{"id":"https://openalex.org/W2150093514","doi":"https://doi.org/10.1369/jhc.4a6249.2004","title":"Quantification of In Situ Hybridization Signals in Rat Testes","display_name":"Quantification of In Situ Hybridization Signals in Rat Testes","publication_year":2004,"publication_date":"2004-05-18","ids":{"openalex":"https://openalex.org/W2150093514","doi":"https://doi.org/10.1369/jhc.4a6249.2004","mag":"2150093514","pmid":"https://pubmed.ncbi.nlm.nih.gov/15150290"},"language":"en","primary_location":{"id":"doi:10.1369/jhc.4a6249.2004","doi":"https://doi.org/10.1369/jhc.4a6249.2004","is_oa":false,"landing_page_url":"https://doi.org/10.1369/jhc.4a6249.2004","pdf_url":null,"source":{"id":"https://openalex.org/S30841621","display_name":"Journal of Histochemistry & Cytochemistry","issn_l":"0022-1554","issn":["0022-1554","1551-5044"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320017","host_organization_name":"SAGE Publishing","host_organization_lineage":["https://openalex.org/P4310320017"],"host_organization_lineage_names":["SAGE Publishing"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Histochemistry & Cytochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5006662004","display_name":"Touji Kimura","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I163770644","display_name":"Okayama University","ror":"https://ror.org/02pc6pc55","country_code":"JP","type":"education","lineage":["https://openalex.org/I163770644"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Touji Kimura","raw_affiliation_strings":["Department of Cytology and Histology, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cytology and Histology, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan","institution_ids":["https://openalex.org/I163770644"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5076598044","display_name":"Jun Kosaka","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I163770644","display_name":"Okayama University","ror":"https://ror.org/02pc6pc55","country_code":"JP","type":"education","lineage":["https://openalex.org/I163770644"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Jun Kosaka","raw_affiliation_strings":["Department of Cytology and Histology, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cytology and Histology, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan","institution_ids":["https://openalex.org/I163770644"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5023074362","display_name":"Takako Nomura","orcid":"https://orcid.org/0000-0001-9307-8808","observed_orcids":["https://orcid.org/0000-0001-9307-8808"]},"institutions":[{"id":"https://openalex.org/I163770644","display_name":"Okayama University","ror":"https://ror.org/02pc6pc55","country_code":"JP","type":"education","lineage":["https://openalex.org/I163770644"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Takako Nomura","raw_affiliation_strings":["Department of Cytology and Histology, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cytology and Histology, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan","institution_ids":["https://openalex.org/I163770644"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5059975954","display_name":"Teruo Yamada","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I163770644","display_name":"Okayama University","ror":"https://ror.org/02pc6pc55","country_code":"JP","type":"education","lineage":["https://openalex.org/I163770644"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Teruo Yamada","raw_affiliation_strings":["Department of Cytology and Histology, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cytology and Histology, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan","institution_ids":["https://openalex.org/I163770644"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103560170","display_name":"Yukari Miki","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I163770644","display_name":"Okayama University","ror":"https://ror.org/02pc6pc55","country_code":"JP","type":"education","lineage":["https://openalex.org/I163770644"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Yukari Miki","raw_affiliation_strings":["Department of Cytology and Histology, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cytology and Histology, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan","institution_ids":["https://openalex.org/I163770644"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Koji Takagi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I163770644","display_name":"Okayama University","ror":"https://ror.org/02pc6pc55","country_code":"JP","type":"education","lineage":["https://openalex.org/I163770644"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Koji Takagi","raw_affiliation_strings":["Department of Cytology and Histology, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cytology and