{"id":"https://openalex.org/W2147642500","doi":"https://doi.org/10.1128/aem.02525-12","title":"Biochemical Properties and Crystal Structure of a \u03b2-Phenylalanine Aminotransferase from Variovorax paradoxus","display_name":"Biochemical Properties and Crystal Structure of a \u03b2-Phenylalanine Aminotransferase from Variovorax paradoxus","publication_year":2012,"publication_date":"2012-10-20","ids":{"openalex":"https://openalex.org/W2147642500","doi":"https://doi.org/10.1128/aem.02525-12","mag":"2147642500","pmid":"https://pubmed.ncbi.nlm.nih.gov/23087034","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/3536118"},"language":"en","primary_location":{"id":"doi:10.1128/aem.02525-12","doi":"https://doi.org/10.1128/aem.02525-12","is_oa":true,"landing_page_url":"https://doi.org/10.1128/aem.02525-12","pdf_url":"https://aem.asm.org/content/aem/79/1/185.full.pdf","source":{"id":"https://openalex.org/S158228368","display_name":"Applied and Environmental Microbiology","issn_l":"0099-2240","issn":["0099-2240","1070-6291","1098-5336"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied and Environmental Microbiology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://aem.asm.org/content/aem/79/1/185.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5044374043","display_name":"Ciprian G. Crismaru","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I169381384","display_name":"University of Groningen","ror":"https://ror.org/012p63287","country_code":"NL","type":"education","lineage":["https://openalex.org/I169381384"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"Ciprian G. Crismaru","raw_affiliation_strings":["Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands","institution_ids":["https://openalex.org/I169381384"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5074947727","display_name":"Gjalt G. Wybenga","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I169381384","display_name":"University of Groningen","ror":"https://ror.org/012p63287","country_code":"NL","type":"education","lineage":["https://openalex.org/I169381384"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"Gjalt G. Wybenga","raw_affiliation_strings":["Laboratory of Biophysical Chemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Biophysical Chemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands","institution_ids":["https://openalex.org/I169381384"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5006401636","display_name":"Wiktor Szyma\u0144ski","orcid":"https://orcid.org/0000-0002-9754-9248","observed_orcids":["https://orcid.org/0000-0002-9754-9248"]},"institutions":[{"id":"https://openalex.org/I169381384","display_name":"University of Groningen","ror":"https://ror.org/012p63287","country_code":"NL","type":"education","lineage":["https://openalex.org/I169381384"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"Wiktor Szymanski","raw_affiliation_strings":["Center for Systems Chemistry, Stratingh Institute for Chemistry, University of Groningen, Groningen, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Systems Chemistry, Stratingh Institute for Chemistry, University of Groningen, Groningen, The Netherlands","institution_ids":["https://openalex.org/I169381384"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5040139581","display_name":"Hein J. Wijma","orcid":"https://orcid.org/0000-0003-0891-6972","observed_orcids":["https://orcid.org/0000-0003-0891-6972"]},"institutions":[{"id":"https://openalex.org/I169381384","display_name":"University of Groningen","ror":"https://ror.org/012p63287","country_code":"NL","type":"education","lineage":["https://openalex.org/I169381384"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"Hein J. Wijma","raw_affiliation_strings":["Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands","institution_ids":["https://openalex.org/I169381384"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5045104920","display_name":"Bian Wu","orcid":"https://orcid.org/0000-0002-6524-2049","observed_orcids":["https://orcid.org/0000-0002-6524-2049"]},"institutions":[{"id":"https://openalex.org/I169381384","display_name":"University of Groningen","ror":"https://ror.org/012p63287","country_code":"NL","type":"education","lineage":["https://openalex.org/I169381384"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"Bian Wu","raw_affiliation_strings":["Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands","institution_ids":["https://openalex.org/I169381384"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5102981513","display_name":"Sebastian Bartsch","orcid":"https://orcid.org/0000-0003-4428-7501","observed_orcids":["https://orcid.org/0000-0003-4428-7501"]},"institutions":[{"id":"https://openalex.org/I169381384","display_name":"University