{"id":"https://openalex.org/W2145197472","doi":null,"title":"A phage display vector optimized for the generation of human antibody combinatorial libraries and the molecular cloning of monoclonal antibody fragments.","display_name":"A phage display vector optimized for the generation of human antibody combinatorial libraries and the molecular cloning of monoclonal antibody fragments.","publication_year":2012,"publication_date":"2012-07-01","ids":{"openalex":"https://openalex.org/W2145197472","mag":"2145197472","pmid":"https://pubmed.ncbi.nlm.nih.gov/22842598"},"language":"en","primary_location":{"id":"pmid:22842598","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/22842598","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The new microbiologica","raw_type":"Journal Article"},"volume":null,"type":"article","indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5055407583","display_name":"Laura Solforosi","orcid":"https://orcid.org/0000-0003-3786-6468","observed_orcids":["https://orcid.org/0000-0003-3786-6468"]},"institutions":[{"id":"https://openalex.org/I154387261","display_name":"Vita-Salute San Raffaele University","ror":"https://ror.org/01gmqr298","country_code":"IT","type":"education","lineage":["https://openalex.org/I154387261"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Laura Solforosi","raw_affiliation_strings":["Laboratory of Microbiology and Virology, Vita-Salute San Raffaele University, Milan, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Microbiology and Virology, Vita-Salute San Raffaele University, Milan, Italy","institution_ids":["https://openalex.org/I154387261"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5029436501","display_name":"Nicasio Mancini","orcid":"https://orcid.org/0000-0003-0637-0910","observed_orcids":["https://orcid.org/0000-0003-0637-0910"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Nicasio Mancini","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5062018498","display_name":"Filippo Canducci","orcid":"https://orcid.org/0000-0002-5637-6205","observed_orcids":["https://orcid.org/0000-0002-5637-6205"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Filippo Canducci","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5008282651","display_name":"Nicola Clementi","orcid":"https://orcid.org/0000-0002-1822-9861","observed_orcids":["https://orcid.org/0000-0002-1822-9861"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Nicola Clementi","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5028853494","display_name":"Giuseppe Andrea Sautto","orcid":"https://orcid.org/0000-0002-0273-2235","observed_orcids":["https://orcid.org/0000-0002-0273-2235"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Giuseppe Andrea Sautto","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5057292276","display_name":"Roberta A. Diotti","orcid":"https://orcid.org/0000-0002-7853-3224","observed_orcids":["https://orcid.org/0000-0002-7853-3224"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Roberta Antonia Diotti","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5004847381","display_name":"Massimo Clementi","orcid":"https://orcid.org/0000-0002-7461-5898","observed_orcids":["https://orcid.org/0000-0002-7461-5898"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Massimo Clementi","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5047644781","display_name":"Roberto Burioni","orcid":"https://orcid.org/0000-0001-6760-9898","observed_orcids":["https://orcid.org/0000-0001-6760-9898"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Roberto Burioni","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":3.6208,"has_fulltext":false,"cited_by_count":32,"citation_normalized_percentile":{"value":0.92412938,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"35","issue":"3","first_page":"289","last_page":"94"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/2741","display_name":"Radiology, Nuclear Medicine and Imaging"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/2741","display_name":"Radiology, Nuclear Medicine and Imaging"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11048","display_name":"Bacteriophages and microbial interactions","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/filamentous-phage","display_name":"filamentous phage","score":0.9120000004768372},{"id":"https://openalex.org/keywords/monoclonal-antibody-fragments","display_name":"monoclonal antibody fragments","score":0.8460000157356262},{"id":"https://openalex.org/keywords/lacz-promoter","display_name":"lacZ promoter","score":0.8349999785423279},{"id":"https://openalex.org/keywords/pelb-signal-peptide","display_name":"pelB signal peptide","score":0.968999981880188},{"id":"https://openalex.org/keywords/antibody-selection","display_name":"antibody selection","score":0.4519999921321869}],"concepts":[{"id":"https://openalex.org/C10079403","wikidata":"https://www.wikidata.org/wiki/Q424475","display_name":"Phagemid","level":5,"score":0.9131610989570618},{"id":"https://openalex.org/C186268636","wikidata":"https://www.wikidata.org/wiki/Q896217","display_name":"Phage display","level":3,"score":0.7145930528640747},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.6218127608299255},{"id":"https://openalex.org/C36394416","wikidata":"https://www.wikidata.org/wiki/Q949267","display_name":"Immunoglobulin light chain","level":3,"score":0.6198140382766724},{"id":"https://openalex.org/C107618985","wikidata":"https://www.wikidata.org/wiki/Q358963","display_name":"Multiple cloning site","level":5,"score":0.562998354434967},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5192891955375671},{"id":"https://openalex.org/C121050878","wikidata":"https://www.wikidata.org/wiki/Q5135020","display_name":"Cloning (programming)","level":2,"score":0.48923707008361816},{"id":"https://openalex.org/C542903549","wikidata":"https://www.wikidata.org/wiki/Q422248","display_name":"Monoclonal antibody","level":3,"score":0.47083142399787903},{"id":"https://openalex.org/C19924922","wikidata":"https://www.wikidata.org/wiki/Q408351","display_name":"Molecular cloning","level":4,"score":0.46732062101364136},{"id":"https://openalex.org/C187882448","wikidata":"https://www.wikidata.org/wiki/Q283478","display_name":"Complementary DNA","level":3,"score":0.4555432200431824},{"id":"https://openalex.org/C13575015","wikidata":"https://www.wikidata.org/wiki/Q1562492","display_name":"Genomic library","level":4,"score":0.4183872938156128},{"id":"https://openalex.org/C168374021","wikidata":"https://www.wikidata.org/wiki/Q1924179","display_name":"Cloning vector","level":5,"score":0.4121183753013611},{"id":"https://openalex.org/C2776441376","wikidata":"https://www.wikidata.org/wiki/Q165028","display_name":"Bacteriophage","level":4,"score":0.40410205721855164},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3844178020954132},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.3794066905975342},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.355949342250824},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.3134692311286926},{"id":"https://openalex.org/C33161422","wikidata":"https://www.wikidata.org/wiki/Q5421712","display_name":"Expression vector","level":4,"score":0.29599249362945557},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.257119357585907},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.25471097230911255},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.16624456644058228},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.0},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D005822","descriptor_name":"Genetic Vectors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007140","descriptor_name":"Immunoglobulin Fab Fragments","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D007140","descriptor_name":"Immunoglobulin Fab Fragments","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D007763","descriptor_name":"Lac Operon","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008875","descriptor_name":"Middle Aged","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011401","descriptor_name":"Promoter Regions, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D014758","descriptor_name":"Viral Core Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D016601","descriptor_name":"RNA-Binding Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D018076","descriptor_name":"DNA, Complementary","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D019151","descriptor_name":"Peptide Library","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D019590","descriptor_name":"Nucleocapsid Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D021382","descriptor_name":"Protein Sorting Signals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D053118","descriptor_name":"Influenza A Virus, H1N1 Subtype","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false}],"locations_count":4,"locations":[{"id":"pmid:22842598","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/22842598","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The new microbiologica","raw_type":"Journal