{"id":"https://openalex.org/W2151153071","doi":"https://doi.org/10.1111/j.1469-8137.2010.03282.x","title":"Reactive oxygen species\u2010induced release of intracellular ascorbate in plant cell\u2010suspension cultures and evidence for pulsing of net release rate","display_name":"Reactive oxygen species\u2010induced release of intracellular ascorbate in plant cell\u2010suspension cultures and evidence for pulsing of net release rate","publication_year":2010,"publication_date":"2010-05-10","ids":{"openalex":"https://openalex.org/W2151153071","doi":"https://doi.org/10.1111/j.1469-8137.2010.03282.x","mag":"2151153071","pmid":"https://pubmed.ncbi.nlm.nih.gov/20487313"},"language":"en","primary_location":{"id":"doi:10.1111/j.1469-8137.2010.03282.x","doi":"https://doi.org/10.1111/j.1469-8137.2010.03282.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1469-8137.2010.03282.x","pdf_url":null,"source":{"id":"https://openalex.org/S58631098","display_name":"New Phytologist","issn_l":"0028-646X","issn":["0028-646X","1469-8137"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","jufo-3","ki-jl-1","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"New Phytologist","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5005903201","display_name":"Harriet T. Parsons","orcid":"https://orcid.org/0000-0003-1666-9123","observed_orcids":["https://orcid.org/0000-0003-1666-9123"]},"institutions":[{"id":"https://openalex.org/I98677209","display_name":"University of Edinburgh","ror":"https://ror.org/01nrxwf90","country_code":"GB","type":"education","lineage":["https://openalex.org/I98677209"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Harriet T. Parsons","raw_affiliation_strings":["The Edinburgh Cell Wall Group, Institute of Molecular Plant Sciences, School of Biological Sciences, The University of Edinburgh, The King's Buildings, Mayfield Road, Edinburgh EH9 3JH, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Edinburgh Cell Wall Group, Institute of Molecular Plant Sciences, School of Biological Sciences, The University of Edinburgh, The King's Buildings, Mayfield Road, Edinburgh EH9 3JH, UK","institution_ids":["https://openalex.org/I98677209"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5024224974","display_name":"Stephen C. Fry","orcid":"https://orcid.org/0000-0002-1820-4867","observed_orcids":["https://orcid.org/0000-0002-1820-4867"]},"institutions":[{"id":"https://openalex.org/I98677209","display_name":"University of Edinburgh","ror":"https://ror.org/01nrxwf90","country_code":"GB","type":"education","lineage":["https://openalex.org/I98677209"]}],"countries":["GB"],"is_corresponding":true,"raw_author_name":"Stephen C. Fry","raw_affiliation_strings":["The Edinburgh Cell Wall Group, Institute of Molecular Plant Sciences, School of Biological Sciences, The University of Edinburgh, The King's Buildings, Mayfield Road, Edinburgh EH9 3JH, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Edinburgh Cell Wall Group, Institute of Molecular Plant Sciences, School of Biological Sciences, The University of Edinburgh, The King's Buildings, Mayfield Road, Edinburgh EH9 3JH, UK","institution_ids":["https://openalex.org/I98677209"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5024224974"],"corresponding_institution_ids":["https://openalex.org/I98677209"],"apc_list":{"value":5790,"currency":"USD","value_usd":5790},"apc_paid":null,"fwci":4.5449,"has_fulltext":false,"cited_by_count":32,"citation_normalized_percentile":{"value":0.94253765,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":87,"max":99},"biblio":{"volume":"187","issue":"2","first_page":"332","last_page":"342"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10014","display_name":"Plant Stress Responses and Tolerance","score":0.9878000020980835,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10014","display_name":"Plant Stress Responses and Tolerance","score":0.9878000020980835,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11884","display_name":"Redox biology and oxidative stress","score":0.0071000000461936,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12554","display_name":"Vitamin C and Antioxidants Research","score":0.0010999999940395355,"subfield":{"id":"https://openalex.org/subfields/2916","display_name":"Nutrition and Dietetics"},"field":{"id":"https://openalex.org/fields/29","display_name":"Nursing"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/reactive-oxygen-species","display_name":"reactive oxygen species","score":0.9810000061988831},{"id":"https://openalex.org/keywords/hydrogen-peroxide","display_name":"hydrogen peroxide","score":0.9679999947547913},{"id":"https://openalex.org/keywords/apoplast","display_name":"apoplast","score":0.5910000205039978},{"id":"https://openalex.org/keywords/oxidative-stress","display_name":"oxidative stress","score":0.7559999823570251},{"id":"https://openalex.org/keywords/dehydroascorbic-acid","display_name":"dehydroascorbic acid","score":0.9309999942779541},{"id":"https://openalex.org/keywords/plant-cell-suspension-culture","display_name":"plant cell