{"id":"https://openalex.org/W2146909181","doi":"https://doi.org/10.1128/aem.00186-08","title":"Adaptive Evolution of a Lactose-Consuming Saccharomyces cerevisiae Recombinant","display_name":"Adaptive Evolution of a Lactose-Consuming Saccharomyces cerevisiae Recombinant","publication_year":2008,"publication_date":"2008-02-02","ids":{"openalex":"https://openalex.org/W2146909181","doi":"https://doi.org/10.1128/aem.00186-08","mag":"2146909181","pmid":"https://pubmed.ncbi.nlm.nih.gov/18245248","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/2268319"},"language":"en","primary_location":{"id":"doi:10.1128/aem.00186-08","doi":"https://doi.org/10.1128/aem.00186-08","is_oa":false,"landing_page_url":"https://doi.org/10.1128/aem.00186-08","pdf_url":null,"source":{"id":"https://openalex.org/S158228368","display_name":"Applied and Environmental Microbiology","issn_l":"0099-2240","issn":["0099-2240","1070-6291","1098-5336"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied and Environmental Microbiology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2268319","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5007408777","display_name":"Pedro M. R. Guimar\u00e3es","orcid":"https://orcid.org/0000-0002-5366-1481","observed_orcids":["https://orcid.org/0000-0002-5366-1481"]},"institutions":[{"id":"https://openalex.org/I4210167387","display_name":"Institute for Biotechnology and Bioengineering","ror":"https://ror.org/05ws8g470","country_code":"PT","type":"facility","lineage":["https://openalex.org/I4210167387"]},{"id":"https://openalex.org/I99682543","display_name":"University of Minho","ror":"https://ror.org/037wpkx04","country_code":"PT","type":"education","lineage":["https://openalex.org/I99682543"]}],"countries":["PT"],"is_corresponding":false,"raw_author_name":"Pedro M. R. Guimara\u0303es","raw_affiliation_strings":["Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar 4710-057, Braga, Portugal"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar 4710-057, Braga, Portugal","institution_ids":["https://openalex.org/I4210167387","https://openalex.org/I99682543"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5037156035","display_name":"Jean Fran\u00e7ois","orcid":"https://orcid.org/0000-0001-9884-5535","observed_orcids":["https://orcid.org/0000-0001-9884-5535"]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I134560555","display_name":"Universit\u00e9 Toulouse III - Paul Sabatier","ror":"https://ror.org/02v6kpv12","country_code":"FR","type":"education","lineage":["https://openalex.org/I134560555","https://openalex.org/I4405258862"]},{"id":"https://openalex.org/I196454796","display_name":"Institut National des Sciences Appliqu\u00e9es de Toulouse","ror":"https://ror.org/01h8pf755","country_code":"FR","type":"education","lineage":["https://openalex.org/I196454796"]},{"id":"https://openalex.org/I4210110888","display_name":"Toulouse Biotechnology Institute, Bio & Chemical Engineering","ror":"https://ror.org/01yypjb30","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I196454796","https://openalex.org/I4210088668","https://openalex.org/I4210110888"]},{"id":"https://openalex.org/I4405258862","display_name":"Universit\u00e9 de Toulouse","ror":"https://ror.org/01ahyrz84","country_code":"FR","type":"education","lineage":["https://openalex.org/I4405258862"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Jean Franc\u0327ois","raw_affiliation_strings":["CNRS, UMR5504, F-31400 Toulouse, France","INRA, UMR792 Inge\u0301nierie des Syste\u0300mes Biologiques et des Proce\u0301de\u0301s, F-31400 Toulouse, France","Universite\u0301 de Toulouse, INSA, UPS, INP, LISBP, 135 Avenue de Rangueil, F-31077 Toulouse, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"CNRS, UMR5504, F-31400 Toulouse, France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I4210110888"]},{"raw_affiliation_string":"INRA, UMR792 Inge\u0301nierie des Syste\u0300mes Biologiques et des Proce\u0301de\u0301s, F-31400 Toulouse, France","institution_ids":["https://openalex.org/I4210110888"]},{"raw_affiliation_string":"Universite\u0301 de Toulouse, INSA, UPS, INP, LISBP, 135 Avenue de Rangueil, F-31077 Toulouse, France","institution_ids":["https://openalex.org/I134560555","https://openalex.org/I196454796","https://openalex.org/I4210110888","https://openalex.org/I4405258862"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5078884635","display_name":"Jean Luc