{"id":"https://openalex.org/W2139824980","doi":null,"title":"Chemical Synthesis of Affibody Molecules for Protein Detection and Molecular Imaging","display_name":"Chemical Synthesis of Affibody Molecules for Protein Detection and Molecular Imaging","publication_year":2008,"publication_date":"2008-01-01","ids":{"openalex":"https://openalex.org/W2139824980","mag":"2139824980"},"language":"en","primary_location":{"id":"pmh:oai:DiVA.org:kth-9626","doi":null,"is_oa":true,"landing_page_url":"http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-9626","pdf_url":null,"source":{"id":"https://openalex.org/S4306401559","display_name":"KTH Publication Database DiVA (KTH Royal Institute of Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I86987016","host_organization_name":"KTH Royal Institute of Technology","host_organization_lineage":["https://openalex.org/I86987016"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"info:eu-repo/semantics/doctoralThesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-9626","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5036215286","display_name":"Torun Ekblad","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"T. Ekblad","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5036215286"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.5971999764442444,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.5971999764442444,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10911","display_name":"Chemical Synthesis and Analysis","score":0.3921000063419342,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11395","display_name":"Radiopharmaceutical Chemistry and Applications","score":0.00279999990016222,"subfield":{"id":"https://openalex.org/subfields/2741","display_name":"Radiology, Nuclear Medicine and Imaging"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/affibody-molecules","display_name":"Affibody molecules","score":0.968999981880188},{"id":"https://openalex.org/keywords/protein-detection","display_name":"protein detection","score":0.7429999709129333},{"id":"https://openalex.org/keywords/molecular-imaging","display_name":"molecular imaging","score":0.7960000038146973},{"id":"https://openalex.org/keywords/z-domain","display_name":"Z-domain","score":0.5920000076293945},{"id":"https://openalex.org/keywords/solid-phase-peptide-synthesis","display_name":"solid-phase peptide synthesis","score":0.8980000019073486},{"id":"https://openalex.org/keywords/her2","display_name":"HER2","score":0.9480000138282776},{"id":"https://openalex.org/keywords/99mtc-radiolabeling","display_name":"99mTc radiolabeling","score":0.8560000061988831},{"id":"https://openalex.org/keywords/tissue-distribution","display_name":"tissue distribution","score":0.7300000190734863},{"id":"https://openalex.org/keywords/chemoselective-ligation","display_name":"chemoselective ligation","score":0.8190000057220459}],"concepts":[{"id":"https://openalex.org/C136339569","wikidata":"https://www.wikidata.org/wiki/Q1142297","display_name":"Molecular imaging","level":3,"score":0.5613909363746643},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3967408537864685},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.38901805877685547},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.37599819898605347},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.34934067726135254},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.18598735332489014},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.15853849053382874},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.08705386519432068},{"id":"https://openalex.org/C207001950","wikidata":"https://www.wikidata.org/wiki/Q141124","display_name":"In vivo","level":2,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:DiVA.org:kth-9626","doi":null,"is_oa":true,"landing_page_url":"http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-9626","pdf_url":null,"source":{"id":"https://openalex.org/S4306401559","display_name":"KTH Publication Database DiVA (KTH Royal Institute of Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I86987016","host_organization_name":"KTH Royal Institute of Technology","host_organization_lineage":["https://openalex.org/I86987016"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"info:eu-repo/semantics/doctoralThesis"},{"id":"mag:2139824980","doi":null,"is_oa":false,"landing_page_url":"http://kth.diva-portal.org/smash/record.jsf?pid=diva2:126762","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:DiVA.org:kth-9626","doi":null,"is_oa":true,"landing_page_url":"http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-9626","pdf_url":null,"source":{"id":"https://openalex.org/S4306401559","display_name":"KTH