{"id":"https://openalex.org/W2148236154","doi":"https://doi.org/10.1002/chem.201202495","title":"Site\u2010Specific Labeling of Proteins with a Chemically Stable, High\u2010Affinity Tag for Protein Study","display_name":"Site\u2010Specific Labeling of Proteins with a Chemically Stable, High\u2010Affinity Tag for Protein Study","publication_year":2012,"publication_date":"2012-11-14","ids":{"openalex":"https://openalex.org/W2148236154","doi":"https://doi.org/10.1002/chem.201202495","mag":"2148236154","pmid":"https://pubmed.ncbi.nlm.nih.gov/23154941"},"language":"en","primary_location":{"id":"doi:10.1002/chem.201202495","doi":"https://doi.org/10.1002/chem.201202495","is_oa":false,"landing_page_url":"https://doi.org/10.1002/chem.201202495","pdf_url":null,"source":{"id":"https://openalex.org/S68911691","display_name":"Chemistry - A European Journal","issn_l":"0947-6539","issn":["0947-6539","1521-3765"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Chemistry \u2013 A European Journal","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5102714782","display_name":"Yin Yang","orcid":"https://orcid.org/0009-0005-6855-8494","observed_orcids":["https://orcid.org/0009-0005-6855-8494"]},"institutions":[{"id":"https://openalex.org/I205237279","display_name":"Nankai University","ror":"https://ror.org/01y1kjr75","country_code":"CN","type":"education","lineage":["https://openalex.org/I205237279"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yin Yang","raw_affiliation_strings":["State Key Laboratory of Elemento-organic Chemistry, Nankai University, Weijin Road 94, Tianjin 300071, PR China","These authors contributed equally to this work"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"State Key Laboratory of Elemento-organic Chemistry, Nankai University, Weijin Road 94, Tianjin 300071, PR China","institution_ids":["https://openalex.org/I205237279"]},{"raw_affiliation_string":"These authors contributed equally to this work","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100349227","display_name":"Qingfeng Li","orcid":"https://orcid.org/0000-0002-5851-9337","observed_orcids":["https://orcid.org/0000-0002-5851-9337"]},"institutions":[{"id":"https://openalex.org/I205237279","display_name":"Nankai University","ror":"https://ror.org/01y1kjr75","country_code":"CN","type":"education","lineage":["https://openalex.org/I205237279"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Qing\u2010Feng Li","raw_affiliation_strings":["State Key Laboratory of Elemento-organic Chemistry, Nankai University, Weijin Road 94, Tianjin 300071 (P.R. China),","These authors contributed equally to this work"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"State Key Laboratory of Elemento-organic Chemistry, Nankai University, Weijin Road 94, Tianjin 300071 (P.R. China),","institution_ids":["https://openalex.org/I205237279"]},{"raw_affiliation_string":"These authors contributed equally to this work","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5013799554","display_name":"Chan Cao","orcid":"https://orcid.org/0000-0001-7023-8418","observed_orcids":["https://orcid.org/0000-0001-7023-8418"]},"institutions":[{"id":"https://openalex.org/I205237279","display_name":"Nankai University","ror":"https://ror.org/01y1kjr75","country_code":"CN","type":"education","lineage":["https://openalex.org/I205237279"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Chan Cao","raw_affiliation_strings":["State Key Laboratory of Elemento-organic Chemistry, Nankai University, Weijin Road 94, Tianjin 300071 (P.R. China),"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"State Key Laboratory of Elemento-organic Chemistry, Nankai University, Weijin Road 94, Tianjin 300071 (P.R. China),","institution_ids":["https://openalex.org/I205237279"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5104305929","display_name":"Feng Huang","orcid":"https://orcid.org/0009-0004-5092-4911","observed_orcids":["https://orcid.org/0009-0004-5092-4911"]},"institutions":[{"id":"https://openalex.org/I205237279","display_name":"Nankai University","ror":"https://ror.org/01y1kjr75","country_code":"CN","type":"education","lineage":["https://openalex.org/I205237279"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Feng Huang","raw_affiliation_strings":["State Key Laboratory of Elemento-organic Chemistry, Nankai University, Weijin Road 94, Tianjin 300071 (P.R. China),"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"State Key Laboratory of Elemento-organic Chemistry, Nankai University, Weijin Road 94, Tianjin 300071 (P.R. China),","institution_ids":["https://openalex.org/I205237279"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5032938196","display_name":"Xun\u2010Cheng Su","orcid":"https://orcid.org/0000-0003-3051-0047","observed_orcids":["https://orcid.org/0000-0003-3051-0047"]},"institutions":[{"id":"https://openalex.org/I205237279","display_name":"Nankai University","ror":"https://ror.org/01y1kjr75","country_code":"CN","type":"education","lineage":["https://openalex.org/I205237279"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Xun\u2010Cheng Su","raw_affiliation_strings":["State Key Laboratory of Elemento-organic Chemistry, Nankai University, Weijin Road 94, Tianjin 300071 (P.R. China),"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"State Key