{"id":"https://openalex.org/W2149495186","doi":"https://doi.org/10.1093/jexbot/51.343.197","title":"ABA activation of an MBP kinase in Pisum sativum epidermal peels correlates with stomatal responses to ABA","display_name":"ABA activation of an MBP kinase in Pisum sativum epidermal peels correlates with stomatal responses to ABA","publication_year":2000,"publication_date":"2000-02-01","ids":{"openalex":"https://openalex.org/W2149495186","doi":"https://doi.org/10.1093/jexbot/51.343.197","mag":"2149495186","pmid":"https://pubmed.ncbi.nlm.nih.gov/10938826"},"language":"en","primary_location":{"id":"doi:10.1093/jexbot/51.343.197","doi":"https://doi.org/10.1093/jexbot/51.343.197","is_oa":false,"landing_page_url":"https://doi.org/10.1093/jexbot/51.343.197","pdf_url":null,"source":{"id":"https://openalex.org/S32610980","display_name":"Journal of Experimental Botany","issn_l":"0022-0957","issn":["0022-0957","1460-2431"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Experimental Botany","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5113825919","display_name":"Edward C. Burnett","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I178535277","display_name":"University of the West of England","ror":"https://ror.org/02nwg5t34","country_code":"GB","type":"education","lineage":["https://openalex.org/I178535277"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Edward C. Burnett","raw_affiliation_strings":["Department of Biological and Biomedical Sciences, University of the West of England (UWE), Bristol, UK","Department of Biological and Biomedical Sciences, University of the West of England (UWE), Bristol, Coldharbour Lane, Bristol BS16 1QY, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological and Biomedical Sciences, University of the West of England (UWE), Bristol, UK","institution_ids":["https://openalex.org/I178535277"]},{"raw_affiliation_string":"Department of Biological and Biomedical Sciences, University of the West of England (UWE), Bristol, Coldharbour Lane, Bristol BS16 1QY, UK","institution_ids":["https://openalex.org/I178535277"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5065422672","display_name":"Radhika Desikan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I178535277","display_name":"University of the West of England","ror":"https://ror.org/02nwg5t34","country_code":"GB","type":"education","lineage":["https://openalex.org/I178535277"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Radhika Desikan","raw_affiliation_strings":["Department of Biological and Biomedical Sciences, University of the West of England (UWE), Bristol, Coldharbour Lane, Bristol BS16 1QY, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological and Biomedical Sciences, University of the West of England (UWE), Bristol, Coldharbour Lane, Bristol BS16 1QY, UK","institution_ids":["https://openalex.org/I178535277"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Rosita C. Moser","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I178535277","display_name":"University of the West of England","ror":"https://ror.org/02nwg5t34","country_code":"GB","type":"education","lineage":["https://openalex.org/I178535277"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Rosita C. Moser","raw_affiliation_strings":["Department of Biological and Biomedical Sciences, University of the West of England (UWE), Bristol, Coldharbour Lane, Bristol BS16 1QY, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological and Biomedical Sciences, University of the West of England (UWE), Bristol, Coldharbour Lane, Bristol BS16 1QY, UK","institution_ids":["https://openalex.org/I178535277"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5074567918","display_name":"Steven J. Neill","orcid":"https://orcid.org/0000-0001-7541-6121","observed_orcids":["https://orcid.org/0000-0001-7541-6121"]},"institutions":[{"id":"https://openalex.org/I178535277","display_name":"University of the West of England","ror":"https://ror.org/02nwg5t34","country_code":"GB","type":"education","lineage":["https://openalex.org/I178535277"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Steven J. Neill","raw_affiliation_strings":["Department of Biological and Biomedical Sciences, University of the West of England (UWE), Bristol, Coldharbour Lane, Bristol BS16 1QY, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological and Biomedical Sciences, University of the West of England (UWE), Bristol, Coldharbour Lane, Bristol BS16 