Histology, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan","institution_ids":["https://openalex.org/I163770644"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5080910285","display_name":"Takashi Kogami","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I163770644","display_name":"Okayama University","ror":"https://ror.org/02pc6pc55","country_code":"JP","type":"education","lineage":["https://openalex.org/I163770644"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Takashi Kogami","raw_affiliation_strings":["Department of Cytology and Histology, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cytology and Histology, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan","institution_ids":["https://openalex.org/I163770644"]}]},{"author_position":"last","author":{"id":null,"display_name":"Junzo Sasaki","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I163770644","display_name":"Okayama University","ror":"https://ror.org/02pc6pc55","country_code":"JP","type":"education","lineage":["https://openalex.org/I163770644"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Junzo Sasaki","raw_affiliation_strings":["Department of Cytology and Histology, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cytology and Histology, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan","institution_ids":["https://openalex.org/I163770644"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I163770644"],"apc_list":{"value":4400,"currency":"USD","value_usd":4400},"apc_paid":null,"fwci":0.6493,"has_fulltext":false,"cited_by_count":8,"citation_normalized_percentile":{"value":0.76891491,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":"52","issue":"6","first_page":"813","last_page":"820"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10116","display_name":"Sperm and Testicular Function","score":0.8809000253677368,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10116","display_name":"Sperm and Testicular Function","score":0.8809000253677368,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.11299999803304672,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11505","display_name":"Reproductive biology and impacts on aquatic species","score":0.0010999999940395355,"subfield":{"id":"https://openalex.org/subfields/1314","display_name":"Physiology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/rat-testis","display_name":"rat testis","score":0.8149999976158142},{"id":"https://openalex.org/keywords/germ-cell-development","display_name":"germ cell development","score":0.7250000238418579},{"id":"https://openalex.org/keywords/spermatogenesis","display_name":"spermatogenesis","score":0.7480000257492065},{"id":"https://openalex.org/keywords/meiosis","display_name":"meiosis","score":0.6470000147819519},{"id":"https://openalex.org/keywords/spermatocytes","display_name":"spermatocytes","score":0.7300000190734863},{"id":"https://openalex.org/keywords/spermatids","display_name":"spermatids","score":0.9449999928474426},{"id":"https://openalex.org/keywords/spermiogenesis","display_name":"spermiogenesis","score":0.7630000114440918},{"id":"https://openalex.org/keywords/paraffin-sections","display_name":"paraffin sections","score":0.8019999861717224}],"concepts":[{"id":"https://openalex.org/C81439078","wikidata":"https://www.wikidata.org/wiki/Q2304142","display_name":"In situ hybridization","level":4,"score":0.795922040939331},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7297302484512329},{"id":"https://openalex.org/C2780848204","wikidata":"https://www.wikidata.org/wiki/Q3127040","display_name":"Fixative","level":3,"score":0.7040637731552124},{"id":"https://openalex.org/C141561535","wikidata":"https://www.wikidata.org/wiki/Q12565265","display_name":"Telophase","level":5,"score":0.6456363201141357},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.52106112241745},{"id":"https://openalex.org/C67905577","wikidata":"https://www.wikidata.org/wiki/Q215980","display_name":"Ribosomal RNA","level":3,"score":0.5109971761703491},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.4895216226577759},{"id":"https://openalex.org/C2777822432","wikidata":"https://www.wikidata.org/wiki/Q216681","display_name":"In situ","level":2,"score":0.47151243686676025},{"id":"https://openalex.org/C123765429","wikidata":"https://www.wikidata.org/wiki/Q36961","display_name":"Spermatogenesis","level":2,"score":0.4564816951751709},{"id":"https://openalex.org/C148196450","wikidata":"https://www.wikidata.org/wiki/Q21100485","display_name":"Meiosis","level":3,"score":0.45597565174102783},{"id":"https://openalex.org/C53420372","wikidata":"https://www.wikidata.org/wiki/Q22299157","display_name":"Anaphase","level":4,"score":0.4515945613384247},{"id":"https://openalex.org/C74864618","wikidata":"https://www.wikidata.org/wiki/Q2332446","display_name":"Staining","level":2,"score":0.4255028963088989},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.3902384638786316},{"id":"https://openalex.org/C30481170","wikidata":"https://www.wikidata.org/wiki/Q37748","display_name":"Chromosome","level":3,"score":0.3406488299369812},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3088667392730713},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.21766409277915955},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.07830506563186646},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012339","descriptor_name":"RNA, Ribosomal, 