Publication Database DiVA (KTH Royal Institute of Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I86987016","host_organization_name":"KTH Royal Institute of Technology","host_organization_lineage":["https://openalex.org/I86987016"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"info:eu-repo/semantics/doctoralThesis"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":225,"referenced_works":["https://openalex.org/W9050420","https://openalex.org/W93334804","https://openalex.org/W97096330","https://openalex.org/W183966571","https://openalex.org/W1278793486","https://openalex.org/W1482029172","https://openalex.org/W1505550212","https://openalex.org/W1512671026","https://openalex.org/W1514973690","https://openalex.org/W1560265309","https://openalex.org/W1564113586","https://openalex.org/W1567848155","https://openalex.org/W1605490057","https://openalex.org/W1786523947","https://openalex.org/W1869516088","https://openalex.org/W1946074662","https://openalex.org/W1952620523","https://openalex.org/W1963903021","https://openalex.org/W1964019903","https://openalex.org/W1964321121","https://openalex.org/W1964538987","https://openalex.org/W1966326669","https://openalex.org/W1968998114","https://openalex.org/W1969554101","https://openalex.org/W1970386053","https://openalex.org/W1970786331","https://openalex.org/W1972018125","https://openalex.org/W1974217823","https://openalex.org/W1974464942","https://openalex.org/W1975565312","https://openalex.org/W1975674173","https://openalex.org/W1976067973","https://openalex.org/W1979507898","https://openalex.org/W1980299512","https://openalex.org/W1981947239","https://openalex.org/W1982834745","https://openalex.org/W1983257260","https://openalex.org/W1983494459","https://openalex.org/W1984086841","https://openalex.org/W1987119060","https://openalex.org/W1989586442","https://openalex.org/W1989672802","https://openalex.org/W1990264812","https://openalex.org/W1991233297","https://openalex.org/W1991673513","https://openalex.org/W1991711350","https://openalex.org/W1992271736","https://openalex.org/W1992931410","https://openalex.org/W1993870692","https://openalex.org/W1995190480","https://openalex.org/W1996427305","https://openalex.org/W1996820981","https://openalex.org/W1997674330","https://openalex.org/W1998003752","https://openalex.org/W1998251060","https://openalex.org/W1998986765","https://openalex.org/W2000905858","https://openalex.org/W2001324434","https://openalex.org/W2002168414","https://openalex.org/W2004065164","https://openalex.org/W2007637763","https://openalex.org/W2009179035","https://openalex.org/W2010649076","https://openalex.org/W2011261569","https://openalex.org/W2012536704","https://openalex.org/W2013360127","https://openalex.org/W2014764172","https://openalex.org/W2016375069","https://openalex.org/W2016519184","https://openalex.org/W2017260345","https://openalex.org/W2017649551","https://openalex.org/W2017916715","https://openalex.org/W2018107765","https://openalex.org/W2019656283","https://openalex.org/W2020451776","https://openalex.org/W2020816553","https://openalex.org/W2020830322","https://openalex.org/W2022558054","https://openalex.org/W2023167553","https://openalex.org/W2024298562","https://openalex.org/W2025551193","https://openalex.org/W2026089250","https://openalex.org/W2026234561","https://openalex.org/W2026597691","https://openalex.org/W2027134391","https://openalex.org/W2027269306","https://openalex.org/W2027617390","https://openalex.org/W2027700390","https://openalex.org/W2028320405","https://openalex.org/W2028994036","https://openalex.org/W2029790311","https://openalex.org/W2030522563","https://openalex.org/W2031022390","https://openalex.org/W2031154947","https://openalex.org/W2031606304","https://openalex.org/W2031988539","https://openalex.org/W2032579724","https://openalex.org/W2032875345","https://openalex.org/W2033692581","https://openalex.org/W2033887763","https://openalex.org/W2034078791","https://openalex.org/W2035758624","https://openalex.org/W2037275316","https://openalex.org/W2041513939","https://openalex.org/W2042400776","https://openalex.org/W2043370172","https://openalex.org/W2043734006","https://openalex.org/W2044434640","https://