{"id":"https://openalex.org/W2141069488","doi":"https://doi.org/10.1590/s0100-41582004000300013","title":"Produ\u00e7\u00e3o de mic\u00e9lio de Crinipellis perniciosa em quatro meios de cultura, visando extra\u00e7\u00e3o de DNA","display_name":"Produ\u00e7\u00e3o de mic\u00e9lio de Crinipellis perniciosa em quatro meios de cultura, visando extra\u00e7\u00e3o de DNA","publication_year":2004,"publication_date":"2004-06-01","ids":{"openalex":"https://openalex.org/W2141069488","doi":"https://doi.org/10.1590/s0100-41582004000300013","mag":"2141069488"},"language":"pt","primary_location":{"id":"doi:10.1590/s0100-41582004000300013","doi":"https://doi.org/10.1590/s0100-41582004000300013","is_oa":true,"landing_page_url":"https://doi.org/10.1590/s0100-41582004000300013","pdf_url":"https://www.scielo.br/j/fb/a/znGMf6kytKvMYMTfMwmtL3g/?lang=pt&format=pdf","source":{"id":"https://openalex.org/S85248228","display_name":"Fitopatologia Brasileira","issn_l":"0100-4158","issn":["0100-4158","1678-4677"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","norway-1"],"host_organization":"https://openalex.org/P4310319965","host_organization_name":"Springer Nature","host_organization_lineage":["https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Nature"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Fitopatologia Brasileira","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"https://www.scielo.br/j/fb/a/znGMf6kytKvMYMTfMwmtL3g/?lang=pt&format=pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5079587299","display_name":"F\u00e1bio Gelape Faleiro","orcid":"https://orcid.org/0000-0001-8901-6428","observed_orcids":["https://orcid.org/0000-0001-8901-6428"]},"institutions":[{"id":"https://openalex.org/I4405283400","display_name":"Embrapa Cerrados","ror":"https://ror.org/01km6g468","country_code":"BR","type":"government","lineage":["https://openalex.org/I199691007","https://openalex.org/I4405283400"]}],"countries":["BR"],"is_corresponding":true,"raw_author_name":"F\u00e1bio G. Faleiro","raw_affiliation_strings":["Laborat\u00f3rio de Biotecnologia; Embrapa Cerrados"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laborat\u00f3rio de Biotecnologia; Embrapa Cerrados","institution_ids":["https://openalex.org/I4405283400"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5021187524","display_name":"Givaldo Rocha Niella","orcid":"https://orcid.org/0000-0002-6659-7060","observed_orcids":["https://orcid.org/0000-0002-6659-7060"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Givaldo R. Niella","raw_affiliation_strings":["Centro de Pesquisas do Cacau","Centro de Pesquisas do Cacau, Itabuna"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Centro de Pesquisas do Cacau","institution_ids":[]},{"raw_affiliation_string":"Centro de Pesquisas do Cacau, Itabuna","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5024473457","display_name":"Ana Rosa R.N. Cerqueira","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Ana Rosa R.N. Cerqueira","raw_affiliation_strings":["Centro de Pesquisas do Cacau","Centro de Pesquisas do Cacau, Itabuna"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Centro de Pesquisas do Cacau","institution_ids":[]},{"raw_affiliation_string":"Centro de Pesquisas do Cacau, Itabuna","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5000031231","display_name":"Virg\u00ednia Oliveira Damaceno","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Virg\u00ednia O. Damaceno","raw_affiliation_strings":["Centro de Pesquisas do Cacau","Centro de Pesquisas do Cacau, Itabuna"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Centro de Pesquisas do Cacau","institution_ids":[]},{"raw_affiliation_string":"Centro de Pesquisas do Cacau, Itabuna","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5108523666","display_name":"L.M.C. Gomes","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Luana M.C. Gomes","raw_affiliation_strings":["Laborat\u00f3rio de Biotecnologia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laborat\u00f3rio de Biotecnologia","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5059394250","display_name":"A.S.G. Faleiro","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Alessandra S.G. Faleiro","raw_affiliation_strings":["Laborat\u00f3rio