{"id":"https://openalex.org/W2151123937","doi":"https://doi.org/10.1002/jez.1402580313","title":"The meiotic response of cumulus cell\u2010enclosed mouse oocytes to follicle\u2010stimulating hormone in the presence of different macromolecules","display_name":"The meiotic response of cumulus cell\u2010enclosed mouse oocytes to follicle\u2010stimulating hormone in the presence of different macromolecules","publication_year":1991,"publication_date":"1991-06-01","ids":{"openalex":"https://openalex.org/W2151123937","doi":"https://doi.org/10.1002/jez.1402580313","mag":"2151123937","pmid":"https://pubmed.ncbi.nlm.nih.gov/1653817"},"language":"en","primary_location":{"id":"doi:10.1002/jez.1402580313","doi":"https://doi.org/10.1002/jez.1402580313","is_oa":false,"landing_page_url":"https://doi.org/10.1002/jez.1402580313","pdf_url":null,"source":{"id":"https://openalex.org/S4210222064","display_name":"Journal of Experimental Zoology","issn_l":"0022-104X","issn":["0022-104X","1097-010X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Experimental Zoology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Stephen M. Downs","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I102461120","display_name":"Marquette University","ror":"https://ror.org/04gr4te78","country_code":"US","type":"education","lineage":["https://openalex.org/I102461120"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Stephen M. Downs","raw_affiliation_strings":["Biology Department, Marquette University, Milwaukee, Wisconsin 53233"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biology Department, Marquette University, Milwaukee, Wisconsin 53233","institution_ids":["https://openalex.org/I102461120"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111690388","display_name":"Mark P.D. Dow","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I102461120","display_name":"Marquette University","ror":"https://ror.org/04gr4te78","country_code":"US","type":"education","lineage":["https://openalex.org/I102461120"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Mark P. D. Dow","raw_affiliation_strings":["Biology Department, Marquette University, Milwaukee, Wisconsin 53233"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biology Department, Marquette University, Milwaukee, Wisconsin 53233","institution_ids":["https://openalex.org/I102461120"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5088159038","display_name":"C. Funsho Fagbohun","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I102461120","display_name":"Marquette University","ror":"https://ror.org/04gr4te78","country_code":"US","type":"education","lineage":["https://openalex.org/I102461120"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"C. Funsho Fagbohun","raw_affiliation_strings":["Biology Department, Marquette University, Milwaukee, Wisconsin 53233"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biology Department, Marquette University, Milwaukee, Wisconsin 53233","institution_ids":["https://openalex.org/I102461120"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I102461120"],"apc_list":null,"apc_paid":null,"fwci":0.8913,"has_fulltext":false,"cited_by_count":13,"citation_normalized_percentile":{"value":0.64915644,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":95},"biblio":{"volume":"258","issue":"3","first_page":"373","last_page":"383"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10364","display_name":"Reproductive Biology and Fertility","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10364","display_name":"Reproductive Biology and Fertility","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11505","display_name":"Reproductive biology and impacts on aquatic species","score":0.000699999975040555,"subfield":{"id":"https://openalex.org/subfields/1314","display_name":"Physiology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10584","display_name":"Reproductive Physiology in Livestock","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1103","display_name":"Animal Science and Zoology"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/oocyte-maturation","display_name":"oocyte maturation","score":0.8759999871253967},{"id":"https://openalex.org/keywords/follicle-stimulating-hormone","display_name":"follicle-stimulating hormone","score":0.9929999709129333},{"id":"https://openalex.org/keywords/germinal-vesicle-breakdown","display_name":"germinal vesicle breakdown","score":0.8479999899864197},{"id":"https://openalex.org/keywords/bovine-serum-albumin","display_name":"bovine serum albumin","score":0.8619999885559082},{"id":"https://openalex.org/keywords/fetal-bovine-serum","display_name":"fetal bovine serum","score":0.8109999895095825},{"id":"https://openalex.org/keywords/serum-inhibitory-factor","display_name":"serum inhibitory factor","score":0.7870000004768372},{"id":"https://openalex.org/keywords/polyvinylpyrrolidone","display_name":"polyvinylpyrrolidone","score":0.902999997138977},{"id":"https://openalex.org/keywords/polyvinyl-alcohol","display_name":"polyvinyl alcohol","score":0.8730000257492065},{"id":"https://openalex.org/keywords/mouse-oocytes","display_name":"mouse