{"id":"https://openalex.org/W2139868575","doi":null,"title":"Screening and characterisation of wine related enzymes produced by wine associated lactic acid bacteria","display_name":"Screening and characterisation of wine related enzymes produced by wine associated lactic acid bacteria","publication_year":2007,"publication_date":"2007-03-01","ids":{"openalex":"https://openalex.org/W2139868575","mag":"2139868575"},"language":"en","primary_location":{"id":"pmh:oai:scholar.sun.ac.za:10019.1/2363","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/10019.1/2363","pdf_url":null,"source":{"id":"https://openalex.org/S4306400300","display_name":"SUNScholar (Stellenbosch University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I26092322","host_organization_name":"Stellenbosch University","host_organization_lineage":["https://openalex.org/I26092322"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5028423164","display_name":"Phillip Senzo Mtshali","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Phillip Senzo Mtshali","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5028423164"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":9,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10750","display_name":"Fermentation and Sensory Analysis","score":0.9740999937057495,"subfield":{"id":"https://openalex.org/subfields/1106","display_name":"Food Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10750","display_name":"Fermentation and Sensory Analysis","score":0.9740999937057495,"subfield":{"id":"https://openalex.org/subfields/1106","display_name":"Food Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10141","display_name":"Probiotics and Fermented Foods","score":0.01119999960064888,"subfield":{"id":"https://openalex.org/subfields/1106","display_name":"Food Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.008999999612569809,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/malolactic-fermentation","display_name":"malolactic fermentation","score":0.9129999876022339},{"id":"https://openalex.org/keywords/lactic-acid-bacteria","display_name":"lactic acid bacteria","score":0.9419999718666077},{"id":"https://openalex.org/keywords/wine","display_name":"wine","score":0.6169999837875366},{"id":"https://openalex.org/keywords/beta-glucosidase","display_name":"\u03b2-glucosidase","score":0.9430000185966492},{"id":"https://openalex.org/keywords/glucanase","display_name":"glucanase","score":0.9480000138282776},{"id":"https://openalex.org/keywords/serine-protease","display_name":"serine protease","score":0.9259999990463257},{"id":"https://openalex.org/keywords/esterase","display_name":"esterase","score":0.9980000257492065},{"id":"https://openalex.org/keywords/lipase","display_name":"lipase","score":0.9399999976158142},{"id":"https://openalex.org/keywords/enzyme-screening","display_name":"enzyme screening","score":0.5239999890327454},{"id":"https://openalex.org/keywords/lactiplantibacillus-plantarum","display_name":"Lactobacillus plantarum","score":0.7979999780654907},{"id":"https://openalex.org/keywords/south-african-wine","display_name":"South African wine","score":0.718999981880188}],"concepts":[{"id":"https://openalex.org/C78945660","wikidata":"https://www.wikidata.org/wiki/Q654005","display_name":"Malolactic fermentation","level":4,"score":0.9332320690155029},{"id":"https://openalex.org/C55952523","wikidata":"https://www.wikidata.org/wiki/Q3014419","display_name":"Wine","level":2,"score":0.6618289947509766},{"id":"https://openalex.org/C2775920511","wikidata":"https://www.wikidata.org/wiki/Q161249","display_name":"Lactic acid","level":3,"score":0.5789759755134583},{"id":"https://openalex.org/C100544194","wikidata":"https://www.wikidata.org/wiki/Q41760","display_name":"Fermentation","level":2,"score":0.5444106459617615},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5368353128433228},{"id":"https://openalex.org/C2777261171","wikidata":"https://www.wikidata.org/wiki/Q418750","display_name":"Esterase","level":3,"score":0.49024420976638794},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.47322240471839905},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.47214630246162415},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.46796590089797974},{"id":"https://openalex.org/C2776714187","wikidata":"https://www.wikidata.org/wiki/Q212410","display_name":"Protease","level":3,"score":0.4454174041748047},{"id":"https://openalex.org/C2777806559","wikidata":"https://www.wikidata.org/wiki/Q559395","display_name":"Lactobacillus brevis","level":5,"score":0.43777233362197876},{"id":"https://openalex.org/C2778985185","wikidata":"https://www.wikidata.org/wiki/Q1756682","display_name":"Lactobacillus plantarum","level":4,"score":0.42493975162506104},{"id":"https://openalex.org/C59920403","wikidata":"https://www.wikidata.org/wiki/Q698044","display_name":"Aroma of