{"id":"https://openalex.org/W2149052736","doi":"https://doi.org/10.1134/s0006297913120055","title":"Internal initiation of polyuridylic acid translation in bacterial cell-free system","display_name":"Internal initiation of polyuridylic acid translation in bacterial cell-free system","publication_year":2013,"publication_date":"2013-12-01","ids":{"openalex":"https://openalex.org/W2149052736","doi":"https://doi.org/10.1134/s0006297913120055","mag":"2149052736","pmid":"https://pubmed.ncbi.nlm.nih.gov/24460969"},"language":"en","primary_location":{"id":"doi:10.1134/s0006297913120055","doi":"https://doi.org/10.1134/s0006297913120055","is_oa":false,"landing_page_url":"https://doi.org/10.1134/s0006297913120055","pdf_url":null,"source":{"id":"https://openalex.org/S71215691","display_name":"Biochemistry (Moscow)","issn_l":"0006-2979","issn":["0006-2979","0320-9725","1608-3040"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320267","host_organization_name":"Pleiades Publishing","host_organization_lineage":["https://openalex.org/P4310320267","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Pleiades Publishing","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry (Moscow)","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5047261073","display_name":"Evgeny A. Sogorin","orcid":"https://orcid.org/0000-0001-7814-4563","observed_orcids":["https://orcid.org/0000-0001-7814-4563"]},"institutions":[{"id":"https://openalex.org/I1313323035","display_name":"Russian Academy of Sciences","ror":"https://ror.org/05qrfxd25","country_code":"RU","type":"government","lineage":["https://openalex.org/I1313323035"]},{"id":"https://openalex.org/I4210137602","display_name":"Institute of Protein Research","ror":"https://ror.org/0489f0s93","country_code":"RU","type":"facility","lineage":["https://openalex.org/I1313323035","https://openalex.org/I4210129690","https://openalex.org/I4210137602"]}],"countries":["RU"],"is_corresponding":false,"raw_author_name":"E. A. Sogorin","raw_affiliation_strings":["Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia. spirin@vega.protres.ru","Institute of Protein Research, Russian Academy of Sciences, ul. Institutskaya 4, 142290, Pushchino, Moscow Region, Russia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia. spirin@vega.protres.ru","institution_ids":["https://openalex.org/I1313323035","https://openalex.org/I4210137602"]},{"raw_affiliation_string":"Institute of Protein Research, Russian Academy of Sciences, ul. Institutskaya 4, 142290, Pushchino, Moscow Region, Russia","institution_ids":["https://openalex.org/I1313323035","https://openalex.org/I4210137602"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5000682688","display_name":"Sultan Ch. Agalarov","orcid":"https://orcid.org/0000-0002-2567-447X","observed_orcids":["https://orcid.org/0000-0002-2567-447X"]},"institutions":[{"id":"https://openalex.org/I1313323035","display_name":"Russian Academy of Sciences","ror":"https://ror.org/05qrfxd25","country_code":"RU","type":"government","lineage":["https://openalex.org/I1313323035"]},{"id":"https://openalex.org/I4210137602","display_name":"Institute of Protein Research","ror":"https://ror.org/0489f0s93","country_code":"RU","type":"facility","lineage":["https://openalex.org/I1313323035","https://openalex.org/I4210129690","https://openalex.org/I4210137602"]}],"countries":["RU"],"is_corresponding":false,"raw_author_name":"S. Ch. Agalarov","raw_affiliation_strings":["Institute of Protein Research, Russian Academy of Sciences, ul. Institutskaya 4, 142290, Pushchino, Moscow Region, Russia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Protein Research, Russian Academy of Sciences, ul. Institutskaya 4, 142290, Pushchino, Moscow Region, Russia","institution_ids":["https://openalex.org/I1313323035","https://openalex.org/I4210137602"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5015282605","display_name":"Alexander S. Spirin","orcid":"https://orcid.org/0009-0007-1819-6477","observed_orcids":["https://orcid.org/0009-0007-1819-6477"]},"institutions":[{"id":"https://openalex.org/I1313323035","display_name":"Russian