{"id":"https://openalex.org/W2139235398","doi":"https://doi.org/10.1128/.38.6.2429-2433.2000","title":"Rapid Identification of Methicillin-ResistantStaphylococcus aureus and Simultaneous Species Confirmation Using Real-Time Fluorescence PCR","display_name":"Rapid Identification of Methicillin-ResistantStaphylococcus aureus and Simultaneous Species Confirmation Using Real-Time Fluorescence PCR","publication_year":2000,"publication_date":"2000-01-01","ids":{"openalex":"https://openalex.org/W2139235398","doi":"https://doi.org/10.1128/.38.6.2429-2433.2000","mag":"2139235398","pmid":"https://pubmed.ncbi.nlm.nih.gov/10835024","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/86833"},"language":"en","primary_location":{"id":"doi:10.1128/.38.6.2429-2433.2000","doi":"https://doi.org/10.1128/.38.6.2429-2433.2000","is_oa":false,"landing_page_url":"https://doi.org/10.1128/.38.6.2429-2433.2000","pdf_url":null,"source":{"id":"https://openalex.org/S53087977","display_name":"Journal of Clinical Microbiology","issn_l":"0095-1137","issn":["0095-1137","1070-633X","1098-660X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Clinical Microbiology","raw_type":"journal-article"},"parent_works":[],"lineage":["https://openalex.org/W2139235398"],"child_works_count":0,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://jcm.asm.org/content/jcm/38/6/2429.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5076106872","display_name":"Udo Reischl","orcid":"https://orcid.org/0000-0002-7536-5018","observed_orcids":["https://orcid.org/0000-0002-7536-5018"]},"institutions":[{"id":"https://openalex.org/I4210140742","display_name":"Institute of Medical Microbiology and Hygiene","ror":"https://ror.org/04xqmb911","country_code":"DE","type":"facility","lineage":["https://openalex.org/I4210140742"]},{"id":"https://openalex.org/I60668342","display_name":"University of Regensburg","ror":"https://ror.org/01eezs655","country_code":"DE","type":"education","lineage":["https://openalex.org/I60668342"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Udo Reischl","raw_affiliation_strings":["Institute of Medical Microbiology and Hygiene, University of Regensburg, D-93053 Regensburg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Medical Microbiology and Hygiene, University of Regensburg, D-93053 Regensburg, Germany","institution_ids":["https://openalex.org/I4210140742","https://openalex.org/I60668342"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113625951","display_name":"Hans\u2010J\u00f6rg Linde","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210140742","display_name":"Institute of Medical Microbiology and Hygiene","ror":"https://ror.org/04xqmb911","country_code":"DE","type":"facility","lineage":["https://openalex.org/I4210140742"]},{"id":"https://openalex.org/I60668342","display_name":"University of Regensburg","ror":"https://ror.org/01eezs655","country_code":"DE","type":"education","lineage":["https://openalex.org/I60668342"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Hans-Jo\u0308rg Linde","raw_affiliation_strings":["Institute of Medical Microbiology and Hygiene, University of Regensburg, D-93053 Regensburg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Medical Microbiology and Hygiene, University of Regensburg, D-93053 Regensburg, Germany","institution_ids":["https://openalex.org/I4210140742","https://openalex.org/I60668342"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5051952062","display_name":"Michaela Metz","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210140742","display_name":"Institute of Medical Microbiology and Hygiene","ror":"https://ror.org/04xqmb911","country_code":"DE","type":"facility","lineage":["https://openalex.org/I4210140742"]},{"id":"https://openalex.org/I60668342","display_name":"University of Regensburg","ror":"https://ror.org/01eezs655","country_code":"DE","type":"education","lineage":["https://openalex.org/I60668342"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Michaela Metz","raw_affiliation_strings":["Institute of Medical Microbiology and Hygiene, University of Regensburg, D-93053 Regensburg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Medical Microbiology and Hygiene, University of Regensburg, D-93053 Regensburg, Germany","institution_ids":["https://openalex.org/I4210140742","https://openalex.org/I60668342"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5068084481","display_name":"Birgit Leppmeier","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210140742","display_name":"Institute of Medical Microbiology and Hygiene","ror":"https://ror.org/04xqmb911","country_code":"DE","type":"facility","lineage":["https://openalex.org/I4210140742"]},{"id":"https://openalex.org/I60668342","display_name":"University of Regensburg","ror":"https://ror.org/01eezs655","country_code":"DE","type":"education","lineage":["https://openalex.org/I60668342"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Birgit Leppmeier","raw_affiliation_strings":["Institute of Medical Microbiology and Hygiene, University of Regensburg, D-93053 Regensburg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Medical Microbiology and Hygiene, University of Regensburg, D-93053 