{"id":"https://openalex.org/W2152193238","doi":"https://doi.org/10.1073/pnas.2036282100","title":"Comprehensive transposon mutant library of Pseudomonas aeruginosa","display_name":"Comprehensive transposon mutant library of Pseudomonas aeruginosa","publication_year":2003,"publication_date":"2003-11-14","ids":{"openalex":"https://openalex.org/W2152193238","doi":"https://doi.org/10.1073/pnas.2036282100","mag":"2152193238","pmid":"https://pubmed.ncbi.nlm.nih.gov/14617778","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/283593"},"language":"en","primary_location":{"id":"doi:10.1073/pnas.2036282100","doi":"https://doi.org/10.1073/pnas.2036282100","is_oa":false,"landing_page_url":"https://doi.org/10.1073/pnas.2036282100","pdf_url":null,"source":{"id":"https://openalex.org/S125754415","display_name":"Proceedings of the National Academy of Sciences","issn_l":"0027-8424","issn":["0027-8424","1091-6490"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320052","host_organization_name":"National Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310320052"],"host_organization_lineage_names":["National Academy of Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://europepmc.org/pmc/articles/PMC283593","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Michael A. Jacobs","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Michael A. Jacobs","raw_affiliation_strings":["Department of Medicine, University of Washington Genome Center, Box 352145, Seattle, WA 98195-2145; and Departments of Genome Sciences and Statistics, University of Washington, Seattle, WA 98195"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, University of Washington Genome Center, Box 352145, Seattle, WA 98195-2145; and Departments of Genome Sciences and Statistics, University of Washington, Seattle, WA 98195","institution_ids":["https://openalex.org/I201448701"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5054358294","display_name":"Ashley Alwood","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ashley Alwood","raw_affiliation_strings":["Department of Medicine, University of Washington Genome Center, Box 352145, Seattle, WA 98195-2145; and Departments of Genome Sciences and Statistics, University of Washington, Seattle, WA 98195"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, University of Washington Genome Center, Box 352145, Seattle, WA 98195-2145; and Departments of Genome Sciences and Statistics, University of Washington, Seattle, WA 98195","institution_ids":["https://openalex.org/I201448701"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5015818483","display_name":"Iyarit Thaipisuttikul","orcid":"https://orcid.org/0009-0007-0242-0171","observed_orcids":["https://orcid.org/0009-0007-0242-0171"]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Iyarit Thaipisuttikul","raw_affiliation_strings":["Department of Medicine, University of Washington Genome Center, Box 352145, Seattle, WA 98195-2145; and Departments of Genome Sciences and Statistics, University of Washington, Seattle, WA 98195"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, University of Washington Genome Center, Box 352145, Seattle, WA 98195-2145; and Departments of Genome Sciences and Statistics, University of Washington, Seattle, WA 98195","institution_ids":["https://openalex.org/I201448701"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5062422889","display_name":"David H. Spencer","orcid":"https://orcid.org/0000-0002-5314-3043","observed_orcids":["https://orcid.org/0000-0002-5314-3043"]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"David Spencer","raw_affiliation_strings":["Department of Medicine, University of Washington Genome Center, Box 352145, Seattle, WA 98195-2145; and Departments of Genome Sciences and Statistics, University of Washington, Seattle, WA 98195"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, University of Washington Genome Center, Box 352145, Seattle, WA 98195-2145; and Departments of Genome Sciences and Statistics, University of Washington, Seattle, WA 98195","institution_ids":["https://openalex.org/I201448701"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5026268911","display_name":"Eric Haugen","orcid":"https://orcid.org/0000-0001-7444-8981","observed_orcids":["https://orcid.org/0000-0001-7444-8981"]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Eric Haugen","raw_affiliation_strings":["Department of Medicine, University of Washington Genome Center, Box 352145, Seattle, WA 98195-2145; and Departments of Genome Sciences and Statistics, University of Washington, Seattle, WA 98195"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, University of Washington Genome Center, Box 352145, Seattle, WA 98195-2145; and Departments of Genome Sciences and Statistics, University of Washington, Seattle, WA 98195","institution_ids":["https://openalex.org/I201448701"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Stephen Ernst","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Stephen Ernst","raw_affiliation_strings":["Department of