openalex.org/W2044532561","https://openalex.org/W2044682488","https://openalex.org/W2044765617","https://openalex.org/W2045562868","https://openalex.org/W2045777307","https://openalex.org/W2045994733","https://openalex.org/W2048333869","https://openalex.org/W2050003729","https://openalex.org/W2050519085","https://openalex.org/W2050574264","https://openalex.org/W2050717506","https://openalex.org/W2051471006","https://openalex.org/W2051845341","https://openalex.org/W2052652528","https://openalex.org/W2054052876","https://openalex.org/W2054258333","https://openalex.org/W2054592480","https://openalex.org/W2057520802","https://openalex.org/W2059845559","https://openalex.org/W2059939467","https://openalex.org/W2060542507","https://openalex.org/W2060982877","https://openalex.org/W2063484869","https://openalex.org/W2063823414","https://openalex.org/W2064434166","https://openalex.org/W2064851937","https://openalex.org/W2065620086","https://openalex.org/W2065625685","https://openalex.org/W2067013502","https://openalex.org/W2070360032","https://openalex.org/W2071979631","https://openalex.org/W2072268804","https://openalex.org/W2074341946","https://openalex.org/W2075097399","https://openalex.org/W2077031658","https://openalex.org/W2078487525","https://openalex.org/W2078896733","https://openalex.org/W2081306620","https://openalex.org/W2082377197","https://openalex.org/W2082717401","https://openalex.org/W2084429764","https://openalex.org/W2084728430","https://openalex.org/W2084855813","https://openalex.org/W2084938664","https://openalex.org/W2085943975","https://openalex.org/W2086506492","https://openalex.org/W2086863271","https://openalex.org/W2086889737","https://openalex.org/W2088435544","https://openalex.org/W2089595455","https://openalex.org/W2089625500","https://openalex.org/W2090383712","https://openalex.org/W2092942893","https://openalex.org/W2094517427","https://openalex.org/W2095553811","https://openalex.org/W2096176556","https://openalex.org/W2098689260","https://openalex.org/W2101298197","https://openalex.org/W2102369350","https://openalex.org/W2102893844","https://openalex.org/W2103719918","https://openalex.org/W2105199969","https://openalex.org/W2105315565","https://openalex.org/W2106787323","https://openalex.org/W2106876831","https://openalex.org/W2108796583","https://openalex.org/W2111196482","https://openalex.org/W2112035663","https://openalex.org/W2112295833","https://openalex.org/W2115754415","https://openalex.org/W2116520684","https://openalex.org/W2117037675","https://openalex.org/W2118502950","https://openalex.org/W2121358045","https://openalex.org/W2124503497","https://openalex.org/W2127068865","https://openalex.org/W2128130662","https://openalex.org/W2134178604","https://openalex.org/W2134450909","https://openalex.org/W2134647155","https://openalex.org/W2134758761","https://openalex.org/W2135235247","https://openalex.org/W2135590854","https://openalex.org/W2136701955","https://openalex.org/W2136802861","https://openalex.org/W2140992384","https://openalex.org/W2148054111","https://openalex.org/W2148405034","https://openalex.org/W2154575832","https://openalex.org/W2156539028","https://openalex.org/W2156639920","https://openalex.org/W2158500698","https://openalex.org/W2161551116","https://openalex.org/W2162977543","https://openalex.org/W2164176391","https://openalex.org/W2166848357","https://openalex.org/W2168799245","https://openalex.org/W2170405846","https://openalex.org/W2171223865","https://openalex.org/W2171443261","https://openalex.org/W2171938017","https://openalex.org/W2172029739","https://openalex.org/W2175687390","https://openalex.org/W2290094801","https://openalex.org/W2320556745","https://openalex.org/W2322067933","https://openalex.org/W2328508385","https://openalex.org/W2333404149","https://openalex.org/W2335679869","https://openalex.org/W2916426481","https://openalex.org/W2952965668","https://openalex.org/W3004426863","https://openalex.org/W3023173633","https://openalex.org/W3027294608","https://openalex.org/W3130325308","https://openalex.org/W3147248684","https://openalex.org/W4293253343"],"related_works":["https://openalex.org/W88987835","https://openalex.org/W1965706807","https://openalex.org/W2160559115","https://openalex.org/W1977041445","https://openalex.org/W1578789128","https://openalex.org/W4385263901","https://openalex.org/W1580630743","https://openalex.org/W157425379","https://openalex.org/W1989588669","https://openalex.org