Academy of Sciences","ror":"https://ror.org/05qrfxd25","country_code":"RU","type":"government","lineage":["https://openalex.org/I1313323035"]},{"id":"https://openalex.org/I4210137602","display_name":"Institute of Protein Research","ror":"https://ror.org/0489f0s93","country_code":"RU","type":"facility","lineage":["https://openalex.org/I1313323035","https://openalex.org/I4210129690","https://openalex.org/I4210137602"]}],"countries":["RU"],"is_corresponding":true,"raw_author_name":"A. S. Spirin","raw_affiliation_strings":["Institute of Protein Research, Russian Academy of Sciences, ul. Institutskaya 4, 142290, Pushchino, Moscow Region, Russia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Protein Research, Russian Academy of Sciences, ul. Institutskaya 4, 142290, Pushchino, Moscow Region, Russia","institution_ids":["https://openalex.org/I1313323035","https://openalex.org/I4210137602"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5015282605"],"corresponding_institution_ids":["https://openalex.org/I1313323035","https://openalex.org/I4210137602"],"apc_list":null,"apc_paid":null,"fwci":0.1008,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":{"value":0.37913095,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"78","issue":"12","first_page":"1354","last_page":"1357"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11482","display_name":"RNA modifications and cancer","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/polyuridylic-acid","display_name":"polyuridylic acid","score":0.8560000061988831},{"id":"https://openalex.org/keywords/internal-translation-initiation","display_name":"internal translation initiation","score":0.699999988079071},{"id":"https://openalex.org/keywords/escherichia-coli-ribosome","display_name":"Escherichia coli ribosome","score":0.9319999814033508},{"id":"https://openalex.org/keywords/polyphenylalanine-synthesis","display_name":"polyphenylalanine synthesis","score":0.8939999938011169},{"id":"https://openalex.org/keywords/shine-dalgarno-sequence","display_name":"Shine-Dalgarno sequence","score":0.9049999713897705},{"id":"https://openalex.org/keywords/start-codon","display_name":"start codon","score":0.7480000257492065}],"concepts":[{"id":"https://openalex.org/C149364088","wikidata":"https://www.wikidata.org/wiki/Q185917","display_name":"Translation (biology)","level":4,"score":0.6737098693847656},{"id":"https://openalex.org/C2992676002","wikidata":"https://www.wikidata.org/wiki/Q79798","display_name":"Translation system","level":3,"score":0.49927425384521484},{"id":"https://openalex.org/C10419127","wikidata":"https://www.wikidata.org/wiki/Q1989580","display_name":"Cell-free system","level":3,"score":0.41570383310317993},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.405823290348053},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3515264689922333},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.27906864881515503},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.07093015313148499},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.06909459829330444},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.06719812750816345},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D002474","descriptor_name":"Cell-Free System","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D009841","descriptor_name":"Oligonucleotides","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D011072","descriptor_name":"Poly U","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D011072","descriptor_name":"Poly U","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D012260","descriptor_name":"Ribonucleases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012270","descriptor_name":"Ribosomes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D014176","descriptor_name":"Protein Biosynthesis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018387","descriptor_name":"Codon, Initiator","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019793","descriptor_name":"Fluorescein","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D024506","descriptor_name":"5' Flanking