wine","level":3,"score":0.4140552282333374},{"id":"https://openalex.org/C2780206646","wikidata":"https://www.wikidata.org/wiki/Q1061596","display_name":"Lactobacillus","level":3,"score":0.4133261442184448},{"id":"https://openalex.org/C31903555","wikidata":"https://www.wikidata.org/wiki/Q1637030","display_name":"Food science","level":1,"score":0.40656283497810364},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.35799944400787354},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.33690115809440613},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.10004612803459167}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:scholar.sun.ac.za:10019.1/2363","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/10019.1/2363","pdf_url":null,"source":{"id":"https://openalex.org/S4306400300","display_name":"SUNScholar (Stellenbosch University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I26092322","host_organization_name":"Stellenbosch University","host_organization_lineage":["https://openalex.org/I26092322"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},{"id":"mag:2139868575","doi":null,"is_oa":false,"landing_page_url":"https://scholar.sun.ac.za/bitstream/10019.1/2363/1/mtshali_screening_2007.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":131,"referenced_works":["https://openalex.org/W75130528","https://openalex.org/W94004571","https://openalex.org/W138223345","https://openalex.org/W204912039","https://openalex.org/W213901614","https://openalex.org/W563718433","https://openalex.org/W1448366202","https://openalex.org/W1497475797","https://openalex.org/W1517133193","https://openalex.org/W1517391409","https://openalex.org/W1545519031","https://openalex.org/W1556333526","https://openalex.org/W1568037241","https://openalex.org/W1591627894","https://openalex.org/W1593744665","https://openalex.org/W1618639643","https://openalex.org/W1717421490","https://openalex.org/W1740666950","https://openalex.org/W1753564585","https://openalex.org/W1762307288","https://openalex.org/W1772341111","https://openalex.org/W1793250009","https://openalex.org/W1831628839","https://openalex.org/W1840689075","https://openalex.org/W1867122191","https://openalex.org/W1890527771","https://openalex.org/W1917187031","https://openalex.org/W1962356367","https://openalex.org/W1963677423","https://openalex.org/W1966205402","https://openalex.org/W1972222322","https://openalex.org/W1975100795","https://openalex.org/W1976033466","https://openalex.org/W1978036678","https://openalex.org/W1980197882","https://openalex.org/W1983539649","https://openalex.org/W1985167768","https://openalex.org/W1990335722","https://openalex.org/W1992103842","https://openalex.org/W1995092770","https://openalex.org/W1996922186","https://openalex.org/W1997354422","https://openalex.org/W1998165249","https://openalex.org/W1999349622","https://openalex.org/W2002056977","https://openalex.org/W2012235484","https://openalex.org/W2013268794","https://openalex.org/W2013652634","https://openalex.org/W2018706034","https://openalex.org/W2020862954","https://openalex.org/W2021333226","https://openalex.org/W2021831024","https://openalex.org/W2023270760","https://openalex.org/W2024892045","https://openalex.org/W2024892358","https://openalex.org/W2028775549","https://openalex.org/W2032036837","https://openalex.org/W2036882962","https://openalex.org/W2045131760","https://openalex.org/W2045157173","https://openalex.org/W2049867177","https://openalex.org/W2054290110","https://openalex.org/W2059640818","https://openalex.org/W2059718350","https://openalex.org/W2060146910","https://openalex.org/W2062304440","https://openalex.org/W2063527024","https://openalex.org/W2068544447","https://openalex.org/W2071331513","https://openalex.org/W2071395087","https://openalex.org/W2072622688","https://openalex.org/W2073115823","https://openalex.org/W2078162188","https://openalex.org/W2079029116","https://openalex.org/W2079666385","https://openalex.org/W2081368930","https://openalex.org/W2092386858","https://openalex.org/W2098304985","https://openalex.org/W2098757779","https://openalex.org/W2098969939","https://openalex.org/W2099313531","https://openalex.org/W2099554570","https://openalex.org/W2102042758","https://openalex.org/W2105015569","https://openalex.org/W2110329577","https://openalex.org/W2114038147","https://openalex.org/W2114414482","https://openalex.org/W2116876124","https://openalex.org/W2123030953","https://openalex.org/W2131745182","https://openalex.org/W2133534556","https://openalex.org/W2135734084","https://openalex.org/W2146816220","https://openalex.org/W2147326175","https://openalex.org/W2149342156","https://openalex.org/W2151138139","https://openalex.org/W2152701541","https://openalex.org/W2152827306","https://openalex.org/W2163081580","https://openalex.org/W2165178233","https://openalex.org/W2166107869","https://openalex.org/W2167504496","https://openalex.org/W2182779358","https://openalex.org/W2277997277","https://openalex.org/W2283364323","https://openalex.org/