{"id":"https://openalex.org/W2147494764","doi":"https://doi.org/10.1111/j.1574-6941.2011.01257.x","title":"The currently used commercial DNA-extraction methods give different results of clostridial and actinobacterial populations derived from human fecal samples","display_name":"The currently used commercial DNA-extraction methods give different results of clostridial and actinobacterial populations derived from human fecal samples","publication_year":2011,"publication_date":"2011-11-18","ids":{"openalex":"https://openalex.org/W2147494764","doi":"https://doi.org/10.1111/j.1574-6941.2011.01257.x","mag":"2147494764","pmid":"https://pubmed.ncbi.nlm.nih.gov/22098067"},"language":"en","primary_location":{"id":"doi:10.1111/j.1574-6941.2011.01257.x","doi":"https://doi.org/10.1111/j.1574-6941.2011.01257.x","is_oa":true,"landing_page_url":"https://doi.org/10.1111/j.1574-6941.2011.01257.x","pdf_url":"https://academic.oup.com/femsec/article-pdf/79/3/697/19525720/79-3-697.pdf","source":{"id":"https://openalex.org/S171282171","display_name":"FEMS Microbiology Ecology","issn_l":"0168-6496","issn":["0168-6496","1574-6941"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-c","jufo-1","ki-jl-1","medline","norway-2","oarl","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEMS Microbiology Ecology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://academic.oup.com/femsec/article-pdf/79/3/697/19525720/79-3-697.pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5032899943","display_name":"Johanna Maukonen","orcid":"https://orcid.org/0000-0003-0539-8807","observed_orcids":["https://orcid.org/0000-0003-0539-8807"]},"institutions":[{"id":"https://openalex.org/I87653560","display_name":"VTT Technical Research Centre of Finland","ror":"https://ror.org/04b181w54","country_code":"FI","type":"nonprofit","lineage":["https://openalex.org/I4210089493","https://openalex.org/I87653560"]}],"countries":["FI"],"is_corresponding":true,"raw_author_name":"Johanna Maukonen","raw_affiliation_strings":["VTT; Biotechnology; Espoo; Finland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"VTT; Biotechnology; Espoo; Finland","institution_ids":["https://openalex.org/I87653560"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5012052942","display_name":"Catarina Sim\u00f5es","orcid":"https://orcid.org/0000-0002-4213-8951","observed_orcids":["https://orcid.org/0000-0002-4213-8951"]},"institutions":[{"id":"https://openalex.org/I87653560","display_name":"VTT Technical Research Centre of Finland","ror":"https://ror.org/04b181w54","country_code":"FI","type":"nonprofit","lineage":["https://openalex.org/I4210089493","https://openalex.org/I87653560"]}],"countries":["FI"],"is_corresponding":false,"raw_author_name":"Catarina Sim\u00f5es","raw_affiliation_strings":["VTT; Biotechnology; Espoo; Finland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"VTT; Biotechnology; Espoo; Finland","institution_ids":["https://openalex.org/I87653560"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5059096687","display_name":"Maria Saarela","orcid":"https://orcid.org/0000-0001-8842-7292","observed_orcids":["https://orcid.org/0000-0001-8842-7292"]},"institutions":[{"id":"https://openalex.org/I87653560","display_name":"VTT Technical Research Centre of Finland","ror":"https://ror.org/04b181w54","country_code":"FI","type":"nonprofit","lineage":["https://openalex.org/I4210089493","https://openalex.org/I87653560"]}],"countries":["FI"],"is_corresponding":false,"raw_author_name":"Maria Saarela","raw_affiliation_strings":["VTT; Biotechnology; Espoo; Finland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"VTT; Biotechnology; Espoo; Finland","institution_ids":["https://openalex.org/I87653560"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5032899943"],"corresponding_institution_ids":["https://openalex.org/I87653560"],"apc_list":{"value":2500,"currency":"GBP","value_usd":3297},"apc_paid":null,"fwci":3.8518,"has_fulltext":true,"cited_by_count":126,"citation_normalized_percentile":{"value":0.9528019,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":95,"max":100},"biblio":{"volume":"79","issue":"3","first_page":"697","last_page":"708"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10066","display_name":"Gut microbiota and health","score":0.9965999722480774,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10066","display_name":"Gut microbiota and