of Groningen","ror":"https://ror.org/012p63287","country_code":"NL","type":"education","lineage":["https://openalex.org/I169381384"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"Sebastian Bartsch","raw_affiliation_strings":["Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands","institution_ids":["https://openalex.org/I169381384"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5029974505","display_name":"Stefaan M. A. De Wildeman","orcid":"https://orcid.org/0000-0001-8373-092X","observed_orcids":["https://orcid.org/0000-0001-8373-092X"]},"institutions":[{"id":"https://openalex.org/I127558574","display_name":"DSM (Netherlands)","ror":"https://ror.org/00k01kt20","country_code":"NL","type":"company","lineage":["https://openalex.org/I127558574"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"Stefaan de Wildeman","raw_affiliation_strings":["DSM Pharmaceutical Products, Geleen, The Netherlands","DSM Pharmaceutical Products, Geleen, The Netherland,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"DSM Pharmaceutical Products, Geleen, The Netherlands","institution_ids":["https://openalex.org/I127558574"]},{"raw_affiliation_string":"DSM Pharmaceutical Products, Geleen, The Netherland,","institution_ids":["https://openalex.org/I127558574"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5015832633","display_name":"Gerrit J. Poelarends","orcid":"https://orcid.org/0000-0002-6917-6368","observed_orcids":["https://orcid.org/0000-0002-6917-6368"]},"institutions":[{"id":"https://openalex.org/I169381384","display_name":"University of Groningen","ror":"https://ror.org/012p63287","country_code":"NL","type":"education","lineage":["https://openalex.org/I169381384"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"Gerrit J. Poelarends","raw_affiliation_strings":["Department of Pharmaceutical Biology, Groningen Research Institute of Pharmacy, University of Groningen, Groningen, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmaceutical Biology, Groningen Research Institute of Pharmacy, University of Groningen, Groningen, The Netherlands","institution_ids":["https://openalex.org/I169381384"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5087713906","display_name":"Ben L. Feringa","orcid":"https://orcid.org/0000-0003-0588-8435","observed_orcids":["https://orcid.org/0000-0003-0588-8435"]},"institutions":[{"id":"https://openalex.org/I169381384","display_name":"University of Groningen","ror":"https://ror.org/012p63287","country_code":"NL","type":"education","lineage":["https://openalex.org/I169381384"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"Ben L. Feringa","raw_affiliation_strings":["Center for Systems Chemistry, Stratingh Institute for Chemistry, University of Groningen, Groningen, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Systems Chemistry, Stratingh Institute for Chemistry, University of Groningen, Groningen, The Netherlands","institution_ids":["https://openalex.org/I169381384"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5066632471","display_name":"Bauke W. Dijkstra","orcid":"https://orcid.org/0000-0001-9731-6586","observed_orcids":["https://orcid.org/0000-0001-9731-6586"]},"institutions":[{"id":"https://openalex.org/I169381384","display_name":"University of Groningen","ror":"https://ror.org/012p63287","country_code":"NL","type":"education","lineage":["https://openalex.org/I169381384"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"Bauke W. Dijkstra","raw_affiliation_strings":["Laboratory of Biophysical Chemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Biophysical Chemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands","institution_ids":["https://openalex.org/I169381384"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5076930474","display_name":"Dick B. Janssen","orcid":"https://orcid.org/0000-0002-0834-2043","observed_orcids":["https://orcid.org/0000-0002-0834-2043"]},"institutions":[{"id":"https://openalex.org/I169381384","display_name":"University of Groningen","ror":"https://ror.org/012p63287","country_code":"NL","type":"education","lineage":["https://openalex.org/I169381384"]}],"countries":["NL"],"is_corresponding":true,"raw_author_name":"Dick B. Janssen","raw_affiliation_strings":["Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands","institution_ids":["https://openalex.org/I169381384"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5076930474"],"corresponding_institution_ids":["https://openalex.org/I169381384"],"apc_list":null,"apc_paid":null,"fwci":0.5249,"has_fulltext":true,"cited_by_count":34,"citation_normalized_percentile":{"value":0.605482,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"79","issue":"1","first_page":"185","last_page":"195"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.43290001153945923,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.43290001153945923,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.21619999408721924,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12770","display_name":"Amino