{"id":"https://openalex.org/W2149618403","doi":null,"title":"Prolonged retention of estradiol by human breast cancer cells in tissue culture.","display_name":"Prolonged retention of estradiol by human breast cancer cells in tissue culture.","publication_year":1979,"publication_date":"1979-09-01","ids":{"openalex":"https://openalex.org/W2149618403","mag":"2149618403","pmid":"https://pubmed.ncbi.nlm.nih.gov/476661"},"language":"en","primary_location":{"id":"pmid:476661","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/476661","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer research","raw_type":"Journal Article"},"volume":null,"type":"article","indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5081300060","display_name":"Jeannine S. Strobl","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I193531525","display_name":"George Washington University","ror":"https://ror.org/00y4zzh67","country_code":"US","type":"education","lineage":["https://openalex.org/I193531525"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"J S Strobl","raw_affiliation_strings":["(George Washington University)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"(George Washington University)","institution_ids":["https://openalex.org/I193531525"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5028539104","display_name":"Marc E. Lippman","orcid":"https://orcid.org/0000-0001-5280-4084","observed_orcids":["https://orcid.org/0000-0001-5280-4084"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"M E Lippman","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":3.458,"has_fulltext":false,"cited_by_count":74,"citation_normalized_percentile":{"value":0.93110236,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"39","issue":"9","first_page":"3319","last_page":"27"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10756","display_name":"Estrogen and related hormone effects","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1310","display_name":"Endocrinology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10756","display_name":"Estrogen and related hormone effects","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1310","display_name":"Endocrinology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10183","display_name":"Breast Cancer Treatment Studies","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10686","display_name":"Effects and risks of endocrine disrupting chemicals","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2307","display_name":"Health, Toxicology and Mutagenesis"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/estrogen-binding","display_name":"estrogen binding","score":0.4339999854564667},{"id":"https://openalex.org/keywords/mcf-7-cells","display_name":"MCF-7 cells","score":0.7490000128746033},{"id":"https://openalex.org/keywords/breast-cancer-cells","display_name":"breast cancer cells","score":0.8939999938011169},{"id":"https://openalex.org/keywords/estradiol-metabolites","display_name":"estradiol metabolites","score":0.7179999947547913},{"id":"https://openalex.org/keywords/tamoxifen","display_name":"tamoxifen","score":0.7630000114440918},{"id":"https://openalex.org/keywords/bovine-serum-albumin","display_name":"bovine serum albumin","score":0.8790000081062317},{"id":"https://openalex.org/keywords/nonspecific-binding","display_name":"non-specific binding","score":0.6159999966621399},{"id":"https://openalex.org/keywords/estrogenic-activity","display_name":"estrogenic activity","score":0.6710000038146973},{"id":"https://openalex.org/keywords/thymidine-incorporation","display_name":"thymidine incorporation","score":0.8939999938011169},{"id":"https://openalex.org/keywords/estradiol-metabolism","display_name":"estradiol metabolism","score":0.36500000953674316}],"concepts":[{"id":"https://openalex.org/C25642318","wikidata":"https://www.wikidata.org/wiki/Q1992532","display_name":"Incubation","level":2,"score":0.7537423372268677},{"id":"https://openalex.org/C79879829","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Intracellular","level":2,"score":0.6647303104400635},{"id":"https://openalex.org/C2777164284","wikidata":"https://www.wikidata.org/wiki/Q277954","display_name":"Estrogen","level":2,"score":0.6006857752799988},{"id":"https://openalex.org/C2777108182","wikidata":"https://www.wikidata.org/wiki/Q422464","display_name":"Thymidine","level":3,"score":0.5699505805969238},{"id":"https://openalex.org/C24998067","wikidata":"https://www.wikidata.org/wiki/Q4114622","display_name":"Stimulation","level":2,"score":0.5625872015953064},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5565003156661987},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.5011200904846191},{"id":"https://openalex.org/C2994423619","wikidata":"https://www.wikidata.org/wiki/Q9103","display_name":"Human breast","level":4,"score":0.49003708362579346},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.48131024837493896},{"id":"https://openalex.org/C90703135","wikidata":"https://www.wikidata.org/wiki/Q3287622","display_name":"Tissue culture","level":3,"score":0.45393264293670654},{"id":"https://openalex.org/C143701427","wikidata":"https://www.wikidata.org/wiki/Q193566","display_name":"Incubation period","level":3,"score":0.4459821581840515},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.4419018626213074},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.4033345878124237},{"id":"https://openalex.org/C96232424","wikidata":"https://www.wikidata.org/wiki/Q4118072","display_name":"Cancer