health","score":0.9965999722480774,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11288","display_name":"Clostridium difficile and Clostridium perfringens research","score":0.000699999975040555,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11791","display_name":"Microbial Community Ecology and Physiology","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dna-extraction-protocols","display_name":"DNA extraction methods","score":0.8080000281333923},{"id":"https://openalex.org/keywords/human-fecal-microbiota","display_name":"human fecal microbiota","score":0.8500000238418579},{"id":"https://openalex.org/keywords/clostridium","display_name":"Clostridium","score":0.8240000009536743},{"id":"https://openalex.org/keywords/actinobacteria","display_name":"actinobacteria","score":0.890999972820282},{"id":"https://openalex.org/keywords/bacteroides","display_name":"Bacteroides","score":0.878000020980835},{"id":"https://openalex.org/keywords/bifidobacterium","display_name":"Bifidobacterium","score":0.972000002861023},{"id":"https://openalex.org/keywords/sample-storage-conditions","display_name":"sample storage conditions","score":0.6269999742507935},{"id":"https://openalex.org/keywords/mechanical-lysis","display_name":"mechanical lysis","score":0.8349999785423279},{"id":"https://openalex.org/keywords/enzymatic-lysis","display_name":"enzymatic lysis","score":0.9580000042915344},{"id":"https://openalex.org/keywords/bacterial-diversity","display_name":"bacterial diversity","score":0.7540000081062317},{"id":"https://openalex.org/keywords/roseburia","display_name":"Roseburia","score":0.7760000228881836},{"id":"https://openalex.org/keywords/coprococcus","display_name":"Coprococcus","score":0.8370000123977661},{"id":"https://openalex.org/keywords/blautia","display_name":"Blautia","score":0.9319999814033508},{"id":"https://openalex.org/keywords/dorea","display_name":"Dorea","score":0.9789999723434448}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.9220161437988281},{"id":"https://openalex.org/C2778168646","wikidata":"https://www.wikidata.org/wiki/Q133186","display_name":"Bacteroides","level":3,"score":0.743557333946228},{"id":"https://openalex.org/C78063203","wikidata":"https://www.wikidata.org/wiki/Q3062596","display_name":"DNA extraction","level":4,"score":0.6901839971542358},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.6466622352600098},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.5927006602287292},{"id":"https://openalex.org/C2780092757","wikidata":"https://www.wikidata.org/wiki/Q5405346","display_name":"Eubacterium","level":3,"score":0.5890149474143982},{"id":"https://openalex.org/C2781092032","wikidata":"https://www.wikidata.org/wiki/Q327663","display_name":"Clostridium","level":3,"score":0.5501615405082703},{"id":"https://openalex.org/C57409179","wikidata":"https://www.wikidata.org/wiki/Q1218293","display_name":"Lysis","level":2,"score":0.5273215174674988},{"id":"https://openalex.org/C2777392542","wikidata":"https://www.wikidata.org/wiki/Q3174392","display_name":"Human feces","level":3,"score":0.5251768827438354},{"id":"https://openalex.org/C61716771","wikidata":"https://www.wikidata.org/wiki/Q496","display_name":"Feces","level":2,"score":0.5150899291038513},{"id":"https://openalex.org/C124956284","wikidata":"https://www.wikidata.org/wiki/Q918223","display_name":"Fecal coliform","level":3,"score":0.4782090187072754},{"id":"https://openalex.org/C2776706654","wikidata":"https://www.wikidata.org/wiki/Q3941444","display_name":"Roseburia","level":4,"score":0.47600969672203064},{"id":"https://openalex.org/C4725764","wikidata":"https://www.wikidata.org/wiki/Q844704","display_name":"Extraction (chemistry)","level":2,"score":0.45942309498786926},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.4490616023540497},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.2902275323867798},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.2633584439754486},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.24356406927108765},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.1899537742137909},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.14287620782852173},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.1330934464931488},{"id":"https://openalex.org/C2780797713","wikidata":"https://www.wikidata.org/wiki/Q625376","display_name":"Water