{"id":"https://openalex.org/W2139701002","doi":"https://doi.org/10.1002/biot.201000233","title":"Increased expression of recombinant human tissue plasminogen activator in Leishmania tarentolae","display_name":"Increased expression of recombinant human tissue plasminogen activator in Leishmania tarentolae","publication_year":2010,"publication_date":"2010-11-01","ids":{"openalex":"https://openalex.org/W2139701002","doi":"https://doi.org/10.1002/biot.201000233","mag":"2139701002","pmid":"https://pubmed.ncbi.nlm.nih.gov/21058320"},"language":"en","primary_location":{"id":"doi:10.1002/biot.201000233","doi":"https://doi.org/10.1002/biot.201000233","is_oa":false,"landing_page_url":"https://doi.org/10.1002/biot.201000233","pdf_url":null,"source":{"id":"https://openalex.org/S31411064","display_name":"Biotechnology Journal","issn_l":"1860-6768","issn":["1860-6768","1860-7314"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology Journal","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5014259163","display_name":"Mahdi Hemayatkar","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1301411061","display_name":"Pasteur Institute of Iran","ror":"https://ror.org/00wqczk30","country_code":"IR","type":"facility","lineage":["https://openalex.org/I1301411061"]}],"countries":["IR"],"is_corresponding":false,"raw_author_name":"Mahdi Hemayatkar","raw_affiliation_strings":["Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran","institution_ids":["https://openalex.org/I1301411061"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5102931000","display_name":"Fereidoun Mahboudi","orcid":"https://orcid.org/0000-0003-3462-0403","observed_orcids":["https://orcid.org/0000-0003-3462-0403"]},"institutions":[{"id":"https://openalex.org/I1301411061","display_name":"Pasteur Institute of Iran","ror":"https://ror.org/00wqczk30","country_code":"IR","type":"facility","lineage":["https://openalex.org/I1301411061"]}],"countries":["IR"],"is_corresponding":false,"raw_author_name":"Fereidoun Mahboudi","raw_affiliation_strings":["Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran","institution_ids":["https://openalex.org/I1301411061"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5026819094","display_name":"Keivan Majidzadeh\u2010A","orcid":"https://orcid.org/0000-0002-8811-0997","observed_orcids":["https://orcid.org/0000-0002-8811-0997"]},"institutions":[{"id":"https://openalex.org/I1301411061","display_name":"Pasteur Institute of Iran","ror":"https://ror.org/00wqczk30","country_code":"IR","type":"facility","lineage":["https://openalex.org/I1301411061"]}],"countries":["IR"],"is_corresponding":false,"raw_author_name":"Keivan Majidzadeh\u2010A","raw_affiliation_strings":["Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran","institution_ids":["https://openalex.org/I1301411061"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5023526131","display_name":"Fatemeh Davami","orcid":"https://orcid.org/0000-0002-7693-2926","observed_orcids":["https://orcid.org/0000-0002-7693-2926"]},"institutions":[{"id":"https://openalex.org/I1301411061","display_name":"Pasteur Institute of Iran","ror":"https://ror.org/00wqczk30","country_code":"IR","type":"facility","lineage":["https://openalex.org/I1301411061"]}],"countries":["IR"],"is_corresponding":false,"raw_author_name":"Fatemeh Davami","raw_affiliation_strings":["Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran","institution_ids":["https://openalex.org/I1301411061"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5011350693","display_name":"Behrouz Vaziri","orcid":"https://orcid.org/0000-0002-0295-5922","observed_orcids":["https://orcid.org/0000-0002-0295-5922"]},"institutions":[{"id":"https://openalex.org/I1301411061","display_name":"Pasteur Institute of Iran","ror":"https://ror.org/00wqczk30","country_code":"IR","type":"facility","lineage":["https://openalex.org/I1301411061"]}],"countries":["IR"],"is_corresponding":false,"raw_author_name":"Behrouz Vaziri","raw_affiliation_strings":["Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran","institution_ids":["https://openalex.org/I1301411061"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5028326676","display_name":"Farzaneh