de Biotecnologia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laborat\u00f3rio de Biotecnologia","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5079587299"],"corresponding_institution_ids":["https://openalex.org/I4405283400"],"apc_list":null,"apc_paid":null,"fwci":0.0974,"has_fulltext":true,"cited_by_count":3,"citation_normalized_percentile":{"value":0.35453193,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"29","issue":"3","first_page":"312","last_page":"315"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.27559998631477356,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.27559998631477356,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10825","display_name":"Plant Pathogens and Fungal Diseases","score":0.23600000143051147,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11085","display_name":"Fungal Biology and Applications","score":0.20399999618530273,"subfield":{"id":"https://openalex.org/subfields/2736","display_name":"Pharmacology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/crinipellis-perniciosa","display_name":"Crinipellis perniciosa","score":0.9990000128746033},{"id":"https://openalex.org/keywords/culture-medium","display_name":"culture media","score":0.7730000019073486},{"id":"https://openalex.org/keywords/dna-extraction","display_name":"DNA extraction","score":0.9330000281333923},{"id":"https://openalex.org/keywords/dna-quality","display_name":"DNA quality","score":0.5370000004768372},{"id":"https://openalex.org/keywords/dna-integrity","display_name":"DNA integrity","score":0.800000011920929},{"id":"https://openalex.org/keywords/phenol-extraction","display_name":"phenol extraction","score":0.7170000076293945},{"id":"https://openalex.org/keywords/yeast-extract","display_name":"yeast extract","score":0.5979999899864197},{"id":"https://openalex.org/keywords/malt-extract","display_name":"malt extract","score":0.9549999833106995},{"id":"https://openalex.org/keywords/potato-dextrose-broth","display_name":"potato dextrose broth","score":0.9100000262260437},{"id":"https://openalex.org/keywords/dna-amplification","display_name":"DNA amplification","score":0.3199999928474426},{"id":"https://openalex.org/keywords/fungal-dna","display_name":"fungal DNA","score":0.3100000023841858}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.44248923659324646},{"id":"https://openalex.org/C144027150","wikidata":"https://www.wikidata.org/wiki/Q48803","display_name":"Horticulture","level":1,"score":0.4215199649333954},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.40279650688171387},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.38821911811828613}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.1590/s0100-41582004000300013","doi":"https://doi.org/10.1590/s0100-41582004000300013","is_oa":true,"landing_page_url":"https://doi.org/10.1590/s0100-41582004000300013","pdf_url":"https://www.scielo.br/j/fb/a/znGMf6kytKvMYMTfMwmtL3g/?lang=pt&format=pdf","source":{"id":"https://openalex.org/S85248228","display_name":"Fitopatologia Brasileira","issn_l":"0100-4158","issn":["0100-4158","1678-4677"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","norway-1"],"host_organization":"https://openalex.org/P4310319965","host_organization_name":"Springer Nature","host_organization_lineage":["https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Nature"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Fitopatologia Brasileira","raw_type":"journal-article"},{"id":"pmh:oai:scielo:S0100-41582004000300013","doi":null,"is_oa":true,"landing_page_url":"http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582004000300013","pdf_url":null,"source":{"id":"https://openalex.org/S4306402308","display_name":"The Scientific Electronic Library Online (Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210116822","host_organization_name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","host_organization_lineage":["https://openalex.org/I4210116822"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Fitopatologia Brasileira v.29 n.3 