1QY, UK","institution_ids":["https://openalex.org/I178535277"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I178535277"],"apc_list":{"value":3292,"currency":"GBP","value_usd":4341},"apc_paid":null,"fwci":13.1497,"has_fulltext":false,"cited_by_count":132,"citation_normalized_percentile":{"value":0.98695588,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":99},"biblio":{"volume":"51","issue":"343","first_page":"197","last_page":"205"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10014","display_name":"Plant Stress Responses and Tolerance","score":0.9965999722480774,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10014","display_name":"Plant Stress Responses and Tolerance","score":0.9965999722480774,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11098","display_name":"Seed Germination and Physiology","score":0.0010000000474974513,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10184","display_name":"Plant Molecular Biology Research","score":0.00039999998989515007,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/abscisic-acid","display_name":"abscisic acid","score":0.9470000267028809},{"id":"https://openalex.org/keywords/myelin-basic-protein-kinase","display_name":"myelin basic protein kinase","score":0.9330000281333923},{"id":"https://openalex.org/keywords/mitogen-activated-protein-kinase","display_name":"mitogen-activated protein kinase","score":0.8980000019073486},{"id":"https://openalex.org/keywords/pisum-sativum","display_name":"Pisum sativum","score":0.8339999914169312},{"id":"https://openalex.org/keywords/stomatal-closure","display_name":"stomatal closure","score":0.9350000023841858},{"id":"https://openalex.org/keywords/guard-cells","display_name":"guard cells","score":0.847000002861023},{"id":"https://openalex.org/keywords/tyrosine-phosphorylation","display_name":"tyrosine phosphorylation","score":0.8939999938011169},{"id":"https://openalex.org/keywords/mapk-kinase-inhibition","display_name":"MAPK kinase inhibition","score":0.7630000114440918},{"id":"https://openalex.org/keywords/pd98059","display_name":"PD98059","score":0.8949999809265137},{"id":"https://openalex.org/keywords/plant-stress-signaling","display_name":"plant stress signaling","score":0.3659999966621399}],"concepts":[{"id":"https://openalex.org/C136547172","wikidata":"https://www.wikidata.org/wiki/Q241368","display_name":"Guard cell","level":2,"score":0.8371248841285706},{"id":"https://openalex.org/C97029542","wikidata":"https://www.wikidata.org/wiki/Q281417","display_name":"Protein kinase A","level":3,"score":0.6672744750976562},{"id":"https://openalex.org/C57074206","wikidata":"https://www.wikidata.org/wiki/Q14197552","display_name":"MAPK/ERK pathway","level":3,"score":0.586616039276123},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5223068594932556},{"id":"https://openalex.org/C90934575","wikidata":"https://www.wikidata.org/wiki/Q6593810","display_name":"Mitogen-activated protein kinase kinase","level":4,"score":0.5155487060546875},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.503271758556366},{"id":"https://openalex.org/C184235292","wikidata":"https://www.wikidata.org/wiki/Q421851","display_name":"Kinase","level":2,"score":0.5024294853210449},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4077872633934021},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.36309248208999634},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3318827152252197}],"mesh":[{"descriptor_ui":"D000040","descriptor_name":"Abscisic Acid","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004789","descriptor_name":"Enzyme Activation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010766","descriptor_name":"Phosphorylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014443","descriptor_name":"Tyrosine","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D017871","descriptor_name":"Calcium-Calmodulin-Dependent Protein Kinases","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":false},{"descriptor_ui":"D017871","descriptor_name":"Calcium-Calmodulin-Dependent Protein Kinases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D017871","descriptor_name":"Calcium-Calmodulin-Dependent Protein Kinases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D017931","descriptor_name":"DNA Primers","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018532","descriptor_name":"Pisum sativum","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D038362","descriptor_name":"Glycogen Synthase Kinase 