Barkhordari","orcid":"https://orcid.org/0000-0003-0836-6712","observed_orcids":["https://orcid.org/0000-0003-0836-6712"]},"institutions":[{"id":"https://openalex.org/I1301411061","display_name":"Pasteur Institute of Iran","ror":"https://ror.org/00wqczk30","country_code":"IR","type":"facility","lineage":["https://openalex.org/I1301411061"]}],"countries":["IR"],"is_corresponding":false,"raw_author_name":"Farzaneh Barkhordari","raw_affiliation_strings":["Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran","institution_ids":["https://openalex.org/I1301411061"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100824925","display_name":"Ahmad Adeli","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1301411061","display_name":"Pasteur Institute of Iran","ror":"https://ror.org/00wqczk30","country_code":"IR","type":"facility","lineage":["https://openalex.org/I1301411061"]}],"countries":["IR"],"is_corresponding":false,"raw_author_name":"Ahmad Adeli","raw_affiliation_strings":["Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran","institution_ids":["https://openalex.org/I1301411061"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5078754068","display_name":"Reza Mahdian","orcid":"https://orcid.org/0000-0003-1458-4767","observed_orcids":["https://orcid.org/0000-0003-1458-4767"]},"institutions":[{"id":"https://openalex.org/I1301411061","display_name":"Pasteur Institute of Iran","ror":"https://ror.org/00wqczk30","country_code":"IR","type":"facility","lineage":["https://openalex.org/I1301411061"]}],"countries":["IR"],"is_corresponding":false,"raw_author_name":"Reza Mahdian","raw_affiliation_strings":["Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran","institution_ids":["https://openalex.org/I1301411061"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5046666712","display_name":"Noushin Davoudi","orcid":"https://orcid.org/0000-0001-6114-4188","observed_orcids":["https://orcid.org/0000-0001-6114-4188"]},"institutions":[{"id":"https://openalex.org/I1301411061","display_name":"Pasteur Institute of Iran","ror":"https://ror.org/00wqczk30","country_code":"IR","type":"facility","lineage":["https://openalex.org/I1301411061"]}],"countries":["IR"],"is_corresponding":true,"raw_author_name":"Noushin Davoudi","raw_affiliation_strings":["Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran","institution_ids":["https://openalex.org/I1301411061"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5046666712"],"corresponding_institution_ids":["https://openalex.org/I1301411061"],"apc_list":{"value":4780,"currency":"USD","value_usd":4780},"apc_paid":null,"fwci":0.9112,"has_fulltext":false,"cited_by_count":20,"citation_normalized_percentile":{"value":0.72331003,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":98},"biblio":{"volume":"5","issue":"11","first_page":"1198","last_page":"1206"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.9047999978065491,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.9047999978065491,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10823","display_name":"Research on Leishmaniasis Studies","score":0.04340000078082085,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12856","display_name":"Transgenic Plants and Applications","score":0.012299999594688416,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/alteplase","display_name":"alteplase","score":0.949999988079071},{"id":"https://openalex.org/keywords/leishmania-tarentolae","display_name":"Leishmania tarentolae","score":0.9980000257492065},{"id":"https://openalex.org/keywords/heterologous-expression","display_name":"heterologous expression","score":0.843999981880188},{"id":"https://openalex.org/keywords/thrombolytic-agents","display_name":"thrombolytic agents","score":0.9010000228881836},{"id":"https://openalex.org/keywords/serine-protease","display_name":"serine protease","score":0.8090000152587891},{"id":"https://openalex.org/keywords/gene-dosage","display_name":"gene dosage","score":0.656000018119812},{"id":"https://openalex.org/keywords/multiple-gene-copies","display_name":"multiple gene copies","score":0.6029999852180481},{"id":"https://openalex.org/keywords/electroporation-transformation","display_name":"electroporation