Acid Enzymes and Metabolism","score":0.19740000367164612,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/variovorax-paradoxus","display_name":"Variovorax paradoxus","score":0.9990000128746033},{"id":"https://openalex.org/keywords/kinetic-resolution","display_name":"kinetic resolution","score":0.6669999957084656},{"id":"https://openalex.org/keywords/enantioselectivity","display_name":"enantioselectivity","score":0.8899999856948853},{"id":"https://openalex.org/keywords/substrate-specificity","display_name":"substrate specificity","score":0.6610000133514404},{"id":"https://openalex.org/keywords/crystal-structure","display_name":"crystal structure","score":0.7450000047683716},{"id":"https://openalex.org/keywords/enzyme-overexpression","display_name":"enzyme overexpression","score":0.35199999809265137},{"id":"https://openalex.org/keywords/nitrogen-source-utilization","display_name":"nitrogen source utilization","score":0.5440000295639038},{"id":"https://openalex.org/keywords/aromatic-amino-acid-metabolism","display_name":"aromatic amino acid metabolism","score":0.3930000066757202}],"concepts":[{"id":"https://openalex.org/C2777431362","wikidata":"https://www.wikidata.org/wiki/Q170545","display_name":"Phenylalanine","level":3,"score":0.6872340440750122},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6034339666366577},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.5921266078948975},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.4641401171684265},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.45041486620903015},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4406737685203552},{"id":"https://openalex.org/C2777269803","wikidata":"https://www.wikidata.org/wiki/Q407727","display_name":"Lyase","level":3,"score":0.42732974886894226},{"id":"https://openalex.org/C2777992278","wikidata":"https://www.wikidata.org/wiki/Q705605","display_name":"Aromatic amino acids","level":3,"score":0.41439077258110046}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000637","descriptor_name":"Transaminases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D000637","descriptor_name":"Transaminases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004269","descriptor_name":"DNA, Bacterial","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004269","descriptor_name":"DNA, Bacterial","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D004275","descriptor_name":"DNA, Ribosomal","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004275","descriptor_name":"DNA, Ribosomal","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008958","descriptor_name":"Models, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008970","descriptor_name":"Molecular Weight","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010649","descriptor_name":"Phenylalanine","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D011487","descriptor_name":"Protein Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D012336","descriptor_name":"RNA, Ribosomal, 16S","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013696","descriptor_name":"Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017422","descriptor_name":"Sequence Analysis, DNA","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018360","descriptor_name":"Crystallography, X-Ray","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D042621","descriptor_name":"Comamonadaceae","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D042621","descriptor_name":"Comamonadaceae","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D042621","descriptor_name":"Comamonadaceae","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D042621","descriptor_name":"Comamonadaceae","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false}],"locations_count":6,"locations":[{"id":"doi:10.1128/aem.02525-12","doi":"https://doi.org/10.1128/aem.02525-12","is_oa":true,"landing_page_url":"https://doi.org/10.1128/aem.02525-12","pdf_url":"https://aem.asm.org/content/aem/79/1/185.full.pdf","source":{"id":"https://openalex.org/S158228368","display_name":"Applied and Environmental Microbiology","issn_l":"0099-2240","issn":["0099-2240","1070-6291","1098-5336"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied and Environmental