Laboratory of Elemento-organic Chemistry, Nankai University, Weijin Road 94, Tianjin 300071 (P.R. China),","institution_ids":["https://openalex.org/I205237279"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5032938196"],"corresponding_institution_ids":["https://openalex.org/I205237279"],"apc_list":{"value":4640,"currency":"USD","value_usd":4640},"apc_paid":null,"fwci":1.6897,"has_fulltext":false,"cited_by_count":49,"citation_normalized_percentile":{"value":0.81104651,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":92,"max":98},"biblio":{"volume":"19","issue":"3","first_page":"1097","last_page":"1103"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11809","display_name":"Advanced NMR Techniques and Applications","score":0.6664999723434448,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11809","display_name":"Advanced NMR Techniques and Applications","score":0.6664999723434448,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11279","display_name":"Lanthanide and Transition Metal Complexes","score":0.3107999861240387,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12381","display_name":"Electron Spin Resonance Studies","score":0.007000000216066837,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/site-specific-protein-labeling","display_name":"site-specific protein labeling","score":0.9409999847412109},{"id":"https://openalex.org/keywords/paramagnetic-nmr","display_name":"paramagnetic NMR","score":0.6589999794960022},{"id":"https://openalex.org/keywords/thiol-alkylation","display_name":"thiol alkylation","score":0.9419999718666077},{"id":"https://openalex.org/keywords/pseudocontact-shifts","display_name":"pseudocontact shifts","score":0.7379999756813049},{"id":"https://openalex.org/keywords/residual-dipolar-couplings","display_name":"residual dipolar couplings","score":0.9909999966621399},{"id":"https://openalex.org/keywords/paramagnetic-relaxation-enhancement","display_name":"paramagnetic relaxation enhancement","score":0.996999979019165},{"id":"https://openalex.org/keywords/ubiquitin","display_name":"ubiquitin","score":0.7099999785423279},{"id":"https://openalex.org/keywords/protein-ligand-complex","display_name":"protein-ligand complex","score":0.8130000233650208},{"id":"https://openalex.org/keywords/protein-mobility","display_name":"protein mobility","score":0.41100001335144043}],"concepts":[{"id":"https://openalex.org/C124712363","wikidata":"https://www.wikidata.org/wiki/Q188479","display_name":"Paramagnetism","level":2,"score":0.7793788909912109},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.695358395576477},{"id":"https://openalex.org/C201770740","wikidata":"https://www.wikidata.org/wiki/Q19569","display_name":"Lanthanide","level":3,"score":0.643653929233551},{"id":"https://openalex.org/C150769733","wikidata":"https://www.wikidata.org/wiki/Q7315511","display_name":"Residual dipolar coupling","level":3,"score":0.47322049736976624},{"id":"https://openalex.org/C66974803","wikidata":"https://www.wikidata.org/wiki/Q10359898","display_name":"Nuclear magnetic resonance spectroscopy","level":2,"score":0.45257988572120667},{"id":"https://openalex.org/C116569031","wikidata":"https://www.wikidata.org/wiki/Q899107","display_name":"Ligand (biochemistry)","level":3,"score":0.41560280323028564},{"id":"https://openalex.org/C47701112","wikidata":"https://www.wikidata.org/wiki/Q735188","display_name":"Protein structure","level":2,"score":0.41497477889060974},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.3302520513534546},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.25736531615257263},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.21400213241577148},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.1141616702079773},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.11045244336128235},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0},{"id":"https://openalex.org/C145148216","wikidata":"https://www.wikidata.org/wiki/Q36496","display_name":"Ion","level":2,"score":0.0},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008958","descriptor_name":"Models, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011725","descriptor_name":"Pyridines","qualifier_ui":"Q000138","qualifier_name":"chemical synthesis","is_major_topic":false},{"descriptor_ui":"D011725","descriptor_name":"Pyridines","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D013194","descriptor_name":"Staining and Labeling","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D014753","descriptor_name":"Vinyl Compounds","qualifier_ui":"Q000138","qualifier_name":"chemical synthesis","is_major_topic":false},{"descriptor_ui":"D014753","descriptor_name":"Vinyl Compounds","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D015394","descriptor_name":"Molecular Structure","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017354","descriptor_name":"Point Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019906","descriptor_name":"Nuclear Magnetic Resonance, Biomolecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D025801","descriptor_name":"Ubiquitin","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D025801","descriptor_name":"Ubiquitin","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D028581","descriptor_name":"Lanthanoid Series Elements","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D055550","descriptor_name":"Protein