Parrou","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I134560555","display_name":"Universit\u00e9 Toulouse III - Paul Sabatier","ror":"https://ror.org/02v6kpv12","country_code":"FR","type":"education","lineage":["https://openalex.org/I134560555","https://openalex.org/I4405258862"]},{"id":"https://openalex.org/I196454796","display_name":"Institut National des Sciences Appliqu\u00e9es de Toulouse","ror":"https://ror.org/01h8pf755","country_code":"FR","type":"education","lineage":["https://openalex.org/I196454796"]},{"id":"https://openalex.org/I4210110888","display_name":"Toulouse Biotechnology Institute, Bio & Chemical Engineering","ror":"https://ror.org/01yypjb30","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I196454796","https://openalex.org/I4210088668","https://openalex.org/I4210110888"]},{"id":"https://openalex.org/I4405258862","display_name":"Universit\u00e9 de Toulouse","ror":"https://ror.org/01ahyrz84","country_code":"FR","type":"education","lineage":["https://openalex.org/I4405258862"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Jean Luc Parrou","raw_affiliation_strings":["CNRS, UMR5504, F-31400 Toulouse, France","INRA, UMR792 Inge\u0301nierie des Syste\u0300mes Biologiques et des Proce\u0301de\u0301s, F-31400 Toulouse, France","Universite\u0301 de Toulouse, INSA, UPS, INP, LISBP, 135 Avenue de Rangueil, F-31077 Toulouse, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"CNRS, UMR5504, F-31400 Toulouse, France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I4210110888"]},{"raw_affiliation_string":"INRA, UMR792 Inge\u0301nierie des Syste\u0300mes Biologiques et des Proce\u0301de\u0301s, F-31400 Toulouse, France","institution_ids":["https://openalex.org/I4210110888"]},{"raw_affiliation_string":"Universite\u0301 de Toulouse, INSA, UPS, INP, LISBP, 135 Avenue de Rangueil, F-31077 Toulouse, France","institution_ids":["https://openalex.org/I134560555","https://openalex.org/I196454796","https://openalex.org/I4210110888","https://openalex.org/I4405258862"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5031274455","display_name":"Jos\u00e9 Ant\u00f3nio Teixeira","orcid":"https://orcid.org/0000-0002-4918-3704","observed_orcids":["https://orcid.org/0000-0002-4918-3704"]},"institutions":[{"id":"https://openalex.org/I4210167387","display_name":"Institute for Biotechnology and Bioengineering","ror":"https://ror.org/05ws8g470","country_code":"PT","type":"facility","lineage":["https://openalex.org/I4210167387"]},{"id":"https://openalex.org/I99682543","display_name":"University of Minho","ror":"https://ror.org/037wpkx04","country_code":"PT","type":"education","lineage":["https://openalex.org/I99682543"]}],"countries":["PT"],"is_corresponding":false,"raw_author_name":"Jose\u0301 A. Teixeira","raw_affiliation_strings":["Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar 4710-057, Braga, Portugal"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar 4710-057, Braga, Portugal","institution_ids":["https://openalex.org/I4210167387","https://openalex.org/I99682543"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5073891770","display_name":"Luc\u0131\u0301lia Domingues","orcid":"https://orcid.org/0000-0003-1089-7627","observed_orcids":["https://orcid.org/0000-0003-1089-7627"]},"institutions":[{"id":"https://openalex.org/I4210167387","display_name":"Institute for Biotechnology and Bioengineering","ror":"https://ror.org/05ws8g470","country_code":"PT","type":"facility","lineage":["https://openalex.org/I4210167387"]},{"id":"https://openalex.org/I99682543","display_name":"University of Minho","ror":"https://ror.org/037wpkx04","country_code":"PT","type":"education","lineage":["https://openalex.org/I99682543"]}],"countries":["PT"],"is_corresponding":false,"raw_author_name":"Luci\u0301lia Domingues","raw_affiliation_strings":["Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar 4710-057, Braga, Portugal"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar 4710-057, Braga, Portugal","institution_ids":["https://openalex.org/I4210167387","https://openalex.org/I99682543"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":7,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":2.912,"has_fulltext":false,"cited_by_count":97,"citation_normalized_percentile":{"value":0.92145904,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"74","issue":"6","first_page":"1748","last_page":"1756"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.5770999789237976,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.5770999789237976,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.4000999927520752,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10171","display_name":"Biofuel