{"id":"https://openalex.org/W2144679405","doi":null,"title":"Transfection of constitutively active mitogen-activated protein/extracellular signal-regulated kinase kinase confers tumorigenic and metastatic potentials to NIH3T3 cells.","display_name":"Transfection of constitutively active mitogen-activated protein/extracellular signal-regulated kinase kinase confers tumorigenic and metastatic potentials to NIH3T3 cells.","publication_year":2000,"publication_date":"2000-03-15","ids":{"openalex":"https://openalex.org/W2144679405","mag":"2144679405","pmid":"https://pubmed.ncbi.nlm.nih.gov/10749122"},"language":"en","primary_location":{"id":"pmid:10749122","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10749122","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer research","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5056217370","display_name":"Danny R. Welch","orcid":"https://orcid.org/0000-0002-1951-4947","observed_orcids":["https://orcid.org/0000-0002-1951-4947"]},"institutions":[{"id":"https://openalex.org/I130769515","display_name":"Pennsylvania State University","ror":"https://ror.org/04p491231","country_code":"US","type":"education","lineage":["https://openalex.org/I130769515"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"D R Welch","raw_affiliation_strings":["Jake Gittlen Cancer Research Institute, The Pennsylvania State University College of Medicine, Hershey 17033-2390, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Jake Gittlen Cancer Research Institute, The Pennsylvania State University College of Medicine, Hershey 17033-2390, USA","institution_ids":["https://openalex.org/I130769515"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109175873","display_name":"T Sakamaki","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"T Sakamaki","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5076492178","display_name":"R Pioquinto","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"R Pioquinto","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5025797084","display_name":"Timothy O. Leonard","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"T O Leonard","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5020517488","display_name":"Steven F. Goldberg","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"S F Goldberg","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5038183879","display_name":"Q Hon","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Q Hon","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5079289641","display_name":"R L Erikson","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"R L Erikson","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110270850","display_name":"MARY STRASBERG RIEBER","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"M Rieber","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110270850","display_name":"MARY STRASBERG RIEBER","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"M S Rieber","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103419491","display_name":"Diane J. Hicks","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"D J Hicks","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5089781525","display_name":"Joseph Vincent Bonventre","orcid":"https://orcid.org/0000-0001-7144-386X","observed_orcids":["https://orcid.org/0000-0001-7144-386X"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"J V Bonventre","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5103274674","display_name":"Alessandro Alessandrini","orcid":"https://orcid.org/0000-0003-0347-1927","observed_orcids":["https://orcid.org/0000-0003-0347-1927"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"A Alessandrini","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":1.5925,"has_fulltext":false,"cited_by_count":67,"citation_normalized_percentile":{"value":0.82586073,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":97},"biblio":{"volume":"60","issue":"6","first_page":"1552","last_page":"6"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11533","display_name":"Melanoma and MAPK Pathways","score":0.5717999935150146,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11533","display_name":"Melanoma and MAPK Pathways","score":0.5717999935150146,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.41190001368522644,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12104","display_name":"Protein Tyrosine Phosphatases","score":0.00419999985024333,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/mek1","display_name":"MEK1","score":0.9819999933242798},{"id":"https://openalex.org/keywords/oncogenic-transformation","display_name":"oncogenic