{"id":"https://openalex.org/W2144647173","doi":"https://doi.org/10.1128/aem.61.11.3849-3855.1995","title":"Development of a PCR protocol for sensitive detection of Cryptosporidium oocysts in water samples","display_name":"Development of a PCR protocol for sensitive detection of Cryptosporidium oocysts in water samples","publication_year":1995,"publication_date":"1995-11-01","ids":{"openalex":"https://openalex.org/W2144647173","doi":"https://doi.org/10.1128/aem.61.11.3849-3855.1995","mag":"2144647173","pmid":"https://pubmed.ncbi.nlm.nih.gov/8526496","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/167689"},"language":"en","primary_location":{"id":"doi:10.1128/aem.61.11.3849-3855.1995","doi":"https://doi.org/10.1128/aem.61.11.3849-3855.1995","is_oa":true,"landing_page_url":"https://doi.org/10.1128/aem.61.11.3849-3855.1995","pdf_url":"https://aem.asm.org/content/aem/61/11/3849.full.pdf","source":{"id":"https://openalex.org/S158228368","display_name":"Applied and Environmental Microbiology","issn_l":"0099-2240","issn":["0099-2240","1070-6291","1098-5336"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied and Environmental Microbiology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://aem.asm.org/content/aem/61/11/3849.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"D W Johnson","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I75821886","display_name":"University of South Florida St. Petersburg","ror":"https://ror.org/016gp6x28","country_code":"US","type":"education","lineage":["https://openalex.org/I75821886"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"D W Johnson","raw_affiliation_strings":["Department of Marine Science, University of South Florida, St. Petersburg 33701-5016, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Marine Science, University of South Florida, St. Petersburg 33701-5016, USA","institution_ids":["https://openalex.org/I75821886"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5108338815","display_name":"Norman J. Pieni\u0105\u017cek","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I75821886","display_name":"University of South Florida St. Petersburg","ror":"https://ror.org/016gp6x28","country_code":"US","type":"education","lineage":["https://openalex.org/I75821886"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"N J Pieniazek","raw_affiliation_strings":["Department of Marine Science, University of South Florida, St. Petersburg 33701-5016, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Marine Science, University of South Florida, St. Petersburg 33701-5016, USA","institution_ids":["https://openalex.org/I75821886"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5031159945","display_name":"Dale Warren Griffin","orcid":"https://orcid.org/0000-0003-1719-5812","observed_orcids":["https://orcid.org/0000-0003-1719-5812"]},"institutions":[{"id":"https://openalex.org/I75821886","display_name":"University of South Florida St. Petersburg","ror":"https://ror.org/016gp6x28","country_code":"US","type":"education","lineage":["https://openalex.org/I75821886"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"D W Griffin","raw_affiliation_strings":["Department of Marine Science, University of South Florida, St. Petersburg 33701-5016, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Marine Science, University of South Florida, St. Petersburg 33701-5016, USA","institution_ids":["https://openalex.org/I75821886"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5068181301","display_name":"L Misener","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I75821886","display_name":"University of South Florida St. Petersburg","ror":"https://ror.org/016gp6x28","country_code":"US","type":"education","lineage":["https://openalex.org/I75821886"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"L Misener","raw_affiliation_strings":["Department of Marine Science, University of South Florida, St. Petersburg 33701-5016, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Marine Science, University of South Florida, St. Petersburg 33701-5016, USA","institution_ids":["https://openalex.org/I75821886"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5083906163","display_name":"Joan B. Rose","orcid":"https://orcid.org/0000-0002-5871-0211","observed_orcids":["https://orcid.org/0000-0002-5871-0211"]},"institutions":[{"id":"https://openalex.org/I75821886","display_name":"University of South Florida St. Petersburg","ror":"https://ror.org/016gp6x28","country_code":"US","type":"education","lineage":["https://openalex.org/I75821886"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"J B Rose","raw_affiliation_strings":["Department of Marine Science, University of South Florida, St. Petersburg 33701-5016, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Marine Science, University of South Florida, St. Petersburg 33701-5016, USA","institution_ids":["https://openalex.org/I75821886"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I75821886"],"apc_list":null,"apc_paid":null,"fwci":10.9488,"has_fulltext":false,"cited_by_count":350,"citation_normalized_percentile":{"value":0.98985696,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"61","issue":"11","first_page":"3849","last_page":"3855"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11086","display_name":"Parasitic Infections and