Stability","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1002/chem.201202495","doi":"https://doi.org/10.1002/chem.201202495","is_oa":false,"landing_page_url":"https://doi.org/10.1002/chem.201202495","pdf_url":null,"source":{"id":"https://openalex.org/S68911691","display_name":"Chemistry - A European Journal","issn_l":"0947-6539","issn":["0947-6539","1521-3765"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Chemistry \u2013 A European Journal","raw_type":"journal-article"},{"id":"pmid:23154941","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/23154941","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Chemistry (Weinheim an der Bergstrasse, Germany)","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":51,"referenced_works":["https://openalex.org/W141939547","https://openalex.org/W886277281","https://openalex.org/W1965461315","https://openalex.org/W1974708750","https://openalex.org/W1978009461","https://openalex.org/W1989786185","https://openalex.org/W1990255645","https://openalex.org/W1997023393","https://openalex.org/W1999062038","https://openalex.org/W2001020431","https://openalex.org/W2007145419","https://openalex.org/W2008010726","https://openalex.org/W2011397327","https://openalex.org/W2011406305","https://openalex.org/W2013759064","https://openalex.org/W2015247320","https://openalex.org/W2018277210","https://openalex.org/W2019612659","https://openalex.org/W2020556944","https://openalex.org/W2023567213","https://openalex.org/W2044428266","https://openalex.org/W2052373355","https://openalex.org/W2058048068","https://openalex.org/W2063009680","https://openalex.org/W2065933864","https://openalex.org/W2067687461","https://openalex.org/W2081668532","https://openalex.org/W2085671842","https://openalex.org/W2087741460","https://openalex.org/W2088384049","https://openalex.org/W2088993272","https://openalex.org/W2101982331","https://openalex.org/W2105537524","https://openalex.org/W2108154959","https://openalex.org/W2109207791","https://openalex.org/W2109939279","https://openalex.org/W2115418552","https://openalex.org/W2129224362","https://openalex.org/W2134152126","https://openalex.org/W2142794753","https://openalex.org/W2143507008","https://openalex.org/W2144525976","https://openalex.org/W2144925258","https://openalex.org/W2151709132","https://openalex.org/W2159099732","https://openalex.org/W2173566247","https://openalex.org/W2325407743","https://openalex.org/W2951254503","https://openalex.org/W2952888506","https://openalex.org/W4241509856","https://openalex.org/W4245524317"],"related_works":["https://openalex.org/W2017737880","https://openalex.org/W2088384049","https://openalex.org/W2775284236","https://openalex.org/W2163197082","https://openalex.org/W2144925258","https://openalex.org/W4295350421","https://openalex.org/W2143507008","https://openalex.org/W1989786185","https://openalex.org/W2159099732","https://openalex.org/W2602236112"],"abstract_inverted_index":{"Site-specific":[0],"labeling":[1],"of":[2,12,50,92,150],"proteins":[3,51,63],"with":[4,68,105,134],"paramagnetic":[5,21,37],"lanthanides":[6,123],"offers":[7],"unique":[8],"opportunities":[9],"by":[10,24],"virtue":[11],"NMR":[13],"spectroscopy":[14],"in":[15,44,64,87,96,99,132,147],"structural":[16],"biology.":[17],"In":[18,103],"particular,":[19],"these":[20],"data,":[22],"generated":[23],"the":[25,106,137,146],"anisotropic":[26],"paramagnetism":[27],"including":[28],"pseudocontact":[29],"shifts":[30],"(PCS),":[31],"residual":[32],"dipolar":[33],"couplings":[34],"(RDC),":[35],"and":[36,47,52,71,89,98,142],"relaxation":[38],"enhancement":[39],"(PRE),":[40],"are":[41],"highly":[42],"valuable":[43],"structure":[45],"determination":[46],"mobility":[48],"studies":[49],"protein-ligand":[53],"complexes.":[54],"Herein,":[55],"we":[56],"present":[57],"a":[58,65,69,100,117],"new":[59],"way":[60],"to":[61,145],"label":[62],"site-specific":[66],"manner":[67],"high-affinity":[70],"chemically":[72],"stable":[73,141],"tag,":[74],"4-vinyl(pyridine-2,6-diyl)bismethylenenitrilo":[75],"tetrakis(acetic":[76],"acid)":[77],"(4VPyMTA),":[78],"through":[79],"thiol":[80],"alkylation.":[81],"Its":[82],"performance":[83],"has":[84,110,116],"been":[85],"demonstrated":[86],"G47C":[88],"E64C":[90],"mutants":[91],"human":[93],"ubiquitin":[94],"both":[95],"vitro":[97],"crowded":[101],"environment.":[102],"comparison":[104],"published":[107],"tags,":[108],"4VPyMTA":[109],"several":[111],"interesting":[112],"features:":[113],"1)":[114],"it":[115],"very":[118],"high":[119],"binding":[120],"affinity":[121],"for":[122],"(higher":[124],"than":[125],"EDTA),":[126],"2)":[127],"there":[128],"is":[129,140],"no":[130],"heterogeneity":[131],"complexes":[133],"lanthanides,":[135],"3)":[136],"derivatized":[138],"protein":[139],"potentially":[143],"applicable":[144],"situ":[148],"analysis":[149],"proteins.":[151]},"counts_by_year":[{"year":2026,"cited_by_count":3},{"year":2025,"cited_by_count":4},{"year":2024,"cited_by_count":3},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":3},{"year":2018,"cited_by_count":4},{"year":2017,"cited_by_count":6},{"year":2016,"cited_by_count":6},{"year":2015,"cited_by_count":4},{"year":2014,"cited_by_count":6},{"year":2013,"cited_by_count":4}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}