Regensburg, Germany","institution_ids":["https://openalex.org/I4210140742","https://openalex.org/I60668342"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5091851420","display_name":"Norbert Lehn","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210140742","display_name":"Institute of Medical Microbiology and Hygiene","ror":"https://ror.org/04xqmb911","country_code":"DE","type":"facility","lineage":["https://openalex.org/I4210140742"]},{"id":"https://openalex.org/I60668342","display_name":"University of Regensburg","ror":"https://ror.org/01eezs655","country_code":"DE","type":"education","lineage":["https://openalex.org/I60668342"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Norbert Lehn","raw_affiliation_strings":["Institute of Medical Microbiology and Hygiene, University of Regensburg, D-93053 Regensburg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Medical Microbiology and Hygiene, University of Regensburg, D-93053 Regensburg, Germany","institution_ids":["https://openalex.org/I4210140742","https://openalex.org/I60668342"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":7.6416,"has_fulltext":false,"cited_by_count":219,"citation_normalized_percentile":{"value":0.97750404,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":91,"max":99},"biblio":{"volume":"38","issue":"6","first_page":"2429","last_page":"2433"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10195","display_name":"Antimicrobial Resistance in Staphylococcus","score":0.9965999722480774,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10195","display_name":"Antimicrobial Resistance in Staphylococcus","score":0.9965999722480774,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12167","display_name":"Bacterial Identification and Susceptibility Testing","score":0.0032999999821186066,"subfield":{"id":"https://openalex.org/subfields/2726","display_name":"Microbiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12824","display_name":"Infections and bacterial resistance","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2726","display_name":"Microbiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/methicillin-resistant-staphylococcus-aureus","display_name":"methicillin-resistant Staphylococcus aureus","score":0.9990000128746033},{"id":"https://openalex.org/keywords/meca","display_name":"mecA gene","score":0.8640000224113464},{"id":"https://openalex.org/keywords/duplex-real-time-pcr","display_name":"duplex real-time PCR","score":0.75},{"id":"https://openalex.org/keywords/rapid-bacterial-identification","display_name":"rapid bacterial identification","score":0.6340000033378601},{"id":"https://openalex.org/keywords/antimicrobial-resistance-detection","display_name":"antimicrobial resistance detection","score":0.5989999771118164}],"concepts":[{"id":"https://openalex.org/C2779489039","wikidata":"https://www.wikidata.org/wiki/Q188121","display_name":"Staphylococcus aureus","level":3,"score":0.9293121099472046},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.7130838632583618},{"id":"https://openalex.org/C2775965595","wikidata":"https://www.wikidata.org/wiki/Q1365581","display_name":"Micrococcaceae","level":4,"score":0.6550903916358948},{"id":"https://openalex.org/C11701158","wikidata":"https://www.wikidata.org/wiki/Q7389099","display_name":"SCCmec","level":5,"score":0.6177288293838501},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6174198389053345},{"id":"https://openalex.org/C48023723","wikidata":"https://www.wikidata.org/wiki/Q856198","display_name":"Real-time polymerase chain reaction","level":3,"score":0.5562856197357178},{"id":"https://openalex.org/C2779288629","wikidata":"https://www.wikidata.org/wiki/Q199695","display_name":"Staphylococcus","level":4,"score":0.4835245609283447},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.4659707546234131},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.45386409759521484},{"id":"https://openalex.org/C99611785","wikidata":"https://www.wikidata.org/wiki/Q6453233","display_name":"Duplex (building)","level":3,"score":0.43842780590057373},{"id":"https://openalex.org/C2777334864","wikidata":"https://www.wikidata.org/wiki/Q34879","display_name":"Staphylococcal infections","level":4,"score":0.4129217267036438},{"id":"https://openalex.org/C2777052132","wikidata":"https://www.wikidata.org/wiki/Q595158","display_name":"Methicillin-resistant Staphylococcus aureus","level":4,"score":0.3499763011932373},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.3367452025413513},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.12692871689796448},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.11110585927963257}],"mesh":[{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D002267","descriptor_name":"Muramoylpentapeptide Carboxypeptidase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D002352","descriptor_name":"Carrier Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D005453","descriptor_name":"Fluorescence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006602","descriptor_name":"Hexosyltransferases","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D010458","descriptor_name":"Peptidyl Transferases","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012680","descriptor_name":"Sensitivity and Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013211","descriptor_name":"Staphylococcus