Diagnostics","score":0.9897000193595886,"subfield":{"id":"https://openalex.org/subfields/2405","display_name":"Parasitology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11086","display_name":"Parasitic Infections and Diagnostics","score":0.9897000193595886,"subfield":{"id":"https://openalex.org/subfields/2405","display_name":"Parasitology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11331","display_name":"Fecal contamination and water quality","score":0.008200000040233135,"subfield":{"id":"https://openalex.org/subfields/2312","display_name":"Water Science and Technology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10976","display_name":"Viral gastroenteritis research and epidemiology","score":0.0013000000035390258,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cryptosporidium","display_name":"Cryptosporidium","score":0.9829999804496765},{"id":"https://openalex.org/keywords/oocyst-detection","display_name":"oocyst detection","score":0.8809999823570251},{"id":"https://openalex.org/keywords/pcr-assay","display_name":"PCR testing","score":0.7929999828338623},{"id":"https://openalex.org/keywords/18s-rrna","display_name":"18S rRNA","score":0.8659999966621399},{"id":"https://openalex.org/keywords/waterborne-pathogens","display_name":"waterborne pathogens","score":0.578000009059906},{"id":"https://openalex.org/keywords/water-quality","display_name":"water quality","score":0.4009999930858612},{"id":"https://openalex.org/keywords/flow-cytometry","display_name":"flow cytometry","score":0.5600000023841858},{"id":"https://openalex.org/keywords/immunofluorescence-comparison","display_name":"immunofluorescence comparison","score":0.8479999899864197}],"concepts":[{"id":"https://openalex.org/C2778877831","wikidata":"https://www.wikidata.org/wiki/Q133078","display_name":"Cryptosporidium","level":3,"score":0.8877924084663391},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7093613147735596},{"id":"https://openalex.org/C2777795018","wikidata":"https://www.wikidata.org/wiki/Q134734","display_name":"Cryptosporidium parvum","level":2,"score":0.6528472304344177},{"id":"https://openalex.org/C111824103","wikidata":"https://www.wikidata.org/wiki/Q7086828","display_name":"Oligomer restriction","level":4,"score":0.5477619767189026},{"id":"https://openalex.org/C2781023229","wikidata":"https://www.wikidata.org/wiki/Q193030","display_name":"Apicomplexa","level":4,"score":0.5240153074264526},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5102121829986572},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.49612078070640564},{"id":"https://openalex.org/C2778236600","wikidata":"https://www.wikidata.org/wiki/Q592324","display_name":"Immunofluorescence","level":3,"score":0.4912695288658142},{"id":"https://openalex.org/C129312508","wikidata":"https://www.wikidata.org/wiki/Q133686","display_name":"Oligonucleotide","level":3,"score":0.46128007769584656},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.44361597299575806},{"id":"https://openalex.org/C553184892","wikidata":"https://www.wikidata.org/wiki/Q1141429","display_name":"Flow cytometry","level":2,"score":0.4368390440940857},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.382485568523407},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.2524808645248413},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.16094526648521423},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.09395915269851685},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.09255850315093994},{"id":"https://openalex.org/C2778371730","wikidata":"https://www.wikidata.org/wiki/Q311383","display_name":"Plasmodium falciparum","level":3,"score":0.0709114670753479},{"id":"https://openalex.org/C61716771","wikidata":"https://www.wikidata.org/wiki/Q496","display_name":"Feces","level":2,"score":0.0},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.0},{"id":"https://openalex.org/C2778048844","wikidata":"https://www.wikidata.org/wiki/Q12156","display_name":"Malaria","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003458","descriptor_name":"Cryptosporidium","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D003458","descriptor_name":"Cryptosporidium","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D005069","descriptor_name":"Evaluation Studies as Topic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012337","descriptor_name":"RNA, Ribosomal, 18S","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012680","descriptor_name":"Sensitivity and Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014867","descriptor_name":"Water","qualifier_ui":"Q000469","qualifier_name":"parasitology","is_major_topic":true},{"descriptor_ui":"D014881","descriptor_name":"Water Supply","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015342","descriptor_name":"DNA Probes","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D016054","descriptor_name":"DNA, Protozoan","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":"Q000706","qualifier_name":"statistics & numerical data","is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1128/aem.61.11.3849-3855.1995","doi":"https://doi.org/10.1128/aem.61.11.3849-3855.1995","is_oa":true,"landing_page_url":"https://doi.org/10.1128/aem.61.11.3849-3855.1995","pdf_url":"https://aem.asm.org/content/aem/61/11/3849.full.pdf","source":{"id":"https://openalex.org/S158228368","display_name":"Applied and Environmental Microbiology","issn_l":"0099-2240","issn":["0099-2240","1070-6291","1098-5336"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied and Environmental Microbiology","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:392056","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC167689","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:167689","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/167689","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Appl Environ Microbiol","raw_type":"Text"},{"id":"pmid:8526496","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8526496","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied and environmental microbiology","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1128/aem.61.11.3849-3855.1995","doi":"https://doi.org/10.1128/aem.61.11.3849-3855.1995","is_oa":true,"landing_page_url":"https://doi.org/10.1128/aem.61.11.3849-3855.1995","pdf_url":"https://aem.asm.org/content/aem/61/11/3849.full.pdf","source":{"id":"https://openalex.org/S158228368","display_name":"Applied and Environmental Microbiology","issn_l":"0099-2240","issn":["0099-2240","1070-6291","1098-5336"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied and Environmental Microbiology","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/6","score":0.8571000099182129,"display_name":"Clean water and sanitation"},{"id":"https://metadata.un.org/sdg/3","score":0.428600013256073,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":false},"content_urls":{"pdf":"https://content.openalex.org/works/W2144647173.pdf"},"referenced_works_count":32,"referenced_works":["https://openalex.org/W196014493","https://openalex.org/W1017502435","https://openalex.org/W1480087692","https://openalex.org/W1481394337","https://openalex.org/W1483738833","https://openalex.org/W1514941246","https://openalex.org/W1558203474","https://openalex.org/W1562008489","https://openalex.org/W1563383052","https://openalex.org/W1577622626","https://openalex.org/W1597666972","https://openalex.org/W1614332721","https://openalex.org/W1879927595","https://openalex.org/W1994383906","https://openalex.org/W1996730714","https://openalex.org/W2019402469","https://openalex.org/W2022385481","https://openalex.org/W2036903205","https://openalex.org/W2038277732","https://openalex.org/W2049118482","https://openalex.org/W2060811718","https://openalex.org/W2062165684","https://openalex.org/W2118350683","https://openalex.org/W2148084350","https://openalex.org/W2156380818","https://openalex.org/W2157602381","https://openalex.org/W2284474987","https://openalex.org/W2323094357","https://openalex.org/W2411719388","https://openalex.org/W2916270096","https://openalex.org/W3024372856","https://openalex.org/W4235316562"],"related_works":["https://openalex.org/W2353264579","https://openalex.org/W1017502435","https://openalex.org/W2102586116","https://openalex.org/W1994919917","https://openalex.org/W1572787286","https://openalex.org/W2045711181","https://openalex.org/W2003484269","https://openalex.org/W2041141622","https://openalex.org/W2101343693","https://openalex.org/W2078048454"],"abstract_inverted_index":{"The":[0,40,65,88],"development":[1],"of":[2,6,11,24,32,50,55,90,126,141,144,173],"a":[3,22,35,61],"reliable":[4],"method":[5],"using":[7],"PCR":[8,41,92,128,162],"for":[9,171],"detection":[10,49,172],"Cryptosporidium":[12,51,145],"oocysts":[13,59,72,146,174],"in":[14,73,94,147,153,175],"environmental":[15,97],"samples":[16,99,149],"with":[17,133,158],"oligonucleotide":[18,135],"primers":[19],"which":[20],"amplify":[21],"portion":[23],"the":[25,28,91,127,142,151],"sequence":[26],"encoding":[27],"small":[29],"(18S)":[30],"subunit":[31],"rRNA":[33],"producing":[34],"435-bp":[36],"product":[37,129],"was":[38,43,100,108,118,156,163],"demonstrated.":[39],"assay":[42,66,93],"found":[44,164],"to":[45,70,102,122,165],"provide":[46],"highly":[47],"genus-specific":[48],"spp.":[52],"after":[53],"release":[54],"nucleic":[56],"acids":[57],"from":[58,150],"by":[60,79,84,110,124,138],"simple":[62],"freeze-thaw":[63],"procedure.":[64],"routinely":[67],"detected":[68,137],"1":[69],"10":[71],"purified":[74],"oocyst":[75],"preparations,":[76],"as":[77,167,169],"shown":[78],"direct":[80],"microscopic":[81],"counts":[82],"and":[83,161],"an":[85,134],"immunofluorescence":[86,170],"assay.":[87],"sensitivity":[89],"some":[95],"seeded":[96],"water":[98,148],"up":[101],"1,000-fold":[103,123],"lower.":[104],"However,":[105],"this":[106,159],"interference":[107],"eliminated":[109],"either":[111],"flow":[112],"cytometry":[113],"or":[114],"magnetic-antibody":[115],"capture.":[116],"Sensitivity":[117],"also":[119],"improved":[120],"10-":[121],"probing":[125],"on":[130],"dot":[131],"blots":[132],"probe":[136],"chemiluminescence.":[139],"Confirmation":[140],"presence":[143],"outbreak":[152],"Milwaukee,":[154],"Wis.,":[155],"obtained":[157],"technique,":[160],"be":[166],"sensitive":[168],"wastewater":[176],"concentrates.":[177]},"counts_by_year":[{"year":2026,"cited_by_count":4},{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":6},{"year":2023,"cited_by_count":4},{"year":2022,"cited_by_count":11},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":3},{"year":2018,"cited_by_count":6},{"year":2017,"cited_by_count":4},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":5},{"year":2014,"cited_by_count":6},{"year":2013,"cited_by_count":11},{"year":2012,"cited_by_count":13}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}