transformation","score":0.7699999809265137}],"concepts":[{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.7148578763008118},{"id":"https://openalex.org/C108305142","wikidata":"https://www.wikidata.org/wiki/Q3062286","display_name":"Heterologous expression","level":4,"score":0.6647505164146423},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.6393997073173523},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5468648076057434},{"id":"https://openalex.org/C2779679481","wikidata":"https://www.wikidata.org/wiki/Q13581432","display_name":"Plasminogen activator","level":2,"score":0.5421519875526428},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.5255507230758667},{"id":"https://openalex.org/C2777807008","wikidata":"https://www.wikidata.org/wiki/Q87324999","display_name":"Serine protease","level":4,"score":0.51985102891922},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.5196336507797241},{"id":"https://openalex.org/C187882448","wikidata":"https://www.wikidata.org/wiki/Q283478","display_name":"Complementary DNA","level":3,"score":0.5038239359855652},{"id":"https://openalex.org/C2776415932","wikidata":"https://www.wikidata.org/wiki/Q48743","display_name":"Western blot","level":3,"score":0.4319491982460022},{"id":"https://openalex.org/C88045685","wikidata":"https://www.wikidata.org/wiki/Q174576","display_name":"Activator (genetics)","level":3,"score":0.4250164330005646},{"id":"https://openalex.org/C2776414213","wikidata":"https://www.wikidata.org/wiki/Q183290","display_name":"Serine","level":3,"score":0.4205528795719147},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.31639859080314636},{"id":"https://openalex.org/C2776714187","wikidata":"https://www.wikidata.org/wiki/Q212410","display_name":"Protease","level":3,"score":0.27450159192085266},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.1664642095565796},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.11380985379219055}],"mesh":[{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007891","descriptor_name":"Leishmania","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D007891","descriptor_name":"Leishmania","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D010959","descriptor_name":"Tissue Plasminogen Activator","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D010959","descriptor_name":"Tissue Plasminogen Activator","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D015153","descriptor_name":"Blotting, Western","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018274","descriptor_name":"Electroporation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018628","descriptor_name":"Gene Dosage","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D057005","descriptor_name":"Bioengineering","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1002/biot.201000233","doi":"https://doi.org/10.1002/biot.201000233","is_oa":false,"landing_page_url":"https://doi.org/10.1002/biot.201000233","pdf_url":null,"source":{"id":"https://openalex.org/S31411064","display_name":"Biotechnology Journal","issn_l":"1860-6768","issn":["1860-6768","1860-7314"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology Journal","raw_type":"journal-article"},{"id":"pmid:21058320","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/21058320","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology journal","raw_type":"Journal Article"},{"id":"pmh:oai:HAL:pasteur-00763505v1","doi":null,"is_oa":false,"landing_page_url":"https://riip.hal.science/pasteur-00763505","pdf_url":null,"source":{"id":"https://openalex.org/S4306402512","display_name":"HAL","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4405252976","host_organization_name":"Centre pour la Communication Scientifique Directe","host_organization_lineage":["https://openalex.org/I4405252976"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Biotechnology Journal, 2010, 5 (11), pp.1198-206. ⟨10.1002/biot.201000233⟩","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.8560000061988831}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":42,"referenced_works":["https://openalex.org/W39199943","https://openalex.org/W197919629","https://openalex.org/W331561824","https://openalex.org/W568573671","https://openalex.org/W1976513832","https://openalex.org/W2003816942","https://openalex.org/W2005343613","https://openalex.org/W2007901975","https://openalex.org/W2012527535","https://openalex.org/W2013627590","https://openalex.org/W2027971158","https://openalex.org/W2029091816","https://openalex.org/W2038431234","https://openalex.org/W2047186187","https://openalex.org/W2053314207","https://openalex.org/W2055566901","https://openalex.org/W2065503544","https://openalex.org/W2069105314","https://openalex.org/W2071247456","https://openalex.org/W2073920253","https://openalex.org/W2082581989","https://openalex.org/W2082917577","https://openalex.org/W2086194214","https://openalex.org/W2091780894","https://openalex.org/W2092603929","https://openalex.org/W2097536972","https://openalex.org/W2102565252","https://openalex.org/W2107277218","https://openalex.org/W2110403693","https://openalex.org/W2114874277","https://openalex.org/W2117510461","https://openalex.org/W2131285532","https://openalex.org/W2132537581","https://openalex.org/W2137755719","https://openalex.org/W2149034004","https://openalex.org/W2154346335","https://openalex.org/W2157768034","https://openalex.org/W2159463470","https://openalex.org/W2278222816","https://openalex.org/W2289462764","https://openalex.org/W2506548507","https://openalex.org/W4301893757"],"related_works":["https://openalex.org/W2245095135","https://openalex.org/W2159463470","https://openalex.org/W1993645771","https://openalex.org/W2142238493","https://openalex.org/W2969440610","https://openalex.org/W2040771434","https://openalex.org/W2064395839","https://openalex.org/W1979384534","https://openalex.org/W1528686707","https://openalex.org/W2003816942"],"abstract_inverted_index":{"Recombinant":[0],"tissue":[1],"plasminogen":[2],"activator":[3],"(rt-PA)":[4],"is":[5,22,167,179],"one":[6,84],"of":[7,27,31,39,65,74,88,109,113,133,143,162,172,177],"the":[8,96,107,131,139,159],"most":[9],"important":[10],"thrombolytic":[11],"agents":[12],"for":[13,47,93],"treating":[14],"cardiovascular":[15],"obstructions":[16],"such":[17],"as":[18],"stroke.":[19],"Glycoprotein":[20],"rt-PA":[21,115,163,178],"a":[23],"serine":[24],"protease,":[25],"consisting":[26],"527":[28],"amino":[29],"acids":[30],"which":[32],"35":[33],"are":[34],"cysteine":[35],"residues.":[36],"A":[37],"variety":[38],"recombinant":[40],"protein":[41],"expression":[42,50,73,81,160],"systems":[43],"have":[44],"been":[45,62],"developed":[46],"heterologous":[48],"gene":[49,136,145],"in":[51,72,106,138,164,187,190],"prokaryotic":[52],"and":[53,69,121,147,193],"eukaryotic":[54,196],"hosts.":[55],"In":[56,77],"recent":[57],"years,":[58],"Leishmania":[59],"tarentolae":[60,98,166,192],"has":[61],"considered":[63],"because":[64],"its":[66],"safety":[67],"aspects":[68],"special":[70],"attributes":[71],"complex":[75],"proteins.":[76],"this":[78],"study,":[79],"two":[80,86],"cassettes,":[82],"each":[83],"including":[85],"copies":[87,137],"t-PA":[89,135,144],"cDNA,":[90],"were":[91,104],"used":[92],"integration":[94],"into":[95],"L.":[97,165,191],"genome":[99],"by":[100,118],"electroporation.":[101],"Transformed":[102],"clones":[103],"selected":[105],"presence":[108,132],"appropriate":[110],"antibiotics.":[111],"Expression":[112],"active":[114],"was":[116,127,150,156,185],"confirmed":[117,151],"Western":[119],"blot":[120],"Zymography":[122],"tests.":[123],"Real-time":[124],"PCR":[125],"analysis":[126],"applied":[128],"to":[129],"investigate":[130],"multiple":[134],"parasite":[140],"genome.":[141],"Correlation":[142],"dosage":[146],"production":[148],"rate":[149],"with":[152],"real-time":[153],"PCR.":[154],"It":[155],"shown":[157],"that":[158],"level":[161],"at":[168],"least":[169],"480":[170],"IU/mL":[171],"culture":[173],"media.":[174],"This":[175],"concentration":[176],"seven":[180],"times":[181],"higher":[182],"than":[183],"what":[184],"reported":[186],"previous":[188],"studies":[189],"some":[194],"other":[195],"systems.":[197]},"counts_by_year":[{"year":2023,"cited_by_count":1},{"year":2019,"cited_by_count":4},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":3},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}