cell","level":3,"score":0.4014400839805603},{"id":"https://openalex.org/C121608353","wikidata":"https://www.wikidata.org/wiki/Q12078","display_name":"Cancer","level":2,"score":0.26388847827911377},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.24594604969024658},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.23153617978096008},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.16719672083854675},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D001943","descriptor_name":"Breast Neoplasms","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002855","descriptor_name":"Chromatography, Thin Layer","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004958","descriptor_name":"Estradiol","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011960","descriptor_name":"Receptors, Estrogen","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013629","descriptor_name":"Tamoxifen","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D046508","descriptor_name":"Culture Techniques","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":2,"locations":[{"id":"pmid:476661","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/476661","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer research","raw_type":"Journal Article"},{"id":"mag:2149618403","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/476661","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.8560000061988831,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":11,"referenced_works":["https://openalex.org/W1495332006","https://openalex.org/W1775749144","https://openalex.org/W1997218268","https://openalex.org/W1997402760","https://openalex.org/W2014943128","https://openalex.org/W2111706754","https://openalex.org/W2122706524","https://openalex.org/W2124709411","https://openalex.org/W2255395527","https://openalex.org/W2278354703","https://openalex.org/W2282023379"],"related_works":["https://openalex.org/W1996282495","https://openalex.org/W2067815430","https://openalex.org/W2149207074","https://openalex.org/W1992277128","https://openalex.org/W2049879408","https://openalex.org/W1533558773","https://openalex.org/W2056908246","https://openalex.org/W2185338443","https://openalex.org/W2034237779","https://openalex.org/W4231689190"],"abstract_inverted_index":{"Conditions":[0],"are":[1,11],"described":[2],"under":[3],"which":[4],"prolonged":[5],"estradiol":[6,197,228,312],"retention":[7,320],"and":[8,38,50,92,105,108,145,164,198,264,273,316,334],"estrogenic":[9,203],"activity":[10,204],"observed":[12,214],"in":[13,18,78,84,95,123,186,231,258,270,296,326,332,342],"human":[14,80],"breast":[15,81],"cancer":[16,82],"cells":[17,22,83,193,279,297,323],"tissue":[19,85],"culture.":[20],"The":[21,59,66,120,132,153],"were":[23,40,69],"incubated":[24],"for":[25],"three":[26],"hr":[27,54,150,167,176,218],"with":[28,42,57,194,281,299],"a":[29,208,271],"physiological":[30],"concentration":[31,89,102,122,135,155],"of":[32,46,75,90,103,113,116,136,151,156,168,177,191,210,237,292,329,338],"[3H]estradiol":[33,91,93,104,106,121,159,183,245,266,294],"(3":[34],"to":[35,71,143],"5":[36,195],"nM)":[37],"then":[39],"washed":[41,280,298],"3":[43],"successive":[44],"exchanges":[45,306],"medium":[47,126,233,260,282,300,305],"3,":[48],"17,":[49],"24":[51,250],"or":[52,224,285],"48":[53,175,277],"following":[55,67,148],"incubation":[56,190],"[3H]estradiol.":[58],"total":[60,133],"wash":[61,127,144,201],"period":[62],"was":[63,128,138,160,171,184,213,229,240,246,256,268],"78":[64,149,166,217],"hr.":[65,251],"parameters":[68],"monitored":[70],"assess":[72],"the":[73,88,96,100,110,124,187,192,216,232,235,259,293,343],"duration":[74],"estrogen":[76,319],"action":[77],"MCF-7":[79,314],"culture;":[86],"(a)":[87],"metabolites":[94],"media":[97],"washes;":[98],"(b)":[99],"intracellular":[101,134,154],"metabolites;":[107],"(c)":[109],"time":[111],"course":[112],"estradiol-enhanced":[114],"rates":[115],"radiolabeled":[117],"thymidine":[118,211],"incorporation.":[119],"final":[125],"approximately":[129],"0.05":[130],"nM.":[131,173],"tritium":[137],"about":[139],"50":[140],"nM":[141,147,196],"prior":[142],"9":[146],"wash.":[152],"specifically":[157,181,243],"bound":[158,182,244],"initially":[161],"18":[162],"nM,":[163],"after":[165],"wash,":[169,178],"it":[170],"2.8":[172],"After":[174,276],"nearly":[179],"all":[180],"present":[185,295],"nucleus.":[188],"Following":[189],"an":[199],"identical":[200],"procedure,":[202],"as":[205],"measured":[206],"by":[207,321],"stimulation":[209],"incorporation":[212],"throughout":[215],"monitored.":[219],"When":[220,252],"10(-6)":[221],"M":[222,226],"tamoxifen":[223],"10(-7)":[225],"unlabeled":[227],"included":[230,257],"washes,":[234,261],"washout":[236],"nonspecific":[238],"binding":[239,267],"unaffected;":[241],"however,":[242],"essentially":[247],"eliminated":[248],"within":[249],"bovine":[253,287,339],"serum":[254,288,340],"albumin":[255,289,341],"total,":[262],"nonspecific,":[263],"specific":[265],"reduced":[269],"parallel":[272],"dose-dependent":[274],"fashion.":[275],"hr,":[278],"containing":[283],"3.5":[284],"7%":[286],"contained":[290],"one-tenth":[291],"alone.":[301],"We":[302],"conclude":[303],"that":[304,318],"alone":[307],"do":[308],"not":[309],"effectively":[310],"remove":[311],"from":[313],"cells,":[315],"suggest":[317],"estrogen-responsive":[322],"may":[324,345],"mask":[325],"vitro":[327],"assessments":[328],"such":[330],"responsiveness":[331],"this":[333,347],"other":[335],"systems.":[336],"Inclusion":[337],"washes":[344],"alleviate":[346],"problem.":[348]},"counts_by_year":[{"year":2023,"cited_by_count":1},{"year":2020,"cited_by_count":2},{"year":2017,"cited_by_count":6}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}