production and bioconversion","score":0.004800000227987766,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/saccharomyces-cerevisiae","display_name":"Saccharomyces cerevisiae","score":0.9509999752044678},{"id":"https://openalex.org/keywords/lactose-fermentation","display_name":"lactose fermentation","score":0.9829999804496765},{"id":"https://openalex.org/keywords/lactose-permease","display_name":"lactose permease","score":0.9010000228881836},{"id":"https://openalex.org/keywords/beta-galactosidase","display_name":"beta-galactosidase","score":0.9990000128746033},{"id":"https://openalex.org/keywords/heterologous-expression","display_name":"heterologous expression","score":0.8149999976158142},{"id":"https://openalex.org/keywords/promoter-deletion","display_name":"promoter deletion","score":0.7639999985694885},{"id":"https://openalex.org/keywords/plasmid-copy-number","display_name":"plasmid copy number","score":0.968999981880188},{"id":"https://openalex.org/keywords/adaptive-evolution","display_name":"adaptive evolution","score":0.6970000267028809},{"id":"https://openalex.org/keywords/ethanol-production","display_name":"ethanol production","score":0.7739999890327454}],"concepts":[{"id":"https://openalex.org/C2778724459","wikidata":"https://www.wikidata.org/wiki/Q127900","display_name":"Lactose","level":2,"score":0.8058138489723206},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.7558712959289551},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6649923324584961},{"id":"https://openalex.org/C2778041695","wikidata":"https://www.wikidata.org/wiki/Q6421540","display_name":"Kluyveromyces lactis","level":4,"score":0.6525757312774658},{"id":"https://openalex.org/C58598135","wikidata":"https://www.wikidata.org/wiki/Q24780812","display_name":"Lactose permease","level":5,"score":0.6475518941879272},{"id":"https://openalex.org/C2777576037","wikidata":"https://www.wikidata.org/wiki/Q719725","display_name":"Saccharomyces cerevisiae","level":3,"score":0.6276332139968872},{"id":"https://openalex.org/C170344550","wikidata":"https://www.wikidata.org/wiki/Q311359","display_name":"lac operon","level":4,"score":0.48364102840423584},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.4589020609855652},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.4240700304508209},{"id":"https://openalex.org/C2778022156","wikidata":"https://www.wikidata.org/wiki/Q576145","display_name":"Strain (injury)","level":2,"score":0.42364057898521423},{"id":"https://openalex.org/C100544194","wikidata":"https://www.wikidata.org/wiki/Q41760","display_name":"Fermentation","level":2,"score":0.4140198528766632},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.377327561378479},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.29034045338630676},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.16715294122695923},{"id":"https://openalex.org/C190114821","wikidata":"https://www.wikidata.org/wiki/Q412861","display_name":"Permease","level":4,"score":0.08340263366699219},{"id":"https://openalex.org/C105702510","wikidata":"https://www.wikidata.org/wiki/Q514","display_name":"Anatomy","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D001616","descriptor_name":"beta-Galactosidase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D001616","descriptor_name":"beta-Galactosidase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005285","descriptor_name":"Fermentation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005690","descriptor_name":"Galactose","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005947","descriptor_name":"Glucose","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D007716","descriptor_name":"Kluyveromyces","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D007716","descriptor_name":"Kluyveromyces","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D007785","descriptor_name":"Lactose","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011995","descriptor_name":"Recombination, Genetic","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D012441","descriptor_name":"Saccharomyces cerevisiae","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D012441","descriptor_name":"Saccharomyces cerevisiae","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019020","descriptor_name":"Directed Molecular Evolution","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020133","descriptor_name":"Reverse Transcriptase Polymerase Chain Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D026901","descriptor_name":"Membrane Transport Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D026901","descriptor_name":"Membrane Transport Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false}],"locations_count":8,"locations":[{"id":"doi:10.1128/aem.00186-08","doi":"https://doi.org/10.1128/aem.00186-08","is_oa":false,"landing_page_url":"https://doi.org/10.1128/aem.00186-08","pdf_url":null,"source":{"id":"https://openalex.org/S158228368","display_name":"Applied and Environmental Microbiology","issn_l":"0099-2240","issn":["0099-2240","1070-6291","1098-5336"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied and Environmental Microbiology","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:2268319","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2268319","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Appl Environ Microbiol","raw_type":"Text"},{"id":"pmid:18245248","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/18245248","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied and environmental microbiology","raw_type":"Journal Article"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.321.5642","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.321.5642","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://aem.asm.org/content/74/6/1748.full.pdf","raw_type":"text"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.564.7006","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.564.7006","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://repositorium.sdum.uminho.pt/bitstream/1822/7975/1/Guimaraes1_AEM[1].pdf","raw_type":"text"},{"id":"pmh:oai:HAL:hal-02559846v1","doi":null,"is_oa":true,"landing_page_url":"https://insa-toulouse.hal.science/hal-02559846","pdf_url":null,"source":{"id":"https://openalex.org/S4306402512","display_name":"HAL","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4405252976","host_organization_name":"Centre pour la Communication Scientifique Directe","host_organization_lineage":["https://openalex.org/I4405252976"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Applied and Environmental Microbiology, 2008, 74 (6), pp.1748-1756. ⟨10.1128/AEM.00186-08⟩","raw_type":"info:eu-repo/semantics/article"},{"id":"pmh:oai:repositorium.sdum.uminho.pt:1822/7463","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/1822/7463","pdf_url":null,"source":{"id":"https://openalex.org/S4306402433","display_name":"Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"conference object"},{"id":"pmh:oai:repositorium.sdum.uminho.pt:1822/7975","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/1822/7975","pdf_url":null,"source":{"id":"https://openalex.org/S4306402433","display_name":"Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"journal article"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:2268319","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2268319","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Appl Environ Microbiol","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320320883","display_name":"Agence Nationale de la Recherche","ror":"https://ror.org/00rbzpz17"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":49,"referenced_works":["https://openalex.org/W92313992","https://openalex.org/W1513717105","https://openalex.org/W1551957277","https://openalex.org/W1763161027","https://openalex.org/W1764011743","https://openalex.org/W1904257689","https://openalex.org/W1908493956","https://openalex.org/W1908662736","https://openalex.org/W1948213703","https://openalex.org/W1968604794","https://openalex.org/W1980629935","https://openalex.org/W1990198788","https://openalex.org/W1990212260","https://openalex.org/W2004962297","https://openalex.org/W2005377356","https://openalex.org/W2026066874","https://openalex.org/W2030728458","https://openalex.org/W2035397505","https://openalex.org/W2043693423","https://openalex.org/W2047597963","https://openalex.org/W2053837262","https://openalex.org/W2060770770","https://openalex.org/W2073701512","https://openalex.org/W2082579794","https://openalex.org/W2086660631","https://openalex.org/W2101442503","https://openalex.org/W2102276577","https://openalex.org/W2110821951","https://openalex.org/W2115470699","https://openalex.org/W2116734717","https://openalex.org/W2120272121","https://openalex.org/W2120644786","https://openalex.org/W2121078941","https://openalex.org/W2121781334","https://openalex.org/W2122559587","https://openalex.org/W2128635872","https://openalex.org/W2129134180","https://openalex.org/W2131921511","https://openalex.org/W2135908280","https://openalex.org/W2137441463","https://openalex.org/W2143376835","https://openalex.org/W2144634347","https://openalex.org/W2148634741","https://openalex.org/W2149842777","https://openalex.org/W2162635255","https://openalex.org/W2163949203","https://openalex.org/W2169142563","https://openalex.org/W2989345970","https://openalex.org/W4247892585"],"related_works":["https://openalex.org/W7097143001","https://openalex.org/W2257047657","https://openalex.org/W2172060270","https://openalex.org/W4283368348","https://openalex.org/W1997467072","https://openalex.org/W2026066874","https://openalex.org/W1990198788","https://openalex.org/W2222472523","https://openalex.org/W53847938","https://openalex.org/W7095742238"],"abstract_inverted_index":{"The":[0,125,245],"construction":[1],"of":[2,32,110,138,141,159,171,174,193,200,237,260],"Saccharomyces":[3],"cerevisiae":[4,21],"strains":[5],"that":[6,68,86,120,128,152,168,222],"ferment":[7],"lactose":[8,43,60,70,146,229],"has":[9],"biotechnological":[10],"interest,":[11],"particularly":[12],"for":[13,203,257],"cheese":[14,251],"whey":[15],"fermentation.":[16,44],"A":[17],"flocculent":[18],"lactose-consuming":[19],"S.":[20],"recombinant":[22,66,150,182],"expressing":[23],"the":[24,78,88,92,99,111,122,129,136,139,148,153,156,163,169,172,175,180,186,189,197,209,227,235,258],"LAC12":[25,106,144,206],"(lactose":[26],"permease)":[27],"and":[28,57,105,107,143,151,196,205],"LAC4":[29,104,142,204],"(beta-galactosidase)":[30],"genes":[31,161,178,195],"Kluyveromyces":[33],"lactis":[34],"was":[35,47,132,183],"constructed":[36],"previously":[37],"but":[38],"showed":[39],"poor":[40],"efficiency":[41],"in":[42,59,91,98,121,147,162,179,242],"This":[45,232],"strain":[46,67,243,247],"therefore":[48],"subjected":[49],"to":[50,119,134,226],"an":[51,64,254],"evolutionary":[52,239],"engineering":[53,240],"process":[54],"(serial":[55],"transfer":[56],"dilution":[58],"medium),":[61],"which":[62],"yielded":[63],"evolved":[65,93,164,181,246],"consumed":[69],"twofold":[71],"faster,":[72],"producing":[73],"30%":[74],"more":[75],"ethanol":[76],"than":[77],"original":[79,123,149],"recombinant.":[80,124],"We":[81,166],"identified":[82],"two":[83],"molecular":[84],"events":[85],"targeted":[87],"LAC":[89,177],"construct":[90],"strain:":[94],"a":[95,108],"1,593-bp":[96],"deletion":[97,154],"intergenic":[100],"region":[101],"(promoter)":[102],"between":[103,188],"decrease":[109],"plasmid":[112],"copy":[113,191],"number":[114,192],"by":[115,145,185],"about":[116],"10-fold":[117],"compared":[118],"results":[126,215],"suggest":[127],"intact":[130],"promoter":[131,211],"unable":[133],"mediate":[135],"induction":[137,158,202],"transcription":[140],"established":[155],"transcriptional":[157,201],"both":[160,194],"strain.":[165],"propose":[167],"tuning":[170],"expression":[173],"heterologous":[176],"accomplished":[184],"interplay":[187],"decreased":[190],"different":[198],"levels":[199],"resulting":[207],"from":[208],"changed":[210],"structure.":[212],"Nevertheless,":[213],"our":[214],"do":[216],"not":[217],"exclude":[218],"other":[219],"possible":[220],"mutations":[221],"may":[223],"have":[224],"contributed":[225],"improved":[228],"fermentation":[230,259],"phenotype.":[231],"study":[233],"illustrates":[234],"usefulness":[236],"simple":[238],"approaches":[241],"improvement.":[244],"efficiently":[248],"fermented":[249],"threefold-concentrated":[250],"whey,":[252],"providing":[253],"attractive":[255],"alternative":[256],"lactose-based":[261],"media.":[262]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":3},{"year":2022,"cited_by_count":4},{"year":2021,"cited_by_count":6},{"year":2020,"cited_by_count":4},{"year":2019,"cited_by_count":4},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":7},{"year":2016,"cited_by_count":9},{"year":2015,"cited_by_count":5},{"year":2014,"cited_by_count":6},{"year":2013,"cited_by_count":7},{"year":2012,"cited_by_count":12}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}