{"id":"https://openalex.org/W2154063141","doi":"https://doi.org/10.1081/jlc-120021255","title":"Spiral Disk Assembly for HSCCC: Column Design and Basic Studies on Chromatographic Resolution and Stationary Phase Retention","display_name":"Spiral Disk Assembly for HSCCC: Column Design and Basic Studies on Chromatographic Resolution and Stationary Phase Retention","publication_year":2003,"publication_date":"2003-06-01","ids":{"openalex":"https://openalex.org/W2154063141","doi":"https://doi.org/10.1081/jlc-120021255","mag":"2154063141"},"language":"en","primary_location":{"id":"doi:10.1081/jlc-120021255","doi":"https://doi.org/10.1081/jlc-120021255","is_oa":false,"landing_page_url":"https://doi.org/10.1081/jlc-120021255","pdf_url":null,"source":{"id":"https://openalex.org/S123147032","display_name":"Journal of Liquid Chromatography & Related Technologies","issn_l":"1082-6076","issn":["1082-6076","1520-572X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-d","doyens","jufo-1","norway-1","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320547","host_organization_name":"Taylor & Francis","host_organization_lineage":["https://openalex.org/P4310320547","https://openalex.org/P4310320449"],"host_organization_lineage_names":["Taylor & Francis","Informa"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Liquid Chromatography & Related Technologies","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5029128268","display_name":"Yoichiro Ito","orcid":"https://orcid.org/0000-0001-9018-295X","observed_orcids":["https://orcid.org/0000-0001-9018-295X"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210106489","display_name":"National Heart, Lung, and Blood Institute","ror":"https://ror.org/012pb6c26","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210106489"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Yoichiro Ito","raw_affiliation_strings":["a   Laboratory of Biophysical Chemistry, National Heart, Lung, and Blood Institute , National Institutes of Health , Bldg. 50, Rm 3334,  Bethesda ,  Maryland ,  20892\u20108014 ,  USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"a   Laboratory of Biophysical Chemistry, National Heart, Lung, and Blood Institute , National Institutes of Health , Bldg. 50, Rm 3334,  Bethesda ,  Maryland ,  20892\u20108014 ,  USA","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210106489"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111872254","display_name":"Fuquan Yang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210106489","display_name":"National Heart, Lung, and Blood Institute","ror":"https://ror.org/012pb6c26","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210106489"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Fuquan Yang","raw_affiliation_strings":["a   Laboratory of Biophysical Chemistry, National Heart, Lung, and Blood Institute , National Institutes of Health , Bldg. 50, Rm 3334,  Bethesda ,  Maryland ,  20892\u20108014 ,  USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"a   Laboratory of Biophysical Chemistry, National Heart, Lung, and Blood Institute , National Institutes of Health , Bldg. 50, Rm 3334,  Bethesda ,  Maryland ,  20892\u20108014 ,  USA","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210106489"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5077311379","display_name":"Paul Fitze","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Paul E. Fitze","raw_affiliation_strings":["b   Biomedical Engineering and Instrumentation Program , National Institutes of Health ,  Bethesda ,  Maryland ,  USA","Biomedical Engineering and Instrumentation Program , National Institutes of Health , Bethesda , Maryland , USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"b   Biomedical Engineering and Instrumentation Program , National Institutes of Health ,  Bethesda ,  Maryland ,  USA","institution_ids":["https://openalex.org/I1299303238"]},{"raw_affiliation_string":"Biomedical Engineering and Instrumentation Program , National Institutes of Health , Bethesda , Maryland , USA","institution_ids":["https://openalex.org/I1299303238"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5020738323","display_name":"James V. Sullivan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"James V. Sullivan","raw_affiliation_strings":["b   Biomedical Engineering and Instrumentation Program , National Institutes of Health ,  Bethesda ,  