28S","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D012671","descriptor_name":"Seminiferous Tubules","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D013091","descriptor_name":"Spermatogenesis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013737","descriptor_name":"Testis","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D013737","descriptor_name":"Testis","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D017208","descriptor_name":"Rats, Wistar","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017403","descriptor_name":"In Situ Hybridization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1369/jhc.4a6249.2004","doi":"https://doi.org/10.1369/jhc.4a6249.2004","is_oa":false,"landing_page_url":"https://doi.org/10.1369/jhc.4a6249.2004","pdf_url":null,"source":{"id":"https://openalex.org/S30841621","display_name":"Journal of Histochemistry & Cytochemistry","issn_l":"0022-1554","issn":["0022-1554","1551-5044"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320017","host_organization_name":"SAGE Publishing","host_organization_lineage":["https://openalex.org/P4310320017"],"host_organization_lineage_names":["SAGE Publishing"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Histochemistry & Cytochemistry","raw_type":"journal-article"},{"id":"pmid:15150290","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/15150290","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320334764","display_name":"Japan Society for the Promotion of Science","ror":"https://ror.org/00hhkn466"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":23,"referenced_works":["https://openalex.org/W334636251","https://openalex.org/W351859556","https://openalex.org/W1564476465","https://openalex.org/W1966446731","https://openalex.org/W1981499286","https://openalex.org/W1997968001","https://openalex.org/W1999396866","https://openalex.org/W1999843227","https://openalex.org/W2013967332","https://openalex.org/W2030396609","https://openalex.org/W2033988932","https://openalex.org/W2057645202","https://openalex.org/W2060476638","https://openalex.org/W2069361610","https://openalex.org/W2069808198","https://openalex.org/W2072290486","https://openalex.org/W2077251269","https://openalex.org/W2081309908","https://openalex.org/W2081676666","https://openalex.org/W2088253473","https://openalex.org/W2159978182","https://openalex.org/W2314345556","https://openalex.org/W4285719527"],"related_works":["https://openalex.org/W7098123287","https://openalex.org/W2001988033","https://openalex.org/W2069361610","https://openalex.org/W1969876315","https://openalex.org/W2007056540","https://openalex.org/W2040794065","https://openalex.org/W4381597658","https://openalex.org/W1967574796","https://openalex.org/W52248404","https://openalex.org/W4210463677"],"abstract_inverted_index":{"We":[0],"performed":[1],"basic":[2],"research":[3],"into":[4],"quantifying":[5],"in":[6,11,42,75,99,139],"situ":[7],"hybridization":[8],"(ISH)":[9],"signals":[10],"rat":[12],"testis,":[13],"a":[14,122],"suitable":[15],"organ":[16],"for":[17,125],"the":[18,39,63,71,128,134],"quantification":[19],"because":[20],"germ":[21],"cells":[22],"undergo":[23],"synchronized":[24],"development":[25],"and":[26,50,87,92,102,107,112,133],"show":[27],"stage-specific":[28],"gene":[29],"expression.":[30],"In":[31],"this":[32],"model":[33],"experiment,":[34],"rRNA":[35,68,119],"was":[36,73,121],"selected":[37],"as":[38],"hybridizable":[40,137],"RNA":[41,138],"paraffin":[43],"sections.":[44,141],"Specimens":[45],"fixed":[46],"with":[47,52,70],"Bouin's":[48],"fixative":[49],"hybridized":[51,69],"digoxygenin-labeled":[53],"probes":[54],"could":[55],"easily":[56],"be":[57],"analyzed":[58],"quantitatively":[59],"through":[60],"\"posterization\"":[61],"of":[62,67,104,127,131,136],"images.":[64],"The":[65,94],"amount":[66],"probe":[72],"greatest":[74],"early":[76,108],"primary":[77,82],"spermatocytes,":[78,83,86],"followed":[79],"by":[80,89],"pachytene":[81],"then":[84,113],"diplotene":[85],"finally":[88],"secondary":[90],"spermatocytes":[91],"spermatids.":[93],"amounts":[95],"reached":[96],"low":[97],"levels":[98,135],"metaphase,":[100],"anaphase,":[101],"telophase":[103],"meiotic":[105],"division":[106],"step":[109],"1":[110],"spermatids,":[111],"slightly":[114],"increased":[115],"during":[116],"spermiogenesis.":[117],"ISH":[118],"staining":[120],"useful":[123],"parameter":[124],"evaluation":[126],"quantitative":[129],"analysis":[130],"mRNA":[132],"tissue":[140]},"counts_by_year":[{"year":2018,"cited_by_count":2},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}