{"id":"https://openalex.org/W2147802897","doi":"https://doi.org/10.2108/zsj.19.293","title":"Molecular Cloning of a Putative Gastric Chitinase in the Toad Bufo japonicus","display_name":"Molecular Cloning of a Putative Gastric Chitinase in the Toad Bufo japonicus","publication_year":2002,"publication_date":"2002-03-01","ids":{"openalex":"https://openalex.org/W2147802897","doi":"https://doi.org/10.2108/zsj.19.293","mag":"2147802897","pmid":"https://pubmed.ncbi.nlm.nih.gov/12125927"},"language":"en","primary_location":{"id":"doi:10.2108/zsj.19.293","doi":"https://doi.org/10.2108/zsj.19.293","is_oa":true,"landing_page_url":"https://doi.org/10.2108/zsj.19.293","pdf_url":"https://bioone.org/journals/zoological-science/volume-19/issue-3/zsj.19.293/Molecular-Cloning-of-a-Putative-Gastric-Chitinase-in-the-Toad/10.2108/zsj.19.293.pdf","source":{"id":"https://openalex.org/S34358731","display_name":"ZOOLOGICAL SCIENCE","issn_l":"0289-0003","issn":["0289-0003","2212-3830"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","medline","norway-1","poland-40","russia-white-list-2"],"host_organization":"https://openalex.org/P4310313101","host_organization_name":"Zoological Society of Japan","host_organization_lineage":["https://openalex.org/P4310313101"],"host_organization_lineage_names":["Zoological Society of Japan"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Zoological Science","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://bioone.org/journals/zoological-science/volume-19/issue-3/zsj.19.293/Molecular-Cloning-of-a-Putative-Gastric-Chitinase-in-the-Toad/10.2108/zsj.19.293.pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Hiroyuki Oshima","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I150744194","display_name":"Waseda University","ror":"https://ror.org/00ntfnx83","country_code":"JP","type":"education","lineage":["https://openalex.org/I150744194"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Hiroyuki Oshima","raw_affiliation_strings":["Department of Biology, School of Education, Waseda University, Tokyo, Japan","1Department of Biology, School of Education, Waseda University, Nishi-waseda 1-6-1, Shinjuku-ku, Tok"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, School of Education, Waseda University, Tokyo, Japan","institution_ids":["https://openalex.org/I150744194"]},{"raw_affiliation_string":"1Department of Biology, School of Education, Waseda University, Nishi-waseda 1-6-1, Shinjuku-ku, Tok","institution_ids":["https://openalex.org/I150744194"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5056241971","display_name":"Rieko Miyazaki","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I150744194","display_name":"Waseda University","ror":"https://ror.org/00ntfnx83","country_code":"JP","type":"education","lineage":["https://openalex.org/I150744194"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Rieko Miyazaki","raw_affiliation_strings":["1Department of Biology, School of Education, Waseda University, Nishi-waseda 1-6-1, Shinjuku-ku, Tok"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Department of Biology, School of Education, Waseda University, Nishi-waseda 1-6-1, Shinjuku-ku, Tok","institution_ids":["https://openalex.org/I150744194"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5028129169","display_name":"Yoshihide Ohe","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I165735259","display_name":"Gunma University","ror":"https://ror.org/046fm7598","country_code":"JP","type":"education","lineage":["https://openalex.org/I165735259"]},{"id":"https://openalex.org/I4405286117","display_name":"Institute for Molecular and Cellular Regulation, Gunma University","ror":"https://ror.org/015vdva42","country_code":"JP","type":"facility","lineage":["https://openalex.org/I165735259","https://openalex.org/I4405286117"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Yoshihide Ohe","raw_affiliation_strings":["2Institute for Molecular and Cellular Regulation, Gunma University, Maebashi 371-0034, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"2Institute for Molecular and Cellular Regulation, Gunma University, Maebashi 371-0034, Japan","institution_ids":["https://openalex.org/I165735259","https://openalex.org/I4405286117"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5020982818","display_name":"Hiroaki Hayashi","orcid":"https://orcid.org/0000-0002-8836-5068","observed_orcids":["https://orcid.org/0000-0002-8836-5068"]},"institutions":[{"id":"https://openalex.org/I71869616","display_name":"Gunma Prefectural College of Health Sciences","ror":"https://ror.org/048xnxc75","country_code":"JP","type":"education","lineage":["https://openalex.org/I4405285997","https://openalex.org/I71869616"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Hiroaki Hayashi","raw_affiliation_strings":["3Gunma Prefectural College of Health Science, Maebashi 371-0052, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"3Gunma Prefectural College of Health Science, Maebashi 371-0052, Japan","institution_ids":["https://openalex.org/I71869616"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111888320","display_name":"K\u014dsuk\u00e9 Kawamura","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I150744194","display_name":"Waseda