aureus","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D013211","descriptor_name":"Staphylococcus aureus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D013211","descriptor_name":"Staphylococcus aureus","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D016106","descriptor_name":"Methicillin Resistance","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":"Q000295","qualifier_name":"instrumentation","is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D046915","descriptor_name":"Penicillin-Binding Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":5,"locations":[{"id":"doi:10.1128/.38.6.2429-2433.2000","doi":"https://doi.org/10.1128/.38.6.2429-2433.2000","is_oa":false,"landing_page_url":"https://doi.org/10.1128/.38.6.2429-2433.2000","pdf_url":null,"source":{"id":"https://openalex.org/S53087977","display_name":"Journal of Clinical Microbiology","issn_l":"0095-1137","issn":["0095-1137","1070-633X","1098-660X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Clinical Microbiology","raw_type":"journal-article"},{"id":"doi:10.1128/jcm.38.6.2429-2433.2000","doi":"https://doi.org/10.1128/jcm.38.6.2429-2433.2000","is_oa":true,"landing_page_url":"https://doi.org/10.1128/jcm.38.6.2429-2433.2000","pdf_url":"https://jcm.asm.org/content/jcm/38/6/2429.full.pdf","source":{"id":"https://openalex.org/S53087977","display_name":"Journal of Clinical Microbiology","issn_l":"0095-1137","issn":["0095-1137","1070-633X","1098-660X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Clinical Microbiology","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:203078","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC86833","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},{"id":"pmid:10835024","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10835024","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of clinical microbiology","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:86833","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC86833/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Clin Microbiol","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1128/jcm.38.6.2429-2433.2000","doi":"https://doi.org/10.1128/jcm.38.6.2429-2433.2000","is_oa":true,"landing_page_url":"https://doi.org/10.1128/jcm.38.6.2429-2433.2000","pdf_url":"https://jcm.asm.org/content/jcm/38/6/2429.full.pdf","source":{"id":"https://openalex.org/S53087977","display_name":"Journal of Clinical Microbiology","issn_l":"0095-1137","issn":["0095-1137","1070-633X","1098-660X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Clinical Microbiology","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.5683000087738037,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":false},"content_urls":{"pdf":"https://content.openalex.org/works/W2139235398.pdf"},"referenced_works_count":23,"referenced_works":["https://openalex.org/W53930108","https://openalex.org/W1556616287","https://openalex.org/W1624098586","https://openalex.org/W1831808847","https://openalex.org/W1916238341","https://openalex.org/W1983767131","https://openalex.org/W1984666403","https://openalex.org/W2028304505","https://openalex.org/W2036989796","https://openalex.org/W2054350590","https://openalex.org/W2100883347","https://openalex.org/W2113091465","https://openalex.org/W2121784940","https://openalex.org/W2123689798","https://openalex.org/W2133043345","https://openalex.org/W2139378390","https://openalex.org/W2148735060","https://openalex.org/W2148872503","https://openalex.org/W2150058124","https://openalex.org/W2153965747","https://openalex.org/W2157170054","https://openalex.org/W2166306225","https://openalex.org/W2314649741"],"related_works":["https://openalex.org/W2376565187","https://openalex.org/W2055883538","https://openalex.org/W2045496013","https://openalex.org/W1979498904","https://openalex.org/W2371062592","https://openalex.org/W2348800003","https://openalex.org/W2144394067","https://openalex.org/W1988218567","https://openalex.org/W2081191412","https://openalex.org/W2114764103"],"abstract_inverted_index":{"A":[0],"duplex":[1],"LightCycler":[2],"PCR":[3],"assay":[4,31],"targeting":[5],"the":[6,47,52],"mecA":[7,53],"gene":[8,54],"and":[9,22],"a":[10],"Staphylococcus":[11],"aureus-specific":[12],"marker":[13],"was":[14,55],"used":[15],"to":[16],"test":[17],"165":[18],"S.":[19,42],"aureus":[20,43],"strains":[21,24],"80":[23],"of":[25,33,51],"other":[26],"bacterial":[27],"species.":[28],"Within":[29],"an":[30],"time":[32],"60":[34],"min":[35,38],"plus":[36],"10":[37],"for":[39],"sample":[40],"preparation,":[41],"as":[44,46],"well":[45],"presence":[48],"or":[49],"absence":[50],"correctly":[56],"identified.":[57]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":3},{"year":2024,"cited_by_count":4},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":6},{"year":2021,"cited_by_count":10},{"year":2020,"cited_by_count":11},{"year":2019,"cited_by_count":4},{"year":2018,"cited_by_count":13},{"year":2017,"cited_by_count":7},{"year":2016,"cited_by_count":5},{"year":2015,"cited_by_count":8},{"year":2014,"cited_by_count":12},{"year":2013,"cited_by_count":4},{"year":2012,"cited_by_count":8}],"updated_date":"2026-11-14T11:04:58.165337","created_date":"2016-06-24T00:00:00"}