2004","raw_type":"info:eu-repo/semantics/other"}],"best_oa_location":{"id":"doi:10.1590/s0100-41582004000300013","doi":"https://doi.org/10.1590/s0100-41582004000300013","is_oa":true,"landing_page_url":"https://doi.org/10.1590/s0100-41582004000300013","pdf_url":"https://www.scielo.br/j/fb/a/znGMf6kytKvMYMTfMwmtL3g/?lang=pt&format=pdf","source":{"id":"https://openalex.org/S85248228","display_name":"Fitopatologia Brasileira","issn_l":"0100-4158","issn":["0100-4158","1678-4677"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","norway-1"],"host_organization":"https://openalex.org/P4310319965","host_organization_name":"Springer Nature","host_organization_lineage":["https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Nature"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Fitopatologia Brasileira","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.9208999872207642,"id":"https://metadata.un.org/sdg/2","display_name":"Zero hunger"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2141069488.pdf","grobid_xml":"https://content.openalex.org/works/W2141069488.grobid-xml"},"referenced_works_count":8,"referenced_works":["https://openalex.org/W1480753239","https://openalex.org/W1963953102","https://openalex.org/W1996281280","https://openalex.org/W2137003031","https://openalex.org/W2144634347","https://openalex.org/W2171114940","https://openalex.org/W2314225431","https://openalex.org/W2540206787"],"related_works":["https://openalex.org/W2245066657","https://openalex.org/W1906285416","https://openalex.org/W4283800896","https://openalex.org/W2357451306","https://openalex.org/W2755400686","https://openalex.org/W2195750155","https://openalex.org/W2488594250","https://openalex.org/W2342108202","https://openalex.org/W7211961538","https://openalex.org/W2891924523"],"abstract_inverted_index":{"A":[0,157,175,218],"produ\u00e7\u00e3o":[1],"de":[2,12,14,33,39,46,52,59,63,68,81,86,96,99,104,106,108,122,170,177,182,201,214,225,231,243],"massa":[3,40],"micelial":[4,41,91],"\u00e9":[5],"o":[6,50,112,130,141,145,250],"primeiro":[7],"passo":[8],"para":[9,19],"obter":[10],"amostras":[11],"DNA":[13,32,142,160,197,226,230],"qualidade":[15,30,233],"e":[16,28,75,78,115,120,140,165,185,194,207,234],"quantidade":[17,27,176,236],"suficiente":[18,237],"an\u00e1lises":[20],"moleculares.":[21],"Neste":[22],"trabalho,":[23],"objetivou-se":[24],"analisar":[25],"a":[26,29,37,94,117,150,166,199,223,241,253],"do":[31,147,159,189],"Crinipellis":[34],"perniciosa":[35],"extra\u00eddo":[36,143,198,240],"partir":[38,95,200,242],"obtida":[42],"em":[43,168,235,246],"quatro":[44],"meios":[45,58,181,192,205,247],"cultura.":[47],"Foi":[48,128],"avaliado":[49],"peso":[51],"mic\u00e9lio":[53,100,136,178,202,244],"liofilizado":[54,139],"produzido":[55,137,203,245],"nos":[56,180,191,204],"seguintes":[57],"cultura:":[60],"1.":[61],"extrato":[62,67,80],"levedura":[64],"(2,5%);":[65,70],"2.":[66],"malte":[69,82],"3.":[71,195],"BD":[72,84],"(batata":[73],"20%":[74],"dextrose":[76],"2%)":[77],"4.":[79],"+":[83],"(50%":[85],"cada":[87],"meio).":[88],"O":[89,135,196],"crescimento":[90],"foi":[92,138,187],"iniciado":[93],"um":[97],"disco":[98],"colocado":[101],"no":[102],"centro":[103],"placas":[105],"Petri":[107],"90":[109],"mm":[110],"contendo":[111],"meio":[113],"testado":[114],"mantidas":[116],"25":[118],"\u00baC":[119],"fotoper\u00edodo":[121],"12":[123],"h,":[124],"por":[125],"dez":[126,133],"dias.":[127],"utilizado":[129],"DIC":[131],"com":[132,149,153,220,257],"repeti\u00e7\u00f5es.":[134],"utilizando-se":[144],"m\u00e9todo":[146],"SDS":[148],"desproteiniza\u00e7\u00e3o":[151,219,256],"feita":[152],"ou":[154],"sem":[155,252],"fenol.":[156,258],"pureza":[158],"baseada":[161],"na":[162],"rela\u00e7\u00e3o":[163],"A260/A280":[164],"integridade":[167],"gel":[169],"agarose":[171],"0,8%":[172],"foram":[173],"analisadas.":[174],"produzida":[179],"cultura":[183],"1":[184],"4":[186],"maior":[188,210],"que":[190],"2":[193,206],"3":[208],"apresentou":[209],"integridade,":[211],"obtendo-se":[212],"produtos":[213],"amplifica\u00e7\u00e3o":[215],"mais":[216,227],"n\u00edtidos.":[217],"fenol":[221],"possibilitou":[222],"extra\u00e7\u00e3o":[224],"puro.":[228],"Por\u00e9m,":[229],"\u00f3tima":[232],"pode":[238],"ser":[239],"baratos":[248],"como":[249],"BD,":[251],"necessidade":[254],"da":[255]},"counts_by_year":[{"year":2015,"cited_by_count":1}],"updated_date":"2026-11-12T12:28:57.059296","created_date":"2016-06-24T00:00:00"}