Medicine, University of Washington Genome Center, Box 352145, Seattle, WA 98195-2145; and Departments of Genome Sciences and Statistics, University of Washington, Seattle, WA 98195"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, University of Washington Genome Center, Box 352145, Seattle, WA 98195-2145; and Departments of Genome Sciences and Statistics, University of Washington, Seattle, WA 98195","institution_ids":["https://openalex.org/I201448701"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5063931343","display_name":"Oliver Will","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Oliver Will","raw_affiliation_strings":["Department of Medicine, University of Washington Genome Center, Box 352145, Seattle, WA 98195-2145; and Departments of Genome Sciences and Statistics, University of Washington, Seattle, WA 98195"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, University of Washington Genome Center, Box 352145, Seattle, WA 98195-2145; and Departments of Genome Sciences and Statistics, University of Washington, Seattle, WA 98195","institution_ids":["https://openalex.org/I201448701"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5015455225","display_name":"Rajinder Kaul","orcid":"https://orcid.org/0000-0002-7895-9284","observed_orcids":["https://orcid.org/0000-0002-7895-9284"]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Rajinder Kaul","raw_affiliation_strings":["Department of Medicine, University of Washington Genome Center, Box 352145, Seattle, WA 98195-2145; and Departments of Genome Sciences and Statistics, University of Washington, Seattle, WA 98195"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, University of Washington Genome Center, Box 352145, Seattle, WA 98195-2145; and Departments of Genome Sciences and Statistics, University of Washington, Seattle, WA 98195","institution_ids":["https://openalex.org/I201448701"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5102993893","display_name":"Christopher K. Raymond","orcid":"https://orcid.org/0000-0002-1417-4568","observed_orcids":["https://orcid.org/0000-0002-1417-4568","https://orcid.org/0000-0002-4072-0550"]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Christopher Raymond","raw_affiliation_strings":["Department of Medicine, University of Washington Genome Center, Box 352145, Seattle, WA 98195-2145; and Departments of Genome Sciences and Statistics, University of Washington, Seattle, WA 98195"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, University of Washington Genome Center, Box 352145, Seattle, WA 98195-2145; and Departments of Genome Sciences and Statistics, University of Washington, Seattle, WA 98195","institution_ids":["https://openalex.org/I201448701"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5015209576","display_name":"Ruth Levy","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ruth Levy","raw_affiliation_strings":["Department of Medicine, University of Washington Genome Center, Box 352145, Seattle, WA 98195-2145; and Departments of Genome Sciences and Statistics, University of Washington, Seattle, WA 98195"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, University of Washington Genome Center, Box 352145, Seattle, WA 98195-2145; and Departments of Genome Sciences and Statistics, University of Washington, Seattle, WA 98195","institution_ids":["https://openalex.org/I201448701"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5001789545","display_name":"Chunrong Liu","orcid":"https://orcid.org/0000-0002-9240-0380","observed_orcids":["https://orcid.org/0000-0002-9240-0380"]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Liu Chun-Rong","raw_affiliation_strings":["Department of Medicine, University of Washington Genome Center, Box 352145, Seattle, WA 98195-2145; and Departments of Genome Sciences and Statistics, University of Washington, Seattle, WA 98195"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, University of Washington Genome Center, Box 352145, Seattle, WA 98195-2145; and Departments of Genome Sciences and Statistics, University of Washington, Seattle, WA 98195","institution_ids":["https://openalex.org/I201448701"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5071622154","display_name":"Donald Guenthner","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Donald Guenthner","raw_affiliation_strings":["Department of Medicine, University of Washington Genome Center, Box 352145, Seattle, WA 98195-2145; and Departments of Genome Sciences and Statistics, University of Washington, Seattle, WA 98195"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, University of Washington Genome Center, Box 352145, Seattle, WA 98195-2145; and Departments of Genome Sciences and Statistics, University of Washington, Seattle, WA 98195","institution_ids":["https://openalex.org/I201448701"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5083686348","display_name":"Donald Bovee","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Donald Bovee","raw_affiliation_strings":["Department of Medicine, University of