.wikidata.org/wiki/Q201448","display_name":"Transfer RNA","level":4,"score":0.5389360785484314},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.5293437242507935},{"id":"https://openalex.org/C2779315393","wikidata":"https://www.wikidata.org/wiki/Q415968","display_name":"Aminoacyl tRNA synthetase","level":5,"score":0.5219504237174988},{"id":"https://openalex.org/C111773187","wikidata":"https://www.wikidata.org/wiki/Q1969239","display_name":"QRS complex","level":2,"score":0.4946386516094208},{"id":"https://openalex.org/C174510640","wikidata":"https://www.wikidata.org/wiki/Q489618","display_name":"HEK 293 cells","level":3,"score":0.4901450574398041},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4889194071292877},{"id":"https://openalex.org/C16613235","wikidata":"https://www.wikidata.org/wiki/Q285982","display_name":"Endogeny","level":2,"score":0.44594091176986694},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4397783577442169},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3414992690086365},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3333374857902527},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.20118623971939087},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.10833126306533813},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.09779918193817139},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.09387078881263733}],"mesh":[{"descriptor_ui":"D000255","descriptor_name":"Adenosine Triphosphate","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000604","descriptor_name":"Amino Acyl-tRNA Synthetases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D000604","descriptor_name":"Amino Acyl-tRNA Synthetases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D000604","descriptor_name":"Amino Acyl-tRNA Synthetases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009097","descriptor_name":"Multienzyme Complexes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D009097","descriptor_name":"Multienzyme Complexes","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D014162","descriptor_name":"Transfection","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016296","descriptor_name":"Mutagenesis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017384","descriptor_name":"Sequence Deletion","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020134","descriptor_name":"Catalytic Domain","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1074/jbc.m002404200","doi":"https://doi.org/10.1074/jbc.m002404200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m002404200","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:10801842","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10801842","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1074/jbc.m002404200","doi":"https://doi.org/10.1074/jbc.m002404200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m002404200","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":22,"referenced_works":["https://openalex.org/W108259644","https://openalex.org/W1525033943","https://openalex.org/W1541138432","https://openalex.org/W1548980675","https://openalex.org/W1565738858","https://openalex.org/W1602665067","https://openalex.org/W1711342713","https://openalex.org/W1819124197","https://openalex.org/W1972640950","https://openalex.org/W1973121921","https://openalex.org/W2004955970","https://openalex.org/W2008039771","https://openalex.org/W2012063342","https://openalex.org/W2015956685","https://openalex.org/W2021970333","https://openalex.org/W2032080162","https://openalex.org/W2045445805","https://openalex.org/W2076544746","https://openalex.org/W2120843102","https://openalex.org/W2136990296","https://openalex.org/W2147919483","https://openalex.org/W2152164434"],"related_works":["https://openalex.org/W1985747127","https://openalex.org/W1973634405","https://openalex.org/W2024735723","https://openalex.org/W2971206228","https://openalex.org/W1991202844","https://openalex.org/W2021313226","https://openalex.org/W2089683534","https://openalex.org/W2957094631","https://openalex.org/W3164152639","https://openalex.org/W1971729732"],"abstract_inverted_index":{"Human":[0],"glutaminyl-tRNA":[1],"synthetase":[2,148,173],"(QRS)":[3],"is":[4,168,203],"one":[5],"of":[6,22,40,91,108,166,184,191],"several":[7],"mammalian":[8],"aminoacyl-tRNA":[9],"synthetases":[10],"(ARSs)":[11],"that":[12,82,162],"form":[13],"a":[14,52,67],"macromolecular":[15],"protein":[16],"complex.":[17,96,178,211],"To":[18],"understand":[19],"the":[20,26,57,64,72,83,94,117,128,133,154,163,177,180,189,195,199,209],"mechanism":[21],"QRS":[23,43,69,92,129,167,185,206],"targeting":[24,90],"to":[25,63,93,170,187],"multi-ARS":[27,65,95,155,196,210],"complex,":[28,66,197],"we":[29],"analyzed":[30],"both":[31,127],"exogenous":[32,98],"and":[33,132,149,174],"endogenous":[34,104,159],"QRSs":[35],"by":[36],"immunoprecipitation":[37],"after":[38],"overexpression":[39],"various":[41],"Myc-tagged":[42,99],"mutants":[44],"in":[45,113,194],"human":[46],"embryonic":[47],"kidney":[48],"293":[49],"cells.":[50],"Whereas":[51],"deletion":[53],"mutant":[54,68],"containing":[55,70],"only":[56,71],"catalytic":[58,130,201],"domain":[59,75,131,136,202],"(QRS-C)":[60],"was":[61,77,87,101,110,145],"targeted":[62],"N-terminal":[73,134,164,182],"appended":[74,135],"(QRS-N)":[76],"not.":[78],"Deletion":[79],"mapping":[80],"showed":[81],"ATP-binding":[84],"Rossman":[85],"fold":[86],"necessary":[88,204],"for":[89,139,205],"Furthermore,":[97],"QRS-C":[100,124,144],"co-immunoprecipitated":[102],"with":[103,116,122,158,208],"QRS.":[105],"Since":[106],"glutaminylation":[107],"tRNA":[109],"dramatically":[111],"increased":[112],"cells":[114],"transfected":[115],"full-length":[118],"QRS,":[119,160],"but":[120],"not":[121],"either":[123],"or":[125],"QRS-N,":[126],"were":[137,151],"required":[138,169],"full":[140],"aminoacylation":[141],"activity.":[142],"When":[143],"overexpressed,":[146],"arginyl-tRNA":[147,172],"p43":[150,175],"released":[152],"from":[153],"complex":[156],"along":[157],"suggesting":[161],"appendix":[165,183],"keep":[171],"within":[176],"Thus,":[179],"eukaryote-specific":[181],"appears":[186],"stabilize":[188],"association":[190,207],"other":[192],"components":[193],"whereas":[198],"C-terminal":[200]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}