3","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1093/jexbot/51.343.197","doi":"https://doi.org/10.1093/jexbot/51.343.197","is_oa":false,"landing_page_url":"https://doi.org/10.1093/jexbot/51.343.197","pdf_url":null,"source":{"id":"https://openalex.org/S32610980","display_name":"Journal of Experimental Botany","issn_l":"0022-0957","issn":["0022-0957","1460-2431"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Experimental Botany","raw_type":"journal-article"},{"id":"pmid:10938826","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10938826","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of experimental botany","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":23,"referenced_works":["https://openalex.org/W48253385","https://openalex.org/W1584943818","https://openalex.org/W1918775481","https://openalex.org/W1964146094","https://openalex.org/W1966725210","https://openalex.org/W1986570146","https://openalex.org/W1987463062","https://openalex.org/W1987486987","https://openalex.org/W1991607636","https://openalex.org/W2007008833","https://openalex.org/W2035936653","https://openalex.org/W2056054105","https://openalex.org/W2057008723","https://openalex.org/W2061887535","https://openalex.org/W2069361154","https://openalex.org/W2087130904","https://openalex.org/W2089691429","https://openalex.org/W2109050378","https://openalex.org/W2111935441","https://openalex.org/W2128635872","https://openalex.org/W2132767202","https://openalex.org/W2142704168","https://openalex.org/W2150273852"],"related_works":["https://openalex.org/W1584943818","https://openalex.org/W1970112128","https://openalex.org/W7096504712","https://openalex.org/W2150273852","https://openalex.org/W2088473157","https://openalex.org/W2075686106","https://openalex.org/W2132767202","https://openalex.org/W4414014112","https://openalex.org/W1991607636","https://openalex.org/W1974318221"],"abstract_inverted_index":{"In-gel":[0],"protein":[1,7,49,183],"kinase":[2,23,42,50,131,168,184],"assays":[3],"using":[4,105],"myelin":[5],"basic":[6],"(MBP)":[8],"as":[9,56],"substrate":[10],"have":[11],"been":[12],"used":[13,91],"to":[14,92],"demonstrate":[15],"that":[16,157],"abscisic":[17],"acid":[18],"(ABA)":[19],"activates":[20],"an":[21,57,106],"MBP":[22,54],"(AMBP":[24],"kinase)":[25],"in":[26,121,160,174,193,199],"epidermal":[27,122,162,202],"peels":[28,163],"prepared":[29],"from":[30,97],"leaves":[31],"of":[32,36,46,68,118,129,138,148,185],"the":[33,44,69],"Argenteum":[34],"mutant":[35],"pea,":[37],"Pisum":[38],"sativum":[39],"L.":[40],"AMBP":[41,130,167],"has":[43],"characteristics":[45],"a":[47,94,110,135,182],"mitogen-activated":[48],"(MAPK):":[51],"it":[52,60,66],"utilizes":[53],"preferentially":[55],"artificial":[58],"substrate,":[59],"is":[61,67,83],"rapidly":[62],"and":[63,82,101,103,125,141,152],"transiently":[64],"activated,":[65],"appropriate":[70],"size":[71],"(molecular":[72],"weight":[73],"c.":[74,186],"45":[75,119],"kDa),":[76],"requires":[77],"tyrosine":[78,84],"phosphorylation":[79],"for":[80],"activity":[81],"phosphorylated":[85],"upon":[86],"activation.":[87,166],"Reverse":[88],"transcription-PCR":[89],"was":[90,169,189],"generate":[93],"previously-cloned":[95],"MAPK":[96,112,139,143,165],"guard":[98,126,175,195],"cells,":[99,196],"epidermis":[100],"mesophyll":[102,124,200],"immunoblotting":[104],"antibody":[107],"raised":[108],"against":[109],"mammalian":[111],"detected":[113],"MAPK-related":[114],"proteins,":[115],"including":[116],"one":[117],"kDa,":[120],"peels,":[123],"cells.":[127],"Inhibition":[128],"activation":[132],"by":[133,172,178,191],"PD98059,":[134],"specific":[136],"inhibitor":[137],"kinase,":[140],"thus":[142],"activation,":[144],"correlated":[145],"with":[146],"PD98059-inhibition":[147],"ABA-induced":[149],"stomatal":[150],"closure":[151],"dehydrin":[153],"gene":[154],"expression,":[155],"suggesting":[156],"ABA":[158,173,192],"effects":[159],"pea":[161],"require":[164],"not":[170,198],"activated":[171,190],"cells":[176],"isolated":[177,194],"enzyme":[179],"treatment.":[180],"However,":[181],"43":[187],"kDa":[188],"but":[197],"or":[201],"tissue.":[203]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":4},{"year":2023,"cited_by_count":3},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":4},{"year":2020,"cited_by_count":3},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":4},{"year":2017,"cited_by_count":8},{"year":2016,"cited_by_count":6},{"year":2014,"cited_by_count":5},{"year":2013,"cited_by_count":4},{"year":2012,"cited_by_count":4}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}