Maryland ,  USA","Biomedical Engineering and Instrumentation Program , National Institutes of Health , Bethesda , Maryland , USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"b   Biomedical Engineering and Instrumentation Program , National Institutes of Health ,  Bethesda ,  Maryland ,  USA","institution_ids":["https://openalex.org/I1299303238"]},{"raw_affiliation_string":"Biomedical Engineering and Instrumentation Program , National Institutes of Health , Bethesda , Maryland , USA","institution_ids":["https://openalex.org/I1299303238"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5029128268"],"corresponding_institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210106489"],"apc_list":null,"apc_paid":null,"fwci":2.4794,"has_fulltext":false,"cited_by_count":44,"citation_normalized_percentile":{"value":0.8714705,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":97},"biblio":{"volume":"26","issue":"9-10","first_page":"1355","last_page":"1372"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T13128","display_name":"Chromatography in Natural Products","score":0.8359000086784363,"subfield":{"id":"https://openalex.org/subfields/1602","display_name":"Analytical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T13128","display_name":"Chromatography in Natural Products","score":0.8359000086784363,"subfield":{"id":"https://openalex.org/subfields/1602","display_name":"Analytical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10908","display_name":"Analytical Chemistry and Chromatography","score":0.15379999577999115,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12850","display_name":"Field-Flow Fractionation Techniques","score":0.001500000013038516,"subfield":{"id":"https://openalex.org/subfields/2206","display_name":"Computational Mechanics"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/high-speed-countercurrent-chromatography","display_name":"high-speed countercurrent chromatography","score":0.9179999828338623},{"id":"https://openalex.org/keywords/stationary-phase-retention","display_name":"stationary phase retention","score":0.8820000290870667},{"id":"https://openalex.org/keywords/chromatographic-resolution","display_name":"chromatographic resolution","score":0.7570000290870667},{"id":"https://openalex.org/keywords/spiral-pitch","display_name":"spiral pitch","score":0.8029999732971191},{"id":"https://openalex.org/keywords/protein-separation","display_name":"protein separation","score":0.9800000190734863},{"id":"https://openalex.org/keywords/polyethylene-glycol","display_name":"polyethylene glycol","score":0.7770000100135803},{"id":"https://openalex.org/keywords/dextran","display_name":"dextran","score":0.9120000004768372}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.824122428894043},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.7263296842575073},{"id":"https://openalex.org/C63258622","wikidata":"https://www.wikidata.org/wiki/Q1498317","display_name":"Countercurrent chromatography","level":3,"score":0.7034518122673035},{"id":"https://openalex.org/C44280652","wikidata":"https://www.wikidata.org/wiki/Q104837","display_name":"Phase (matter)","level":2,"score":0.5461453199386597},{"id":"https://openalex.org/C174128100","wikidata":"https://www.wikidata.org/wiki/Q846907","display_name":"Spiral (railway)","level":2,"score":0.471692830324173},{"id":"https://openalex.org/C172120300","wikidata":"https://www.wikidata.org/wiki/Q1134348","display_name":"Volumetric flow rate","level":2,"score":0.4476739168167114},{"id":"https://openalex.org/C113196181","wikidata":"https://www.wikidata.org/wiki/Q485223","display_name":"Analytical Chemistry (journal)","level":2,"score":0.39269864559173584},{"id":"https://openalex.org/C179998833","wikidata":"https://www.wikidata.org/wiki/Q381233","display_name":"High-performance liquid chromatography","level":2,"score":0.24351680278778076},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.06869813799858093},{"id":"https://openalex.org/C97355855","wikidata":"https://www.wikidata.org/wiki/Q11473","display_name":"Thermodynamics","level":1,"score":0.06657537817955017},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.0},{"id":"https://openalex.org/C134306372","wikidata":"https://www.wikidata.org/wiki/Q7754","display_name":"Mathematical