Washington Genome Center, Box 352145, Seattle, WA 98195-2145; and Departments of Genome Sciences and Statistics, University of Washington, Seattle, WA 98195"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, University of Washington Genome Center, Box 352145, Seattle, WA 98195-2145; and Departments of Genome Sciences and Statistics, University of Washington, Seattle, WA 98195","institution_ids":["https://openalex.org/I201448701"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5105759334","display_name":"Maynard V. Olson","orcid":"https://orcid.org/0000-0003-3527-9497","observed_orcids":["https://orcid.org/0000-0003-3527-9497"]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Maynard V. Olson","raw_affiliation_strings":["Department of Medicine, University of Washington Genome Center, Box 352145, Seattle, WA 98195-2145; and Departments of Genome Sciences and Statistics, University of Washington, Seattle, WA 98195"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, University of Washington Genome Center, Box 352145, Seattle, WA 98195-2145; and Departments of Genome Sciences and Statistics, University of Washington, Seattle, WA 98195","institution_ids":["https://openalex.org/I201448701"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5014003771","display_name":"Colin Manoil","orcid":"https://orcid.org/0000-0001-6773-3434","observed_orcids":["https://orcid.org/0000-0001-6773-3434"]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Colin Manoil","raw_affiliation_strings":["Department of Medicine, University of Washington Genome Center, Box 352145, Seattle, WA 98195-2145; and Departments of Genome Sciences and Statistics, University of Washington, Seattle, WA 98195"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, University of Washington Genome Center, Box 352145, Seattle, WA 98195-2145; and Departments of Genome Sciences and Statistics, University of Washington, Seattle, WA 98195","institution_ids":["https://openalex.org/I201448701"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I201448701"],"apc_list":null,"apc_paid":null,"fwci":13.7346,"has_fulltext":false,"cited_by_count":1108,"citation_normalized_percentile":{"value":0.99439513,"is_in_top_1_percent":true,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":99,"max":100},"biblio":{"volume":"100","issue":"24","first_page":"14339","last_page":"14344"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.9907000064849854,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.9907000064849854,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.0015999999595806003,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.0012000000569969416,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/pseudomonas-aeruginosa","display_name":"Pseudomonas aeruginosa","score":0.9229999780654907},{"id":"https://openalex.org/keywords/transposon-mutant-library","display_name":"transposon mutant library","score":0.8980000019073486},{"id":"https://openalex.org/keywords/essential-genes","display_name":"essential genes","score":0.8100000023841858},{"id":"https://openalex.org/keywords/non-essential-genes","display_name":"non-essential genes","score":0.8379999995231628},{"id":"https://openalex.org/keywords/twitching-motility","display_name":"twitching motility","score":0.843999981880188},{"id":"https://openalex.org/keywords/mutant-screening","display_name":"mutant screening","score":0.5410000085830688},{"id":"https://openalex.org/keywords/gene-essentiality","display_name":"gene essentiality","score":0.39800000190734863},{"id":"https://openalex.org/keywords/opportunistic-pathogens","display_name":"opportunistic pathogens","score":0.7080000042915344}],"concepts":[{"id":"https://openalex.org/C4918238","wikidata":"https://www.wikidata.org/wiki/Q121438","display_name":"Transposable element","level":4,"score":0.8593679666519165},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7959126234054565},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.7284711003303528},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.7208795547485352},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.7124885320663452},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.5401901006698608},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.4037700295448303}],"mesh":[{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D005798","descriptor_name":"Genes, Bacterial","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D010641","descriptor_name":"Phenotype","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011550","descriptor_name":"Pseudomonas aeruginosa","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D013045","descriptor_name":"Species Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015723","descriptor_name":"Gene Library","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D016254","descriptor_name":"Mutagenesis, Insertional","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1073/pnas.2036282100","doi":"https://doi.org/10.1073/pnas.2036282100","is_oa":false,"landing_page_url":"https://doi.org/10.1073/pnas.2036282100","pdf_url":null,"source":{"id":"https://openalex.org/S125754415","display_name":"Proceedings