oocytes","score":0.5870000123977661},{"id":"https://openalex.org/keywords/meiotic-arrest","display_name":"meiotic arrest","score":0.5329999923706055}],"concepts":[{"id":"https://openalex.org/C86807702","wikidata":"https://www.wikidata.org/wiki/Q2620381","display_name":"Bovine serum albumin","level":2,"score":0.7130143642425537},{"id":"https://openalex.org/C2994119143","wikidata":"https://www.wikidata.org/wiki/Q3246226","display_name":"Germinal vesicle","level":4,"score":0.642870306968689},{"id":"https://openalex.org/C2776690073","wikidata":"https://www.wikidata.org/wiki/Q3246226","display_name":"Oocyte","level":3,"score":0.539016842842102},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.5170196294784546},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.5134909749031067},{"id":"https://openalex.org/C16685009","wikidata":"https://www.wikidata.org/wiki/Q335057","display_name":"Andrology","level":1,"score":0.49842023849487305},{"id":"https://openalex.org/C2779404806","wikidata":"https://www.wikidata.org/wiki/Q488614","display_name":"Fetal bovine serum","level":3,"score":0.4962993264198303},{"id":"https://openalex.org/C2779279165","wikidata":"https://www.wikidata.org/wiki/Q200774","display_name":"Follicle-stimulating hormone","level":4,"score":0.49357759952545166},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.47627466917037964},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.38007989525794983},{"id":"https://openalex.org/C71315377","wikidata":"https://www.wikidata.org/wiki/Q11364","display_name":"Hormone","level":2,"score":0.330222487449646},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.27008485794067383},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.182010680437088},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.17912885546684265},{"id":"https://openalex.org/C2778575703","wikidata":"https://www.wikidata.org/wiki/Q50265477","display_name":"Luteinizing hormone","level":3,"score":0.17676344513893127},{"id":"https://openalex.org/C196843134","wikidata":"https://www.wikidata.org/wiki/Q33196","display_name":"Embryo","level":2,"score":0.16212505102157593},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.0831819474697113}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001769","descriptor_name":"Blood","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003470","descriptor_name":"Culture Media","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D003994","descriptor_name":"Bucladesine","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005640","descriptor_name":"Follicle Stimulating Hormone","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D006080","descriptor_name":"Ovarian Follicle","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008540","descriptor_name":"Meiosis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008810","descriptor_name":"Mice, Inbred C57BL","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009865","descriptor_name":"Oocytes","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D009865","descriptor_name":"Oocytes","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D011108","descriptor_name":"Polymers","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D012710","descriptor_name":"Serum Albumin, Bovine","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D013223","descriptor_name":"Statistics as Topic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1002/jez.1402580313","doi":"https://doi.org/10.1002/jez.1402580313","is_oa":false,"landing_page_url":"https://doi.org/10.1002/jez.1402580313","pdf_url":null,"source":{"id":"https://openalex.org/S4210222064","display_name":"Journal of Experimental Zoology","issn_l":"0022-104X","issn":["0022-104X","1097-010X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Experimental Zoology","raw_type":"journal-article"},{"id":"pmid:1653817","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/1653817","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of experimental zoology","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G6920419832","display_name":"PURINES AND THE MAINTENANCE OF MEIOTIC ARREST","funder_award_id":"R01HD025291","funder_id":"https://openalex.org/F4320337611","funder_display_name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development"}],"funders":[{"id":"https://openalex.org/F4320337611","display_name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","ror":"https://ror.org/04byxyr05"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":49,"referenced_works":["https://openalex.org/W118502676","https://openalex.org/W120206495","https://openalex.org/W197172311","https://openalex.org/W1497776019","https://openalex.org/W1579899042","https://openalex.org/W1860262571","https://openalex.org/W1967936252","https://openalex.org/W1974265269","https://openalex.org/W1977764079","https://openalex.org/W1978068964","https://openalex.org/W1986140299","https://openalex.org/W1986204387","https://openalex.org/W1992100444","https://openalex.org/W1995548246","https://openalex.org/W1996369550","https://openalex.org/