Microbiology","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:2692596","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/articles/PMC3536118","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:3536118","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3536118","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Appl Environ Microbiol","raw_type":"Text"},{"id":"pmid:23087034","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/23087034","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied and environmental microbiology","raw_type":"Journal Article"},{"id":"pmh:oai:pure.rug.nl:openaire/8ec93485-435f-408d-a0db-655107729572","doi":null,"is_oa":true,"landing_page_url":"https://research.rug.nl/en/publications/8ec93485-435f-408d-a0db-655107729572","pdf_url":null,"source":{"id":"https://openalex.org/S4306400420","display_name":"University of Groningen research database (University of Groningen / Centre for Information Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I169381384","host_organization_name":"University of Groningen","host_organization_lineage":["https://openalex.org/I169381384"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Crismaru, C G, Wybenga, G G, Szymanski, W, Wijma, H J, Wu, B, Bartsch, S, de Wildeman, S, Poelarends, G J, Feringa, B L, Dijkstra, B & Janssen, D B 2013, 'Biochemical Properties and Crystal Structure of a \u03b2-Phenylalanine Aminotransferase from Variovorax paradoxus', Applied and environmental microbiology, vol. 79, no. 1, pp. 185-195. https://doi.org/10.1128/AEM.02525-12","raw_type":"info:eu-repo/semantics/article"},{"id":"pmh:rug:oai:pure.rug.nl:publications/8ec93485-435f-408d-a0db-655107729572","doi":null,"is_oa":true,"landing_page_url":"https://research.rug.nl/en/publications/8ec93485-435f-408d-a0db-655107729572","pdf_url":null,"source":{"id":"https://openalex.org/S4306400420","display_name":"University of Groningen research database (University of Groningen / Centre for Information Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I169381384","host_organization_name":"University of Groningen","host_organization_lineage":["https://openalex.org/I169381384"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Applied and environmental microbiology, 79(1), 185 - 195. AMER SOC MICROBIOLOGY","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":{"id":"doi:10.1128/aem.02525-12","doi":"https://doi.org/10.1128/aem.02525-12","is_oa":true,"landing_page_url":"https://doi.org/10.1128/aem.02525-12","pdf_url":"https://aem.asm.org/content/aem/79/1/185.full.pdf","source":{"id":"https://openalex.org/S158228368","display_name":"Applied and Environmental Microbiology","issn_l":"0099-2240","issn":["0099-2240","1070-6291","1098-5336"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied and Environmental Microbiology","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G8168883959","display_name":"Developing the Next Generation of Biocatalysts for Industrial Chemical Synthesis","funder_award_id":"266025","funder_id":"https://openalex.org/F4320320300","funder_display_name":"European Commission"}],"funders":[{"id":"https://openalex.org/F4320320300","display_name":"European Commission","ror":"https://ror.org/00k4n6c32"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2147642500.pdf","grobid_xml":"https://content.openalex.org/works/W2147642500.grobid-xml"},"referenced_works_count":81,"referenced_works":["https://openalex.org/W273023583","https://openalex.org/W1487753561","https://openalex.org/W1498516814","https://openalex.org/W1524426748","https://openalex.org/W1569386599","https://openalex.org/W1673322472","https://openalex.org/W1865429961","https://openalex.org/W1912159835","https://openalex.org/W1969672293","https://openalex.org/W1977857718","https://openalex.org/W1980879856","https://openalex.org/W1982441054","https://openalex.org/W1983735973","https://openalex.org/W1985936122","https://openalex.org/W1993266050","https://openalex.org/W1999618865","https://openalex.org/W1999728411","https://openalex.org/W2001641653","https://openalex.org/W2004269755","https://openalex.org/W2009911379","https://openalex.org/W2014054174","https://openalex.org/W2017700615","https://openalex.org/W2018640543","https://openalex.org/W2027625642","https://openalex.org/W2030517878","https://openalex.org/W2031473433","https://openalex.org/W2033969841","https://openalex.org/W2034529420","https://openalex.org/W2035266859","https://openalex.org/W2035503835","https://openalex.org/W2038840577","https://openalex.org/W2041571635","https://openalex.org/W2044553926","https://openalex.org/W2053547572","https://openalex.org/W2055666215","https://openalex.org/W2055989425","https://openalex.org/W2056362934","https://openalex.org/W2056708185","https://openalex.org/W2066701330","https://