transformation","score":0.8529999852180481},{"id":"https://openalex.org/keywords/tumorigenicity","display_name":"tumorigenicity","score":0.7990000247955322},{"id":"https://openalex.org/keywords/metastasis","display_name":"metastasis","score":0.9800000190734863},{"id":"https://openalex.org/keywords/nih-3t3-cells","display_name":"NIH3T3 cells","score":0.9079999923706055},{"id":"https://openalex.org/keywords/cathepsin-l","display_name":"cathepsin L","score":1.0},{"id":"https://openalex.org/keywords/ras","display_name":"Ras","score":0.33899998664855957},{"id":"https://openalex.org/keywords/raf","display_name":"Raf","score":0.33000001311302185},{"id":"https://openalex.org/keywords/nude-mouse-xenograft","display_name":"nude mouse xenograft","score":0.6449999809265137}],"concepts":[{"id":"https://openalex.org/C57074206","wikidata":"https://www.wikidata.org/wiki/Q14197552","display_name":"MAPK/ERK pathway","level":3,"score":0.7349087595939636},{"id":"https://openalex.org/C184235292","wikidata":"https://www.wikidata.org/wiki/Q421851","display_name":"Kinase","level":2,"score":0.6770168542861938},{"id":"https://openalex.org/C132149769","wikidata":"https://www.wikidata.org/wiki/Q899651","display_name":"Mitogen-activated protein kinase","level":3,"score":0.5606454610824585},{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.5525533556938171},{"id":"https://openalex.org/C97029542","wikidata":"https://www.wikidata.org/wiki/Q281417","display_name":"Protein kinase A","level":3,"score":0.5473905801773071},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.5470046997070312},{"id":"https://openalex.org/C2909504400","wikidata":"https://www.wikidata.org/wiki/Q409325","display_name":"Mitogen-Activated Protein Kinase 3","level":4,"score":0.5303341150283813},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.49111124873161316},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.46704500913619995},{"id":"https://openalex.org/C2776746612","wikidata":"https://www.wikidata.org/wiki/Q68153243","display_name":"3T3 cells","level":4,"score":0.43725132942199707},{"id":"https://openalex.org/C62478195","wikidata":"https://www.wikidata.org/wiki/Q828130","display_name":"Signal transduction","level":2,"score":0.43676304817199707},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.35954511165618896},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.32432883977890015},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.3095780909061432},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.04147493839263916}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002448","descriptor_name":"Cell Adhesion","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002461","descriptor_name":"Cell Line, Transformed","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002471","descriptor_name":"Cell Transformation, Neoplastic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D002999","descriptor_name":"Clone Cells","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008175","descriptor_name":"Lung Neoplasms","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D008175","descriptor_name":"Lung Neoplasms","qualifier_ui":"Q000556","qualifier_name":"secondary","is_major_topic":false},{"descriptor_ui":"D008819","descriptor_name":"Mice, Nude","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009362","descriptor_name":"Neoplasm Metastasis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009374","descriptor_name":"Neoplasms, Experimental","qualifier_ui":"Q000209","qualifier_name":"etiology","is_major_topic":false},{"descriptor_ui":"D009374","descriptor_name":"Neoplasms, Experimental","qualifier_ui":"Q000473","qualifier_name":"pathology","is_major_topic":false},{"descriptor_ui":"D014162","descriptor_name":"Transfection","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016475","descriptor_name":"3T3 Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017346","descriptor_name":"Protein Serine-Threonine Kinases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D020778","descriptor_name":"Matrix Metalloproteinase 2","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D020780","descriptor_name":"Matrix Metalloproteinase 9","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D020928","descriptor_name":"Mitogen-Activated Protein Kinases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D020928","descriptor_name":"Mitogen-Activated Protein Kinases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D020929","descriptor_name":"Mitogen-Activated Protein Kinase Kinases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D048369","descriptor_name":"MAP