quality","level":2,"score":0.0},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D000328","descriptor_name":"Adult","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001439","descriptor_name":"Bacteroides","qualifier_ui":"Q000145","qualifier_name":"classification","is_major_topic":false},{"descriptor_ui":"D001439","descriptor_name":"Bacteroides","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001644","descriptor_name":"Bifidobacterium","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D003013","descriptor_name":"Clostridium","qualifier_ui":"Q000145","qualifier_name":"classification","is_major_topic":true},{"descriptor_ui":"D003013","descriptor_name":"Clostridium","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004269","descriptor_name":"DNA, Bacterial","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D004587","descriptor_name":"Electrophoresis, Agar Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005051","descriptor_name":"Eubacterium","qualifier_ui":"Q000145","qualifier_name":"classification","is_major_topic":false},{"descriptor_ui":"D005051","descriptor_name":"Eubacterium","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D005243","descriptor_name":"Feces","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":true},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008875","descriptor_name":"Middle Aged","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015373","descriptor_name":"Bacterial Typing Techniques","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1111/j.1574-6941.2011.01257.x","doi":"https://doi.org/10.1111/j.1574-6941.2011.01257.x","is_oa":true,"landing_page_url":"https://doi.org/10.1111/j.1574-6941.2011.01257.x","pdf_url":"https://academic.oup.com/femsec/article-pdf/79/3/697/19525720/79-3-697.pdf","source":{"id":"https://openalex.org/S171282171","display_name":"FEMS Microbiology Ecology","issn_l":"0168-6496","issn":["0168-6496","1574-6941"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-c","jufo-1","ki-jl-1","medline","norway-2","oarl","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEMS Microbiology Ecology","raw_type":"journal-article"},{"id":"pmid:22098067","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/22098067","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEMS microbiology ecology","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1111/j.1574-6941.2011.01257.x","doi":"https://doi.org/10.1111/j.1574-6941.2011.01257.x","is_oa":true,"landing_page_url":"https://doi.org/10.1111/j.1574-6941.2011.01257.x","pdf_url":"https://academic.oup.com/femsec/article-pdf/79/3/697/19525720/79-3-697.pdf","source":{"id":"https://openalex.org/S171282171","display_name":"FEMS Microbiology Ecology","issn_l":"0168-6496","issn":["0168-6496","1574-6941"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-c","jufo-1","ki-jl-1","medline","norway-2","oarl","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEMS Microbiology Ecology","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.5683000087738037,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G2727781049","display_name":"Molecular Targets Open for Regulation by the gut flora \u2013 New Avenues for improved Diet to Optimize European health","funder_award_id":"222720","funder_id":"https://openalex.org/F4320320300","funder_display_name":"European Commission"}],"funders":[{"id":"https://openalex.org/F4320320300","display_name":"European Commission","ror":"https://ror.org/00k4n6c32"},{"id":"https://openalex.org/F4320321855","display_name":"Tekes","ror":"https://ror.org/02ag8cq23"},{"id":"https://openalex.org/F4320338366","display_name":"FP7 Food, Agriculture and Fisheries, Biotechnology","ror":null}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2147494764.pdf","grobid_xml":"https://content.openalex.org/works/W2147494764.grobid-xml"},"referenced_works_count":50,"referenced_works":["https://openalex.org/W1799266252","https://openalex.org/W1853321481","https://openalex.org/W1891948122","https://openalex.org/W1932402193","https://openalex.org/W1969466197","https://openalex.org/W1971415551","https://openalex.org/W1977763009","https://openalex.org/W1980003108","https://openalex.org/W2012854889","https://openalex.org/W2014216380","https://openalex.org/W2014766007","https://openalex.org/W2015274737","https://openalex.org/W2022837689","https://openalex.org/W2030091490","https://openalex.org/W2030674374","https://openalex.org/W2032230795","https://openalex.org/W2037701117","https://openalex.org/W2047118920","https