of the National Academy of Sciences","issn_l":"0027-8424","issn":["0027-8424","1091-6490"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320052","host_organization_name":"National Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310320052"],"host_organization_lineage_names":["National Academy of Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:279895","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC283593","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:283593","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/283593","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proc Natl Acad Sci U S A","raw_type":"Text"},{"id":"pmid:14617778","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/14617778","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences of the United States of America","raw_type":"Comparative Study"}],"best_oa_location":{"id":"pmh:oai:europepmc.org:279895","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC283593","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":16,"referenced_works":["https://openalex.org/W1487722613","https://openalex.org/W1512047334","https://openalex.org/W1658092509","https://openalex.org/W1974299093","https://openalex.org/W1991132315","https://openalex.org/W2072522551","https://openalex.org/W2084832691","https://openalex.org/W2119865469","https://openalex.org/W2142437744","https://openalex.org/W2142648753","https://openalex.org/W2149353640","https://openalex.org/W2152298121","https://openalex.org/W2164997719","https://openalex.org/W2332078328","https://openalex.org/W2797066131","https://openalex.org/W7099585975"],"related_works":["https://openalex.org/W2118915737","https://openalex.org/W2133425351","https://openalex.org/W3210501342","https://openalex.org/W2287203008","https://openalex.org/W2410853724","https://openalex.org/W2068651456","https://openalex.org/W616284349","https://openalex.org/W189384730","https://openalex.org/W1987486474","https://openalex.org/W7098445381"],"abstract_inverted_index":{"We":[0],"have":[1,181],"developed":[2],"technologies":[3],"for":[4,34,37,98,131,169],"creating":[5],"saturating":[6],"libraries":[7,23],"of":[8,21,30,65,73,80,84,90,123,158,166,189],"sequence-defined":[9,67],"transposon":[10],"insertion":[11],"mutants":[12,145],"in":[13,114,134],"which":[14,38],"each":[15],"strain":[16],"is":[17,126],"maintained.":[18],"Phenotypic":[19],"analysis":[20,157],"such":[22],"should":[24,185],"provide":[25],"a":[26,39,56],"virtually":[27],"complete":[28,164],"identification":[29],"nonessential":[31],"genes":[32,83,92,113,125,150,167],"required":[33,168],"any":[35],"process":[36],"suitable":[40],"screen":[41],"can":[42],"be":[43,96],"devised.":[44],"The":[45,59,173],"approach":[46],"was":[47,62],"applied":[48],"to":[49,70,95,110,147,176],"Pseudomonas":[50],"aeruginosa,":[51],"an":[52,71],"opportunistic":[53],"pathogen":[54],"with":[55],"6.3-Mbp":[57],"genome.":[58],"library":[60],"that":[61,119,184],"generated":[63],"consists":[64],"30,100":[66],"mutants,":[68],"corresponding":[69,146],"average":[72],"five":[74],"insertions":[75],"per":[76],"gene.":[77],"About":[78],"12%":[79],"the":[81,120,129,159,178],"predicted":[82],"this":[85],"organism":[86],"lacked":[87],"insertions;":[88],"many":[89],"these":[91],"are":[93],"likely":[94],"essential":[97,112,124],"growth":[99],"on":[100,104],"rich":[101],"media.":[102],"Based":[103],"statistical":[105],"analyses":[106],"and":[107,141,195],"bioinformatic":[108],"comparison":[109],"known":[111],"E.":[115],"coli,":[116],"we":[117],"estimate":[118],"actual":[121],"number":[122],"300-400.":[127],"Screening":[128],"collection":[130,160,180],"strains":[132],"defective":[133],"two":[135],"defined":[136],"multigenic":[137],"processes":[138],"(twitching":[139],"motility":[140],"prototrophic":[142],"growth)":[143],"identified":[144],"nearly":[148],"all":[149],"expected":[151],"from":[152],"earlier":[153],"studies.":[154],"Thus,":[155],"phenotypic":[156],"may":[161],"produce":[162],"essentially":[163],"lists":[165],"diverse":[170],"biological":[171],"activities.":[172],"transposons":[174],"used":[175],"generate":[177],"mutant":[179],"added":[182],"features":[183],"facilitate":[186],"downstream":[187],"studies":[188],"gene":[190],"expression,":[191],"protein":[192],"localization,":[193],"epistasis,":[194],"chromosome":[196],"engineering.":[197]},"counts_by_year":[{"year":2026,"cited_by_count":28},{"year":2025,"cited_by_count":43},{"year":2024,"cited_by_count":43},{"year":2023,"cited_by_count":46},{"year":2022,"cited_by_count":45},{"year":2021,"cited_by_count":44},{"year":2020,"cited_by_count":51},{"year":2019,"cited_by_count":62},{"year":2018,"cited_by_count":58},{"year":2017,"cited_by_count":50},{"year":2016,"cited_by_count":59},{"year":2015,"cited_by_count":65},{"year":2014,"cited_by_count":65},{"year":2013,"cited_by_count":60},{"year":2012,"cited_by_count":44}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}