W2287460235","https://openalex.org/W2288318076","https://openalex.org/W2340148085","https://openalex.org/W2343377825","https://openalex.org/W2346006950","https://openalex.org/W2412161145","https://openalex.org/W2414115325","https://openalex.org/W2432823362","https://openalex.org/W2480545164","https://openalex.org/W2487506503","https://openalex.org/W2493937535","https://openalex.org/W2497565083","https://openalex.org/W2516195304","https://openalex.org/W2517926008","https://openalex.org/W2519776350","https://openalex.org/W2530402980","https://openalex.org/W2531200395","https://openalex.org/W2565977214","https://openalex.org/W2602810669","https://openalex.org/W2629859407","https://openalex.org/W2764377347","https://openalex.org/W2764909720","https://openalex.org/W2773087427","https://openalex.org/W2948220143","https://openalex.org/W4304687046","https://openalex.org/W4316656804"],"related_works":["https://openalex.org/W1979842343","https://openalex.org/W2097197880","https://openalex.org/W4205724316","https://openalex.org/W2772235992","https://openalex.org/W3130788874","https://openalex.org/W7067515486","https://openalex.org/W4291449154","https://openalex.org/W1959879034","https://openalex.org/W4206708530","https://openalex.org/W2134367170"],"abstract_inverted_index":{"Malolactic":[0],"fermentation":[1,6,25],"(MLF)":[2],"is":[3,9,45],"the":[4,28,62,207,228,251,255,306],"secondary":[5],"process":[7,26],"which":[8,240],"normally":[10],"conducted":[11],"by":[12],"lactic":[13],"acid":[14,33,38],"bacteria":[15],"(LAB)":[16],"present":[17],"in":[18,70,89,174,313],"wine":[19,48,58,302],"after":[20],"alcoholic":[21],"fermentation.":[22],"The":[23,97,160],"former":[24],"involves":[27],"conversion":[29],"of":[30,68,87,109,254,274,309],"a":[31,36],"dicarboxylic":[32],"(malate)":[34],"to":[35,47,50,53,189,249],"monocarboxylic":[37],"(lactate)":[39],"and":[40,57,94,118,126,146,200,222,245,271,285],"carbon":[41],"dioxide":[42],"(CO2).":[43],"MLF":[44,81],"beneficial":[46],"due":[49],"its":[51],"contribution":[52],"deacidification,":[54],"microbial":[55],"stabilisation":[56],"aroma":[59],"formation,":[60],"with":[61,128,144,151,186,261,291],"latter":[63],"being":[64],"an":[65],"important":[66],"part":[67],"research":[69],"this":[71],"study.":[72],"LAB":[73,105,303],"isolated":[74],"from":[75,100,169,177,239,295],"South":[76],"African":[77],"wines":[78],"during":[79],"spontaneous":[80],"were":[82,121,136,154,167,211,237,264,280],"screened":[83,122,156],"for":[84,123,138,141,148,164,213,216,220,224,311],"different":[85,107],"enzymes":[86,110,310],"interest":[88],"winemaking":[90],"using":[91,157],"both":[92],"classical":[93],"molecular":[95,158],"techniques.":[96,159],"results":[98,299],"obtained":[99,132],"screening":[101],"have":[102],"shown":[103],"that":[104,134,230,277,301],"possess":[106],"combinations":[108],"such":[111],"as":[112,247],"\u03b2-":[113,124],"glucosidase,":[114],"protease,":[115,218],"esterase,":[116],"glucanase":[117,127,142,149],"lipase.":[119],"Isolates":[120,153],"glucosidase":[125],"plate":[129],"assays.":[130],"Results":[131],"showed":[133],"40%":[135,210],"positive":[137,212],"\u03b2-glucosidase,":[139,214],"80%":[140],"tested":[143,150],"carboxymethylcellulose,":[145],"65%":[147],"lichenan.":[152],"also":[155],"enzyme-specific":[161,262],"primers":[162,183,263],"used":[163,246],"PCR":[165],"amplifications":[166],"deduced":[168],"nucleotide":[170,292],"sequences":[171,275,279,287,293],"previously":[172],"identified":[173],"putative":[175],"genes":[176,185],"Lactobacillus":[178],"plantarum":[179],"WCFS1":[180],"strain.":[181],"These":[182,298],"amplified":[184],"sizes":[187],"corresponding":[188],"1392":[190],"bp":[191,195,198,202],"(\u03b2-":[192],"glucosidase),":[193],"1263":[194],"(protease),":[196],"1020":[197],"(esterase)":[199],"1644":[201],"(malolactic":[203],"enzyme).":[204],"Of":[205],"all":[206,232],"isolates":[208,229],"tested,":[209],"35%":[215],"serine":[217],"42%":[219],"esterase":[221],"36%":[223],"malolactic":[225],"enzyme.":[226],"Among":[227],"possessed":[231],"four":[233],"enzyme":[234],"genes,":[235],"11":[236],"selected":[238],"genomic":[241],"DNA":[242],"was":[243],"extracted":[244],"template":[248],"amplify":[250],"coding":[252],"regions":[253],"respective":[256],"genes.":[257],"Purified":[258],"amplicons":[259],"generated":[260],"further":[265],"cloned":[266],"into":[267],"pGEM-T":[268],"easy":[269],"vector":[270],"sequenced.":[272],"Analysis":[273],"revealed":[276],"gene":[278],"highly":[281],"conserved":[282],"between":[283],"species,":[284],"these":[286],"exhibited":[288],"significant":[289],"homology":[290],"available":[294],"GenBank":[296],"database.":[297],"suggest":[300],"can":[304],"be":[305],"potential":[307],"source":[308],"use":[312],"vinification.":[314]},"counts_by_year":[{"year":2020,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":2}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}