Region","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1134/s0006297913120055","doi":"https://doi.org/10.1134/s0006297913120055","is_oa":false,"landing_page_url":"https://doi.org/10.1134/s0006297913120055","pdf_url":null,"source":{"id":"https://openalex.org/S71215691","display_name":"Biochemistry (Moscow)","issn_l":"0006-2979","issn":["0006-2979","0320-9725","1608-3040"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320267","host_organization_name":"Pleiades Publishing","host_organization_lineage":["https://openalex.org/P4310320267","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Pleiades Publishing","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry (Moscow)","raw_type":"journal-article"},{"id":"pmid:24460969","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/24460969","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry. Biokhimiia","raw_type":"Journal Article"},{"id":"mag:3217492613","doi":null,"is_oa":false,"landing_page_url":"https://naukarus.com/vnutrennyaya-initsiatsiya-translyatsii-na-poliuridilovoy-posledovatelnosti-matrichnogo-poliribonukleotida-v-bakterialnoy-","pdf_url":null,"source":{"id":"https://openalex.org/S71215691","display_name":"Biochemistry (Moscow)","issn_l":"0006-2979","issn":["0006-2979","0320-9725","1608-3040"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320267","host_organization_name":"Pleiades Publishing","host_organization_lineage":["https://openalex.org/P4310320267","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Pleiades Publishing","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"\u0411\u0438\u043e\u0445\u0438\u043c\u0438\u044f","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":8,"referenced_works":["https://openalex.org/W135666957","https://openalex.org/W1490363862","https://openalex.org/W1974469744","https://openalex.org/W1983446883","https://openalex.org/W2039335276","https://openalex.org/W2041638729","https://openalex.org/W2060528969","https://openalex.org/W2166064969"],"related_works":["https://openalex.org/W3217492613","https://openalex.org/W1984316935","https://openalex.org/W1594806111","https://openalex.org/W1750762778","https://openalex.org/W2397202502","https://openalex.org/W2418689696","https://openalex.org/W2031560257","https://openalex.org/W2442152046","https://openalex.org/W6962503801","https://openalex.org/W2216212905"],"abstract_inverted_index":{"The":[0],"task":[1],"of":[2,19,41,96,107,134,143,146,158],"the":[3,9,12,38,42,51,60,67,69,94,97,105,111,116,124,139,144,156],"present":[4],"work":[5],"was":[6,89,102,122,127],"to":[7,93],"answer":[8],"question:":[10],"is":[11],"free":[13,48,120],"5'-end":[14,49,56,77,95,121,145],"needed":[15],"for":[16],"effective":[17],"translation":[18,63,137],"a":[20,29,159],"model":[21],"polyribonucleotide":[22],"template":[23,39,98,113,147],"-":[24,27],"polyuridylic":[25,44,52,99,117,140],"acid":[26,45,53,118],"in":[28,59,155],"bacterial":[30,61,130],"(E.":[31],"coli)":[32],"cell-free":[33,62],"system?":[34],"For":[35],"this":[36],"purpose,":[37],"activities":[40],"original":[43],"with":[46,54,72,119],"its":[47,76,83],"and":[50,78,114,162],"blocked":[55],"were":[57],"compared":[58],"system.":[64],"To":[65],"block":[66],"5'-end,":[68],"cytidylic":[70],"oligodeoxyribonucleotide":[71],"fluorescein":[73],"residue":[74],"at":[75,82,138],"uridylic":[79],"oligoribonucleotide":[80],"sequence":[81,141,161],"3'-end,":[84],"schematically":[85],"described":[86],"as":[87],"FAM(dC)10(rU)50,":[88],"covalently":[90],"attached":[91],"(ligated)":[92],"acid.":[100],"It":[101,126],"shown":[103],"that":[104,129],"efficiency":[106],"polyphenylalanine":[108],"synthesis":[109],"on":[110,115],"5'-blocked":[112],"virtually":[123],"same.":[125],"concluded":[128],"ribosomes":[131],"are":[132],"capable":[133],"effectively":[135],"initiating":[136],"independently":[142],"polyribonucleotide,":[148],"i.e.":[149],"via":[150],"an":[151],"internal":[152],"initiation":[153],"mechanism,":[154],"absence":[157],"Shine-Dalgarno":[160],"AUG":[163],"start":[164],"codon.":[165]},"counts_by_year":[{"year":2016,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}