suspension culture","score":0.6890000104904175},{"id":"https://openalex.org/keywords/roses","display_name":"roses","score":0.7229999899864197},{"id":"https://openalex.org/keywords/arabidopsis","display_name":"Arabidopsis","score":0.9819999933242798},{"id":"https://openalex.org/keywords/antioxidant-defense","display_name":"antioxidant defense","score":0.5360000133514404},{"id":"https://openalex.org/keywords/cell-growth-phase","display_name":"cell growth phase","score":0.5}],"concepts":[{"id":"https://openalex.org/C2993787908","wikidata":"https://www.wikidata.org/wiki/Q189111","display_name":"Suspension culture","level":3,"score":0.6830867528915405},{"id":"https://openalex.org/C48349386","wikidata":"https://www.wikidata.org/wiki/Q424361","display_name":"Reactive oxygen species","level":2,"score":0.6128467321395874},{"id":"https://openalex.org/C79879829","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Intracellular","level":2,"score":0.6052835583686829},{"id":"https://openalex.org/C105341887","wikidata":"https://www.wikidata.org/wiki/Q1307987","display_name":"Suspension (topology)","level":3,"score":0.5999674797058105},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.5662872195243835},{"id":"https://openalex.org/C540031477","wikidata":"https://www.wikidata.org/wiki/Q629","display_name":"Oxygen","level":2,"score":0.5006892681121826},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.49779224395751953},{"id":"https://openalex.org/C156203801","wikidata":"https://www.wikidata.org/wiki/Q1056194","display_name":"Plant cell","level":3,"score":0.4245818257331848},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.35845351219177246},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.35517531633377075},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.3305218517780304},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3100791573524475},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3077910542488098},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.0},{"id":"https://openalex.org/C5961521","wikidata":"https://www.wikidata.org/wiki/Q746083","display_name":"Homotopy","level":2,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C202444582","wikidata":"https://www.wikidata.org/wiki/Q837863","display_name":"Pure mathematics","level":1,"score":0.0},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D001205","descriptor_name":"Ascorbic Acid","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D002250","descriptor_name":"Carbon Radioisotopes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005632","descriptor_name":"Fructose","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D006861","descriptor_name":"Hydrogen Peroxide","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D007477","descriptor_name":"Ions","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011851","descriptor_name":"Radioactivity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017360","descriptor_name":"Arabidopsis","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D017360","descriptor_name":"Arabidopsis","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D017360","descriptor_name":"Arabidopsis","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D017382","descriptor_name":"Reactive Oxygen Species","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D017382","descriptor_name":"Reactive Oxygen Species","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D018929","descriptor_name":"Cell Culture Techniques","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D027826","descriptor_name":"Rosa","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D027826","descriptor_name":"Rosa","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D027826","descriptor_name":"Rosa","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D042541","descriptor_name":"Intracellular Space","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D042541","descriptor_name":"Intracellular Space","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1111/j.1469-8137.2010.03282.x","doi":"https://doi.org/10.1111/j.1469-8137.2010.03282.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1469-8137.2010.03282.x","pdf_url":null,"source":{"id":"https://openalex.org/S58631098","display_name":"New Phytologist","issn_l":"0028-646X","issn":["0028-646X","1469-8137"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","jufo-3","ki-jl-1","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"New Phytologist","raw_type":"journal-article"},{"id":"pmid:20487313","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/20487313","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The New phytologist","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2895618403","display_name":null,"funder_award_id":"D19626","funder_id":"https://openalex.org/F4320334629","funder_display_name":"Biotechnology and Biological Sciences Research Council"}],"funders":[{"id":"https://openalex.org/F4320334629","display_name":"Biotechnology and Biological Sciences Research