Article"},{"id":"pmh:oai:irinsubria.uninsubria.it:11383/1879924","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/11383/1879924","pdf_url":null,"source":{"id":"https://openalex.org/S4377196351","display_name":"IrInSubria (University of Insubria)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I115752224","host_organization_name":"University of Insubria","host_organization_lineage":["https://openalex.org/I115752224"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"info:eu-repo/semantics/article"},{"id":"pmh:oai:iris.unisr.it:20.500.11768/47183","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/20.500.11768/47183","pdf_url":null,"source":{"id":"https://openalex.org/S7407055348","display_name":"Archivio Istituzionale della Ricerca - Universit\u00e0 Vita-Salute San Raffaele","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"info:eu-repo/semantics/article"},{"id":"mag:2145197472","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/22842598","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.4000000059604645}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":35,"referenced_works":["https://openalex.org/W1517594500","https://openalex.org/W1669046622","https://openalex.org/W1962721184","https://openalex.org/W1991544402","https://openalex.org/W1992745490","https://openalex.org/W1993371307","https://openalex.org/W2000534965","https://openalex.org/W2003215618","https://openalex.org/W2003537304","https://openalex.org/W2006311979","https://openalex.org/W2009329755","https://openalex.org/W2009857746","https://openalex.org/W2016281153","https://openalex.org/W2033891839","https://openalex.org/W2040432489","https://openalex.org/W2048428214","https://openalex.org/W2050860711","https://openalex.org/W2057251299","https://openalex.org/W2061530347","https://openalex.org/W2063092703","https://openalex.org/W2071378581","https://openalex.org/W2071633990","https://openalex.org/W2099046838","https://openalex.org/W2102331928","https://openalex.org/W2103904572","https://openalex.org/W2105364445","https://openalex.org/W2107750870","https://openalex.org/W2115289558","https://openalex.org/W2140557969","https://openalex.org/W2142230632","https://openalex.org/W2146900710","https://openalex.org/W2152111584","https://openalex.org/W2153019080","https://openalex.org/W2153686676","https://openalex.org/W2420424249"],"related_works":["https://openalex.org/W2016281153","https://openalex.org/W4249575653","https://openalex.org/W2273652869","https://openalex.org/W4403418243","https://openalex.org/W2353936914","https://openalex.org/W2063168718","https://openalex.org/W2146900710","https://openalex.org/W24320370","https://openalex.org/W1998945428","https://openalex.org/W2071378581"],"abstract_inverted_index":{"A":[0,154],"novel":[1],"phagemid":[2,73,124],"vector,":[3],"named":[4],"pCM,":[5],"was":[6,126],"optimized":[7],"for":[8,32],"the":[9,19,36,41,46,49,63,68,72,75,103,106,111,119,139,149],"cloning":[10,51],"and":[11,87],"display":[12,134],"of":[13,21,35,40,62,71,83,141,152],"antibody":[14,135,146],"fragment":[15,47,77,145],"(Fab)":[16],"libraries":[17],"on":[18],"surface":[20],"filamentous":[22],"phage.":[23],"This":[24],"vector":[25],"contains":[26,53],"two":[27],"long":[28],"DNA":[29],"\"stuffer\"":[30],"fragments":[31],"easier":[33],"differentiation":[34],"correctly":[37],"cut":[38],"forms":[39],"vector.":[42],"Moreover,":[43],"in":[44],"pCM":[45,81,125],"at":[48],"heavy-chain":[50,76,84,112],"site":[52],"an":[54,59],"acid":[55],"phosphatase-encoding":[56],"gene":[57],"allowing":[58],"easy":[60],"distinction":[61],"Escherichia":[64],"coli":[65],"cells":[66],"containing":[67],"unmodified":[69],"form":[70],"versus":[74],"coding":[78],"cDNA.":[79],"In":[80],"transcription":[82],"Fd/gene":[85],"III":[86,115],"light":[88,98],"chain":[89,99],"is":[90,100,116],"driven":[91],"by":[92,105,118],"a":[93,130,142],"single":[94],"lacZ":[95],"promoter.":[96],"The":[97,123],"directed":[101,147],"to":[102,128],"periplasm":[104],"ompA":[107],"signal":[108,121],"peptide,":[109],"whereas":[110],"Fd/coat":[113],"protein":[114],"trafficked":[117],"pelB":[120],"peptide.":[122],"used":[127],"generate":[129],"human":[131],"combinatorial":[132],"phage":[133],"library":[136],"that":[137],"allowed":[138],"selection":[140],"monoclonal":[143],"Fab":[144],"against":[148],"nucleoprotein":[150],"(NP)":[151],"Influenza":[153],"virus.":[155]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":4},{"year":2017,"cited_by_count":3},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":5},{"year":2012,"cited_by_count":6}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}