openalex.org/W2074836458","https://openalex.org/W2076690812","https://openalex.org/W2076756285","https://openalex.org/W2077437218","https://openalex.org/W2079387987","https://openalex.org/W2081872485","https://openalex.org/W2091921381","https://openalex.org/W2096064309","https://openalex.org/W2097943773","https://openalex.org/W2098613637","https://openalex.org/W2099099776","https://openalex.org/W2103385238","https://openalex.org/W2103591844","https://openalex.org/W2107677212","https://openalex.org/W2110094991","https://openalex.org/W2114367386","https://openalex.org/W2115792147","https://openalex.org/W2124892883","https://openalex.org/W2127322768","https://openalex.org/W2127335066","https://openalex.org/W2136990015","https://openalex.org/W2138051911","https://openalex.org/W2141885858","https://openalex.org/W2144081223","https://openalex.org/W2147874841","https://openalex.org/W2148081875","https://openalex.org/W2153138245","https://openalex.org/W2154714625","https://openalex.org/W2156164782","https://openalex.org/W2163341755","https://openalex.org/W2165331908","https://openalex.org/W2217403617","https://openalex.org/W2234541988","https://openalex.org/W2398869186","https://openalex.org/W2464068384","https://openalex.org/W2478964621","https://openalex.org/W2804007336","https://openalex.org/W2949079685","https://openalex.org/W4210623056","https://openalex.org/W4211022623","https://openalex.org/W4285719527","https://openalex.org/W4292340308"],"related_works":["https://openalex.org/W1999723020","https://openalex.org/W3015166784","https://openalex.org/W4313529921","https://openalex.org/W1972404670","https://openalex.org/W2059522761","https://openalex.org/W2027625642","https://openalex.org/W2465627392","https://openalex.org/W2972031869","https://openalex.org/W1558359858","https://openalex.org/W2083084722"],"abstract_inverted_index":{"By":[0],"selective":[1],"enrichment,":[2],"we":[3],"isolated":[4],"a":[5,12,25,42,51,57,76,101,206],"bacterium":[6],"that":[7],"can":[8],"use":[9],"\u03b2-phenylalanine":[10,110,181],"as":[11,24,112,119],"sole":[13],"nitrogen":[14],"source.":[15],"It":[16],"was":[17,62,72,191],"identified":[18],"by":[19],"16S":[20],"rRNA":[21],"gene":[22,61],"sequencing":[23,38],"strain":[26,186],"of":[27,41,50,79,95,146,163,167,199],"Variovorax":[28],"paradoxus.":[29],"Enzyme":[30],"assays":[31],"revealed":[32,176],"an":[33],"aminotransferase":[34,53,99,182,202],"activity.":[35],"Partial":[36],"genome":[37],"and":[39,64,74,87,108,115,117,132,166,197,203],"screening":[40],"cosmid":[43],"DNA":[44],"library":[45],"resulted":[46],"in":[47,66,170],"the":[48,92,139,143,164,168,173,180,195],"identification":[49],"1,302-bp":[52],"gene,":[54],"which":[55,210],"encodes":[56],"46,416-Da":[58],"protein.":[59],"The":[60,69,97,122,160,188],"cloned":[63],"overexpressed":[65],"Escherichia":[67],"coli.":[68],"recombinant":[70],"enzyme":[71,123,169],"purified":[73],"showed":[75],"specific":[77],"activity":[78],"17.5":[80],"U":[81,89],"mg(-1)":[82,90],"for":[83],"(S)-\u03b2-phenylalanine":[84,128],"at":[85,91],"30\u00b0C":[86],"33":[88],"optimum":[93],"temperature":[94],"55\u00b0C.":[96],"\u03b2-specific":[98],"exhibits":[100],"broad":[102],"substrate":[103],"range,":[104],"accepting":[105],"ortho-,":[106],"meta-,":[107],"para-substituted":[109],"derivatives":[111,133],"amino":[113,120],"donors":[114],"2-oxoglutarate":[116],"pyruvate":[118],"acceptors.":[121],"is":[124],"highly":[125],"enantioselective":[126],"toward":[127],"(enantioselectivity":[129],"[E],":[130],">100)":[131],"thereof":[134],"with":[135,155,172,209],"different":[136],"substituents":[137],"on":[138],"phenyl":[140],"ring,":[141],"allowing":[142],"kinetic":[144],"resolution":[145],"various":[147],"racemic":[148],"\u03b2-amino":[149,213],"acids":[150,154],"to":[151,179,193,204],"yield":[152],"(R)-\u03b2-amino":[153],">95%":[156],"enantiomeric":[157],"excess":[158],"(ee).":[159],"crystal":[161,189],"structures":[162],"holoenzyme":[165],"complex":[171],"inhibitor":[174],"2-aminooxyacetate":[175],"structural":[177],"similarity":[178],"from":[183],"Mesorhizobium":[184],"sp.":[185],"LUK.":[187],"structure":[190],"used":[192],"rationalize":[194],"stereo-":[196],"regioselectivity":[198],"V.":[200],"paradoxus":[201],"define":[205],"sequence":[207],"motif":[208],"new":[211],"aromatic":[212],"acid-converting":[214],"aminotransferases":[215],"may":[216],"be":[217],"identified.":[218]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":4},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":3},{"year":2018,"cited_by_count":6},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":5},{"year":2015,"cited_by_count":3},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}