analysis","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1081/jlc-120021255","doi":"https://doi.org/10.1081/jlc-120021255","is_oa":false,"landing_page_url":"https://doi.org/10.1081/jlc-120021255","pdf_url":null,"source":{"id":"https://openalex.org/S123147032","display_name":"Journal of Liquid Chromatography & Related Technologies","issn_l":"1082-6076","issn":["1082-6076","1520-572X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-d","doyens","jufo-1","norway-1","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320547","host_organization_name":"Taylor & Francis","host_organization_lineage":["https://openalex.org/P4310320547","https://openalex.org/P4310320449"],"host_organization_lineage_names":["Taylor & Francis","Informa"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Liquid Chromatography & Related Technologies","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":7,"referenced_works":["https://openalex.org/W76437226","https://openalex.org/W1968615551","https://openalex.org/W2011038731","https://openalex.org/W2015133300","https://openalex.org/W2042006579","https://openalex.org/W2895340958","https://openalex.org/W4244455167"],"related_works":["https://openalex.org/W2031025111","https://openalex.org/W4230640406","https://openalex.org/W2059371634","https://openalex.org/W2123472525","https://openalex.org/W1976524662","https://openalex.org/W2168216791","https://openalex.org/W2007242515","https://openalex.org/W2050948371","https://openalex.org/W2380261801","https://openalex.org/W2012351175"],"abstract_inverted_index":{"A":[0],"set":[1],"of":[2,24,28,65,72,121,136,161,175,218,224,241,250],"four":[3,52],"separation":[4,199,254],"disks":[5,35,195,214],"equipped":[6],"with":[7,44,163,181,189],"spiral":[8,34,42,53,62,130,154,184,252],"channel(s)":[9],"was":[10,68,168],"designed":[11],"for":[12,90,103,119,139],"our":[13],"type\u2010J":[14],"high":[15,147],"speed":[16],"countercurrent":[17],"chromatography":[18],"(J\u2010HSCCC)":[19],"centrifuge":[20],"to":[21,58],"improve":[22],"retention":[23,78,217,223],"the":[25,48,129,153,164,176,190,193,197,201,212,219,225,231,248,251],"stationary":[26,76,122,137,220],"phase":[27,77,123,138,149,178,203],"polar":[29],"solvent":[30,83,141,166],"systems.":[31],"Four":[32],"different":[33,45,81],"were":[36],"tested:":[37],"two":[38,50],"had":[39,51],"a":[40,60,133,145,157,238],"single":[41],"channel":[43],"depths":[46],"and":[47,106,179],"other":[49],"channels":[54],"connected":[55],"in":[56,70,113,204,230],"series":[57],"provide":[59],"greater":[61,183],"pitch.":[63,185],"Performance":[64],"each":[66],"disk":[67,253],"tested":[69],"terms":[71],"chromatographic":[73],"resolution":[74],"and/or":[75],"using":[79,170,200],"three":[80],"two\u2010phase":[82],"systems,":[84,142],"including":[85],"1\u2010butanol/acetic":[86],"acid/water":[87],"(4:1:5,":[88],"v/v/v)":[89],"dipeptide":[91],"separation;":[92,105],"12.5%":[93],"(w/w)":[94,99,108,110],"polyethylene":[95],"glycol":[96],"(PEG)":[97],"1000\u201312.5%":[98],"dibasic":[100,116],"potassium":[101],"phosphate":[102,118],"protein":[104,187],"4%":[107],"PEG8000\u20135%":[109],"dextran":[111],"T500":[112],"10":[114,171],"mM":[115],"sodium":[117],"determination":[120],"retention.":[124],"The":[125,222],"results":[126],"show":[127,215],"that":[128],"column":[131,255],"retains":[132],"satisfactory":[134],"amount":[135],"all":[140],"even":[143],"at":[144,208,237],"relatively":[146],"mobile":[148,177],"flow":[150,173,239],"rate":[151,174,240],"where":[152],"pitch":[155],"plays":[156],"significant":[158],"role.":[159],"Separation":[160],"dipeptides":[162],"butanol":[165],"system":[167,227],"possible":[169],"mL/min":[172],"4\u2010spiral\u2010disks":[180],"their":[182],"In":[186],"separations":[188],"PEG\u2013phosphate":[191],"system,":[192],"single\u2010spiral":[194],"yielded":[196],"best":[198],"upper":[202],"tail\u2010to\u2010head":[205],"elution":[206],"mode":[207],"1":[209],"mL/min,":[210],"while":[211],"4\u2010spiral":[213,232],"higher":[216],"phase.":[221],"PEG\u2013dextran":[226],"is":[228],"improved":[229],"disks,":[233],"which":[234,246],"exceed":[235],"60%":[236],"0.5":[242],"mL/min.":[243],"Various":[244],"parameters,":[245],"affect":[247],"performance":[249],"are":[256],"discussed.":[257]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":2},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":3},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":2}],"updated_date":"2026-08-27T14:10:00.468798","created_date":"2016-06-24T00:00:00"}