W2004058476","https://openalex.org/W2006323812","https://openalex.org/W2008803545","https://openalex.org/W2011348946","https://openalex.org/W2011663094","https://openalex.org/W2018653760","https://openalex.org/W2030016174","https://openalex.org/W2030771022","https://openalex.org/W2044027914","https://openalex.org/W2049717556","https://openalex.org/W2053299519","https://openalex.org/W2054167608","https://openalex.org/W2055270229","https://openalex.org/W2055731841","https://openalex.org/W2073656002","https://openalex.org/W2088831286","https://openalex.org/W2091132247","https://openalex.org/W2098693385","https://openalex.org/W2102126511","https://openalex.org/W2103750154","https://openalex.org/W2118383838","https://openalex.org/W2124918908","https://openalex.org/W2126564608","https://openalex.org/W2137260221","https://openalex.org/W2149435484","https://openalex.org/W2150083639","https://openalex.org/W2152599703","https://openalex.org/W2154737050","https://openalex.org/W2169310316","https://openalex.org/W2233206182","https://openalex.org/W2410961661","https://openalex.org/W2438650246","https://openalex.org/W2584091758","https://openalex.org/W2998870364"],"related_works":["https://openalex.org/W1974265269","https://openalex.org/W2359662017","https://openalex.org/W2074492710","https://openalex.org/W2081041798","https://openalex.org/W2046781755","https://openalex.org/W2043696894","https://openalex.org/W2074743475","https://openalex.org/W2013597575","https://openalex.org/W2012296521","https://openalex.org/W2165279065"],"abstract_inverted_index":{"Experiments":[0],"were":[1,26,102,188],"carried":[2],"out":[3],"to":[4,108,112,152,205,287],"determine":[5],"the":[6,12,40,53,122,261,264,270,273,284,290,301],"effect":[7,148,216],"of":[8,17,55,68,93,100,125,130,175,263,272,292,296,304],"different":[9],"macromolecules":[10],"on":[11,217,269],"follicle-stimulating":[13],"hormone":[14,302],"(FSH)-induced":[15],"maturation":[16,84,101,131,176,199,225,236,247],"mouse":[18],"oocytes":[19,24,308],"in":[20,33,85,127,167,177,226,237,250,309],"culture.":[21,310],"Cumulus":[22],"cell-enclosed":[23,307],"(CEO)":[25],"isolated":[27,305],"from":[28,182],"gonadotropin-primed":[29],"mice":[30],"and":[31,156,186,259],"maintained":[32],"meiotic":[34],"arrest":[35],"for":[36],"17-18":[37],"h":[38],"with":[39,60,65,138,140],"cAMP":[41,44],"analogue,":[42],"dibutyryl":[43],"(dbcAMP).":[45],"Germinal":[46],"vesicle":[47],"breakdown":[48],"(GVB)":[49],"was":[50,58,106,121,149,163],"stimulated":[51],"by":[52,252],"addition":[54],"FSH.":[56,288],"Medium":[57],"supplemented":[59],"either":[61,208],"no":[62,215,231],"macromolecule":[63],"or":[64,78,96,111,117,143,210],"varying":[66],"concentrations":[67],"polyvinylpyrrolidone":[69],"(PVP),":[70],"polyvinylalcohol":[71],"(PVA),":[72],"crystallized":[73,212],"bovine":[74,80,192],"serum":[75,81,194,278],"albumin":[76],"(BSA),":[77],"fetal":[79],"(FBS).":[82],"Oocyte":[83],"all":[86],"FSH-free":[87],"cultures":[88,113],"occurred":[89],"at":[90,134],"a":[91,157,178,197],"frequency":[92,200],"about":[94],"30%":[95],"below.":[97],"High":[98],"frequencies":[99],"achieved":[103],"when":[104,299],"FSH":[105,173,234],"added":[107,204],"macromolecule-free":[109,144],"medium":[110,206],"containing":[114,207],"PVP,":[115],"PVA,":[116],"BSA.":[118],"Crystallized":[119],"BSA":[120,147,162,213,223,258,274],"most":[123],"effective":[124,166],"these":[126],"supporting":[128,168],"stimulation":[129,174],"(94%":[132],"GVB":[133],"3":[135],"mg/ml,":[136],"compared":[137],"72-74%":[139],"synthetic":[141],"polymer-supplemented":[142],"media).":[145],"The":[146],"not":[150,164],"due":[151],"contaminating":[153],"fatty":[154],"acids,":[155],"less":[158],"pure":[159],"fraction":[160,221],"V":[161,222],"as":[165,255,257],"FSH-induced":[169],"maturation.":[170],"FBS":[171,209],"suppressed":[172,224],"dose-dependent":[179],"fashion.":[180],"Sera":[181],"pigs,":[183],"goats,":[184],"horses,":[185],"rats":[187],"also":[189],"inhibitory,":[190],"but":[191,220],"calf":[193],"(BCS)":[195],"permitted":[196],"high":[198],"(80%":[201],"GVB).":[202],"When":[203],"BCS,":[211],"had":[214],"FSH-stimulated":[218],"maturation,":[219],"both":[227],"serum-supplemented":[228],"media.":[229],"Under":[230],"conditions":[232],"did":[233],"stimulate":[235],"cumulus":[238,306],"cell-free":[239],"oocytes.":[240],"These":[241],"results":[242],"demonstrate":[243],"that":[244,260,282],"hormone-induced":[245],"oocyte":[246],"is":[248,295],"supported":[249],"vitro":[251],"nonprotein":[253],"polymers":[254],"well":[256],"behavior":[262],"oocyte-cumulus":[265],"cell":[266],"complex":[267],"depends":[268],"purity":[271],"sample.":[275],"In":[276],"addition,":[277],"contains":[279],"inhibitory":[280],"factors":[281],"suppress":[283],"positive":[285],"response":[286],"Thus,":[289],"choice":[291],"macromolecular":[293],"supplement":[294],"critical":[297],"importance":[298],"testing":[300],"responsiveness":[303]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2014,"cited_by_count":1}],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2016-06-24T00:00:00"}