://openalex.org/W2047703946","https://openalex.org/W2052969143","https://openalex.org/W2055043387","https://openalex.org/W2061016076","https://openalex.org/W2064642161","https://openalex.org/W2073332363","https://openalex.org/W2084793748","https://openalex.org/W2090148767","https://openalex.org/W2094244872","https://openalex.org/W2116531370","https://openalex.org/W2119761326","https://openalex.org/W2120156188","https://openalex.org/W2126579635","https://openalex.org/W2131291912","https://openalex.org/W2131902769","https://openalex.org/W2134416902","https://openalex.org/W2136500114","https://openalex.org/W2138084574","https://openalex.org/W2138253380","https://openalex.org/W2139784934","https://openalex.org/W2142967049","https://openalex.org/W2144826002","https://openalex.org/W2145336165","https://openalex.org/W2149243422","https://openalex.org/W2149573313","https://openalex.org/W2152885278","https://openalex.org/W2155066423","https://openalex.org/W2165924947","https://openalex.org/W2167062509","https://openalex.org/W2169330513","https://openalex.org/W2406391164","https://openalex.org/W4285719527"],"related_works":["https://openalex.org/W1488018780","https://openalex.org/W2012854889","https://openalex.org/W4416282692","https://openalex.org/W4282960166","https://openalex.org/W2904078215","https://openalex.org/W2956219854","https://openalex.org/W4391909866","https://openalex.org/W4392756796","https://openalex.org/W3005516189","https://openalex.org/W1998966952"],"abstract_inverted_index":{"Recently":[0],"several":[1],"human":[2,38,172],"health-related":[3],"microbiota":[4],"studies":[5],"have":[6],"had":[7],"partly":[8],"contradictory":[9,204],"results.":[10],"As":[11,175],"some":[12],"differences":[13],"may":[14,201],"be":[15],"explained":[16],"by":[17],"methodologies":[18],"applied,":[19,194],"we":[20],"evaluated":[21],"how":[22],"different":[23,122,198],"storage":[24,62],"conditions":[25],"and":[26,35,92,137,153,169,184],"commonly":[27],"used":[28],"DNA-extraction":[29,46,105,192,199],"kits":[30],"affect":[31,49],"bacterial":[32,115,167],"composition,":[33,52],"diversity,":[34,51],"numbers":[36,67,81,168],"of":[37,55,68,82,111,117,135,165,182,197],"fecal":[39,173],"microbiota.":[40],"According":[41,156],"to":[42,157],"our":[43,158],"results,":[44,159],"the":[45,50,66,80,114,127,144,180,191,195,203],"did":[47],"not":[48],"or":[53],"quantity":[54],"Bacteroides":[56,69],"spp.,":[57],"whereas":[58,140],"after":[59,103,121,124,141],"a":[60],"week's":[61],"at":[63],"-20":[64],"\u00b0C,":[65],"spp.":[70],"were":[71,95,132,148],"1.6-2.5":[72],"log":[73,97],"units":[74,98],"lower":[75],"(P":[76,100],"<":[77,101],"0.05).":[78],"Furthermore,":[79,113],"predominant":[83],"bacteria,":[84],"Eubacterium":[85],"rectale":[86],"(Erec)-group,":[87],"Clostridium":[88],"leptum":[89],"group,":[90],"bifidobacteria,":[91],"Atopobium":[93],"group":[94],"0.5-4":[96],"higher":[99,166],"0.05)":[102],"mechanical":[104,142,161],"as":[106],"detected":[107,131],"with":[108],"qPCR,":[109],"regardless":[110],"storage.":[112],"composition":[116],"Erec-group":[118],"differed":[119],"significantly":[120],"DNA-extractions;":[123],"enzymatic":[125],"DNA-extraction,":[126,143],"most":[128,145],"prevalent":[129,146],"genera":[130,147],"Roseburia":[133],"(39%":[134],"clones)":[136],"Coprococcus":[138,151],"(10%),":[139],"Blautia":[149],"(30%),":[150],"(13%),":[152],"Dorea":[154],"(10%).":[155],"rigorous":[160],"lysis":[162],"enables":[163],"detection":[164],"diversity":[170],"from":[171],"samples.":[174],"it":[176],"was":[177],"shown":[178],"that":[179],"results":[181,205],"clostridial":[183],"actinobacterial":[185],"populations":[186],"are":[187],"highly":[188],"dependent":[189],"on":[190],"methods":[193],"use":[196],"protocols":[200],"explain":[202],"previously":[206],"obtained.":[207]},"counts_by_year":[{"year":2026,"cited_by_count":3},{"year":2025,"cited_by_count":4},{"year":2024,"cited_by_count":4},{"year":2023,"cited_by_count":10},{"year":2022,"cited_by_count":5},{"year":2021,"cited_by_count":6},{"year":2020,"cited_by_count":10},{"year":2019,"cited_by_count":7},{"year":2018,"cited_by_count":5},{"year":2017,"cited_by_count":10},{"year":2016,"cited_by_count":13},{"year":2015,"cited_by_count":13},{"year":2014,"cited_by_count":20},{"year":2013,"cited_by_count":12},{"year":2012,"cited_by_count":4}],"updated_date":"2026-11-12T12:28:57.059296","created_date":"2016-06-24T00:00:00"}