University","ror":"https://ror.org/00ntfnx83","country_code":"JP","type":"education","lineage":["https://openalex.org/I150744194"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Kosuke Kawamura","raw_affiliation_strings":["1Department of Biology, School of Education, Waseda University, Nishi-waseda 1-6-1, Shinjuku-ku, Tok"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Department of Biology, School of Education, Waseda University, Nishi-waseda 1-6-1, Shinjuku-ku, Tok","institution_ids":["https://openalex.org/I150744194"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5013407142","display_name":"Saka\u00e9 Kikuyama","orcid":"https://orcid.org/0000-0003-4911-0991","observed_orcids":["https://orcid.org/0000-0003-4911-0991"]},"institutions":[{"id":"https://openalex.org/I150744194","display_name":"Waseda University","ror":"https://ror.org/00ntfnx83","country_code":"JP","type":"education","lineage":["https://openalex.org/I150744194"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Sakae Kikuyama","raw_affiliation_strings":["1Department of Biology, School of Education, Waseda University, Nishi-waseda 1-6-1, Shinjuku-ku, Tok"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Department of Biology, School of Education, Waseda University, Nishi-waseda 1-6-1, Shinjuku-ku, Tok","institution_ids":["https://openalex.org/I150744194"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5013407142"],"corresponding_institution_ids":["https://openalex.org/I150744194"],"apc_list":null,"apc_paid":null,"fwci":0.2868,"has_fulltext":true,"cited_by_count":15,"citation_normalized_percentile":{"value":0.49603126,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":95},"biblio":{"volume":"19","issue":"3","first_page":"293","last_page":"297"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12683","display_name":"Studies on Chitinases and Chitosanases","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12683","display_name":"Studies on Chitinases and Chitosanases","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11131","display_name":"Proteoglycans and glycosaminoglycans research","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/chitinase","display_name":"chitinase","score":0.8650000095367432},{"id":"https://openalex.org/keywords/bufo-japonicus","display_name":"Bufo japonicus","score":0.9430000185966492},{"id":"https://openalex.org/keywords/gastric-enzymes","display_name":"gastric enzymes","score":0.6100000143051147},{"id":"https://openalex.org/keywords/cdna-cloning","display_name":"cDNA cloning","score":0.9670000076293945},{"id":"https://openalex.org/keywords/chitin-binding-domain","display_name":"chitin-binding domain","score":0.8489999771118164},{"id":"https://openalex.org/keywords/catalytic-domain","display_name":"catalytic domain","score":0.9900000095367432},{"id":"https://openalex.org/keywords/degenerate-oligonucleotide-primers","display_name":"degenerate oligonucleotide primers","score":0.7789999842643738},{"id":"https://openalex.org/keywords/cdna-library-screening","display_name":"cDNA library screening","score":0.703000009059906}],"concepts":[{"id":"https://openalex.org/C2780778831","wikidata":"https://www.wikidata.org/wiki/Q424099","display_name":"Chitinase","level":3,"score":0.9090322256088257},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8805595636367798},{"id":"https://openalex.org/C2778166480","wikidata":"https://www.wikidata.org/wiki/Q6453151","display_name":"Toad","level":2,"score":0.7639965415000916},{"id":"https://openalex.org/C187882448","wikidata":"https://www.wikidata.org/wiki/Q283478","display_name":"Complementary DNA","level":3,"score":0.6424083709716797},{"id":"https://openalex.org/C47289529","wikidata":"https://www.wikidata.org/wiki/Q908550","display_name":"Open reading frame","level":4,"score":0.5340988039970398},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.5084980726242065},{"id":"https://openalex.org/C19924922","wikidata":"https://www.wikidata.org/wiki/Q408351","display_name":"Molecular cloning","level":4,"score":0.4901369512081146},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4618386924266815},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4310382306575775},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.2023645043373108},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.19423362612724304},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002025","descriptor_name":"Bufonidae","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D002688","descriptor_name":"Chitinases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D002688","descriptor_name":"Chitinases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013270","descriptor_name":"Stomach","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D015152","descriptor_name":"Blotting, Northern","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017422","descriptor_name":"Sequence Analysis, DNA","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018076","descriptor_name":"DNA, Complementary","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D020133","descriptor_name":"Reverse Transcriptase Polymerase