/W7014029777"],"abstract_inverted_index":{"Proteins":[0],"are":[1,28,65],"essential":[2],"components":[3],"in":[4,7,57,60,127,130,164,244,272,283,288,295,303,350,386],"most":[5],"processes":[6],"living":[8],"organisms.":[9],"The":[10,45,174,254,326],"detection":[11,125,410],"and":[12,27,129,206,234,265,270,315,323,363,377,398],"quantification":[13],"of":[14,23,30,38,47,73,146,156,197,218,238,310,320,336,400],"specific":[15,42,51],"proteins":[16,67],"can":[17,339,393],"be":[18,340,394],"used":[19,110,163,181,395],"e.g.":[20],"as":[21,91,182,208,282,405],"measures":[22],"certain":[24],"physiological":[25],"conditions,":[26],"therefore":[29],"great":[31],"importance.":[32],"This":[33],"thesis":[34,388],"focuses":[35],"on":[36,100,211,359],"development":[37],"affinity-based":[39],"bioassays":[40],"for":[41,50,123,186,250,317,396,408],"protein":[43,71,76,124,409],"detection.":[44],"use":[46],"Affibody":[48,63,97,117,153,198,220,256,278,313,337,371,406],"molecules":[49,64,118,199,279,314,338,407],"molecular":[52],"recognition":[53],"has":[54,108],"been":[55,109],"central":[56],"all":[58],"studies":[59,384],"this":[61,104,387],"thesis.":[62],"affinity":[66,184,202,402],"developed":[68,140],"by":[69,343],"combinatorial":[70],"engineering":[72],"the":[74,82,101,112,157,216,225,235,245,266,291,298,307,311,334,351,360,383],"58-residue":[75],"A-derived":[77],"Z":[78],"domain":[79],"scaffold.":[80],"In":[81,132,192,290],"first":[83],"paper,":[84,105],"solid":[85,190],"phase":[86],"peptide":[87,246,391],"synthesis":[88,107,217,362,392],"is":[89,332,356],"investigated":[90],"a":[92,165,189,195,239,273,357,368],"method":[93],"to":[94,115,141,169,224,248,260,305,348,366],"generate":[95,367],"functional":[96,121,149],"molecules.":[98,154],"Based":[99],"results":[102],"from":[103,329],"chemical":[106,144,361],"throughout":[111],"following":[113,292],"papers":[114,296],"produce":[116],"tailored":[119],"with":[120,252,373],"groups":[122],"applications":[126],"vitro":[128],"vivo.":[131],"paper":[133,193,354],"I,":[134],"an":[135,183,219],"orthogonal":[136],"protection":[137],"scheme":[138],"was":[139,177,180,258,268,301],"enable":[142],"site-specific":[143,236],"introduction":[145],"three":[147],"different":[148,201],"probes":[150,158],"into":[151],"synthetic":[152],"Two":[155],"were":[159,162,204,280],"fluorophores":[160],"that":[161,222,333],"FRET-based":[166],"binding":[167,263],"assay":[168],"detect":[170],"unlabeled":[171],"target":[172],"proteins.":[173],"third":[175],"probe":[176],"biotin,":[178],"which":[179],"handle":[185],"immobilization":[187],"onto":[188],"support.":[191],"II,":[194],"panel":[196],"carrying":[200],"handles":[203],"synthesized":[205],"evaluated":[207,281],"capture":[209],"ligands":[210],"microarrays.":[212],"Paper":[213],"III":[214],"describes":[215],"molecule":[221,257,372],"binds":[223],"human":[226],"epidermal":[227],"growth":[228],"factor":[229],"receptor":[230],"type":[231],"2,":[232],"(HER2),":[233],"incorporation":[237],"mercaptoacetyl-glycylglycylglycine":[240],"(MAG3)":[241],"chelating":[242],"site":[243],"sequence":[247,300],"allow":[249,316],"radiolabeling":[251],"99mTc.":[253],"derivatized":[255],"found":[259],"retain":[261],"its":[262],"capacity,":[264],"99mTc-labeling":[267],"efficient":[269],"resulted":[271],"stable":[274],"chelate":[275],"formation.":[276],"99mTc-labeled":[277],"vivo":[284],"HER2-targeting":[285],"imaging":[286,319],"agents":[287],"mice.":[289],"studies,":[293],"reported":[294],"IV-VI,":[297],"99mTc-chelating":[299],"engineered":[302],"order":[304],"optimize":[306],"pharmacokinetic":[308],"properties":[309],"radiolabeled":[312],"high-contrast":[318],"HER2-expressing":[321],"tumors":[322],"metastatic":[324],"lesions.":[325],"main":[327],"conclusion":[328],"these":[330],"investigations":[331],"biodistribution":[335],"dramatically":[341],"modified":[342],"amino":[344],"acid":[345],"substitutions":[346],"directed":[347],"residues":[349],"MAG3-chelator.":[352],"Finally,":[353],"VII":[355],"report":[358],"chemoselective":[364],"ligation":[365],"cross-linked":[369],"HER2-binding":[370],"improved":[374],"thermal":[375],"stability":[376],"tumor":[378],"targeting":[379],"capacity.":[380],"Taken":[381],"together,":[382],"presented":[385],"illustrate":[389],"how":[390],"production":[397],"modification":[399],"small":[401],"proteins,":[403],"such":[404],"applications.":[411]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}