Council","ror":"https://ror.org/00cwqg982"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":54,"referenced_works":["https://openalex.org/W2690919","https://openalex.org/W575083446","https://openalex.org/W1586181119","https://openalex.org/W1595832217","https://openalex.org/W1764608545","https://openalex.org/W1805879128","https://openalex.org/W1821299455","https://openalex.org/W1966993429","https://openalex.org/W1969700447","https://openalex.org/W1974748526","https://openalex.org/W1981999048","https://openalex.org/W1987377358","https://openalex.org/W1988061962","https://openalex.org/W1988244994","https://openalex.org/W1991769123","https://openalex.org/W1995335307","https://openalex.org/W2000160067","https://openalex.org/W2002218466","https://openalex.org/W2002924564","https://openalex.org/W2016715662","https://openalex.org/W2023922687","https://openalex.org/W2030060617","https://openalex.org/W2036192690","https://openalex.org/W2037446180","https://openalex.org/W2040709631","https://openalex.org/W2048069216","https://openalex.org/W2049782573","https://openalex.org/W2058876576","https://openalex.org/W2059730796","https://openalex.org/W2062864424","https://openalex.org/W2072976273","https://openalex.org/W2095081648","https://openalex.org/W2100490287","https://openalex.org/W2105693282","https://openalex.org/W2105882695","https://openalex.org/W2109503678","https://openalex.org/W2109809268","https://openalex.org/W2111908322","https://openalex.org/W2112279687","https://openalex.org/W2115538542","https://openalex.org/W2119486813","https://openalex.org/W2128707341","https://openalex.org/W2133351712","https://openalex.org/W2140199266","https://openalex.org/W2144620357","https://openalex.org/W2149710171","https://openalex.org/W2150539008","https://openalex.org/W2150563446","https://openalex.org/W2152835788","https://openalex.org/W2157528000","https://openalex.org/W2161251566","https://openalex.org/W2167732960","https://openalex.org/W2168940882","https://openalex.org/W2337574226"],"related_works":["https://openalex.org/W1592961391","https://openalex.org/W2591913876","https://openalex.org/W2793931482","https://openalex.org/W2736294409","https://openalex.org/W2098232181","https://openalex.org/W4394591544","https://openalex.org/W2111293030","https://openalex.org/W2051531078","https://openalex.org/W2048069216","https://openalex.org/W7096471824"],"abstract_inverted_index":{"SUMMARY:":[0],"*Apoplastic":[1],"ascorbate":[2,154,175,193],"has":[3],"been":[4],"proposed":[5],"to":[6,9,111,115,147,187],"confer":[7],"resistance":[8],"oxidative":[10,188],"stresses,":[11],"e.g.":[12],"ozone.":[13],"We":[14],"investigated":[15],"reactive":[16],"oxygen":[17],"species":[18],"(ROS)-induced":[19],"secretion":[20,39],"and":[21,30,38,58,76,117,131,144],"catabolism":[22],"of":[23,28,74,81,107,124,134],"ascorbate.":[24,148],"*Late-growth-phase":[25],"cultured":[26,181],"cells":[27,143,164,172,183],"rose":[29],"Arabidopsis":[31],"were":[32,41],"preloaded":[33],"with":[34],"[(14)C]ascorbate.":[35],"Radiolabelled":[36],"metabolites":[37],"products":[40],"analysed":[42],"by":[43,141,190],"high-voltage":[44],"electrophoresis.":[45],"*In":[46],"both":[47],"species,":[48],"exogenous":[49],"1":[50],"mM":[51],"hydrogen":[52],"peroxide":[53],"(H(2)O(2))":[54],"rapidly":[55],"stimulated":[56],"[(14)C]ascorbate":[57,110,130],"[(14)C]dehydroascorbate":[59],"accumulation":[60],"in":[61,161,170],"the":[62,101,108,121,125,135,142,195],"medium":[63],"(apoplast).":[64],"Net":[65],"(14)C":[66],"export":[67],"was":[68,87,138],"most":[69,173],"rapid":[70],"within":[71],"100":[72],"s":[73],"washing,":[75],"often":[77],"showed":[78],"superimposed":[79],"pulses,":[80],"c.":[82,105],"10-s":[83],"duration,":[84],"whose":[85],"amplitude":[86],"greater":[88],"after":[89],"H(2)O(2)":[90,103],"treatment.":[91],"Oxidative":[92],"stress":[93,189],"did":[94],"not":[95],"cause":[96],"indiscriminate":[97],"metabolite":[98],"leakage":[99],"from":[100],"cells.":[102],"caused":[104],"20-40%":[106],"intracellular":[109],"be":[112],"irreversibly":[113,167,177],"catabolized":[114],"[(14)C]oxalyl-threonate":[116],"related":[118],"products;":[119],"however,":[120],"great":[122],"majority":[123],"extracellular":[126,174],"radioactivity":[127],"remained":[128],"as":[129],"[(14)C]dehydroascorbate.":[132],"Much":[133],"apoplastic":[136,158],"dehydroascorbate":[137],"probably":[139],"reabsorbed":[140],"reduced":[145],"back":[146],"*The":[149],"data":[150],"show":[151],"that":[152],"exported":[153],"can":[155,184],"serve":[156],"an":[157],"antioxidant":[159],"role":[160],"these":[162],"late-growth-phase":[163],"without":[165],"being":[166],"lost,":[168],"whereas":[169],"early-growth-phase":[171],"is":[176],"degraded.":[178],"In":[179],"conclusion,":[180],"plant":[182],"respond":[185],"actively":[186],"reversibly":[191],"exporting":[192],"into":[194],"apoplast.":[196]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":5},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":6},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":2},{"year":2012,"cited_by_count":7}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}