Kinase Kinase 1","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"pmid:10749122","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10749122","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer research","raw_type":"Journal Article"},{"id":"mag:2144679405","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10749122","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.8048999905586243}],"study_designs":[],"awards":[{"id":"https://openalex.org/G448396837","display_name":"METASTASIS SUPPRESSOR GENE IN HUMAN CUTANEOUS MELANOMA","funder_award_id":"R01CA062168","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"}],"funders":[{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":13,"referenced_works":["https://openalex.org/W14898210","https://openalex.org/W1232839958","https://openalex.org/W1493394907","https://openalex.org/W1505183758","https://openalex.org/W1556471675","https://openalex.org/W1976858755","https://openalex.org/W1991321737","https://openalex.org/W1993163893","https://openalex.org/W2023934008","https://openalex.org/W2069356042","https://openalex.org/W2090380700","https://openalex.org/W2104692426","https://openalex.org/W2147570487"],"related_works":["https://openalex.org/W2090380700","https://openalex.org/W2001521752","https://openalex.org/W2013112408","https://openalex.org/W1991321737","https://openalex.org/W1644484251","https://openalex.org/W2066378394","https://openalex.org/W2401237552","https://openalex.org/W313138826","https://openalex.org/W2055088010","https://openalex.org/W2068223140"],"abstract_inverted_index":{"Cellular":[0],"growth":[1,127],"and":[2,57,81,97,125,146,148,206,223,232],"differentiation":[3],"are":[4,235],"controlled":[5],"by":[6,63,77,83],"multiple":[7],"extracellular":[8,14],"signals,":[9],"many":[10],"of":[11,22,38,49,54,66,86,109,116,132,142,230,237],"which":[12,218],"activate":[13],"signal-regulated":[15],"kinase":[16,25],"(ERK)/mitogen-activated":[17],"protein":[18],"(MAP)":[19],"kinases.":[20],"Components":[21],"the":[23,60,157,166,215,227],"MAP":[24,50],"pathways":[26],"also":[27],"cause":[28],"oncogenic":[29],"transformation":[30,124],"in":[31,59,101,156],"their":[32],"constitutively":[33,102],"active":[34,103],"forms.":[35],"Moreover,":[36],"expression":[37,141],"activated":[39,76,199],"ras":[40,219],"can":[41,202],"confer":[42,203,221],"metastatic":[43,158,172,207,224,233],"potential":[44,173,208,225,234],"upon":[45,209],"some":[46],"cells.":[47,211],"Activation":[48],"kinases":[51],"requires":[52],"phosphorylation":[53,78],"both":[55,204],"Thr":[56],"Tyr":[58],"catalytic":[61],"domain":[62],"a":[64],"family":[65],"dual-specificity":[67],"kinases,":[68],"called":[69],"MEKs":[70],"(MAP":[71],"kinase/ERK":[72],"kinase).":[73],"MEK1":[74,191,201],"is":[75],"at":[79],"Ser218":[80],"Ser222":[82],"Raf.":[84],"Mutation":[85],"these":[87,106],"two":[88],"sites":[89],"to":[90],"acidic":[91],"residues,":[92],"specifically":[93],"[Asp218],":[94],"[Asp218,":[95,169],"Asp222],":[96],"[Glu218,":[98],"Glu222],":[99],"results":[100,213],"MEK1.":[104],"Using":[105],"mutant":[107,192],"variants":[108],"MEK1,":[110],"we":[111],"showed":[112],"previously":[113],"that":[114,152],"transfection":[115],"NIH/3T3":[117],"or":[118,168,187],"Swiss":[119],"3T3":[120,181],"cells":[121,163,182],"causes":[122],"morphological":[123],"increases":[126],"on":[128],"soft":[129],"agar,":[130],"independent":[131],"ERK":[133],"activity.":[134],"The":[135],"transformed":[136],"cell":[137],"lines":[138],"show":[139],"increased":[140],"matrix":[143],"metalloproteinases":[144],"2":[145],"9":[147],"cathepsin":[149],"L,":[150],"proteinases":[151],"have":[153],"been":[154],"implicated":[155],"process.":[159],"We":[160],"tested":[161],"NIH3T3":[162,210],"transfected":[164],"with":[165],"[Asp218]":[167],"Asp222]":[170],"for":[171],"after":[174],"i.v.":[175],"injection":[176],"into":[177],"athymic":[178],"mice.":[179],"Parental":[180],"formed":[183,194],"no":[184],"tumors":[185],"grossly":[186],"histologically.":[188],"However,":[189],"all":[190],"transformants":[193],"macroscopic":[195],"metastases.":[196],"Thus,":[197],"like":[198],"Ras,":[200],"tumorigenic":[205,222,231],"These":[212],"refine":[214],"mechanism":[216],"through":[217],"could":[220],"(ie.,":[226],"critical":[228],"determinants":[229],"downstream":[236],"MEK1).":[238]},"counts_by_year":[{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2018,"cited_by_count":4},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":3},{"year":2015,"cited_by_count":2},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":4}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}