Chain Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.2108/zsj.19.293","doi":"https://doi.org/10.2108/zsj.19.293","is_oa":true,"landing_page_url":"https://doi.org/10.2108/zsj.19.293","pdf_url":"https://bioone.org/journals/zoological-science/volume-19/issue-3/zsj.19.293/Molecular-Cloning-of-a-Putative-Gastric-Chitinase-in-the-Toad/10.2108/zsj.19.293.pdf","source":{"id":"https://openalex.org/S34358731","display_name":"ZOOLOGICAL SCIENCE","issn_l":"0289-0003","issn":["0289-0003","2212-3830"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","medline","norway-1","poland-40","russia-white-list-2"],"host_organization":"https://openalex.org/P4310313101","host_organization_name":"Zoological Society of Japan","host_organization_lineage":["https://openalex.org/P4310313101"],"host_organization_lineage_names":["Zoological Society of Japan"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Zoological Science","raw_type":"journal-article"},{"id":"pmid:12125927","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12125927","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Zoological science","raw_type":"Journal Article"},{"id":"pmh:10.2108/zsj.19.293","doi":null,"is_oa":false,"landing_page_url":"http://www.bioone.org/doi/10.2108/zsj.19.293","pdf_url":null,"source":{"id":"https://openalex.org/S4306402358","display_name":"BioOne Complete (BioOne)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210111627","host_organization_name":"BioOne","host_organization_lineage":["https://openalex.org/I4210111627"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"text"}],"best_oa_location":{"id":"doi:10.2108/zsj.19.293","doi":"https://doi.org/10.2108/zsj.19.293","is_oa":true,"landing_page_url":"https://doi.org/10.2108/zsj.19.293","pdf_url":"https://bioone.org/journals/zoological-science/volume-19/issue-3/zsj.19.293/Molecular-Cloning-of-a-Putative-Gastric-Chitinase-in-the-Toad/10.2108/zsj.19.293.pdf","source":{"id":"https://openalex.org/S34358731","display_name":"ZOOLOGICAL SCIENCE","issn_l":"0289-0003","issn":["0289-0003","2212-3830"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","medline","norway-1","poland-40","russia-white-list-2"],"host_organization":"https://openalex.org/P4310313101","host_organization_name":"Zoological Society of Japan","host_organization_lineage":["https://openalex.org/P4310313101"],"host_organization_lineage_names":["Zoological Society of Japan"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Zoological Science","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320322638","display_name":"Waseda University","ror":"https://ror.org/00ntfnx83"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2147802897.pdf","grobid_xml":"https://content.openalex.org/works/W2147802897.grobid-xml"},"referenced_works_count":11,"referenced_works":["https://openalex.org/W59921735","https://openalex.org/W144053868","https://openalex.org/W1599890405","https://openalex.org/W1987250042","https://openalex.org/W2046609279","https://openalex.org/W2075615693","https://openalex.org/W2090727689","https://openalex.org/W2090919511","https://openalex.org/W2094854944","https://openalex.org/W2104635591","https://openalex.org/W2133111763"],"related_works":["https://openalex.org/W1971740359","https://openalex.org/W2094854944","https://openalex.org/W565124267","https://openalex.org/W2080643155","https://openalex.org/W2411642811","https://openalex.org/W2359110351","https://openalex.org/W2020193449","https://openalex.org/W2792174956","https://openalex.org/W2155992722","https://openalex.org/W2049130933"],"abstract_inverted_index":{"On":[0],"the":[1,12,15,50,55,78,108,124,132,135,144,149,164],"basis":[2],"of":[3,11,14,33,43,54,98,134],"our":[4],"preliminary":[5],"observation":[6],"that":[7,126],"a":[8,20,34,58,69,73,94],"crude":[9],"extract":[10],"stomach":[13],"toad":[16,79,165],"Bufo":[17],"japonicus":[18],"exhibited":[19,104],"chitinase":[21,96,119,125,162],"activity":[22],"with":[23],"its":[24],"optimum":[25],"pH":[26],"around":[27],"3.0,":[28],"we":[29,127],"undertook":[30],"molecular":[31],"cloning":[32],"cDNA":[35,74],"encoding":[36],"this":[37,116,139,157],"putative":[38,95,117,140],"gastric":[39,118,141],"chitinase.":[40],"By":[41],"use":[42],"2":[44,51],"degenerate":[45],"oligonucleotide":[46],"primers":[47],"derived":[48],"from":[49,77,123,131],"conserved":[52],"regions":[53],"vertebrate":[56,110],"chitinases,":[57],"reverse":[59],"transcription-PCR":[60],"product":[61,65],"was":[62,66,85],"obtained.":[63],"This":[64],"used":[67],"as":[68,161],"probe":[70],"to":[71,87,107,120,159],"screen":[72],"library":[75],"constructed":[76],"stomach.":[80,166],"The":[81],"longest":[82],"positive":[83],"clone":[84],"revealed":[86,115],"contain":[88],"an":[89],"open":[90],"reading":[91],"frame":[92],"for":[93],"protein":[97,103,158],"484":[99],"amino":[100],"acids,":[101],"which":[102],"sequence":[105],"similarity":[106],"known":[109],"chitinases.":[111],"Our":[112],"data":[113],"also":[114],"be":[121],"distinct":[122],"had":[128],"previously":[129],"isolated":[130],"pancreas":[133],"same":[136],"species.":[137],"In":[138],"chitinase,":[142],"both":[143],"N-terminal":[145],"catalytic":[146],"domain":[147,152],"and":[148],"C-terminal":[150],"chitin-binding":[151],"were":[153],"perfectly":[154],"conserved,":[155],"suggesting":[156],"function":[160],"in":[163]},"counts_by_year":[{"year":2023,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":2}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}
